FastQCFastQC Report
Sat 18 Jun 2016
SRR3551591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602058
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.40826631321234835No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC11820.19632659976281355Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC8620.14317557444631582Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT7870.13071830288776165Illumina PCR Primer Index 11 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7760.12889123639250705No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7390.12274564909028698No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC7070.1174305465586372No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6980.1159356739716107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG406.8066583E-945.0000041
TTAATCG600.045.00000420
TCGGACG207.0306065E-445.0000041
CGTTTGA207.0306065E-445.00000423
TGCGTTG207.0306065E-445.0000041
TAATCGT600.045.00000421
ACGAAAG207.0306065E-445.0000041
GTTTACG253.8885853E-545.01
GACGTAG253.8885853E-545.01
TAACGCC551.8189894E-1244.99999612
ATAACGC551.8189894E-1244.99999611
CGAATAT1650.042.27272814
TACGAAT1600.042.18750412
CGCATCG603.6379788E-1241.25000421
CTCACGA501.0804797E-940.524
TCACGAC451.925764E-840.025
TGACTAT1700.039.70588323
AGGGTAC2050.039.5121966
CGTAAGG403.45457E-739.3750042
TCGTTGA700.038.57142624