##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374582
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.113214196090574	33.0	31.0	34.0	30.0	34.0
2	32.2228884463215	33.0	31.0	34.0	30.0	34.0
3	32.16230625070078	33.0	31.0	34.0	30.0	34.0
4	35.78969090879968	37.0	35.0	37.0	35.0	37.0
5	35.833457026765835	37.0	35.0	37.0	35.0	37.0
6	35.736193944183114	37.0	35.0	37.0	35.0	37.0
7	36.02455804069603	37.0	35.0	37.0	35.0	37.0
8	35.83933290975007	37.0	35.0	37.0	35.0	37.0
9	37.69345296890934	39.0	38.0	39.0	35.0	39.0
10	37.34494449813392	39.0	37.0	39.0	34.0	39.0
11	37.42913434174627	39.0	37.0	39.0	35.0	39.0
12	37.37097885109269	39.0	37.0	39.0	35.0	39.0
13	37.48329070804257	39.0	37.0	39.0	35.0	39.0
14	38.59856052880277	40.0	38.0	41.0	35.0	41.0
15	38.68162645295289	40.0	38.0	41.0	35.0	41.0
16	38.40990223769428	40.0	38.0	41.0	34.0	41.0
17	38.453011623623134	40.0	38.0	41.0	34.0	41.0
18	38.2309187307452	40.0	37.0	41.0	34.0	41.0
19	38.01464832800295	40.0	37.0	41.0	34.0	41.0
20	38.301143674816196	40.0	37.0	41.0	34.0	41.0
21	38.16171625972417	40.0	37.0	41.0	34.0	41.0
22	38.31519133327282	40.0	37.0	41.0	34.0	41.0
23	38.36513500381759	40.0	37.0	41.0	34.0	41.0
24	38.23546246215782	40.0	37.0	41.0	34.0	41.0
25	37.86423533431932	40.0	36.0	41.0	33.0	41.0
26	38.03398988739448	40.0	37.0	41.0	34.0	41.0
27	38.13143717530474	40.0	37.0	41.0	34.0	41.0
28	38.04357123406891	40.0	37.0	41.0	34.0	41.0
29	38.056385517723754	40.0	37.0	41.0	34.0	41.0
30	37.76145944012259	40.0	36.0	41.0	33.0	41.0
31	37.65009530623468	40.0	36.0	41.0	33.0	41.0
32	37.65334960035453	40.0	36.0	41.0	33.0	41.0
33	37.58079939772867	40.0	36.0	41.0	33.0	41.0
34	37.45441051625545	40.0	36.0	41.0	33.0	41.0
35	37.367604423063575	40.0	36.0	41.0	33.0	41.0
36	37.22830782045053	40.0	36.0	41.0	33.0	41.0
37	37.259523949362226	40.0	36.0	41.0	33.0	41.0
38	37.067934390867684	40.0	35.0	41.0	32.0	41.0
39	37.016725309812	40.0	35.0	41.0	32.0	41.0
40	36.953844552060694	40.0	35.0	41.0	32.0	41.0
41	36.80150140690156	39.0	35.0	41.0	31.0	41.0
42	36.85894677266927	39.0	35.0	41.0	32.0	41.0
43	36.836914213710216	39.0	35.0	41.0	32.0	41.0
44	36.83310463396533	39.0	35.0	41.0	32.0	41.0
45	36.79174119418445	39.0	35.0	41.0	32.0	41.0
46	36.705869475842405	39.0	35.0	41.0	32.0	41.0
47	36.675155239707195	39.0	35.0	41.0	32.0	41.0
48	36.606956554239126	39.0	35.0	41.0	32.0	41.0
49	36.569685676300516	39.0	35.0	41.0	32.0	41.0
50	36.46665349643069	39.0	35.0	41.0	31.0	41.0
51	35.63115686284979	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	17.0
10	21.0
11	22.0
12	12.0
13	22.0
14	24.0
15	28.0
16	51.0
17	63.0
18	103.0
19	159.0
20	265.0
21	423.0
22	605.0
23	909.0
24	1395.0
25	2127.0
26	3154.0
27	3891.0
28	3968.0
29	4175.0
30	4601.0
31	5418.0
32	7130.0
33	10140.0
34	19524.0
35	26225.0
36	27687.0
37	42919.0
38	81112.0
39	128306.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99636394701294	20.83896182945256	24.14531397664597	14.019360246888532
2	35.99318707252351	22.894586499084312	24.308963057488082	16.8032633709041
3	32.34485373029137	22.8398588293084	28.60495165277563	16.210335787624604
4	29.204820306368166	24.59568265426529	27.402544703162455	18.79695233620409
5	24.859977254646513	29.235521194291238	25.92516458345569	19.97933696760656
6	26.417980575681693	33.20741519880827	25.42727627061631	14.947327954893721
7	77.48770629661863	6.182624899220998	11.435146376494332	4.894522427666039
8	77.84490445349749	5.703691047621081	11.192475879780662	5.258928619100757
9	71.52452600498688	8.10049601956314	13.44458623212007	6.930391743329898
10	36.37601379671207	27.314980431521	20.243631567987784	16.065374203779147
11	28.103058876294106	23.78117474945406	27.72477054423331	20.390995830018525
12	25.504695901031017	21.24287872882306	32.459648354699375	20.79277701544655
13	23.911987228430622	22.164706259243637	33.770976715378744	20.152329796946997
14	20.372842261507493	26.065053846687775	31.895018981157662	21.66708491064707
15	19.921672691159745	24.48569338622785	34.59616318990235	20.99647073271006
16	21.67829740884506	23.27073911720264	33.166836633901255	21.884126840051042
17	21.452178695185566	23.42477748530362	30.355169228633518	24.767874590877298
18	22.454629426934556	24.2980175235329	30.962779845267523	22.28457320426502
19	23.822554207089503	25.700647655253057	28.68317217591876	21.793625961738687
20	24.126359515406506	25.160845956292615	28.946665883571555	21.766128644729324
21	23.767826537313592	25.414995915447086	29.666134517942666	21.151043029296655
22	22.291781238820874	24.201109503393113	28.472270424099396	25.034838833686617
23	21.885728625507898	25.435018233657786	29.161572099033055	23.51768104180126
24	22.442616036008136	24.858642433432465	29.709649689520585	22.98909184103881
25	23.02059362169031	25.602938742384847	28.29927759475896	23.07719004116589
26	20.928127886550875	26.49753592003887	28.413004362195725	24.16133183121453
27	22.408444612928545	25.783673534766756	29.471250620691862	22.336631231612838
28	21.8742491630671	25.744162826830973	29.723531830146673	22.658056179955256
29	22.161235724087117	26.417980575681693	28.390045437314125	23.030738262917065
30	23.04061593990101	26.0658547394162	28.618299864916093	22.275229455766695
31	23.801464031907564	26.58750286986561	26.78639123075855	22.82464186746827
32	22.904197211825448	27.319785787891572	27.557650928234672	22.21836607204831
33	22.521370487636887	26.264209171823527	27.149462600979223	24.064957739560363
34	21.89240273157813	25.717466402550045	28.535273985402394	23.85485688046943
35	21.21164391241437	25.043648653699325	29.740884505929277	24.00382292795703
36	22.316875877644947	26.96739298738327	27.7648151806547	22.95091595431708
37	22.0691330603179	26.4382698581352	29.091627467417013	22.400969614129885
38	21.512245649817665	26.061583311531255	28.686642711075276	23.739528327575805
39	21.79149024779621	23.45093998109893	29.303330111964804	25.454239659140054
40	21.39344656176752	23.74113011303266	32.33017069693685	22.53525262826297
41	19.946233401498205	25.169922740548127	30.300441558857607	24.58340229909606
42	20.681185961952256	26.77491176831775	29.22724530276415	23.316656966965844
43	22.259478565440947	24.754259414494022	28.80384001366857	24.182422006396465
44	22.302192844290435	24.631722827044545	29.14128281657955	23.924801512085473
45	21.88492773277947	24.13944076330416	29.08228371891869	24.893347784997676
46	22.831315973538505	24.784159409688666	29.057723008580233	23.3268016081926
47	21.479409047952117	25.03003347731605	30.50573706157797	22.984820413153862
48	20.791442194232506	24.49396927775494	30.18190943505027	24.53267909296229
49	21.33738407077756	23.860463129568426	30.47370135244085	24.32845144721316
50	20.281540490466707	23.280349829943777	31.369633351308924	25.068476328280592
51	20.611241330336213	23.13752396004079	29.8247112781714	26.426523431451592
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	443.0
2	686.0
3	3405.0
4	6124.0
5	3903.5
6	1683.0
7	1639.0
8	1595.0
9	1571.0
10	1547.0
11	1529.0
12	1511.0
13	1482.5
14	1454.0
15	1347.0
16	1240.0
17	1199.0
18	1158.0
19	1119.5
20	1081.0
21	1252.5
22	1424.0
23	1532.0
24	1640.0
25	1903.0
26	2654.0
27	3142.0
28	3683.5
29	4225.0
30	4896.0
31	5567.0
32	5958.5
33	6350.0
34	7358.0
35	8366.0
36	9107.5
37	9849.0
38	10635.0
39	11421.0
40	13935.5
41	16450.0
42	18086.0
43	19722.0
44	23721.5
45	27721.0
46	31042.5
47	34364.0
48	35244.5
49	36125.0
50	32090.0
51	28055.0
52	24936.0
53	21817.0
54	20578.0
55	19339.0
56	18605.5
57	17872.0
58	17679.0
59	17486.0
60	16988.0
61	16490.0
62	14835.0
63	13180.0
64	12055.5
65	10931.0
66	9274.0
67	7617.0
68	6426.5
69	5236.0
70	4444.0
71	3652.0
72	3207.5
73	2763.0
74	2256.5
75	1295.5
76	841.0
77	627.0
78	413.0
79	277.5
80	142.0
81	128.5
82	115.0
83	82.0
84	49.0
85	32.0
86	15.0
87	11.0
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.575523505003076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62474497847643	27.243865099520654
2	8.475219246467386	6.538720385043143
3	3.949061014738378	4.570112879901949
4	2.675227490427345	4.127932037528417
5	2.2560343876229747	4.351385377392265
6	1.931904081320964	4.471452677904493
7	1.7304528343244516	4.672718678934745
8	1.4991842849029138	4.626545489648286
9	1.3206630243062245	4.585074078268189
>10	5.443055845076655	28.741100311322427
>50	0.05069893603344522	1.327758564553979
>100	0.040975852410592706	3.1216793370022122
>500	0.00208351791918268	0.5001866908842371
>1k	6.945059730608934E-4	1.1214683920950275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4186	1.1175123203998056	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	819	0.21864371486083156	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	528	0.14095712020331996	TruSeq Adapter, Index 23 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	520	0.13882140626084544	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	489	0.13054551473375656	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	457	0.12200265896385838	Illumina PCR Primer Index 7 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	436	0.1163964098648627	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	391	0.10438301893844339	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	380	0.10144641226754088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1444276553598411	0.0
2	2.6696424280931813E-4	0.0	0.0	0.636175790614605	0.0
3	2.6696424280931813E-4	0.0	0.0	0.9621391310847826	0.0
4	2.6696424280931813E-4	0.0	0.0	1.41330870143253	0.0
5	2.6696424280931813E-4	0.0	0.0	2.664303143236995	0.0
6	2.6696424280931813E-4	0.0	0.0	3.7204136877906575	0.0
7	2.6696424280931813E-4	0.0	0.0	4.57710194296576	0.0
8	2.6696424280931813E-4	0.0	0.0	5.847851738738114	0.0
9	2.6696424280931813E-4	0.0	0.0	6.47948913722496	0.0
10	2.6696424280931813E-4	0.0	0.0	7.621295203720414	0.0
11	2.6696424280931813E-4	0.0	0.0	8.919275352259318	0.0
12	2.6696424280931813E-4	0.0	0.0	9.929735011292587	0.0
13	2.6696424280931813E-4	0.0	0.0	10.3598144064584	0.0
14	2.6696424280931813E-4	0.0	0.0	10.553363482495154	0.0
15	2.6696424280931813E-4	0.0	0.0	10.86784736052453	0.0
16	2.6696424280931813E-4	0.0	0.0	11.425001735267578	0.0
17	2.6696424280931813E-4	0.0	0.0	12.103357876246056	0.0
18	2.6696424280931813E-4	0.0	0.0	12.906920247102104	0.0
19	2.6696424280931813E-4	0.0	0.0	13.333796071354202	0.0
20	2.6696424280931813E-4	0.0	0.0	13.778825464117336	0.0
21	2.6696424280931813E-4	0.0	0.0	14.273510206043003	0.0
22	2.6696424280931813E-4	0.0	0.0	14.819986011073677	0.0
23	2.6696424280931813E-4	0.0	0.0	15.379810028244817	0.0
24	2.6696424280931813E-4	0.0	0.0	15.826975134950425	0.0
25	2.6696424280931813E-4	0.0	0.0	16.206064359739656	0.0
26	2.6696424280931813E-4	0.0	0.0	16.54938037599244	0.0
27	2.6696424280931813E-4	0.0	0.0	16.90951513954221	0.0
28	2.6696424280931813E-4	0.0	0.0	17.287002578874585	0.0
29	2.6696424280931813E-4	0.0	0.0	17.694390013401605	0.0
30	2.6696424280931813E-4	0.0	0.0	18.143423869806877	0.0
31	2.6696424280931813E-4	0.0	0.0	18.531589878851626	0.0
32	2.6696424280931813E-4	0.0	0.0	18.92562910123818	0.0
33	2.6696424280931813E-4	0.0	0.0	19.29777725571437	0.0
34	2.6696424280931813E-4	0.0	0.0	19.651504877436714	0.0
35	2.6696424280931813E-4	0.0	0.0	20.083186058059383	0.0
36	2.6696424280931813E-4	0.0	0.0	20.446524392522864	0.0
37	2.6696424280931813E-4	0.0	0.0	20.822943974884005	0.0
38	2.6696424280931813E-4	0.0	0.0	21.191087665718054	0.0
39	2.6696424280931813E-4	0.0	0.0	21.558697428066484	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	45	3.8380676E-10	45.000004	26
TCACGAC	50	2.1827873E-11	45.0	25
TCGTTTA	40	6.7939254E-9	45.0	38
AAATACG	20	7.02719E-4	45.0	1
CGCATCG	40	6.7939254E-9	45.0	21
TATAGCG	25	3.8857543E-5	45.0	1
CACAACG	20	7.02719E-4	45.0	12
GCTCGTC	20	7.02719E-4	45.0	27
CGTCCAT	20	7.02719E-4	45.0	30
CGTAAGG	80	0.0	42.1875	2
CGTTTTT	1630	0.0	41.68712	1
CGTTCAT	55	6.002665E-11	40.909092	17
GCGTAAG	55	6.002665E-11	40.909092	1
TAACGGG	105	0.0	40.714283	3
CGGTCTA	50	1.0768417E-9	40.5	31
TAACGCC	45	1.922308E-8	40.000004	12
ATGGTCG	45	1.922308E-8	40.000004	44
ATAACGC	45	1.922308E-8	40.000004	11
AGTCGGC	45	1.922308E-8	40.000004	30
CCAGTCG	40	3.4498953E-7	39.375	28
>>END_MODULE