FastQCFastQC Report
Sat 18 Jun 2016
SRR3551576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472903
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56001.184175190261005No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC6780.1433697819637431Illumina PCR Primer Index 8 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5430.11482270148423673No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5240.11080496423156545No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5110.1080559861113167No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC5070.10721014668970169No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACATA253.887311E-545.0000049
ACCGGTA351.2099008E-745.041
TAACGCC351.2099008E-745.012
CAATCGC207.0290704E-445.011
CGCATCG302.162782E-644.99999621
ATAACGC302.162782E-644.99999611
CGAATAT850.042.3529414
CGTTTAC650.041.53846433
CGTTTTT23650.041.4799161
GTAACGG603.6379788E-1241.2499962
ATAGGGT556.002665E-1140.909094
CGGTCTA501.0786607E-940.50000431
CTCCTTA501.0786607E-940.50000441
CTACGAA950.040.26315711
GCGAATG451.924127E-840.01
ACATACG850.039.70588317
TCGTTTA356.2404924E-638.57142638
GCGTTTA700.038.57142632
GCGTTAG356.2404924E-638.5714261
GCCGATT356.2404924E-638.5714269