##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669055
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05807594293444	33.0	31.0	34.0	30.0	34.0
2	32.13176495205925	33.0	31.0	34.0	30.0	34.0
3	32.09754056094043	33.0	31.0	34.0	30.0	34.0
4	35.74160420294296	37.0	35.0	37.0	33.0	37.0
5	35.79813169320908	37.0	35.0	37.0	35.0	37.0
6	35.68363587447968	37.0	35.0	37.0	35.0	37.0
7	36.02002227021695	37.0	35.0	37.0	35.0	37.0
8	35.81488517386463	37.0	35.0	37.0	35.0	37.0
9	37.6661096621354	39.0	38.0	39.0	35.0	39.0
10	37.27648997466576	39.0	37.0	39.0	34.0	39.0
11	37.34710748742629	39.0	37.0	39.0	34.0	39.0
12	37.27410302590968	39.0	37.0	39.0	34.0	39.0
13	37.330518417768346	39.0	37.0	39.0	34.0	39.0
14	38.3788477778359	40.0	38.0	41.0	34.0	41.0
15	38.47116754227978	40.0	38.0	41.0	34.0	41.0
16	38.24049741800002	40.0	37.0	41.0	34.0	41.0
17	38.27169814140841	40.0	37.0	41.0	34.0	41.0
18	38.084395154359505	40.0	37.0	41.0	34.0	41.0
19	37.92829886930073	40.0	37.0	41.0	34.0	41.0
20	38.24130303188826	40.0	37.0	41.0	34.0	41.0
21	38.08368220848809	40.0	37.0	41.0	34.0	41.0
22	38.24250323217075	40.0	37.0	41.0	34.0	41.0
23	38.31136304190238	40.0	37.0	41.0	34.0	41.0
24	38.176867372637524	40.0	37.0	41.0	34.0	41.0
25	37.79428298121978	40.0	36.0	41.0	33.0	41.0
26	37.99248940670049	40.0	37.0	41.0	34.0	41.0
27	38.10322918145743	40.0	37.0	41.0	34.0	41.0
28	38.02089962708597	40.0	37.0	41.0	34.0	41.0
29	38.046050025782634	40.0	37.0	41.0	34.0	41.0
30	37.75266756843608	40.0	36.0	41.0	33.0	41.0
31	37.66476597589137	40.0	36.0	41.0	33.0	41.0
32	37.72053717556852	40.0	36.0	41.0	33.0	41.0
33	37.69276217949197	40.0	36.0	41.0	33.0	41.0
34	37.5862985853181	40.0	36.0	41.0	33.0	41.0
35	37.54197935894658	40.0	36.0	41.0	33.0	41.0
36	37.37687335121926	40.0	35.0	41.0	33.0	41.0
37	37.386132679675065	40.0	35.0	41.0	33.0	41.0
38	37.217868486148376	40.0	35.0	41.0	33.0	41.0
39	37.184534903707466	39.0	35.0	41.0	33.0	41.0
40	37.084514725994126	39.0	35.0	41.0	32.0	41.0
41	36.9233486036275	39.0	35.0	41.0	32.0	41.0
42	37.00483368332947	39.0	35.0	41.0	32.0	41.0
43	36.99179738586514	39.0	35.0	41.0	32.0	41.0
44	37.00046035079328	39.0	35.0	41.0	33.0	41.0
45	36.94639753084574	39.0	35.0	41.0	32.0	41.0
46	36.833868665505825	39.0	35.0	41.0	32.0	41.0
47	36.783905657980284	39.0	35.0	41.0	32.0	41.0
48	36.727783216626435	39.0	35.0	41.0	32.0	41.0
49	36.697882834744526	39.0	35.0	41.0	32.0	41.0
50	36.58475312194065	39.0	35.0	41.0	32.0	41.0
51	35.73502028981175	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	12.0
9	34.0
10	26.0
11	33.0
12	29.0
13	25.0
14	52.0
15	58.0
16	77.0
17	108.0
18	169.0
19	278.0
20	450.0
21	640.0
22	1014.0
23	1435.0
24	2103.0
25	3347.0
26	4501.0
27	5479.0
28	6018.0
29	6719.0
30	8000.0
31	10310.0
32	13537.0
33	19570.0
34	38415.0
35	51837.0
36	51027.0
37	79362.0
38	146555.0
39	217734.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49187286545949	21.276875593187405	24.680631637159873	11.550619904193228
2	34.16699673419973	24.295162580056946	24.47048448931702	17.067356196426303
3	33.162146609770495	22.80470215453139	26.878208816913407	17.154942418784707
4	29.333612333814113	25.88710943046536	25.681595683463993	19.09768255225654
5	25.254127089701147	30.176442893334627	24.293817399167484	20.275612617796742
6	27.05607162340914	34.106613058717144	24.08411864495445	14.753196672919266
7	80.09072497776715	6.094865145615831	9.098205678158	4.71620419845902
8	80.32658002705308	5.720456464715158	8.929460208801968	5.023503299429793
9	73.76837479728871	8.418291470805839	11.211484855505152	6.601848876400296
10	37.55132238754661	28.442205797729635	18.068170778187145	15.938301036536606
11	29.896495803782948	24.35748929460209	25.351727436458887	20.394287465156076
12	27.670071967177584	22.25601781617356	29.85643930618559	20.217470910463263
13	25.41898648093206	23.92673248088722	30.970249082661365	19.68403195551935
14	21.71914117673435	27.216596542885114	29.452137716630173	21.612124563750363
15	20.42731912921957	25.507170561463553	33.444335667471286	20.621174641845588
16	23.31228374348895	23.263109908751897	31.905149800838494	21.519456546920658
17	22.638497582411013	23.6367712669362	28.771625651104916	24.95310549954787
18	23.389257983274916	24.428186023570557	29.805023503299427	22.377532489855096
19	24.936066541614665	25.946745783231574	27.54586693171712	21.57132074343664
20	26.02775556568593	24.863277309040363	27.123031738795767	21.98593538647794
21	25.185223935251962	25.665304048247155	27.73090403628999	21.418567980210895
22	23.148321139517677	24.150779831254532	27.171308786273173	25.529590242954615
23	22.602177698395497	26.226543408239984	27.737779405280584	23.43349948808394
24	23.016792341436805	25.582500691273513	28.43308846058994	22.967618506699747
25	23.128442355262273	25.994723901622436	27.55348962342408	23.323344119691207
26	21.59508560581716	27.02617871475439	27.62089813244053	23.75783754698792
27	23.0549057999716	25.153836381164478	28.945901308562078	22.845356510301844
28	22.65000635224309	25.735253454499258	29.02437019378078	22.590369999476874
29	23.445905045175657	25.03232170748294	28.38555873582889	23.136214511512506
30	24.448513201455786	24.158850916591312	28.447735985830764	22.94489989612214
31	25.971257968328466	26.14598201941544	25.02963134570402	22.853128666552077
32	25.792946768202913	26.669705779046566	25.708648765796532	21.828698686953988
33	24.576305385954818	25.098982893783024	25.808640545246654	24.516071175015508
34	23.9086472711511	25.90086016844654	26.769249164866864	23.421243395535495
35	24.47840611011053	25.24934422431639	26.233717706317115	24.038531959255966
36	24.58213450314249	26.522333739378677	25.67726121170905	23.21827054576978
37	24.097122060219263	27.44677193952665	26.340584854757832	22.11552114549626
38	23.134420936993223	28.155981197360457	24.901241303031888	23.80835656261443
39	23.912383884732943	24.928443849907705	25.88142977782096	25.277742487538397
40	23.326482875099956	25.153686916621204	28.446540269484572	23.073289938794268
41	21.004850124429232	26.199191396820886	27.958389071152595	24.837569407597282
42	23.31392785346496	26.03941380006128	26.43310340704426	24.213554939429493
43	23.418851962843114	24.26242984508	27.866019983409434	24.45269820866745
44	24.004304578846284	24.567935371531487	27.354851245413307	24.07290880420892
45	23.81896854518687	24.72890868463729	27.091793649251557	24.36032912092429
46	23.983977400961056	25.647517767597584	27.031111044682426	23.337393786758938
47	22.615031649117036	26.28946798095822	27.905478622833694	23.19002174709105
48	22.155129249463794	25.051453169021983	28.43413471239285	24.359282869121373
49	23.201381052379848	23.845423769346315	28.54937187525689	24.403823303016942
50	21.601512581177932	23.681461165375044	29.272331871071884	25.444694382375143
51	21.873089656306284	23.849459312014705	27.38833130310662	26.889119728572393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	316.0
1	792.0
2	1268.0
3	3180.5
4	5093.0
5	3410.5
6	1728.0
7	1775.5
8	1823.0
9	1745.0
10	1667.0
11	1669.0
12	1671.0
13	1689.0
14	1707.0
15	1638.0
16	1569.0
17	1665.0
18	1761.0
19	1752.5
20	1744.0
21	1879.5
22	2015.0
23	2169.0
24	2323.0
25	3308.0
26	5384.5
27	6476.0
28	6932.0
29	7388.0
30	8462.0
31	9536.0
32	10767.0
33	11998.0
34	13533.0
35	15068.0
36	16675.0
37	18282.0
38	19594.0
39	20906.0
40	23111.5
41	25317.0
42	27936.0
43	30555.0
44	33748.0
45	36941.0
46	40436.5
47	43932.0
48	49160.0
49	54388.0
50	59600.5
51	64813.0
52	63266.5
53	61720.0
54	54543.5
55	47367.0
56	42313.5
57	37260.0
58	34369.5
59	31479.0
60	31241.0
61	31003.0
62	27945.5
63	24888.0
64	21724.0
65	18560.0
66	16058.5
67	13557.0
68	11465.5
69	9374.0
70	8268.0
71	7162.0
72	6219.0
73	5276.0
74	4151.0
75	2410.0
76	1794.0
77	1472.5
78	1151.0
79	811.0
80	471.0
81	385.5
82	300.0
83	172.5
84	45.0
85	32.5
86	20.0
87	14.5
88	9.0
89	11.5
90	14.0
91	7.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.33050254571752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15990922843827	21.458169038691715
2	9.44448465480133	5.5402296242128095
3	3.3371074108299106	2.9363711222603848
4	1.7155410478856734	2.0127072428917456
5	1.129283962634588	1.6561233070445875
6	0.8923037233329347	1.5703029977261918
7	0.7426938164807929	1.5248508012484987
8	0.6342995805815658	1.4883460370396138
9	0.5319223386314286	1.4041394556617819
>10	8.19160366043559	52.689375461002506
>50	0.15936085642653525	3.037736222224494
>100	0.05534074756927007	2.928472703838389
>500	0.004611728964105839	0.8933457403959653
>1k	0.00153724298803528	0.8598302457613253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3074	0.4594540060234211	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1586	0.2370507656321229	TruSeq Adapter, Index 18 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1061	0.15858188041341892	RNA PCR Primer, Index 34 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC	807	0.12061788642189356	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	784	0.11718020192659796	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	781	0.11673180829677679	RNA PCR Primer, Index 34 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	737	0.11015536839273303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1651583203174627	0.0
2	0.0	0.0	0.0	0.6977004880017338	0.0
3	0.0	0.0	0.0	0.9691280985868127	0.0
4	0.0	0.0	0.0	1.358632698358132	0.0
5	0.0	0.0	0.0	2.4068275403367436	0.0
6	0.0	0.0	0.0	3.1211185926418605	0.0
7	0.0	0.0	0.0	3.7940079664601565	0.0
8	0.0	0.0	0.0	4.938159045220497	0.0
9	0.0	0.0	0.0	5.448729925043532	0.0
10	0.0	0.0	0.0	6.459259702117166	0.0
11	0.0	0.0	0.0	7.834781893865228	0.0
12	0.0	0.0	0.0	8.81317679413501	0.0
13	0.0	0.0	0.0	9.226894649916673	0.0
14	0.0	0.0	0.0	9.388316356652293	0.0
15	0.0	0.0	0.0	9.702042432983836	0.0
16	0.0	0.0	0.0	10.338910851873164	0.0
17	0.0	0.0	0.0	11.091464827256354	0.0
18	0.0	0.0	0.0	11.92742001778628	0.0
19	0.0	0.0	0.0	12.37192756948233	0.0
20	0.0	0.0	0.0	12.836463369977057	0.0
21	0.0	0.0	0.0	13.391724148238934	0.0
22	1.4946454327372188E-4	0.0	0.0	13.945639745611347	0.0
23	1.4946454327372188E-4	0.0	0.0	14.515398584570775	0.0
24	1.4946454327372188E-4	0.0	0.0	14.966781505257416	0.0
25	1.4946454327372188E-4	0.0	0.0	15.379154180149614	0.0
26	1.4946454327372188E-4	0.0	0.0	15.729050675953397	0.0
27	1.4946454327372188E-4	0.0	0.0	16.09583666514711	0.0
28	1.4946454327372188E-4	0.0	0.0	16.4860885876348	0.0
29	1.4946454327372188E-4	0.0	0.0	16.889044996300754	0.0
30	1.4946454327372188E-4	0.0	0.0	17.331758973477516	0.0
31	1.4946454327372188E-4	0.0	0.0	17.742935932023524	0.0
32	1.4946454327372188E-4	0.0	0.0	18.135130893573773	0.0
33	5.978581730948875E-4	0.0	0.0	18.54137552219175	0.0
34	5.978581730948875E-4	0.0	0.0	18.91488741583278	0.0
35	5.978581730948875E-4	0.0	0.0	19.361786400221206	0.0
36	5.978581730948875E-4	0.0	0.0	19.78237962499346	0.0
37	5.978581730948875E-4	0.0	0.0	20.2013287397897	0.0
38	5.978581730948875E-4	0.0	0.0	20.59561620494578	0.0
39	5.978581730948875E-4	0.0	0.0	20.976152932120677	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	25	3.889051E-5	45.000004	1
CGATTCG	35	1.2109012E-7	45.000004	10
TTATACG	35	1.2109012E-7	45.000004	1
CAATCGC	25	3.889051E-5	45.000004	45
TCTAACG	20	7.031171E-4	45.0	1
CCTATAC	20	7.031171E-4	45.0	15
CGAATAT	125	0.0	44.999996	14
AGTAACG	30	2.1641372E-6	44.999996	1
CTACGAA	130	0.0	43.269234	11
CTTATCG	60	3.6379788E-12	41.249996	12
TATCGAC	60	3.6379788E-12	41.249996	14
CGTAAGG	55	6.002665E-11	40.909092	2
TTTGCGC	55	6.002665E-11	40.909092	13
GCGTAAG	45	1.9263098E-8	40.0	1
GATTTCG	40	3.455334E-7	39.375	9
TACGAAT	145	0.0	38.793102	12
GCTACGA	145	0.0	38.793102	10
ATTAGCG	35	6.244374E-6	38.57143	1
TATGACG	35	6.244374E-6	38.57143	1
ATAACGC	70	0.0	38.57143	11
>>END_MODULE