##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	472903
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06543413765613	33.0	31.0	34.0	30.0	34.0
2	32.179493469062365	33.0	31.0	34.0	30.0	34.0
3	32.1239154752666	33.0	31.0	34.0	30.0	34.0
4	35.75783828819018	37.0	35.0	37.0	35.0	37.0
5	35.7914456030095	37.0	35.0	37.0	35.0	37.0
6	35.675519081080054	37.0	35.0	37.0	35.0	37.0
7	35.97024548374614	37.0	35.0	37.0	35.0	37.0
8	35.78111367447447	37.0	35.0	37.0	35.0	37.0
9	37.64807793564431	39.0	37.0	39.0	35.0	39.0
10	37.315428322510115	39.0	37.0	39.0	34.0	39.0
11	37.36769485497026	39.0	37.0	39.0	35.0	39.0
12	37.281738538347184	39.0	37.0	39.0	34.0	39.0
13	37.35898693812473	39.0	37.0	39.0	35.0	39.0
14	38.45677443365764	40.0	38.0	41.0	34.0	41.0
15	38.53983586486024	40.0	38.0	41.0	34.0	41.0
16	38.27546452443736	40.0	38.0	41.0	34.0	41.0
17	38.30427804433467	40.0	38.0	41.0	34.0	41.0
18	38.078498127522984	40.0	37.0	41.0	34.0	41.0
19	37.8481401048418	39.0	37.0	41.0	33.0	41.0
20	38.11988927961971	40.0	37.0	41.0	34.0	41.0
21	37.96494841436827	40.0	36.0	41.0	34.0	41.0
22	38.100813486063736	40.0	36.0	41.0	34.0	41.0
23	38.15186412435531	40.0	36.0	41.0	34.0	41.0
24	38.02025996874623	40.0	36.0	41.0	34.0	41.0
25	37.62885623478811	40.0	35.0	41.0	33.0	41.0
26	37.79454137529261	40.0	36.0	41.0	34.0	41.0
27	37.89495943142674	40.0	36.0	41.0	34.0	41.0
28	37.77597519998816	40.0	36.0	41.0	33.0	41.0
29	37.785196964282314	40.0	36.0	41.0	33.0	41.0
30	37.49986572299182	40.0	35.0	41.0	33.0	41.0
31	37.39382283470395	40.0	35.0	41.0	33.0	41.0
32	37.364994512616754	40.0	35.0	41.0	33.0	41.0
33	37.25048265711996	40.0	35.0	41.0	33.0	41.0
34	37.073412518000524	40.0	35.0	41.0	32.0	41.0
35	36.98363512179031	40.0	35.0	41.0	32.0	41.0
36	36.79897568846042	40.0	35.0	41.0	31.0	41.0
37	36.826772086453246	40.0	35.0	41.0	32.0	41.0
38	36.63533536475768	39.0	35.0	41.0	31.0	41.0
39	36.5388990977008	39.0	35.0	41.0	31.0	41.0
40	36.43051957801071	39.0	35.0	41.0	31.0	41.0
41	36.27618771714284	39.0	35.0	41.0	30.0	41.0
42	36.33454429343861	39.0	35.0	41.0	31.0	41.0
43	36.31429278308659	39.0	35.0	41.0	31.0	41.0
44	36.307610651655835	39.0	35.0	41.0	31.0	41.0
45	36.26209391778018	39.0	35.0	41.0	31.0	41.0
46	36.1371147994409	39.0	35.0	41.0	30.0	41.0
47	36.091832786004744	38.0	35.0	41.0	30.0	41.0
48	36.02135533079723	38.0	35.0	41.0	30.0	41.0
49	35.96263292895161	38.0	35.0	40.0	30.0	41.0
50	35.832585117878295	38.0	35.0	40.0	30.0	41.0
51	34.99972721678653	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	19.0
10	27.0
11	23.0
12	27.0
13	38.0
14	31.0
15	47.0
16	82.0
17	105.0
18	171.0
19	269.0
20	405.0
21	616.0
22	919.0
23	1333.0
24	2088.0
25	3224.0
26	4784.0
27	5863.0
28	5938.0
29	6162.0
30	6628.0
31	7859.0
32	9987.0
33	14123.0
34	27530.0
35	36090.0
36	37903.0
37	56778.0
38	98640.0
39	145110.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.603464135351224	21.215344372947516	23.794520229307068	14.386671262394191
2	36.46075410813634	23.361027525729376	23.569526943157477	16.608691422976804
3	32.941004814940904	22.586872995096247	28.320395514513546	16.1517266754493
4	29.104277198495254	24.856471623144706	27.000040177372526	19.039211000987518
5	25.292078925276428	29.059870628860462	26.290803822348348	19.357246623514758
6	26.405203604121773	33.38168715360232	25.344943889127368	14.868165353148532
7	76.93734233024531	6.746626686656672	11.60956898137673	4.706462001721283
8	77.72249277335945	6.1737819383679104	11.11961649640624	4.984108791866408
9	70.72169134050746	8.938619547771953	13.64085235238516	6.698836759335423
10	39.032317409701356	25.416417320253835	19.939607065296688	15.61165820474812
11	30.509216477797775	23.40902891290603	25.764903161959218	20.31685144733698
12	26.587904919190613	21.704028098785585	31.223527869351642	20.484539112672152
13	25.109800529918395	22.08423291880153	32.780506784689464	20.02545976659061
14	22.06668175080302	25.407958926037686	31.16685662810344	21.358502695055854
15	21.472902476829287	24.036218844033556	33.274477006912626	21.216401672224535
16	23.33015438684043	22.92880358128411	32.51935386326583	21.22168816860963
17	23.637194096886677	23.329942926985026	29.799134283351975	23.23372869277632
18	24.169861472648723	23.39211212447373	30.06324764275126	22.374778760126286
19	25.18825213627319	24.968333886653287	28.473915369536666	21.36949860753685
20	25.901294768694637	24.373285853547134	28.38087303315902	21.34454634459921
21	25.344309509561157	25.007242500047578	28.880552671478082	20.767895318913183
22	23.988851836423112	23.84209869677291	28.120354491301597	24.04869497550238
23	23.6496702283555	24.71881125727686	28.605020479886996	23.026498034480642
24	23.30816256187844	24.269670524399295	29.31383391520037	23.108332998521895
25	24.372651473980923	24.323804247382654	28.47243515054884	22.83110912808758
26	22.752657521732786	25.695967249097595	28.170259017176885	23.381116211992733
27	24.829616221508427	23.686252783340347	28.80717610165298	22.676954893498245
28	23.529561030486168	25.372856590040666	28.65809690359334	22.43948547587983
29	23.838926798941852	25.51686075157062	27.84799419754157	22.79621825194596
30	24.64585760716257	25.25422761115916	27.692782663675214	22.407132118003055
31	26.437768421853953	25.37708578714874	25.788375205909038	22.396770585088273
32	25.77695637371723	26.385326377713824	25.724937249287912	22.112779999281038
33	25.80508053448593	25.94379819963079	25.037481259370313	23.213640006512964
34	24.111075632846482	26.83531295001301	25.996451703626327	23.05715971351419
35	25.419800677940295	26.13094017166311	25.894528053321718	22.554731097074875
36	25.94083776165514	27.712871349938574	24.5574673876038	21.78882350080249
37	25.865980972842213	27.93617295724493	24.872754032010793	21.325092037902067
38	25.13073505560337	27.72513602155199	24.830462060930042	22.313666861914598
39	24.969179726074902	26.18951455160995	25.712461117819085	23.12884460449606
40	23.754977236346566	26.193320829007217	28.260763835289687	21.790938099356527
41	22.619226352973023	26.476254115537433	26.880353899214004	24.02416563227554
42	23.51793073843896	26.27832769087953	26.559569298566515	23.644172272115
43	24.230761911005004	23.91653256587503	27.447066311696055	24.405639211423907
44	24.451526000046524	24.777597097079106	27.356350033727843	23.414526869146528
45	24.000270668614917	24.94591914198049	26.918628133042084	24.13518205636251
46	24.289547750807248	25.753484329767417	27.04106338932085	22.915904530104484
47	23.960727675654415	25.901083308839233	27.963239818736614	22.174949196769738
48	23.361450445440184	25.358265860017802	27.883942372960206	23.396341321581804
49	23.68160066652146	25.009780018312423	28.153130768889177	23.15548854627693
50	22.620072192394638	24.596164541142688	28.90169865701846	23.88206460944422
51	23.181498108491596	24.957972353738505	27.409849377144997	24.45068016062491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	619.5
2	947.0
3	4771.0
4	8595.0
5	5538.5
6	2482.0
7	2443.0
8	2404.0
9	2354.5
10	2305.0
11	2137.0
12	1969.0
13	1971.5
14	1974.0
15	1895.0
16	1816.0
17	1733.5
18	1651.0
19	1612.5
20	1574.0
21	1629.5
22	1685.0
23	1707.5
24	1730.0
25	2249.5
26	3157.0
27	3545.0
28	4287.5
29	5030.0
30	5926.0
31	6822.0
32	7684.0
33	8546.0
34	9023.5
35	9501.0
36	9894.5
37	10288.0
38	11167.0
39	12046.0
40	13994.0
41	15942.0
42	17667.0
43	19392.0
44	21568.0
45	23744.0
46	25807.5
47	27871.0
48	31419.0
49	34967.0
50	36927.0
51	38887.0
52	39263.5
53	39640.0
54	36177.5
55	32715.0
56	30250.5
57	27786.0
58	26939.5
59	26093.0
60	24718.0
61	23343.0
62	21828.0
63	20313.0
64	18367.0
65	16421.0
66	14134.5
67	11848.0
68	10543.0
69	9238.0
70	7803.0
71	6368.0
72	5421.0
73	4474.0
74	3617.5
75	2098.0
76	1435.0
77	1183.0
78	931.0
79	699.0
80	467.0
81	319.0
82	171.0
83	120.0
84	69.0
85	49.0
86	29.0
87	23.0
88	17.0
89	11.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	472903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.02324439839517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35902479407457	24.278655405999398
2	7.70243557925834	5.241236963520021
3	3.360958619575015	3.4305214958008077
4	2.22624047251768	3.0297569474427104
5	1.82131544132875	3.0983530193449513
6	1.5416733141964867	3.147163677083456
7	1.3582833350373822	3.234924410917089
8	1.2839399331055554	3.494704170952757
9	1.1791654959498803	3.610713226937186
>10	8.06272710561586	41.6197140766762
>50	0.06428921354534539	1.5137156610087832
>100	0.03620169306436925	2.5245583302570727
>500	0.0031208356089973497	0.5867559443556774
>1k	0.0	0.0
>5k	6.241671217994698E-4	1.18922666970387
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5600	1.184175190261005	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	678	0.1433697819637431	Illumina PCR Primer Index 8 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	543	0.11482270148423673	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	524	0.11080496423156545	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	511	0.1080559861113167	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	507	0.10721014668970169	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10171219044920417	0.0
2	0.0	0.0	0.0	0.4341270831439005	0.0
3	0.0	0.0	0.0	0.6699048219190827	0.0
4	0.0	0.0	0.0	0.9393046777034614	0.0
5	0.0	0.0	0.0	1.6819516898814344	0.0
6	0.0	0.0	0.0	2.4527228628281064	0.0
7	0.0	0.0	0.0	3.0215498738641964	0.0
8	0.0	0.0	0.0	3.942880463858339	0.0
9	0.0	0.0	0.0	4.417396379384356	0.0
10	0.0	0.0	0.0	5.207621859028173	0.0
11	0.0	0.0	0.0	6.232990697880961	0.0
12	0.0	0.0	0.0	7.000378513141173	0.0
13	0.0	0.0	0.0	7.334062164968292	0.0
14	0.0	0.0	0.0	7.49181121709949	0.0
15	0.0	0.0	0.0	7.750215160402873	0.0
16	2.1145985540375087E-4	0.0	0.0	8.177786988029258	0.0
17	2.1145985540375087E-4	0.0	0.0	8.657800859795772	0.0
18	4.2291971080750173E-4	0.0	0.0	9.210979841531984	0.0
19	4.2291971080750173E-4	0.0	0.0	9.516750792445809	0.0
20	6.343795662112526E-4	0.0	0.0	9.849165685140505	0.0
21	6.343795662112526E-4	0.0	0.0	10.232119483276698	0.0
22	6.343795662112526E-4	0.0	0.0	10.607460726618354	0.0
23	6.343795662112526E-4	0.0	0.0	10.95510072890212	0.0
24	6.343795662112526E-4	0.0	0.0	11.281806205500917	0.0
25	6.343795662112526E-4	0.0	0.0	11.573197886247286	0.0
26	6.343795662112526E-4	0.0	0.0	11.83836854492359	0.0
27	8.458394216150035E-4	0.0	0.0	12.109882999262005	0.0
28	8.458394216150035E-4	0.0	0.0	12.383934971865266	0.0
29	8.458394216150035E-4	0.0	0.0	12.651220229095607	0.0
30	8.458394216150035E-4	0.0	0.0	12.983846581645707	0.0
31	8.458394216150035E-4	0.0	0.0	13.269528846296176	0.0
32	8.458394216150035E-4	0.0	0.0	13.563458045307389	0.0
33	8.458394216150035E-4	0.0	0.0	13.855061185909161	0.0
34	8.458394216150035E-4	0.0	0.0	14.126575640247577	0.0
35	8.458394216150035E-4	0.0	0.0	14.427483014487114	0.0
36	8.458394216150035E-4	0.0	0.0	14.70576418419845	0.0
37	8.458394216150035E-4	0.0	0.0	14.982988054632768	0.0
38	8.458394216150035E-4	0.0	0.0	15.285164188004728	0.0
39	8.458394216150035E-4	0.0	0.0	15.585225722822651	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACATA	25	3.887311E-5	45.000004	9
ACCGGTA	35	1.2099008E-7	45.0	41
TAACGCC	35	1.2099008E-7	45.0	12
CAATCGC	20	7.0290704E-4	45.0	11
CGCATCG	30	2.162782E-6	44.999996	21
ATAACGC	30	2.162782E-6	44.999996	11
CGAATAT	85	0.0	42.35294	14
CGTTTAC	65	0.0	41.538464	33
CGTTTTT	2365	0.0	41.479916	1
GTAACGG	60	3.6379788E-12	41.249996	2
ATAGGGT	55	6.002665E-11	40.90909	4
CGGTCTA	50	1.0786607E-9	40.500004	31
CTCCTTA	50	1.0786607E-9	40.500004	41
CTACGAA	95	0.0	40.263157	11
GCGAATG	45	1.924127E-8	40.0	1
ACATACG	85	0.0	39.705883	17
TCGTTTA	35	6.2404924E-6	38.571426	38
GCGTTTA	70	0.0	38.571426	32
GCGTTAG	35	6.2404924E-6	38.571426	1
GCCGATT	35	6.2404924E-6	38.571426	9
>>END_MODULE