FastQCFastQC Report
Sat 18 Jun 2016
SRR3551585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374582
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41861.1175123203998056No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC8190.21864371486083156Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT5280.14095712020331996TruSeq Adapter, Index 23 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5200.13882140626084544No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC4890.13054551473375656No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC4570.12200265896385838Illumina PCR Primer Index 7 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4360.1163964098648627No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3910.10438301893844339No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3800.10144641226754088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG453.8380676E-1045.00000426
TCACGAC502.1827873E-1145.025
TCGTTTA406.7939254E-945.038
AAATACG207.02719E-445.01
CGCATCG406.7939254E-945.021
TATAGCG253.8857543E-545.01
CACAACG207.02719E-445.012
GCTCGTC207.02719E-445.027
CGTCCAT207.02719E-445.030
CGTAAGG800.042.18752
CGTTTTT16300.041.687121
CGTTCAT556.002665E-1140.90909217
GCGTAAG556.002665E-1140.9090921
TAACGGG1050.040.7142833
CGGTCTA501.0768417E-940.531
TAACGCC451.922308E-840.00000412
ATGGTCG451.922308E-840.00000444
ATAACGC451.922308E-840.00000411
AGTCGGC451.922308E-840.00000430
CCAGTCG403.4498953E-739.37528