##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731212
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97032734692538	33.0	31.0	34.0	30.0	34.0
2	32.08240838498274	33.0	31.0	34.0	30.0	34.0
3	32.01235209487809	33.0	31.0	34.0	30.0	34.0
4	35.68607872956133	37.0	35.0	37.0	33.0	37.0
5	35.72859991356816	37.0	35.0	37.0	35.0	37.0
6	35.59980279317079	37.0	35.0	37.0	33.0	37.0
7	35.90242091212945	37.0	35.0	37.0	35.0	37.0
8	35.7085756251265	37.0	35.0	37.0	35.0	37.0
9	37.553416792941036	39.0	37.0	39.0	35.0	39.0
10	37.19797541615838	39.0	37.0	39.0	34.0	39.0
11	37.27470008697888	39.0	37.0	39.0	34.0	39.0
12	37.226830248956524	39.0	37.0	39.0	34.0	39.0
13	37.32871451781426	39.0	37.0	39.0	34.0	39.0
14	38.43539766852842	40.0	38.0	41.0	34.0	41.0
15	38.50099423970066	40.0	38.0	41.0	34.0	41.0
16	38.24581243196228	40.0	38.0	41.0	34.0	41.0
17	38.25674770107712	40.0	37.0	41.0	34.0	41.0
18	38.00975503684294	39.0	37.0	41.0	33.0	41.0
19	37.76945947276577	39.0	37.0	41.0	33.0	41.0
20	37.98904011422132	40.0	36.0	41.0	34.0	41.0
21	37.835250242063864	40.0	36.0	41.0	33.0	41.0
22	37.992869373040925	40.0	36.0	41.0	34.0	41.0
23	38.0346465867628	40.0	36.0	41.0	34.0	41.0
24	37.87598535034983	40.0	36.0	41.0	34.0	41.0
25	37.49579738844548	39.0	35.0	41.0	33.0	41.0
26	37.66691465676165	39.0	35.0	41.0	33.0	41.0
27	37.76132913573628	40.0	36.0	41.0	33.0	41.0
28	37.649438193027464	40.0	35.0	41.0	33.0	41.0
29	37.64568278419939	40.0	35.0	41.0	33.0	41.0
30	37.329174028872615	39.0	35.0	41.0	33.0	41.0
31	37.193648353692225	39.0	35.0	41.0	33.0	41.0
32	37.112459861162016	39.0	35.0	41.0	32.0	41.0
33	36.97634065086459	40.0	35.0	41.0	32.0	41.0
34	36.7526066311822	39.0	35.0	41.0	31.0	41.0
35	36.64321838263048	39.0	35.0	41.0	31.0	41.0
36	36.458519553836645	39.0	35.0	41.0	30.0	41.0
37	36.44188005667303	39.0	35.0	41.0	30.0	41.0
38	36.2518106923847	39.0	35.0	41.0	30.0	41.0
39	36.15842464292162	39.0	35.0	41.0	30.0	41.0
40	36.07233469910231	39.0	35.0	41.0	29.0	41.0
41	35.880570887786305	39.0	35.0	41.0	29.0	41.0
42	35.92337379583486	39.0	35.0	41.0	29.0	41.0
43	35.88513727892868	39.0	35.0	41.0	29.0	41.0
44	35.90756716246451	39.0	35.0	41.0	29.0	41.0
45	35.85289218448275	38.0	35.0	41.0	29.0	41.0
46	35.74530779035355	38.0	35.0	40.0	29.0	41.0
47	35.672653074621316	38.0	35.0	40.0	29.0	41.0
48	35.593622642954436	38.0	35.0	40.0	29.0	41.0
49	35.52820932916856	38.0	35.0	40.0	29.0	41.0
50	35.37489811436355	37.0	35.0	40.0	28.0	41.0
51	34.50706908529948	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	16.0
9	39.0
10	41.0
11	44.0
12	40.0
13	44.0
14	65.0
15	89.0
16	121.0
17	154.0
18	297.0
19	475.0
20	786.0
21	1218.0
22	1712.0
23	2490.0
24	3627.0
25	6013.0
26	8684.0
27	10874.0
28	10928.0
29	10713.0
30	11344.0
31	13435.0
32	16702.0
33	23205.0
34	41475.0
35	54690.0
36	61104.0
37	90403.0
38	153851.0
39	206437.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.682986603064506	20.004184832852854	22.670716563732544	15.642112000350103
2	37.161726011061084	23.15552808214307	23.574011367428323	16.108734539367518
3	31.94873716514499	22.807339048046256	29.56064725414791	15.683276532660843
4	28.545073111491604	23.954065305273982	28.724364479795188	18.776497103439223
5	24.865702422826757	28.7188941100529	27.039080321438924	19.37632314568142
6	25.819051109664503	33.42149745901325	26.611297407591778	14.148154023730463
7	75.36596773575927	6.673440807864203	13.371635038812274	4.588956417564264
8	76.39467076579706	6.292429555313643	12.410764593578879	4.902135085310416
9	69.80437957801567	8.100113236653666	14.898688752372774	7.196818432957884
10	37.648178640394306	24.619399025180112	21.476534848990443	16.255887485435142
11	28.13766732493449	23.6224241396476	27.752006258102984	20.487902277314923
12	25.323298851769394	21.352494215083997	32.6503120845938	20.673894848552813
13	24.15687926346942	21.61903798077712	33.97140637735704	20.252676378396416
14	21.16226210729583	25.32603403664054	31.830440419467955	21.68126343659568
15	19.92007789806513	23.598080994294403	34.88974469784413	21.592096409796337
16	22.53997472689179	22.4874591773658	33.408368571631755	21.564197524110654
17	22.47939038199592	23.029162541096152	30.970635055223383	23.520812021684545
18	22.671537119193886	23.421114533131295	30.966669037160223	22.940679310514597
19	24.014102613195625	24.725797716667667	29.448778192918056	21.811321477218645
20	25.181753034687617	23.712821999638955	29.321181818679126	21.784243146994307
21	24.747268917906162	24.94598009879488	29.178268409161774	21.128482574137188
22	23.50344359775277	23.048445594437727	28.992139078680328	24.45597172912917
23	23.06649781458729	24.677384944448395	29.669370852775938	22.586746388188377
24	22.6548524914799	24.401678309436935	30.512765107793637	22.43070409128953
25	24.073182606412367	24.618988747449443	28.842524466228674	22.46530417990952
26	22.17209236172273	24.881976772810074	29.34634551949366	23.599585345973534
27	23.84452115118461	23.909481791874313	29.54191123778056	22.704085819160515
28	22.84344348834538	24.76244919394102	29.868902589126	22.525204728587607
29	24.122826211823657	24.645246522212435	28.495429506080317	22.73649775988359
30	25.046224624322356	23.932047067061262	28.617008473602727	22.404719835013648
31	25.379780419358543	24.583978381098778	26.315623923020958	23.72061727652172
32	25.186949885942788	25.743970284951562	26.400551413270023	22.668528415835627
33	24.999589722269327	25.403986805468183	26.00162469981346	23.59479877244903
34	23.77436365923973	25.795391760529096	26.70087471212179	23.729369868109384
35	24.878968069451815	25.135391651121697	26.115271631209552	23.87036864821693
36	25.472913464221048	26.42421076240543	25.80988824034616	22.292987533027357
37	24.006170577069305	27.793717827387955	26.102142743828054	22.097968851714686
38	22.96556949284202	27.15778734484664	26.411902430485277	23.464740731826065
39	23.636236823246882	25.69063417996422	27.270750480024947	23.402378516763946
40	23.332357784062623	24.711164477607042	30.028500626357335	21.927977111973
41	22.322664288879285	25.132109429276323	28.642308933660825	23.902917348183564
42	22.431934924481546	26.133734129089785	27.805615881577435	23.62871506485123
43	22.863957374878968	24.120501304683184	28.93346936319426	24.08207195724359
44	23.971570488449316	24.552523755080603	28.304240083587253	23.17166567288283
45	23.364085928567913	24.56975541976882	28.020191134718797	24.04596751694447
46	24.29117684064266	24.869941959377034	27.654223398959537	23.184657801020773
47	23.122295585958653	25.110227950307163	29.06202305213809	22.705453411596093
48	22.309261883010674	24.891960197589754	28.678686892447057	24.12009102695251
49	22.48089473367505	24.521069129062433	29.54505670038238	23.45297943688014
50	21.721470654201518	23.97922900608852	30.215997549274356	24.083302790435607
51	22.475834641663432	24.152502967675584	28.77428160369359	24.597380786967392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	321.0
1	776.5
2	1232.0
3	9028.5
4	16825.0
5	10859.5
6	4894.0
7	4726.5
8	4559.0
9	4390.0
10	4221.0
11	4133.0
12	4045.0
13	3863.5
14	3682.0
15	3465.5
16	3249.0
17	3057.0
18	2865.0
19	2726.0
20	2587.0
21	2617.5
22	2648.0
23	2888.5
24	3129.0
25	3661.5
26	4820.5
27	5447.0
28	6812.0
29	8177.0
30	9620.5
31	11064.0
32	11717.5
33	12371.0
34	13055.0
35	13739.0
36	15776.5
37	17814.0
38	18567.5
39	19321.0
40	22005.0
41	24689.0
42	26800.5
43	28912.0
44	32461.0
45	36010.0
46	38962.0
47	41914.0
48	49228.5
49	56543.0
50	58130.5
51	59718.0
52	59379.5
53	59041.0
54	53423.5
55	47806.0
56	44451.0
57	41096.0
58	39785.5
59	38475.0
60	37070.5
61	35666.0
62	33326.5
63	30987.0
64	28054.0
65	25121.0
66	21952.5
67	18784.0
68	16187.5
69	13591.0
70	11524.0
71	9457.0
72	8256.5
73	7056.0
74	6005.5
75	3645.5
76	2336.0
77	1798.0
78	1260.0
79	987.5
80	715.0
81	563.5
82	412.0
83	300.5
84	189.0
85	116.0
86	43.0
87	35.5
88	28.0
89	16.5
90	5.0
91	6.5
92	8.0
93	9.5
94	11.0
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731212.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.88714559100736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6734273468663	22.446747310601552
2	7.281218198779578	4.497920931711944
3	3.0912749675415174	2.864419799528742
4	1.9995043167599271	2.470359237664463
5	1.5299601105748537	2.362805034187961
6	1.2451840986492728	2.3076109525552417
7	1.1106278096220374	2.4012885997252233
8	1.0053557073354593	2.484205448257641
9	0.9273071385054531	2.577768353513852
>10	9.010796816650867	47.94141449675566
>50	0.06958570905837737	1.4996905922223112
>100	0.04995897057215614	3.245724478962543
>500	0.004460622372513942	0.9704933878008385
>1k	8.921244745027884E-4	0.34195976554822277
>5k	0.0	0.0
>10k+	4.460622372513942E-4	1.587591610963808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11523	1.5758767635104456	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	1254	0.17149609142081912	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1228	0.16794035108833005	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	990	0.13539165112169932	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	896	0.12253628222731573	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	825	0.11282637593474944	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCC	734	0.10038128477103767	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.102777306718161E-4	0.0	0.0	0.09573147049009043	0.0
2	4.102777306718161E-4	0.0	0.0	0.3992002319436771	0.0
3	4.102777306718161E-4	0.0	0.0	0.6278616871714359	0.0
4	4.102777306718161E-4	0.0	0.0	0.9333818372783816	0.0
5	4.102777306718161E-4	0.0	0.0	1.8013927561363872	0.0
6	4.102777306718161E-4	0.0	0.0	2.692242468668457	0.0
7	4.102777306718161E-4	0.0	0.0	3.356208596139013	0.0
8	4.102777306718161E-4	0.0	0.0	4.455616155095923	0.0
9	4.102777306718161E-4	0.0	0.0	5.008533776797973	0.0
10	4.102777306718161E-4	0.0	0.0	5.969130703544253	0.0
11	4.102777306718161E-4	0.0	0.0	6.824423012751432	0.0
12	4.102777306718161E-4	0.0	0.0	7.556632002757066	0.0
13	4.102777306718161E-4	0.0	0.0	7.855997986903935	0.0
14	4.102777306718161E-4	0.0	0.0	8.003697969945788	0.0
15	4.102777306718161E-4	0.0	0.0	8.211845538639956	0.0
16	4.102777306718161E-4	0.0	0.0	8.573847256336055	0.0
17	4.102777306718161E-4	0.0	0.0	8.999715540773401	0.0
18	4.102777306718161E-4	0.0	0.0	9.501758723872147	0.0
19	5.470369742290881E-4	0.0	0.0	9.788132579881074	0.0
20	5.470369742290881E-4	0.0	0.0	10.046470790960761	0.0
21	5.470369742290881E-4	0.0	0.0	10.340639923852454	0.0
22	5.470369742290881E-4	0.0	0.0	10.66667396049299	0.0
23	5.470369742290881E-4	0.0	0.0	10.97096327740792	0.0
24	5.470369742290881E-4	0.0	0.0	11.239968709485074	0.0
25	5.470369742290881E-4	0.0	0.0	11.476425441595598	0.0
26	5.470369742290881E-4	0.0	0.0	11.709599951860746	0.0
27	5.470369742290881E-4	0.0	0.0	11.93046613020574	0.0
28	5.470369742290881E-4	0.0	0.0	12.16459795517579	0.0
29	5.470369742290881E-4	0.0	0.0	12.402148761234772	0.0
30	5.470369742290881E-4	0.0	0.0	12.688112339513028	0.0
31	5.470369742290881E-4	0.0	0.0	12.934552496403231	0.0
32	5.470369742290881E-4	0.0	0.0	13.197677281007424	0.0
33	5.470369742290881E-4	0.0	0.0	13.457109566035568	0.0
34	6.837962177863602E-4	0.0	0.0	13.71476398089747	0.0
35	6.837962177863602E-4	0.0	0.0	13.994163115484975	0.0
36	8.205554613436322E-4	0.0	0.0	14.24962938244996	0.0
37	9.573147049009043E-4	0.0	0.0	14.515215833438182	0.0
38	9.573147049009043E-4	0.0	0.0	14.774784877709884	0.0
39	9.573147049009043E-4	0.0	0.0	15.035311236686487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACA	20	7.0316E-4	45.000004	20
TCGAACG	20	7.0316E-4	45.000004	1
CGTTTTT	4610	0.0	42.706074	1
TACGAAT	75	0.0	42.0	12
TACGGGA	155	0.0	40.64516	4
TATAGCG	50	1.0804797E-9	40.5	1
GCGTAAG	50	1.0804797E-9	40.5	1
GTTAGCG	45	1.9266736E-8	40.0	1
CTAACGG	45	1.9266736E-8	40.0	2
TCGACAC	45	1.9266736E-8	40.0	34
CGAATAT	80	0.0	39.375004	14
ACGGGCT	245	0.0	37.65306	5
ATTAGCG	30	1.13951566E-4	37.500004	1
ATTACGG	30	1.13951566E-4	37.500004	2
CGTAAGA	30	1.13951566E-4	37.500004	12
AATATAC	85	0.0	37.058823	16
TATTAGT	55	2.746674E-9	36.818184	30
GTTTTTT	5590	0.0	36.426655	2
CGTTACC	50	4.8748916E-8	36.0	37
CGTTAAA	25	0.0021066084	36.0	36
>>END_MODULE