##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	602058
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.073869959372686	33.0	31.0	34.0	30.0	34.0
2	32.16564849233795	33.0	31.0	34.0	30.0	34.0
3	32.126775161197095	33.0	31.0	34.0	30.0	34.0
4	35.75064196472765	37.0	35.0	37.0	35.0	37.0
5	35.793564739609806	37.0	35.0	37.0	35.0	37.0
6	35.67359457062276	37.0	35.0	37.0	35.0	37.0
7	35.98742313863448	37.0	35.0	37.0	35.0	37.0
8	35.804688252626825	37.0	35.0	37.0	35.0	37.0
9	37.64567035069711	39.0	38.0	39.0	35.0	39.0
10	37.26887941028937	39.0	37.0	39.0	34.0	39.0
11	37.316464526673506	39.0	37.0	39.0	34.0	39.0
12	37.237546880865295	39.0	37.0	39.0	34.0	39.0
13	37.32410830850184	39.0	37.0	39.0	34.0	39.0
14	38.41744150895761	40.0	38.0	41.0	34.0	41.0
15	38.517348826857216	40.0	38.0	41.0	34.0	41.0
16	38.24411601540051	40.0	37.0	41.0	34.0	41.0
17	38.285859501908455	40.0	37.0	41.0	34.0	41.0
18	38.108384906437585	40.0	37.0	41.0	34.0	41.0
19	37.91979011988878	40.0	37.0	41.0	33.0	41.0
20	38.2329526391145	40.0	37.0	41.0	34.0	41.0
21	38.06779911569981	40.0	37.0	41.0	34.0	41.0
22	38.22487202229686	40.0	37.0	41.0	34.0	41.0
23	38.26869836460939	40.0	37.0	41.0	34.0	41.0
24	38.1498726036362	40.0	37.0	41.0	34.0	41.0
25	37.75176145819838	40.0	36.0	41.0	33.0	41.0
26	37.94720110022622	40.0	37.0	41.0	34.0	41.0
27	38.035680947682785	40.0	37.0	41.0	34.0	41.0
28	37.94493055486348	40.0	36.0	41.0	34.0	41.0
29	37.95965006693707	40.0	36.0	41.0	34.0	41.0
30	37.691680867956244	40.0	36.0	41.0	33.0	41.0
31	37.62274897102937	40.0	36.0	41.0	33.0	41.0
32	37.67845456750014	40.0	36.0	41.0	33.0	41.0
33	37.66337794697521	40.0	36.0	41.0	33.0	41.0
34	37.55959558713612	40.0	36.0	41.0	33.0	41.0
35	37.50860216125357	40.0	36.0	41.0	33.0	41.0
36	37.33936597470676	40.0	35.0	41.0	33.0	41.0
37	37.30993027249866	40.0	35.0	41.0	33.0	41.0
38	37.13283770002226	39.0	35.0	41.0	33.0	41.0
39	37.07061445907205	39.0	35.0	41.0	32.0	41.0
40	36.95719017104664	39.0	35.0	41.0	32.0	41.0
41	36.78117888974152	39.0	35.0	41.0	32.0	41.0
42	36.84579060489189	39.0	35.0	41.0	32.0	41.0
43	36.81278714010943	39.0	35.0	41.0	32.0	41.0
44	36.78941563769604	39.0	35.0	41.0	32.0	41.0
45	36.73509861176166	39.0	35.0	41.0	32.0	41.0
46	36.62163944337589	39.0	35.0	41.0	32.0	41.0
47	36.55652113251547	39.0	35.0	41.0	32.0	41.0
48	36.478327669427195	38.0	35.0	41.0	32.0	41.0
49	36.42628451079464	38.0	35.0	40.0	31.0	41.0
50	36.2971457899405	38.0	35.0	40.0	31.0	41.0
51	35.38450946586541	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	32.0
10	38.0
11	23.0
12	47.0
13	30.0
14	41.0
15	71.0
16	90.0
17	141.0
18	191.0
19	304.0
20	442.0
21	706.0
22	991.0
23	1467.0
24	2185.0
25	3019.0
26	4059.0
27	4920.0
28	5400.0
29	6313.0
30	7517.0
31	9385.0
32	12361.0
33	18001.0
34	35081.0
35	46133.0
36	48389.0
37	75830.0
38	130630.0
39	188092.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.176929797461376	21.570845333838268	24.807742775612983	12.444482093087377
2	33.49976248135562	24.657923323002105	24.668719625019516	17.173594570622765
3	33.178032681236694	23.192283799899677	26.594447711017878	17.035235807845755
4	30.45221556727093	25.138940102116408	25.18660992794714	19.222234402665524
5	25.985868471143974	29.763909789422282	23.451062854409376	20.799158885024365
6	27.648000690963332	33.951712293499966	23.26038355108644	15.139903464450269
7	80.01205863886868	6.3995495450604425	8.758292390434145	4.830099425636733
8	80.71248949436766	5.815054363533082	8.399190775639557	5.07326536645971
9	74.13355523886403	8.02497433802059	11.047440612034057	6.794029811081324
10	39.872736513757815	25.950655916871796	17.68052247457886	16.496085094791532
11	30.27831205631351	24.102495108444703	24.42439100551774	21.194801829724046
12	26.76220563467307	21.651734550491813	30.206225978228012	21.379833836607105
13	24.915871892741233	22.775214348119285	30.947848878347266	21.361064880792217
14	22.219121745745426	26.041843144680417	28.7631756408851	22.975859468689062
15	21.55290686279395	24.046188241000035	31.517395334004366	22.88350956220165
16	23.346421773317523	22.57191167628368	31.16825953645662	22.913407013942177
17	22.83600583332503	23.511356048752777	27.51944164847905	26.133196469443142
18	24.045025562321236	23.568659497922127	28.62282371465872	23.763491225097912
19	25.624607595945903	24.617063472290045	26.329855263114183	23.428473668649865
20	25.890861013390737	24.381205797448086	26.420212006152234	23.307721183008944
21	25.604177670589877	25.172325589893337	26.61687079982327	22.60662593969352
22	24.4120998309133	23.077510804606867	25.61115374266267	26.89923562181717
23	23.6498809084839	24.830664155280722	26.458580402552577	25.060874533682803
24	23.89072149194928	25.126150636649626	27.484727385069213	23.49840048633188
25	24.61041959412548	24.900923166870967	26.26208770583565	24.2265695331679
26	23.073524477708126	26.288995412402127	25.73904839733049	24.898431712559255
27	24.513751166831103	24.631846101206197	27.760116134990316	23.094286596972385
28	23.526470871577157	25.25637064867505	27.87621790591604	23.340940573831755
29	24.629852937756826	24.94659982925233	26.302449265685368	24.121097967305474
30	24.792960146696828	24.451298712084217	27.013510326247637	23.742230814971315
31	25.44555507941095	25.331280374980487	25.0832976224882	24.139866923120366
32	24.757913689378764	26.476684970551013	24.738646442701533	24.026754897368694
33	24.701772918888214	25.028651724584673	24.870527424268094	25.399047932259016
34	23.378312388507418	25.405857907377694	26.007461075178806	25.208368628936086
35	25.060708436728685	24.455285038982954	25.756654674466578	24.727351849821776
36	25.25304870959276	25.441070461649872	24.913048244521292	24.39283258423607
37	23.11039800152145	27.096226609396435	26.407588637639563	23.385786751442552
38	23.36469243827007	26.738952061097105	25.48757760880181	24.40877789183102
39	23.746881529686508	24.479369097329492	25.460337708327103	26.313411664656893
40	24.298821708207516	23.867301821419197	27.711117533526668	24.122758936846616
41	22.310973361370497	24.636330718967276	26.91036411774281	26.142331801919415
42	23.74106813629252	25.154553215803126	26.86119941932505	24.243179228579308
43	23.743725687558342	23.01007544123656	27.54319351291736	25.70300535828774
44	23.8141507961027	24.44066850702092	26.91783848067794	24.82734221619844
45	23.675459839417464	23.843383860026776	26.621023223676126	25.860133076879638
46	24.321410893967027	24.310282398041387	27.036597802869487	24.3317089051221
47	23.30622631042192	25.05423065551824	27.30318341422255	24.336359619837292
48	23.050104807178045	24.4959787927409	27.082606659159087	25.371309740921973
49	22.920715279923197	24.54647226679157	27.779217284713432	24.7535951685718
50	22.34087081311103	23.333466210896624	28.62481687810809	25.700846097884256
51	22.86474060638676	24.549628108919737	25.83023562513911	26.75539565955439
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	790.0
2	1284.0
3	2670.5
4	4057.0
5	2788.5
6	1520.0
7	1501.0
8	1482.0
9	1471.5
10	1461.0
11	1432.5
12	1404.0
13	1426.5
14	1449.0
15	1380.5
16	1312.0
17	1213.5
18	1115.0
19	1308.5
20	1502.0
21	1391.5
22	1281.0
23	1593.5
24	1906.0
25	1991.0
26	2916.0
27	3756.0
28	4866.0
29	5976.0
30	7339.0
31	8702.0
32	8949.5
33	9197.0
34	10550.0
35	11903.0
36	12442.5
37	12982.0
38	14284.0
39	15586.0
40	18071.5
41	20557.0
42	23899.0
43	27241.0
44	30557.0
45	33873.0
46	39333.5
47	44794.0
48	49883.5
49	54973.0
50	54060.5
51	53148.0
52	47489.5
53	41831.0
54	39422.5
55	37014.0
56	35200.5
57	33387.0
58	31991.0
59	30595.0
60	30001.0
61	29407.0
62	27810.0
63	26213.0
64	23602.0
65	20991.0
66	18547.0
67	16103.0
68	14266.5
69	12430.0
70	11452.0
71	10474.0
72	9175.5
73	7877.0
74	6340.5
75	3775.5
76	2747.0
77	2300.0
78	1853.0
79	1320.5
80	788.0
81	613.5
82	439.0
83	305.5
84	172.0
85	128.5
86	85.0
87	48.0
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	602058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.054381133699746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45574514718314	21.051568348324746
2	8.399646912564714	4.880930855723192
3	3.0522823866475624	2.660465273680109
4	1.7050876577871767	1.981610667028642
5	1.2602612166112455	1.830805485772163
6	0.9971043096899109	1.7382149186271159
7	0.8868074995329218	1.8035950158556942
8	0.7288055278279018	1.6939994862287262
9	0.7170649184565268	1.8750489694597134
>10	9.647117383534344	54.13011338593279
>50	0.09085123504214501	1.764206005257721
>100	0.05175070350501911	2.7660945860844692
>500	0.006325085983946779	1.2152305380913468
>1k	0.0011500156334448691	0.6081164639335307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2458	0.40826631321234835	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1182	0.19632659976281355	Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	862	0.14317557444631582	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	787	0.13071830288776165	Illumina PCR Primer Index 11 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	776	0.12889123639250705	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	739	0.12274564909028698	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	707	0.1174305465586372	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	698	0.1159356739716107	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6609695411405547E-4	0.0	0.0	0.13603340541941142	0.0
2	1.6609695411405547E-4	0.0	0.0	0.6125655667726365	0.0
3	1.6609695411405547E-4	0.0	0.0	0.8907779649136794	0.0
4	1.6609695411405547E-4	0.0	0.0	1.2779499649535426	0.0
5	3.3219390822811093E-4	0.0	0.0	2.339807792604699	0.0
6	3.3219390822811093E-4	0.0	0.0	3.1347478149945687	0.0
7	3.3219390822811093E-4	0.0	0.0	3.8110946121470026	0.0
8	3.3219390822811093E-4	0.0	0.0	4.938892930581439	0.0
9	3.3219390822811093E-4	0.0	0.0	5.434858435566008	0.0
10	3.3219390822811093E-4	0.0	0.0	6.323975430938548	0.0
11	3.3219390822811093E-4	0.0	0.0	7.502433320377771	0.0
12	3.3219390822811093E-4	0.0	0.0	8.322121788930634	0.0
13	3.3219390822811093E-4	0.0	0.0	8.651325951984692	0.0
14	3.3219390822811093E-4	0.0	0.0	8.777393540157261	0.0
15	3.3219390822811093E-4	0.0	0.0	9.0378335642081	0.0
16	3.3219390822811093E-4	0.0	0.0	9.55389680064047	0.0
17	3.3219390822811093E-4	0.0	0.0	10.16911991867893	0.0
18	3.3219390822811093E-4	0.0	0.0	10.856761308711121	0.0
19	3.3219390822811093E-4	0.0	0.0	11.215364632643366	0.0
20	3.3219390822811093E-4	0.0	0.0	11.58376767686834	0.0
21	3.3219390822811093E-4	0.0	0.0	12.021765344867106	0.0
22	3.3219390822811093E-4	0.0	0.0	12.445146480903833	0.0
23	3.3219390822811093E-4	0.0	0.0	12.917027927541865	0.0
24	3.3219390822811093E-4	0.0	0.0	13.288586813895007	0.0
25	3.3219390822811093E-4	0.0	0.0	13.601513475445888	0.0
26	3.3219390822811093E-4	0.0	0.0	13.895338987273652	0.0
27	3.3219390822811093E-4	0.0	0.0	14.192154244275468	0.0
28	3.3219390822811093E-4	0.0	0.0	14.53298519411751	0.0
29	3.3219390822811093E-4	0.0	0.0	14.860362290676314	0.0
30	3.3219390822811093E-4	0.0	0.0	15.23291775875414	0.0
31	3.3219390822811093E-4	0.0	0.0	15.566108248706936	0.0
32	3.3219390822811093E-4	0.0	0.0	15.910925525447714	0.0
33	3.3219390822811093E-4	0.0	0.0	16.234814585970124	0.0
34	3.3219390822811093E-4	0.0	0.0	16.55405293177734	0.0
35	3.3219390822811093E-4	0.0	0.0	16.902690438462738	0.0
36	3.3219390822811093E-4	0.0	0.0	17.212627354839565	0.0
37	3.3219390822811093E-4	0.0	0.0	17.551299044278124	0.0
38	3.3219390822811093E-4	0.0	0.0	17.863727414966664	0.0
39	3.3219390822811093E-4	0.0	0.0	18.18047430646217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	40	6.8066583E-9	45.000004	1
TTAATCG	60	0.0	45.000004	20
TCGGACG	20	7.0306065E-4	45.000004	1
CGTTTGA	20	7.0306065E-4	45.000004	23
TGCGTTG	20	7.0306065E-4	45.000004	1
TAATCGT	60	0.0	45.000004	21
ACGAAAG	20	7.0306065E-4	45.000004	1
GTTTACG	25	3.8885853E-5	45.0	1
GACGTAG	25	3.8885853E-5	45.0	1
TAACGCC	55	1.8189894E-12	44.999996	12
ATAACGC	55	1.8189894E-12	44.999996	11
CGAATAT	165	0.0	42.272728	14
TACGAAT	160	0.0	42.187504	12
CGCATCG	60	3.6379788E-12	41.250004	21
CTCACGA	50	1.0804797E-9	40.5	24
TCACGAC	45	1.925764E-8	40.0	25
TGACTAT	170	0.0	39.705883	23
AGGGTAC	205	0.0	39.512196	6
CGTAAGG	40	3.45457E-7	39.375004	2
TCGTTGA	70	0.0	38.571426	24
>>END_MODULE