FastQCFastQC Report
Sat 18 Jun 2016
SRR3551588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703983
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88921.2630986827806923No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC14530.20639702947372307TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC13590.193044434311624TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG11350.16122548413811127No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC10730.15241845328651402TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG10500.14915132893834085No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC9980.1417647869337754No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT9890.14048634697144674No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC9010.12798604511756675No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC8690.12344048080706493No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8650.12287228526825222No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT7680.10909354345204358Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA600.045.00000431
GGCGTAT207.0314226E-445.036
TAACGTC1250.043.232
CGTTTTT37050.041.9635621
CTACGAA1200.041.25000411
TACGAAT1200.041.25000412
GCTACGA1200.041.25000410
TAACGCC451.9264917E-840.012
ATAACGC451.9264917E-840.011
CGAATAT1250.039.60000214
AAGCTAC1250.039.6000028
CCCGATG403.4556797E-739.37515
ACATACG700.038.57142617
CGAAAGG659.094947E-1238.0769272
TACGAAA659.094947E-1238.07692720
TACTGAC1250.037.80000320
GACCTAT1250.037.80000333
TATACGG552.746674E-936.818182
TACGGGC800.036.56254
GGTACGT250.002106555336.01
Produced by FastQC (version 0.11.5)