Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551586_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 669055 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3074 | 0.4594540060234211 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 1586 | 0.2370507656321229 | TruSeq Adapter, Index 18 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT | 1061 | 0.15858188041341892 | RNA PCR Primer, Index 34 (96% over 26bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC | 807 | 0.12061788642189356 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 784 | 0.11718020192659796 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 781 | 0.11673180829677679 | RNA PCR Primer, Index 34 (96% over 25bp) |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 737 | 0.11015536839273303 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAGAACG | 25 | 3.889051E-5 | 45.000004 | 1 |
CGATTCG | 35 | 1.2109012E-7 | 45.000004 | 10 |
TTATACG | 35 | 1.2109012E-7 | 45.000004 | 1 |
CAATCGC | 25 | 3.889051E-5 | 45.000004 | 45 |
TCTAACG | 20 | 7.031171E-4 | 45.0 | 1 |
CCTATAC | 20 | 7.031171E-4 | 45.0 | 15 |
CGAATAT | 125 | 0.0 | 44.999996 | 14 |
AGTAACG | 30 | 2.1641372E-6 | 44.999996 | 1 |
CTACGAA | 130 | 0.0 | 43.269234 | 11 |
CTTATCG | 60 | 3.6379788E-12 | 41.249996 | 12 |
TATCGAC | 60 | 3.6379788E-12 | 41.249996 | 14 |
CGTAAGG | 55 | 6.002665E-11 | 40.909092 | 2 |
TTTGCGC | 55 | 6.002665E-11 | 40.909092 | 13 |
GCGTAAG | 45 | 1.9263098E-8 | 40.0 | 1 |
GATTTCG | 40 | 3.455334E-7 | 39.375 | 9 |
TACGAAT | 145 | 0.0 | 38.793102 | 12 |
GCTACGA | 145 | 0.0 | 38.793102 | 10 |
ATTAGCG | 35 | 6.244374E-6 | 38.57143 | 1 |
TATGACG | 35 | 6.244374E-6 | 38.57143 | 1 |
ATAACGC | 70 | 0.0 | 38.57143 | 11 |