##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548703
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02320927714993	33.0	31.0	34.0	30.0	34.0
2	32.14796529269933	33.0	31.0	34.0	30.0	34.0
3	32.05349341993756	33.0	31.0	34.0	30.0	34.0
4	35.69189525116502	37.0	35.0	37.0	33.0	37.0
5	35.774222120163365	37.0	35.0	37.0	35.0	37.0
6	35.674167992520545	37.0	35.0	37.0	33.0	37.0
7	35.95988358000594	37.0	35.0	37.0	35.0	37.0
8	35.80200764347926	37.0	35.0	37.0	35.0	37.0
9	37.63457097920004	39.0	37.0	39.0	35.0	39.0
10	37.34819929907436	39.0	37.0	39.0	34.0	39.0
11	37.41722571227057	39.0	37.0	39.0	35.0	39.0
12	37.35726066742846	39.0	37.0	39.0	35.0	39.0
13	37.46018337789296	39.0	37.0	39.0	35.0	39.0
14	38.58593264480056	40.0	38.0	41.0	35.0	41.0
15	38.65900131765272	40.0	38.0	41.0	35.0	41.0
16	38.40827916012852	40.0	38.0	41.0	34.0	41.0
17	38.41843766117554	40.0	38.0	41.0	34.0	41.0
18	38.11182734557675	39.0	37.0	41.0	34.0	41.0
19	37.78384845717993	39.0	37.0	41.0	33.0	41.0
20	37.96206691051443	39.0	35.0	41.0	34.0	41.0
21	37.778576023823454	39.0	35.0	41.0	34.0	41.0
22	37.93298742671354	40.0	35.0	41.0	34.0	41.0
23	37.975666252963805	40.0	35.0	41.0	34.0	41.0
24	37.81656743265482	40.0	35.0	41.0	34.0	41.0
25	37.44475608844858	39.0	35.0	41.0	33.0	41.0
26	37.62109192040138	39.0	35.0	41.0	33.0	41.0
27	37.71773254383519	40.0	35.0	41.0	34.0	41.0
28	37.59565557323361	40.0	35.0	41.0	33.0	41.0
29	37.57380404335314	40.0	35.0	41.0	33.0	41.0
30	37.23025753458611	39.0	35.0	41.0	33.0	41.0
31	37.03862198675786	39.0	35.0	41.0	33.0	41.0
32	36.851571797493364	39.0	35.0	41.0	32.0	41.0
33	36.58320074794561	39.0	35.0	41.0	31.0	41.0
34	36.264407156512725	39.0	35.0	41.0	30.0	41.0
35	36.08183297703858	39.0	35.0	41.0	29.0	41.0
36	35.872829199038456	39.0	35.0	41.0	28.0	41.0
37	35.837919603136854	39.0	35.0	41.0	27.0	41.0
38	35.65934394380931	39.0	35.0	41.0	26.0	41.0
39	35.565110815869424	39.0	35.0	41.0	26.0	41.0
40	35.477433146893674	39.0	35.0	41.0	26.0	41.0
41	35.297036830489354	38.0	35.0	40.0	24.0	41.0
42	35.29310574208634	39.0	35.0	41.0	24.0	41.0
43	35.28275405820635	38.0	35.0	41.0	24.0	41.0
44	35.29146004304697	38.0	35.0	41.0	24.0	41.0
45	35.2510101092941	38.0	35.0	41.0	24.0	41.0
46	35.18380070821556	38.0	35.0	40.0	24.0	41.0
47	35.13491451659641	38.0	35.0	40.0	24.0	41.0
48	35.06258394796456	38.0	35.0	40.0	24.0	41.0
49	35.000778198770554	37.0	35.0	40.0	24.0	41.0
50	34.887522029221635	37.0	35.0	40.0	24.0	41.0
51	33.9967778561444	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	19.0
9	27.0
10	28.0
11	22.0
12	21.0
13	23.0
14	38.0
15	54.0
16	88.0
17	114.0
18	185.0
19	339.0
20	596.0
21	927.0
22	1334.0
23	1993.0
24	3217.0
25	5450.0
26	8811.0
27	10707.0
28	10615.0
29	9469.0
30	9477.0
31	10212.0
32	12228.0
33	16431.0
34	29957.0
35	39929.0
36	46084.0
37	64348.0
38	113448.0
39	152432.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.12608460314597	18.559949553765883	21.917503640402913	20.396462202685242
2	40.25747991171909	21.31699662659034	22.196889756389158	16.22863370530141
3	30.732108262575565	21.368937293945905	32.33133407326003	15.567620370218496
4	26.931873891704623	23.510350772640205	31.415173600290135	18.142601735365034
5	23.347785596215072	27.000399123022838	30.761632431388204	18.890182849373886
6	24.891972524298208	30.718257417947413	29.927301290497777	14.462468767256603
7	72.1390260304755	5.949666759613124	16.957443279880007	4.953863930031365
8	72.52721417597499	5.1040362454734165	17.035992148758073	5.332757429793531
9	65.56698250237378	7.86837323652322	19.630291797201764	6.934352463901236
10	34.814826964678524	24.441455577972054	24.87411222464612	15.869605232703302
11	27.782060604735165	22.143308857432892	30.85804159991835	19.21658893791359
12	25.141652223516182	19.615347464839815	34.90923140569671	20.3337689059473
13	23.136377967680147	20.068780378456104	36.79896045766107	19.99588119620268
14	19.960342844854136	23.283816563787695	35.24274516450612	21.51309542685205
15	18.896561527821063	22.70007636189341	37.319096123039245	21.084265987246287
16	21.05164360318788	21.764415357670725	36.9642593534207	20.21968168572069
17	20.712844653665098	22.32974851604602	34.08182568712036	22.875581143168528
18	22.149869783835697	22.22203997426659	33.61946262367802	22.00862761821969
19	22.680393582684985	24.22603849441319	31.908883312101448	21.184684610800378
20	24.881402142871462	22.560292179922474	31.352115807640928	21.206189869565137
21	23.57031035004365	24.242805306331476	31.798987794854412	20.387896548770463
22	22.558834196277402	22.379137712022715	31.500647891482274	23.561380200217606
23	22.279630328246792	24.329555333213047	31.460006597375994	21.930807741164163
24	22.028128149472483	23.160252449868143	32.62311305022936	22.188506350430014
25	22.867926729031918	23.254110147019425	31.26390779711429	22.61405532683437
26	21.424887416325408	24.340490210551064	31.375808041873288	22.858814331250237
27	23.46952723057829	22.375675000865677	31.555140030216712	22.59965773833932
28	22.233703843427136	23.82509299201936	31.56279444435332	22.378408720200184
29	22.931531265548028	23.48793427409728	30.505209557811785	23.075324902542906
30	24.544425672905014	21.72723677472148	30.61310034754685	23.115237204826656
31	25.485736363752338	22.372030041753007	28.961751621551183	23.18048197294347
32	24.683298615097783	24.2347863962836	28.922568311089968	22.159346677528642
33	24.973072864555142	24.71993045418013	27.470781096513047	22.836215584751677
34	24.482461367989604	24.300213412356047	28.95737767061598	22.25994754903837
35	24.075866178971136	23.87484668390732	29.412633063788608	22.63665407333293
36	24.732870059030112	25.97525437258408	28.422115424920218	20.86976014346559
37	24.16935938021115	26.884489423239895	28.767110804934546	20.179040391614407
38	23.395534560591067	26.010245980065715	29.318228622770427	21.27599083657279
39	23.628265199935118	24.521097934583917	29.126139277532655	22.72449758794831
40	23.493766208677556	23.48939225774235	32.449248500554944	20.56759303302515
41	21.16008113678985	24.914753518752402	31.486979294809757	22.43818604964799
42	22.377132984510748	26.585967271912125	29.151653991321353	21.885245752255774
43	23.483560323162074	24.2613945978061	29.925478810941435	22.329566268090385
44	24.357439270424987	24.483190359812138	28.93897062709699	22.220399742665887
45	23.291470977924305	23.12453185056397	29.32114459006056	24.262852581451167
46	23.186496155479375	23.76713814212789	29.566814834254597	23.479550868138137
47	23.389338130099528	24.283993344304662	30.377271492957025	21.94939703263879
48	22.739259672354624	23.26777874369194	30.494274680473772	23.49868690347966
49	22.659799563698392	23.73451575806948	30.157844954374223	23.447839723857896
50	20.89746183272189	22.6929686916237	32.18498896488629	24.22458051076812
51	21.631556598013862	22.446387207651497	30.685635033889007	25.236421160445634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	533.5
2	856.0
3	10253.5
4	19651.0
5	12553.5
6	5456.0
7	5359.0
8	5262.0
9	5091.0
10	4920.0
11	4718.0
12	4516.0
13	4291.0
14	4066.0
15	3791.0
16	3516.0
17	3281.5
18	3047.0
19	2923.0
20	2799.0
21	2658.0
22	2517.0
23	2493.0
24	2469.0
25	2735.5
26	3390.0
27	3778.0
28	4413.5
29	5049.0
30	5673.0
31	6297.0
32	6870.0
33	7443.0
34	8351.0
35	9259.0
36	10114.0
37	10969.0
38	11870.5
39	12772.0
40	14612.5
41	16453.0
42	18305.5
43	20158.0
44	23828.5
45	27499.0
46	32084.0
47	36669.0
48	41985.5
49	47302.0
50	46365.0
51	45428.0
52	41749.5
53	38071.0
54	35062.0
55	32053.0
56	30435.0
57	28817.0
58	28250.0
59	27683.0
60	26537.0
61	25391.0
62	24265.5
63	23140.0
64	20615.0
65	18090.0
66	15349.5
67	12609.0
68	11309.5
69	10010.0
70	8704.5
71	7399.0
72	6541.0
73	5683.0
74	4944.5
75	3140.5
76	2075.0
77	1605.5
78	1136.0
79	816.5
80	497.0
81	382.5
82	268.0
83	217.0
84	166.0
85	97.5
86	29.0
87	19.5
88	10.0
89	7.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	548703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.71447467336724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28146951988134	27.59624646407845
2	8.066571361330764	6.245861453382989
3	4.029508363666244	4.680008984738348
4	2.9022530137433002	4.49436803185075
5	2.300380362953976	4.452900865034652
6	1.9286437901530804	4.479985870069707
7	1.6942378891650285	4.591407089551708
8	1.4319818042369168	4.435073863428228
9	1.264904771776866	4.407311137605918
>10	5.007523817838738	26.923064639896875
>50	0.05287160300351129	1.4412343304353856
>100	0.03493302341093996	2.6666307525285067
>500	0.00424861095538459	1.1702033245452694
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.7206788393162115E-4	2.415703192853252
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13218	2.4089534775643653	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	978	0.1782385006096194	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	757	0.13796170241460315	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	754	0.1374149585477025	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	720	0.13121852805616155	Illumina Paired End PCR Primer 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	695	0.12666232916532258	TruSeq Adapter, Index 22 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	679	0.12374636187518567	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	636	0.11590969978294269	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	636	0.11590969978294269	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8224795563355768E-4	0.0	0.0	0.1365037187695347	0.0
2	1.8224795563355768E-4	0.0	0.0	0.46965298166767816	0.0
3	1.8224795563355768E-4	0.0	0.0	0.7946010865623115	0.0
4	1.8224795563355768E-4	0.0	0.0	1.1807845045498202	0.0
5	1.8224795563355768E-4	0.0	0.0	2.2469350450061327	0.0
6	1.8224795563355768E-4	0.0	0.0	3.656440733876068	0.0
7	1.8224795563355768E-4	0.0	0.0	4.656253018481765	0.0
8	1.8224795563355768E-4	0.0	0.0	6.153602221967075	0.0
9	1.8224795563355768E-4	0.0	0.0	7.085071523210188	0.0
10	1.8224795563355768E-4	0.0	0.0	8.346045128238774	0.0
11	1.8224795563355768E-4	0.0	0.0	9.57275611760825	0.0
12	1.8224795563355768E-4	0.0	0.0	10.406905010543044	0.0
13	1.8224795563355768E-4	0.0	0.0	10.865440866917075	0.0
14	1.8224795563355768E-4	0.0	0.0	11.06390889060202	0.0
15	1.8224795563355768E-4	0.0	0.0	11.31085487048549	0.0
16	1.8224795563355768E-4	0.0	0.0	11.699954255763137	0.0
17	1.8224795563355768E-4	0.0	0.0	12.13461562994917	0.0
18	1.8224795563355768E-4	0.0	0.0	12.6724293470238	0.0
19	1.8224795563355768E-4	0.0	0.0	12.970769250395934	0.0
20	1.8224795563355768E-4	0.0	0.0	13.285693717730721	0.0
21	1.8224795563355768E-4	0.0	0.0	13.656568307445012	0.0
22	1.8224795563355768E-4	0.0	0.0	14.024891425780432	0.0
23	1.8224795563355768E-4	0.0	0.0	14.390480824781347	0.0
24	1.8224795563355768E-4	0.0	0.0	14.695199406600656	0.0
25	1.8224795563355768E-4	0.0	0.0	14.97039381960733	0.0
26	1.8224795563355768E-4	0.0	0.0	15.235929090965422	0.0
27	1.8224795563355768E-4	0.0	0.0	15.510576760105193	0.0
28	3.6449591126711537E-4	0.0	0.0	15.814384102146334	0.0
29	3.6449591126711537E-4	0.0	0.0	16.10525183933749	0.0
30	5.46743866900673E-4	0.0	0.0	16.47138798220531	0.0
31	5.46743866900673E-4	0.0	0.0	16.781574002693624	0.0
32	5.46743866900673E-4	0.0	0.0	17.100325677096716	0.0
33	5.46743866900673E-4	0.0	0.0	17.406502242561093	0.0
34	5.46743866900673E-4	0.0	0.0	17.703748658199427	0.0
35	5.46743866900673E-4	0.0	0.0	18.025963043759557	0.0
36	5.46743866900673E-4	0.0	0.0	18.35601409141193	0.0
37	5.46743866900673E-4	0.0	0.0	18.67458351785939	0.0
38	5.46743866900673E-4	0.0	0.0	18.980942331279397	0.0
39	5.46743866900673E-4	0.0	0.0	19.303156716839528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	20	7.0300605E-4	45.0	1
TACGGGT	20	7.0300605E-4	45.0	4
TACGAAT	50	2.1827873E-11	45.0	12
CGAATAT	50	2.1827873E-11	45.0	14
TACTAGA	20	7.0300605E-4	45.0	30
CGCGTAA	25	3.8881313E-5	45.0	31
TCGCGTA	25	3.8881313E-5	45.0	30
GCGTAAG	40	6.8030204E-9	45.0	1
CGTTTTT	5805	0.0	43.720932	1
ACGGTCT	55	6.002665E-11	40.909092	30
CGGTCTA	50	1.0786607E-9	40.5	31
TCGACGG	40	3.4538243E-7	39.375	2
GTGCAAG	110	0.0	38.863636	1
CGCATCG	35	6.2423223E-6	38.571426	21
AATACGA	35	6.2423223E-6	38.571426	27
GTTTTTT	6795	0.0	38.443707	2
ACGTTAG	30	1.1391442E-4	37.500004	1
CGTTCTG	120	0.0	37.500004	1
CGTTCAT	30	1.1391442E-4	37.500004	17
CGTAAGG	60	1.546141E-10	37.500004	2
>>END_MODULE