##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703983
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08856890010128	33.0	31.0	34.0	30.0	34.0
2	32.200548876890494	33.0	31.0	34.0	30.0	34.0
3	32.13406573738286	33.0	31.0	34.0	30.0	34.0
4	35.7792816019705	37.0	35.0	37.0	35.0	37.0
5	35.81998428939335	37.0	35.0	37.0	35.0	37.0
6	35.712214641546744	37.0	35.0	37.0	35.0	37.0
7	36.00192476238773	37.0	35.0	37.0	35.0	37.0
8	35.815894702002744	37.0	35.0	37.0	35.0	37.0
9	37.65024865657267	39.0	37.0	39.0	35.0	39.0
10	37.333438449508016	39.0	37.0	39.0	34.0	39.0
11	37.404435902571514	39.0	37.0	39.0	35.0	39.0
12	37.34052810934355	39.0	37.0	39.0	35.0	39.0
13	37.41891068392277	39.0	37.0	39.0	35.0	39.0
14	38.53750729776145	40.0	38.0	41.0	34.0	41.0
15	38.60657146550414	40.0	38.0	41.0	35.0	41.0
16	38.36323462356335	40.0	38.0	41.0	34.0	41.0
17	38.39428650975947	40.0	38.0	41.0	34.0	41.0
18	38.13781582793903	40.0	37.0	41.0	34.0	41.0
19	37.926557885630764	40.0	37.0	41.0	34.0	41.0
20	38.166099181372275	40.0	37.0	41.0	34.0	41.0
21	38.026571664372575	40.0	36.0	41.0	34.0	41.0
22	38.179122223121865	40.0	37.0	41.0	34.0	41.0
23	38.23595598189161	40.0	37.0	41.0	34.0	41.0
24	38.09162863307779	40.0	36.0	41.0	34.0	41.0
25	37.709450370250416	40.0	36.0	41.0	33.0	41.0
26	37.886349528326676	40.0	36.0	41.0	34.0	41.0
27	37.982390199763344	40.0	36.0	41.0	34.0	41.0
28	37.86726241968911	40.0	36.0	41.0	34.0	41.0
29	37.887399269584634	40.0	36.0	41.0	34.0	41.0
30	37.62233747121734	40.0	36.0	41.0	33.0	41.0
31	37.50173512712665	40.0	35.0	41.0	33.0	41.0
32	37.47548307274465	40.0	36.0	41.0	33.0	41.0
33	37.366277310673695	40.0	35.0	41.0	33.0	41.0
34	37.19329159937101	40.0	35.0	41.0	33.0	41.0
35	37.09521394692769	40.0	35.0	41.0	32.0	41.0
36	36.9165860539246	40.0	35.0	41.0	32.0	41.0
37	36.92571269476678	40.0	35.0	41.0	32.0	41.0
38	36.7550651081063	39.0	35.0	41.0	31.0	41.0
39	36.67690697076492	39.0	35.0	41.0	31.0	41.0
40	36.56879214412848	39.0	35.0	41.0	31.0	41.0
41	36.383060386401375	39.0	35.0	41.0	31.0	41.0
42	36.4278427177929	39.0	35.0	41.0	31.0	41.0
43	36.39902668104202	39.0	35.0	41.0	31.0	41.0
44	36.40010341158806	39.0	35.0	41.0	31.0	41.0
45	36.3621820413277	39.0	35.0	41.0	31.0	41.0
46	36.271864519455725	39.0	35.0	41.0	31.0	41.0
47	36.22262611455106	39.0	35.0	41.0	31.0	41.0
48	36.14573647375008	39.0	35.0	41.0	31.0	41.0
49	36.10377807418645	38.0	35.0	41.0	31.0	41.0
50	35.98568431339962	38.0	35.0	41.0	30.0	41.0
51	35.13320350065271	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	24.0
10	37.0
11	32.0
12	29.0
13	32.0
14	39.0
15	56.0
16	103.0
17	122.0
18	245.0
19	371.0
20	576.0
21	940.0
22	1350.0
23	2058.0
24	3092.0
25	4955.0
26	7198.0
27	8418.0
28	8860.0
29	8695.0
30	9271.0
31	10858.0
32	14205.0
33	19966.0
34	38725.0
35	51880.0
36	55249.0
37	82331.0
38	146442.0
39	227706.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.898548686544984	20.44466982867484	23.66932156032177	15.987459924458403
2	36.483977596049904	23.982539351092285	23.21007751607638	16.323405536781426
3	31.48058973014973	23.176980125940542	28.587480095400032	16.754950048509695
4	28.43321500661238	24.26223360507285	28.58875853536236	18.715792852952415
5	25.17603408036842	28.16232778348341	26.316970722304372	20.3446674138438
6	25.675051812330697	33.4881666176598	26.11213623056239	14.724645339447118
7	75.89728729244882	6.130119619365808	12.91309591282744	5.059497175357928
8	76.12982131670793	6.212650021378357	12.254273185574084	5.403255476339627
9	69.40892038586159	8.331025038956906	14.476770035640065	7.783284539541438
10	37.78898069981803	24.70372153873034	21.08630464087911	16.420993120572515
11	29.153828998711617	23.164763921856068	27.11855257868443	20.56285450074789
12	26.682178404876254	20.33784906737805	31.639542432132593	21.340430095613105
13	24.37487837064247	21.15363581222842	33.433335748164374	21.038150068964732
14	20.7909850095812	26.0546064322576	31.375331506584676	21.77907705157653
15	19.72362968992149	23.895037238115123	34.94544612582974	21.435886946133643
16	21.672540388049143	22.381506371602725	33.98945713177733	21.956496108570803
17	22.210053367765983	22.659353990082145	30.651876536791374	24.478716105360498
18	22.326817550991997	23.441049002603755	30.772902186558483	23.459231259845765
19	24.402862000928998	24.37956598383768	29.019166656013002	22.19840535922032
20	25.63201100026563	24.08864986796556	28.41162357613749	21.867715555631314
21	24.53454131704885	24.85287286766868	28.953398022395426	21.659187792887042
22	22.82057947422026	23.41221307900901	28.650691849092947	25.116515597677786
23	23.18578715679214	24.8447760812406	28.502676911232232	23.46675985073503
24	23.43053738513572	24.099019436548893	29.18792073104038	23.282522447275007
25	23.65824174731492	24.614656888021443	28.36247466203019	23.364626702633444
26	21.8135949305594	25.439392712608118	28.99473424784405	23.752278108988428
27	23.70284509711172	24.086519134695013	29.716342582136217	22.49429318605705
28	22.052521154630153	24.673749224057968	30.59676156952654	22.67696805178534
29	22.993310918019326	23.95739669849982	29.923733953802863	23.12555842967799
30	23.003112291063847	23.254822914757884	30.35144314564414	23.390621648534125
31	23.88097439852951	23.677276297865145	28.109059451719716	24.33268985188563
32	23.448009397954213	24.374026077334253	28.787058778408003	23.390905746303535
33	22.851119984431442	23.91591842416649	28.09243973220944	25.14052185919262
34	22.36801172755592	23.17825856590287	29.463353518479853	24.990376188061358
35	23.302267242248746	22.323124279989713	29.65540361059855	24.719204867162986
36	23.28976694039487	24.22643728612765	28.1040877407551	24.37970803272238
37	22.810920150060443	24.469767025624197	29.35823734379949	23.361075480515865
38	22.85765423312779	24.93199409644835	27.487595581143292	24.722756089280566
39	22.9251274533618	23.372723489061524	28.443300477426302	25.25884858015037
40	23.289624891510165	23.027118552578685	30.853443904185184	22.829812651725963
41	21.73632033728087	23.704123537074047	29.791202344374796	24.768353781270285
42	22.018713520070797	25.229018314362705	28.467732885595247	24.28453527997125
43	22.675263465168904	23.22996435993483	29.57145271973897	24.523319455157296
44	23.274425660846923	23.46349272638686	28.71418770055527	24.54789391221095
45	22.331505164187206	23.202264827417707	28.84984438544681	25.61638562294828
46	23.329540628111758	23.96449914273498	28.61248069910779	24.09347953004547
47	22.710775686344697	23.88182669183773	29.935239913463818	23.472157708353752
48	22.222979816273973	23.467896241812657	29.555543244652217	24.753580697261153
49	21.99967896952057	22.79785165266775	30.10669291730056	25.09577646051112
50	20.961017524570906	22.51204929664495	31.10501247899452	25.421920699789624
51	21.573958462065136	22.986634620438277	29.09146953832692	26.347937379169668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	373.0
1	835.5
2	1298.0
3	7401.5
4	13505.0
5	8704.0
6	3903.0
7	3797.5
8	3692.0
9	3574.0
10	3456.0
11	3453.5
12	3451.0
13	3274.5
14	3098.0
15	2941.0
16	2784.0
17	2659.5
18	2535.0
19	2477.5
20	2420.0
21	2376.5
22	2333.0
23	2447.5
24	2562.0
25	3286.5
26	4404.5
27	4798.0
28	5832.5
29	6867.0
30	8048.5
31	9230.0
32	10419.5
33	11609.0
34	12571.5
35	13534.0
36	14151.5
37	14769.0
38	16274.0
39	17779.0
40	21230.0
41	24681.0
42	27701.5
43	30722.0
44	34591.5
45	38461.0
46	46785.0
47	55109.0
48	58794.5
49	62480.0
50	61694.0
51	60908.0
52	53896.5
53	46885.0
54	42962.5
55	39040.0
56	38312.5
57	37585.0
58	36715.5
59	35846.0
60	35133.5
61	34421.0
62	32117.0
63	29813.0
64	26870.5
65	23928.0
66	21083.0
67	18238.0
68	15359.0
69	12480.0
70	10926.5
71	9373.0
72	8086.0
73	6799.0
74	5706.5
75	3446.0
76	2278.0
77	1676.5
78	1075.0
79	868.0
80	661.0
81	495.0
82	329.0
83	237.0
84	145.0
85	92.0
86	39.0
87	36.5
88	34.0
89	31.0
90	28.0
91	15.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.55631387363843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85598161996148	22.99066223571537
2	8.261292517130842	5.213918793450427
3	3.0719576965276754	2.9081898383449993
4	1.8656353464763342	2.354902982686456
5	1.4066876007012843	2.2194937724942547
6	1.1850155259325459	2.243683312887729
7	0.9778979184368255	2.1601197555399096
8	0.9278546190088505	2.342373726923879
9	0.8315118344530716	2.3615503593881404
>10	8.472255715857795	46.9552983932314
>50	0.0864362725037484	1.8530612168450613
>100	0.04887496035455342	2.9055474344600816
>500	0.005883097079714764	1.354522629305705
>1k	0.00226272964604414	0.8668373600301139
>5k	4.52545929208828E-4	1.269838188696503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8892	1.2630986827806923	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	1453	0.20639702947372307	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1359	0.193044434311624	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG	1135	0.16122548413811127	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1073	0.15241845328651402	TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	1050	0.14915132893834085	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC	998	0.1417647869337754	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	989	0.14048634697144674	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	901	0.12798604511756675	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	869	0.12344048080706493	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	865	0.12287228526825222	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	768	0.10909354345204358	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11221861891551359	0.0
2	0.0	0.0	0.0	0.5622294856551934	0.0
3	0.0	0.0	0.0	0.9187721862601795	0.0
4	0.0	0.0	0.0	1.3488962091414138	0.0
5	0.0	0.0	0.0	2.560573195659554	0.0
6	0.0	0.0	0.0	3.658469025530446	0.0
7	0.0	0.0	0.0	4.490449343236982	0.0
8	0.0	0.0	0.0	5.675421139430924	0.0
9	1.4204888470318176E-4	0.0	0.0	6.302851063164877	0.0
10	1.4204888470318176E-4	0.0	0.0	7.430151012169328	0.0
11	1.4204888470318176E-4	0.0	0.0	8.611287488476284	0.0
12	1.4204888470318176E-4	0.0	0.0	9.5897202063118	0.0
13	1.4204888470318176E-4	0.0	0.0	9.977087514897377	0.0
14	1.4204888470318176E-4	0.0	0.0	10.150103056465852	0.0
15	1.4204888470318176E-4	0.0	0.0	10.421132328479523	0.0
16	1.4204888470318176E-4	0.0	0.0	10.920860304865316	0.0
17	1.4204888470318176E-4	0.0	0.0	11.463202946662065	0.0
18	1.4204888470318176E-4	0.0	0.0	12.124866651609485	0.0
19	1.4204888470318176E-4	0.0	0.0	12.503995124882277	0.0
20	1.4204888470318176E-4	0.0	0.0	12.86792436749183	0.0
21	1.4204888470318176E-4	0.0	0.0	13.281996866401604	0.0
22	2.840977694063635E-4	0.0	0.0	13.717376698016855	0.0
23	2.840977694063635E-4	0.0	0.0	14.145085889858136	0.0
24	2.840977694063635E-4	0.0	0.0	14.502907030425451	0.0
25	2.840977694063635E-4	0.0	0.0	14.832318394052129	0.0
26	2.840977694063635E-4	0.0	0.0	15.130905149698217	0.0
27	2.840977694063635E-4	0.0	0.0	15.432190834153666	0.0
28	2.840977694063635E-4	0.0	0.0	15.741999451691305	0.0
29	2.840977694063635E-4	0.0	0.0	16.047830700457254	0.0
30	2.840977694063635E-4	0.0	0.0	16.40337905886932	0.0
31	2.840977694063635E-4	0.0	0.0	16.774552794598733	0.0
32	2.840977694063635E-4	0.0	0.0	17.124703295392077	0.0
33	2.840977694063635E-4	0.0	0.0	17.457807930021037	0.0
34	2.840977694063635E-4	0.0	0.0	17.755968539013015	0.0
35	2.840977694063635E-4	0.0	0.0	18.08694244037143	0.0
36	2.840977694063635E-4	0.0	0.0	18.409819555301763	0.0
37	2.840977694063635E-4	0.0	0.0	18.738662723389627	0.0
38	2.840977694063635E-4	0.0	0.0	19.04960773200489	0.0
39	2.840977694063635E-4	0.0	0.0	19.353166198615593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	60	0.0	45.000004	31
GGCGTAT	20	7.0314226E-4	45.0	36
TAACGTC	125	0.0	43.2	32
CGTTTTT	3705	0.0	41.963562	1
CTACGAA	120	0.0	41.250004	11
TACGAAT	120	0.0	41.250004	12
GCTACGA	120	0.0	41.250004	10
TAACGCC	45	1.9264917E-8	40.0	12
ATAACGC	45	1.9264917E-8	40.0	11
CGAATAT	125	0.0	39.600002	14
AAGCTAC	125	0.0	39.600002	8
CCCGATG	40	3.4556797E-7	39.375	15
ACATACG	70	0.0	38.571426	17
CGAAAGG	65	9.094947E-12	38.076927	2
TACGAAA	65	9.094947E-12	38.076927	20
TACTGAC	125	0.0	37.800003	20
GACCTAT	125	0.0	37.800003	33
TATACGG	55	2.746674E-9	36.81818	2
TACGGGC	80	0.0	36.5625	4
GGTACGT	25	0.0021065553	36.0	1
>>END_MODULE