##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410641
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.041125946995066	33.0	31.0	34.0	30.0	34.0
2	32.13072002065064	33.0	31.0	34.0	30.0	34.0
3	32.09095292481754	33.0	31.0	34.0	30.0	34.0
4	35.73467335214944	37.0	35.0	37.0	33.0	37.0
5	35.80349745885092	37.0	35.0	37.0	35.0	37.0
6	35.71203070321765	37.0	35.0	37.0	35.0	37.0
7	36.01890459062783	37.0	35.0	37.0	35.0	37.0
8	35.86014547987171	37.0	35.0	37.0	35.0	37.0
9	37.707333169362045	39.0	38.0	39.0	35.0	39.0
10	37.25071534503374	39.0	37.0	39.0	34.0	39.0
11	37.345679072474496	39.0	37.0	39.0	34.0	39.0
12	37.25819389685881	39.0	37.0	39.0	34.0	39.0
13	37.321358558935906	39.0	37.0	39.0	34.0	39.0
14	38.34164878811419	40.0	38.0	41.0	34.0	41.0
15	38.434335587532665	40.0	38.0	41.0	34.0	41.0
16	38.214338071454144	40.0	37.0	41.0	34.0	41.0
17	38.238524648050245	40.0	37.0	41.0	34.0	41.0
18	38.065816613538345	40.0	37.0	41.0	34.0	41.0
19	37.85027798003609	39.0	37.0	41.0	33.0	41.0
20	38.12887899649572	40.0	37.0	41.0	34.0	41.0
21	38.00887393124408	40.0	36.0	41.0	34.0	41.0
22	38.180240161113964	40.0	37.0	41.0	34.0	41.0
23	38.228301606512744	40.0	37.0	41.0	34.0	41.0
24	38.12504352950631	40.0	36.0	41.0	34.0	41.0
25	37.75081397132775	40.0	36.0	41.0	33.0	41.0
26	37.92743539977742	40.0	36.0	41.0	34.0	41.0
27	38.0345849537674	40.0	36.0	41.0	34.0	41.0
28	37.942928251197515	40.0	36.0	41.0	34.0	41.0
29	37.97967324256467	40.0	36.0	41.0	34.0	41.0
30	37.6872207110347	40.0	36.0	41.0	33.0	41.0
31	37.595016084609185	40.0	36.0	41.0	33.0	41.0
32	37.605387674391984	40.0	36.0	41.0	33.0	41.0
33	37.523773807291526	40.0	36.0	41.0	33.0	41.0
34	37.39625853239204	40.0	36.0	41.0	33.0	41.0
35	37.33874600928792	40.0	36.0	41.0	33.0	41.0
36	37.22371365742826	40.0	35.0	41.0	33.0	41.0
37	37.27033101906532	40.0	35.0	41.0	33.0	41.0
38	37.10309004702404	40.0	35.0	41.0	33.0	41.0
39	37.0607391858095	39.0	35.0	41.0	32.0	41.0
40	36.96637695700137	39.0	35.0	41.0	32.0	41.0
41	36.796096833974204	39.0	35.0	41.0	31.0	41.0
42	36.88003633343967	39.0	35.0	41.0	32.0	41.0
43	36.8400281511101	39.0	35.0	41.0	32.0	41.0
44	36.85998719075786	39.0	35.0	41.0	32.0	41.0
45	36.78892755472542	39.0	35.0	41.0	32.0	41.0
46	36.62813990809491	39.0	35.0	41.0	32.0	41.0
47	36.55105798008479	39.0	35.0	41.0	31.0	41.0
48	36.53037568094759	39.0	35.0	41.0	31.0	41.0
49	36.53342457280203	39.0	35.0	41.0	31.0	41.0
50	36.381846917380386	39.0	35.0	41.0	31.0	41.0
51	35.43612060169345	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	15.0
11	6.0
12	19.0
13	16.0
14	25.0
15	34.0
16	47.0
17	59.0
18	107.0
19	167.0
20	267.0
21	486.0
22	758.0
23	1051.0
24	1554.0
25	2285.0
26	3293.0
27	3665.0
28	4096.0
29	4339.0
30	4993.0
31	6157.0
32	8205.0
33	12079.0
34	24166.0
35	33181.0
36	30966.0
37	47396.0
38	90863.0
39	130288.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78934397685569	21.020063753984626	24.441056786828398	10.749535482331282
2	34.86329908606301	24.270348065585267	24.096960605492388	16.769392242859336
3	32.50040789887031	24.836536049736875	26.385090626605724	16.2779654247871
4	28.43529993351857	24.921768649501633	27.694993924133243	18.94793749284655
5	24.84116296229553	29.108880993373777	25.978896408298247	20.071059636032444
6	24.916167650088518	34.59201589709747	25.660857050318892	14.830959402495122
7	77.4771637513059	6.080737188931451	11.415323847350848	5.026775212411814
8	78.34970205118339	5.788511132595138	10.393506737028208	5.468280079193262
9	72.2468043863131	7.64974758974384	12.558414771053062	7.5450332528899935
10	37.65649314121093	25.51644867414603	20.926064372529776	15.900993812113256
11	30.245883874235645	25.31383860841952	24.769567578493138	19.670709938851697
12	26.628855861932934	22.7088868378949	30.759227646533105	19.903029653639067
13	24.737179190582527	25.395661904193688	31.60546560134034	18.26169330388344
14	21.48811248754995	28.673707691146284	30.338909168836036	19.499270652467725
15	19.92908647699572	25.46920546170499	34.37966496282641	20.222043098472874
16	22.80507791477227	24.600319987531687	31.822443448170056	20.772158649525984
17	22.366738830267803	24.95074773342165	29.076492605463166	23.60602083084738
18	24.37895874985693	24.066520391290688	30.422193594891887	21.13232726396049
19	23.057609931789568	26.977335434113982	28.330829118378343	21.634225515718107
20	25.039876680604227	26.423810579070285	28.43797867236832	20.09833406795717
21	23.697828516879706	28.166939005116394	28.40461619760326	19.730616280400643
22	22.282236795643882	24.957809863116445	28.10727618528106	24.65267715595861
23	21.50710718121181	27.541575244556682	28.241943692909377	22.70937388132213
24	22.938284292118908	25.979626973439085	29.131284991026224	21.950803743415783
25	21.496635747526426	27.442705428829562	27.88737607788799	23.173282745756023
26	19.91057882676109	28.629630260982218	29.049948738679284	22.409842173577406
27	22.5028674681778	27.45122868880604	28.09656122988206	21.9493426131341
28	19.629554769251	28.283585905937304	29.810710572008155	22.276148752803543
29	21.320082505156574	28.28261181908285	28.42020158727453	21.977104088486048
30	22.3272883126624	28.811540981051575	28.60746978504338	20.253700921242643
31	25.48844367708047	28.557060790325366	24.405989660068037	21.548505872526125
32	24.04557752391992	29.99237777036389	25.45410711546095	20.507937590255235
33	23.688818213476004	27.53183437601214	26.078496789166206	22.70085062134565
34	23.162324268643413	27.782418219320526	27.3197269634547	21.735530548581362
35	22.488499687074597	29.141512902997995	26.61448808082973	21.75549932909768
36	23.81374485255978	29.518728037385454	25.631390922971647	21.03613618708312
37	23.99273331206577	29.55257755557774	25.736348781539103	20.71834035081738
38	22.805564958199497	29.974113641842877	25.436086508653545	21.784234891304084
39	23.585321485190228	26.276236420620446	27.161681371319474	22.976760722869855
40	25.360351255719713	24.582542902437897	28.040307714037322	22.016798127805064
41	22.856948039771964	26.46642687895266	25.74560260665642	24.93102247461895
42	22.7500420074956	27.03748529737654	26.31933976393005	23.893132931197812
43	22.62608945526628	26.560913303834738	26.573089389515413	24.23990785138357
44	22.915880294466458	26.64103194761361	26.658565510993785	23.784522246926144
45	23.67810325807701	25.85591794292338	26.452546141276688	24.013432657722923
46	24.3192959300216	27.37817217472196	26.75280841416225	21.54972348109419
47	22.393769740478913	26.819046320265144	28.336186596077837	22.450997343178106
48	22.127600507499253	26.053414052664003	27.962380765680972	23.856604674155772
49	23.0868325374232	24.649511373681634	29.021456698186494	23.242199390708674
50	21.668318555624012	24.37798466300248	29.01536865534615	24.938328126027358
51	21.57334508731471	24.404041486359134	26.97636134725953	27.04625207906663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	419.0
2	624.0
3	2136.0
4	3648.0
5	2430.5
6	1213.0
7	1207.0
8	1201.0
9	1268.0
10	1335.0
11	1347.5
12	1360.0
13	1372.0
14	1384.0
15	1385.5
16	1387.0
17	1327.0
18	1267.0
19	1305.5
20	1344.0
21	1367.5
22	1391.0
23	1553.0
24	1715.0
25	1925.0
26	2544.5
27	2954.0
28	3364.5
29	3775.0
30	4134.5
31	4494.0
32	5648.5
33	6803.0
34	8108.5
35	9414.0
36	10125.5
37	10837.0
38	12429.5
39	14022.0
40	15963.5
41	17905.0
42	19928.5
43	21952.0
44	25491.0
45	29030.0
46	35128.5
47	41227.0
48	43597.0
49	45967.0
50	44450.0
51	42933.0
52	37929.0
53	32925.0
54	28321.0
55	23717.0
56	21234.0
57	18751.0
58	17192.5
59	15634.0
60	14629.0
61	13624.0
62	12253.5
63	10883.0
64	9524.0
65	8165.0
66	6700.5
67	5236.0
68	4356.0
69	3476.0
70	2863.5
71	2251.0
72	2038.0
73	1825.0
74	1537.5
75	977.5
76	705.0
77	545.5
78	386.0
79	289.5
80	193.0
81	119.0
82	45.0
83	36.5
84	28.0
85	21.0
86	14.0
87	7.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.34241815783543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99471759828158	27.63141686025975
2	9.73217622988853	7.268459887244877
3	3.6463139536287548	4.084865411734653
4	2.070619496214254	3.0928775629359686
5	1.427557522344407	2.665422497187417
6	1.0982254404344667	2.4606236176966103
7	0.9270879634322918	2.423379447970928
8	0.7248112841182682	2.1652964845649625
9	0.5938444449676233	1.9958028826219811
>10	5.646422677290683	39.2549540471706
>50	0.09695289872547408	2.4672916248101235
>100	0.03537470629172702	2.2405238936779615
>500	0.003930522921303003	1.0690129288538806
>1k	0.0019652614606515016	1.180072853270279
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2235	0.5442710299263834	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	1581	0.3850078292230927	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	1008	0.24546988732250313	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	977	0.23792071420048166	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	817	0.19895724002230658	No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	785	0.19116454518667156	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCC	673	0.163890113261949	No Hit
TCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	576	0.14026850704143035	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	542	0.13198876877856813	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2157602382616446	0.0
2	0.0	0.0	0.0	1.0741742787495647	0.0
3	2.4352171361359437E-4	0.0	0.0	1.4716017153669507	0.0
4	2.4352171361359437E-4	0.0	0.0	2.065794696584121	0.0
5	2.4352171361359437E-4	0.0	0.0	3.759244693053056	0.0
6	2.4352171361359437E-4	0.0	0.0	4.7433159377655905	0.0
7	2.4352171361359437E-4	0.0	0.0	5.6840403174549055	0.0
8	2.4352171361359437E-4	0.0	0.0	7.109859950662501	0.0
9	2.4352171361359437E-4	0.0	0.0	7.714280843851442	0.0
10	2.4352171361359437E-4	0.0	0.0	9.259913160156925	0.0
11	2.4352171361359437E-4	0.0	0.0	11.113600444183605	0.0
12	2.4352171361359437E-4	0.0	0.0	12.657528108493795	0.0
13	2.4352171361359437E-4	0.0	0.0	13.201068573279336	0.0
14	2.4352171361359437E-4	0.0	0.0	13.416341768113753	0.0
15	2.4352171361359437E-4	0.0	0.0	13.846888157782589	0.0
16	2.4352171361359437E-4	0.0	0.0	14.68435933089974	0.0
17	2.4352171361359437E-4	0.0	0.0	15.732233264579037	0.0
18	2.4352171361359437E-4	0.0	0.0	16.84902384321098	0.0
19	4.8704342722718873E-4	0.0	0.0	17.554749769263175	0.0
20	4.8704342722718873E-4	0.0	0.0	18.16671983557414	0.0
21	4.8704342722718873E-4	0.0	0.0	18.899720193551058	0.0
22	4.8704342722718873E-4	0.0	0.0	19.70869932617542	0.0
23	4.8704342722718873E-4	0.0	0.0	20.447787726992676	0.0
24	4.8704342722718873E-4	0.0	0.0	21.05707905445389	0.0
25	4.8704342722718873E-4	0.0	0.0	21.581137782150346	0.0
26	4.8704342722718873E-4	0.0	0.0	22.07792207792208	0.0
27	4.8704342722718873E-4	0.0	0.0	22.55985154916338	0.0
28	7.30565140840783E-4	0.0	0.0	23.06735080033411	0.0
29	7.30565140840783E-4	0.0	0.0	23.571440747514252	0.0
30	7.30565140840783E-4	0.0	0.0	24.14980481734654	0.0
31	7.30565140840783E-4	0.0	0.0	24.688961891287036	0.0
32	7.30565140840783E-4	0.0	0.0	25.20327975043895	0.0
33	7.30565140840783E-4	0.0	0.0	25.715405914168336	0.0
34	7.30565140840783E-4	0.0	0.0	26.225340382475203	0.0
35	9.740868544543775E-4	0.0	0.0	26.779108759232518	0.0
36	9.740868544543775E-4	0.0	0.0	27.26639570817332	0.0
37	9.740868544543775E-4	0.0	0.0	27.73785374572924	0.0
38	9.740868544543775E-4	0.0	0.0	28.262886560280148	0.0
39	9.740868544543775E-4	0.0	0.0	28.74676420523036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	30	2.16208E-6	45.000004	25
CGAATAT	65	0.0	45.000004	14
GCAACCG	20	7.0279854E-4	45.0	1
TAGCGGA	20	7.0279854E-4	45.0	3
GCCGCAT	20	7.0279854E-4	45.0	19
GTTACTC	20	7.0279854E-4	45.0	9
TACGCGG	20	7.0279854E-4	45.0	2
AACGCCG	20	7.0279854E-4	45.0	13
CATCGTT	20	7.0279854E-4	45.0	36
ATAACGG	20	7.0279854E-4	45.0	2
GTGCGAA	25	3.8864117E-5	45.0	1
GCATCGT	20	7.0279854E-4	45.0	35
AATCGGT	45	3.8380676E-10	45.0	26
AAATGCG	20	7.0279854E-4	45.0	1
ACGGTCG	20	7.0279854E-4	45.0	2
CGTTTTT	1340	0.0	42.145523	1
CTACGAA	75	0.0	42.0	11
ACAACGA	140	0.0	41.785717	13
CGCGACC	120	0.0	41.250004	10
CACAACG	145	0.0	40.34483	12
>>END_MODULE