FastQCFastQC Report
Sat 18 Jun 2016
SRR3551577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731212
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115231.5758767635104456No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC12540.17149609142081912No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC12280.16794035108833005No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9900.13539165112169932No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG8960.12253628222731573No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC8250.11282637593474944No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC7340.10038128477103767No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA207.0316E-445.00000420
TCGAACG207.0316E-445.0000041
CGTTTTT46100.042.7060741
TACGAAT750.042.012
TACGGGA1550.040.645164
TATAGCG501.0804797E-940.51
GCGTAAG501.0804797E-940.51
GTTAGCG451.9266736E-840.01
CTAACGG451.9266736E-840.02
TCGACAC451.9266736E-840.034
CGAATAT800.039.37500414
ACGGGCT2450.037.653065
ATTAGCG301.13951566E-437.5000041
ATTACGG301.13951566E-437.5000042
CGTAAGA301.13951566E-437.50000412
AATATAC850.037.05882316
TATTAGT552.746674E-936.81818430
GTTTTTT55900.036.4266552
CGTTACC504.8748916E-836.037
CGTTAAA250.002106608436.036