FastQCFastQC Report
Sat 18 Jun 2016
SRR3551586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669055
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.4594540060234211No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC15860.2370507656321229TruSeq Adapter, Index 18 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT10610.15858188041341892RNA PCR Primer, Index 34 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC8070.12061788642189356No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7840.11718020192659796No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC7810.11673180829677679RNA PCR Primer, Index 34 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7370.11015536839273303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG253.889051E-545.0000041
CGATTCG351.2109012E-745.00000410
TTATACG351.2109012E-745.0000041
CAATCGC253.889051E-545.00000445
TCTAACG207.031171E-445.01
CCTATAC207.031171E-445.015
CGAATAT1250.044.99999614
AGTAACG302.1641372E-644.9999961
CTACGAA1300.043.26923411
CTTATCG603.6379788E-1241.24999612
TATCGAC603.6379788E-1241.24999614
CGTAAGG556.002665E-1140.9090922
TTTGCGC556.002665E-1140.90909213
GCGTAAG451.9263098E-840.01
GATTTCG403.455334E-739.3759
TACGAAT1450.038.79310212
GCTACGA1450.038.79310210
ATTAGCG356.244374E-638.571431
TATGACG356.244374E-638.571431
ATAACGC700.038.5714311