Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551585_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 374582 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4186 | 1.1175123203998056 | No Hit |
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC | 819 | 0.21864371486083156 | Illumina PCR Primer Index 7 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT | 528 | 0.14095712020331996 | TruSeq Adapter, Index 23 (96% over 26bp) |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 520 | 0.13882140626084544 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC | 489 | 0.13054551473375656 | No Hit |
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC | 457 | 0.12200265896385838 | Illumina PCR Primer Index 7 (95% over 23bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 436 | 0.1163964098648627 | No Hit |
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 391 | 0.10438301893844339 | No Hit |
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 380 | 0.10144641226754088 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGACG | 45 | 3.8380676E-10 | 45.000004 | 26 |
TCACGAC | 50 | 2.1827873E-11 | 45.0 | 25 |
TCGTTTA | 40 | 6.7939254E-9 | 45.0 | 38 |
AAATACG | 20 | 7.02719E-4 | 45.0 | 1 |
CGCATCG | 40 | 6.7939254E-9 | 45.0 | 21 |
TATAGCG | 25 | 3.8857543E-5 | 45.0 | 1 |
CACAACG | 20 | 7.02719E-4 | 45.0 | 12 |
GCTCGTC | 20 | 7.02719E-4 | 45.0 | 27 |
CGTCCAT | 20 | 7.02719E-4 | 45.0 | 30 |
CGTAAGG | 80 | 0.0 | 42.1875 | 2 |
CGTTTTT | 1630 | 0.0 | 41.68712 | 1 |
CGTTCAT | 55 | 6.002665E-11 | 40.909092 | 17 |
GCGTAAG | 55 | 6.002665E-11 | 40.909092 | 1 |
TAACGGG | 105 | 0.0 | 40.714283 | 3 |
CGGTCTA | 50 | 1.0768417E-9 | 40.5 | 31 |
TAACGCC | 45 | 1.922308E-8 | 40.000004 | 12 |
ATGGTCG | 45 | 1.922308E-8 | 40.000004 | 44 |
ATAACGC | 45 | 1.922308E-8 | 40.000004 | 11 |
AGTCGGC | 45 | 1.922308E-8 | 40.000004 | 30 |
CCAGTCG | 40 | 3.4498953E-7 | 39.375 | 28 |