##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498933
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05058194186394	33.0	31.0	34.0	30.0	34.0
2	32.154818382428104	33.0	31.0	34.0	30.0	34.0
3	32.08036149142269	33.0	31.0	34.0	30.0	34.0
4	35.7383817065618	37.0	35.0	37.0	33.0	37.0
5	35.81552032036365	37.0	35.0	37.0	35.0	37.0
6	35.74009937205997	37.0	35.0	37.0	35.0	37.0
7	36.07695221602901	37.0	35.0	37.0	35.0	37.0
8	35.93885351339759	37.0	35.0	37.0	35.0	37.0
9	37.80714043769404	39.0	38.0	39.0	35.0	39.0
10	37.416085927369004	39.0	37.0	39.0	34.0	39.0
11	37.414061607470344	39.0	37.0	39.0	34.0	39.0
12	37.424121074372714	39.0	37.0	39.0	35.0	39.0
13	37.53123766116894	39.0	37.0	39.0	35.0	39.0
14	38.62578743037643	40.0	38.0	41.0	35.0	41.0
15	38.68563113684603	40.0	38.0	41.0	35.0	41.0
16	38.47350044996021	40.0	38.0	41.0	34.0	41.0
17	38.50184894565002	40.0	38.0	41.0	34.0	41.0
18	38.2678896765698	40.0	38.0	41.0	34.0	41.0
19	38.02951097642369	40.0	37.0	41.0	34.0	41.0
20	38.25830121479237	40.0	37.0	41.0	34.0	41.0
21	38.1334287369246	40.0	37.0	41.0	34.0	41.0
22	38.31793246788647	40.0	37.0	41.0	34.0	41.0
23	38.346110600020445	40.0	37.0	41.0	35.0	41.0
24	38.238707401595	40.0	37.0	41.0	34.0	41.0
25	37.89504001539285	40.0	36.0	41.0	34.0	41.0
26	38.114424181202686	40.0	37.0	41.0	34.0	41.0
27	38.2035463679492	40.0	37.0	41.0	34.0	41.0
28	38.11457650626437	40.0	37.0	41.0	34.0	41.0
29	38.127638380303566	40.0	37.0	41.0	34.0	41.0
30	37.84194671428829	40.0	36.0	41.0	34.0	41.0
31	37.69717978165405	40.0	36.0	41.0	33.0	41.0
32	37.61276163332552	40.0	36.0	41.0	33.0	41.0
33	37.47982394429713	40.0	36.0	41.0	33.0	41.0
34	37.305111107102555	40.0	36.0	41.0	33.0	41.0
35	37.21617531812889	40.0	36.0	41.0	33.0	41.0
36	37.0694882880066	40.0	36.0	41.0	32.0	41.0
37	37.06258355330275	40.0	36.0	41.0	32.0	41.0
38	36.889864971849924	40.0	35.0	41.0	31.0	41.0
39	36.843163310504615	40.0	35.0	41.0	31.0	41.0
40	36.80917077042408	40.0	35.0	41.0	31.0	41.0
41	36.67213433467018	39.0	35.0	41.0	31.0	41.0
42	36.769049150887994	40.0	35.0	41.0	31.0	41.0
43	36.72748044326593	40.0	35.0	41.0	31.0	41.0
44	36.72596120120337	40.0	35.0	41.0	31.0	41.0
45	36.68977999049973	39.0	35.0	41.0	31.0	41.0
46	36.6055382185584	39.0	35.0	41.0	31.0	41.0
47	36.571605806791695	39.0	35.0	41.0	31.0	41.0
48	36.53458279969455	39.0	35.0	41.0	31.0	41.0
49	36.5157806759625	39.0	35.0	41.0	31.0	41.0
50	36.39343158299812	39.0	35.0	41.0	31.0	41.0
51	35.544237402617185	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	16.0
11	17.0
12	9.0
13	12.0
14	24.0
15	29.0
16	31.0
17	56.0
18	92.0
19	190.0
20	361.0
21	560.0
22	881.0
23	1356.0
24	2195.0
25	3842.0
26	5419.0
27	6106.0
28	5910.0
29	5647.0
30	6011.0
31	6986.0
32	9023.0
33	12850.0
34	23283.0
35	33636.0
36	35406.0
37	53789.0
38	110902.0
39	174209.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.5061721714138	20.86833302267038	25.167707888634343	15.45778691728148
2	35.82084167613688	24.104038017128552	24.99052979057308	15.084590516161489
3	30.34655955809698	23.10109774258267	31.231848765265074	15.320493934055273
4	26.418577243838353	24.490262219576575	31.36914174849128	17.722018788093795
5	24.53876572605941	27.884906390236765	28.836737598034208	18.739590285669617
6	25.10798043023813	32.35704994458174	27.422519656947923	15.112449968232209
7	77.30035896603351	4.744123960531775	13.398993452026625	4.556523621408084
8	78.10527666039327	4.621863055760994	12.170972856074863	5.101887427770864
9	71.46390397107427	7.50361270952212	14.395920895190336	6.636562424213271
10	32.54364814514174	34.31021800522315	20.345417120134368	12.800716729500754
11	24.110050848510724	23.673318862452476	33.74360886131004	18.473021427726767
12	23.054999368652705	20.496138760114082	36.802536613132425	19.646325258100784
13	22.664566184237163	22.70084360024292	37.19717877951549	17.43741143600443
14	19.789029789570943	27.480042410504012	33.28623282083967	19.444694979085366
15	16.79043077928299	25.406016439080997	38.7436790110095	19.059873770626517
16	19.750146813299583	23.7464749776022	36.22650736672058	20.276870842377633
17	19.527872479872048	23.411560269615357	33.49026823240796	23.570299018104635
18	19.912292832905422	23.77273100797101	35.32398137625693	20.990994782866636
19	20.29551061966236	25.606043296394503	33.4704258888468	20.628020195096337
20	21.737988868244834	26.47770341909635	32.72142752634121	19.062880186317603
21	20.75749649752572	26.997212050515802	33.47944513592005	18.765846316038427
22	18.741193707371533	24.830388048094633	32.13156876775038	24.296849476783457
23	17.962932898806052	27.116666967308234	32.50376303030668	22.41663710357904
24	19.526870341308353	25.876821136304873	32.87375258802284	21.722555934363932
25	18.89752732330794	27.26778946271343	31.523671515013035	22.311011698965594
26	17.95952562768949	27.792308786951352	32.38190298096137	21.866262604397786
27	21.046914114720813	26.14058400626938	32.56048407301181	20.252017805997998
28	18.71213168902438	25.94075757666861	34.34629499351616	21.00081574079085
29	20.201910877813255	24.007431859588362	34.595426640450725	21.195230622147662
30	23.4662770351931	23.889981219923317	32.37729314356837	20.266448601315208
31	23.913230834601038	25.508435000290618	29.606780870377385	20.971553294730956
32	22.717879955825733	25.581190260014875	31.066696329968153	20.634233454191246
33	24.620941088282393	24.209462994029256	29.70899900387427	21.46059691381408
34	23.39191835376694	23.638644868148628	31.17933670452746	21.79010007355697
35	22.062080479743774	22.214004685999925	31.799660475454623	23.924254358801683
36	24.91396640430679	23.84628797854622	31.52507450900221	19.71467110814478
37	24.516718677658123	23.680333832398336	31.965414193889764	19.837533296053778
38	22.768788594861434	25.0857329541241	30.259974786193737	21.885503664820728
39	23.996809190813195	22.606843002968336	31.40622087534799	21.990126930870478
40	24.376619706453575	22.404611440814698	31.93555046469165	21.28321838804008
41	21.319696231758574	23.904412015240524	30.903748599511356	23.872143153489546
42	22.170912727761042	23.856710219608644	31.84676098794828	22.12561606468203
43	23.252420665700605	23.347824256964362	32.01932924861654	21.380425828718487
44	23.32637849170129	23.643855988679842	30.860255785846995	22.169509733771868
45	23.43861801083512	22.484782525910294	30.1132617004688	23.963337762785784
46	24.537964015208455	23.90721800321887	30.25456323794978	21.30025474362289
47	21.29684747250633	24.09261363750243	32.754698526655886	21.855840363335357
48	21.312681261812706	23.255026225966212	31.718086396369856	23.714206115851226
49	22.660758057695123	21.275201279530517	33.27240330866068	22.79163735411368
50	21.07958383189727	21.096620187480084	32.871547883182714	24.952248097439934
51	20.97275586100739	21.580853541457472	30.311885563793133	27.134505033742006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	261.0
1	507.0
2	753.0
3	5264.0
4	9775.0
5	6220.0
6	2665.0
7	2700.0
8	2735.0
9	2737.5
10	2740.0
11	2771.0
12	2802.0
13	2808.5
14	2815.0
15	2674.0
16	2533.0
17	2477.0
18	2421.0
19	2351.5
20	2282.0
21	2344.0
22	2406.0
23	2637.0
24	2868.0
25	3232.0
26	4247.0
27	4898.0
28	5718.0
29	6538.0
30	7160.0
31	7782.0
32	8887.0
33	9992.0
34	11794.5
35	13597.0
36	14318.5
37	15040.0
38	16139.5
39	17239.0
40	18875.5
41	20512.0
42	23007.5
43	25503.0
44	28288.0
45	31073.0
46	33275.5
47	35478.0
48	43618.0
49	51758.0
50	54649.0
51	57540.0
52	54914.5
53	52289.0
54	42923.0
55	33557.0
56	27276.5
57	20996.0
58	18200.5
59	15405.0
60	14046.5
61	12688.0
62	11190.5
63	9693.0
64	7834.0
65	5975.0
66	4756.5
67	3538.0
68	2874.0
69	2210.0
70	1943.0
71	1676.0
72	1556.5
73	1437.0
74	1186.0
75	671.5
76	408.0
77	308.0
78	208.0
79	188.5
80	169.0
81	124.5
82	80.0
83	63.5
84	47.0
85	30.0
86	13.0
87	7.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.830309952912984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59330693540691	27.84057611826305
2	10.185047337647742	7.7060699533661055
3	3.7038473757898065	4.203530827332353
4	2.110630389464014	3.1938320731784438
5	1.4177934643271943	2.681778310235602
6	1.0694269844388162	2.427405257599768
7	0.8614646858341719	2.281263325501687
8	0.7649431067364716	2.3150427859347937
9	0.6470061496362439	2.2028798863961914
>10	5.5258978595254815	36.70500276586707
>50	0.07652661855421589	2.000181297560112
>100	0.038263309277105785	2.7022250297019124
>500	0.0015943045532127408	0.4290267251978186
>1k	0.0037200439574963957	1.9718341709185592
>5k	5.314348510709137E-4	1.3393514729465172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6662	1.3352494222671178	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2145	0.4299174438251228	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1686	0.3379211236779287	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	1484	0.29743472570465374	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	1224	0.24532352039251765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	1135	0.22748545395874795	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	1106	0.2216730502893174	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	1028	0.20603968869567657	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	846	0.1695618449771813	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	715	0.14330581460837427	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	573	0.1148450793994384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.00427712738985E-4	0.0	0.0	0.307255683628864	0.0
2	2.00427712738985E-4	0.0	0.0	1.3562943321047114	0.0
3	2.00427712738985E-4	0.0	0.0	1.869589704429252	0.0
4	2.00427712738985E-4	0.0	0.0	2.565474723059008	0.0
5	2.00427712738985E-4	0.0	0.0	4.405601553715629	0.0
6	2.00427712738985E-4	0.0	0.0	5.533207865585159	0.0
7	2.00427712738985E-4	0.0	0.0	6.645782098999264	0.0
8	2.00427712738985E-4	0.0	0.0	8.36665444057619	0.0
9	2.00427712738985E-4	0.0	0.0	9.195222605039154	0.0
10	2.00427712738985E-4	0.0	0.0	11.013302387294486	0.0
11	2.00427712738985E-4	0.0	0.0	13.29316761970044	0.0
12	2.00427712738985E-4	0.0	0.0	15.249342096032935	0.0
13	2.00427712738985E-4	0.0	0.0	15.955849783437856	0.0
14	2.00427712738985E-4	0.0	0.0	16.246269539196646	0.0
15	2.00427712738985E-4	0.0	0.0	16.7411255619492	0.0
16	2.00427712738985E-4	0.0	0.0	17.685340516662556	0.0
17	2.00427712738985E-4	0.0	0.0	18.88369781112895	0.0
18	2.00427712738985E-4	0.0	0.0	20.203313871802425	0.0
19	2.00427712738985E-4	0.0	0.0	20.991195210579377	0.0
20	4.0085542547797E-4	0.0	0.0	21.685075952081743	0.0
21	4.0085542547797E-4	0.0	0.0	22.414632826451648	0.0
22	4.0085542547797E-4	0.0	0.0	23.24720954516939	0.0
23	4.0085542547797E-4	0.0	0.0	24.08078840245083	0.0
24	4.0085542547797E-4	0.0	0.0	24.692493781730214	0.0
25	4.0085542547797E-4	0.0	0.0	25.222023798786612	0.0
26	4.0085542547797E-4	0.0	0.0	25.715075972124513	0.0
27	4.0085542547797E-4	0.0	0.0	26.20552258519681	0.0
28	4.0085542547797E-4	0.0	0.0	26.72242565635065	0.0
29	4.0085542547797E-4	0.0	0.0	27.22008766708155	0.0
30	4.0085542547797E-4	0.0	0.0	27.8187652450329	0.0
31	4.0085542547797E-4	0.0	0.0	28.347092695812865	0.0
32	4.0085542547797E-4	0.0	0.0	28.840144869150766	0.0
33	4.0085542547797E-4	0.0	0.0	29.32558078940459	0.0
34	4.0085542547797E-4	0.0	0.0	29.801596607159677	0.0
35	4.0085542547797E-4	0.0	0.0	30.308077437251093	0.0
36	4.0085542547797E-4	0.0	0.0	30.805739447981992	0.0
37	4.0085542547797E-4	0.0	0.0	31.3023993201492	0.0
38	4.0085542547797E-4	0.0	0.0	31.77981813189346	0.0
39	4.0085542547797E-4	0.0	0.0	32.23979973262943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	30	2.1630221E-6	45.000004	28
CGTATGG	35	1.2100827E-7	45.000004	2
AAACACG	35	1.2100827E-7	45.000004	40
CGCACCC	30	2.1630221E-6	45.000004	31
GCTACGA	70	0.0	45.000004	10
TGATCCG	20	7.029445E-4	45.0	1
GTTTCGC	20	7.029445E-4	45.0	39
ACGCATA	20	7.029445E-4	45.0	33
GCAACGA	20	7.029445E-4	45.0	11
TTATCCG	20	7.029445E-4	45.0	1
ACGGGCC	20	7.029445E-4	45.0	5
CGTTAGA	20	7.029445E-4	45.0	11
TGTTGCG	25	3.8876206E-5	45.0	1
TACGCGG	20	7.029445E-4	45.0	2
GCTAGCG	25	3.8876206E-5	45.0	1
GCGGCTA	20	7.029445E-4	45.0	24
CTTTCGT	20	7.029445E-4	45.0	20
ATAACGG	45	3.8380676E-10	45.0	2
CCAACGG	20	7.029445E-4	45.0	2
CGCCGTT	20	7.029445E-4	45.0	26
>>END_MODULE