##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591613
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.327886642112325	33.0	31.0	34.0	30.0	34.0
2	32.44599763696876	34.0	31.0	34.0	31.0	34.0
3	32.44831672055888	34.0	31.0	34.0	30.0	34.0
4	35.963244553449634	37.0	35.0	37.0	35.0	37.0
5	36.051837941356936	37.0	35.0	37.0	35.0	37.0
6	36.07518766490932	37.0	35.0	37.0	35.0	37.0
7	36.20852482957609	37.0	37.0	37.0	35.0	37.0
8	36.10382124801179	37.0	37.0	37.0	35.0	37.0
9	37.953469582311406	39.0	38.0	39.0	35.0	39.0
10	37.694136200522976	39.0	37.0	39.0	35.0	39.0
11	37.64565687366572	39.0	37.0	39.0	35.0	39.0
12	37.54617799135583	39.0	37.0	39.0	35.0	39.0
13	37.49702761771631	39.0	37.0	39.0	35.0	39.0
14	38.80506682577969	40.0	38.0	41.0	35.0	41.0
15	38.84599053773328	40.0	38.0	41.0	35.0	41.0
16	38.884297674324266	40.0	38.0	41.0	35.0	41.0
17	38.81411158983998	40.0	38.0	41.0	35.0	41.0
18	38.7333375027256	40.0	38.0	41.0	35.0	41.0
19	38.63316221922101	40.0	37.0	41.0	35.0	41.0
20	38.504167420256145	40.0	37.0	41.0	35.0	41.0
21	38.423746604621606	40.0	37.0	41.0	35.0	41.0
22	38.40583624768218	40.0	37.0	41.0	35.0	41.0
23	38.36195790153361	40.0	37.0	41.0	35.0	41.0
24	38.27910982348258	40.0	37.0	41.0	34.0	41.0
25	38.176715183743426	40.0	36.0	41.0	34.0	41.0
26	38.169222109723755	40.0	36.0	41.0	34.0	41.0
27	38.14686627913856	40.0	36.0	41.0	34.0	41.0
28	38.05510020908939	40.0	36.0	41.0	34.0	41.0
29	38.001779879752476	40.0	36.0	41.0	34.0	41.0
30	37.84583672096455	40.0	36.0	41.0	34.0	41.0
31	37.80137353303596	40.0	36.0	41.0	34.0	41.0
32	37.64554024336855	40.0	36.0	41.0	33.0	41.0
33	37.499691521315455	40.0	35.0	41.0	33.0	41.0
34	37.34725065203097	40.0	35.0	41.0	33.0	41.0
35	37.251290962166145	40.0	35.0	41.0	33.0	41.0
36	37.12660134243162	40.0	35.0	41.0	33.0	41.0
37	37.07699627966255	40.0	35.0	41.0	32.0	41.0
38	36.88461713992086	40.0	35.0	41.0	32.0	41.0
39	36.85530236827115	39.0	35.0	41.0	32.0	41.0
40	36.76637261182564	39.0	35.0	41.0	31.0	41.0
41	36.672899344672956	39.0	35.0	41.0	31.0	41.0
42	36.59078316399403	39.0	35.0	41.0	31.0	41.0
43	36.53652810198559	39.0	35.0	41.0	31.0	41.0
44	36.50918590362281	39.0	35.0	41.0	31.0	41.0
45	36.37936962169526	39.0	35.0	41.0	31.0	41.0
46	36.334608941994176	39.0	35.0	41.0	31.0	41.0
47	36.258462880295056	39.0	35.0	41.0	31.0	41.0
48	36.190536719105225	38.0	35.0	41.0	31.0	41.0
49	36.13464376205391	38.0	35.0	41.0	31.0	41.0
50	36.04335097437007	38.0	35.0	41.0	31.0	41.0
51	35.22716708388761	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	9.0
10	19.0
11	18.0
12	8.0
13	17.0
14	15.0
15	35.0
16	55.0
17	82.0
18	110.0
19	232.0
20	372.0
21	676.0
22	1041.0
23	1625.0
24	2396.0
25	3503.0
26	5038.0
27	6316.0
28	6526.0
29	6675.0
30	7142.0
31	8898.0
32	10916.0
33	15347.0
34	30869.0
35	45575.0
36	41865.0
37	62349.0
38	113458.0
39	220161.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.561945055297976	19.664882279463093	23.527035409972395	14.246137255266532
2	33.46072517000134	25.34528483992069	23.70738979704638	17.48660019303159
3	31.90159783507124	24.142809573150014	27.62329428190388	16.33229830987487
4	28.89507160931217	23.81370929982945	28.703899339602074	18.587319751256313
5	26.388196337808665	28.689362809809793	25.249952249189928	19.672488603191614
6	26.542858253621876	33.80774256143796	24.818082090826266	14.831317094113889
7	76.71214121393545	6.2567928696631085	12.256153938469911	4.774911977931519
8	76.65027644761018	7.217218012450707	10.835292665982662	5.297212873956455
9	70.39314551911468	7.993739150424347	13.314109054398738	8.299006276062224
10	39.36796520698497	25.206342659813085	19.510896481314642	15.914795651887298
11	30.753211981481137	23.654821648611506	25.1666207470086	20.425345622898753
12	26.458681604359608	21.64894956669309	30.662781243819865	21.22958758512744
13	24.277864076685265	22.361577585347177	32.48390417384338	20.876654164124183
14	21.616495918784747	26.17741665582061	30.21468426150203	21.991403163892613
15	21.027597432781228	23.342286258077493	33.96206641841879	21.668049890722482
16	23.383022347379114	22.537875266432618	31.23257940579399	22.84652298039428
17	23.288027815480728	23.17444004780152	29.11126023261828	24.42627190409947
18	23.952144391688485	22.966871924721058	30.143353847870145	22.937629835720312
19	24.909188946152298	24.41410178613384	27.819199375267278	22.857509892446583
20	25.464788637166524	24.75959791282477	28.94966811074131	20.825945339267392
21	25.28291298534684	25.545246639272634	27.944450172663547	21.22739020271698
22	22.936953718055552	23.413954730541757	28.024908174769653	25.624183376633034
23	23.000339749126542	24.736947971055404	28.376151301611017	23.88656097820704
24	23.274674491601775	24.428131227677554	28.874619049953264	23.42257523076741
25	23.843796535911142	25.600519258366532	27.27137503739776	23.28430916832456
26	23.032793397034887	24.819096267323403	28.235518827341522	23.912591508300192
27	24.513829141685527	24.12032866079684	28.129030295142265	23.236811902375372
28	21.92396042683308	25.76566099798348	29.037225348327368	23.27315322685607
29	23.727842356405286	24.453992728354514	27.518834102698893	24.299330812541307
30	23.534472704284724	24.628769144694253	27.70544257817188	24.131315572849143
31	24.977983918541344	25.359990399129163	25.25637536700512	24.405650315324376
32	24.779036295686538	25.54288022744598	25.648861671396674	24.029221805470808
33	23.469396379051847	25.579559610759063	25.322465868735133	25.628578141453957
34	23.22430372557736	25.84240035293342	27.11468476859028	23.81861115289894
35	24.919161681707465	23.904140037490723	26.16288012602833	25.013818154773475
36	24.593949084959256	27.060088267161138	26.27765109962087	22.06831154825874
37	22.71366585926949	27.17181671126226	26.89646779228989	23.21804963717836
38	22.5823300028904	28.130889618720346	25.900039383853972	23.38674099453528
39	24.11542680772735	24.154134544034697	27.913855848333284	23.81658279990467
40	23.339412758002275	24.179827015295473	29.390834886995382	23.08992533970687
41	22.069325724755878	24.75452703033909	28.222672591711135	24.953474653193897
42	22.793954831959407	24.586680820063116	28.312934299956222	24.30643004802126
43	23.790552269811517	23.076572015827914	28.278283269637416	24.854592444723156
44	23.33146837544138	23.782776916666805	27.680088165743484	25.20566654214833
45	23.030765044040614	23.577575205412998	27.403217982025414	25.988441768520975
46	24.198589280492484	24.74776585369152	27.409810129256794	23.643834736559203
47	22.423273322256275	24.299668871373687	29.846538193041734	23.430519613328308
48	22.44119044037234	24.59749870269923	28.022541762943003	24.938769093985428
49	22.452177352424645	23.67138653139806	30.24629276232943	23.63014335384787
50	21.681572244017627	22.96974542479628	29.96063304896951	25.388049282216585
51	22.476010500107332	23.3674716410897	27.303490626473724	26.85302723232924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	797.0
2	1362.0
3	4920.5
4	8479.0
5	5548.0
6	2617.0
7	2472.5
8	2328.0
9	2281.5
10	2235.0
11	2257.0
12	2279.0
13	2202.0
14	2125.0
15	2052.0
16	1979.0
17	1833.0
18	1687.0
19	1592.0
20	1497.0
21	1589.0
22	1681.0
23	1920.0
24	2159.0
25	2640.5
26	3618.0
27	4114.0
28	5310.0
29	6506.0
30	7381.0
31	8256.0
32	8642.5
33	9029.0
34	9621.5
35	10214.0
36	11205.5
37	12197.0
38	13703.0
39	15209.0
40	17381.0
41	19553.0
42	22724.5
43	25896.0
44	29352.5
45	32809.0
46	41411.5
47	50014.0
48	51688.0
49	53362.0
50	52692.5
51	52023.0
52	46559.0
53	41095.0
54	38461.0
55	35827.0
56	33789.0
57	31751.0
58	31181.5
59	30612.0
60	29362.5
61	28113.0
62	25738.5
63	23364.0
64	21111.0
65	18858.0
66	16726.0
67	14594.0
68	12522.0
69	10450.0
70	9283.0
71	8116.0
72	7196.0
73	6276.0
74	5100.5
75	3285.5
76	2646.0
77	2090.5
78	1535.0
79	1168.0
80	801.0
81	654.5
82	508.0
83	309.5
84	111.0
85	78.0
86	45.0
87	30.5
88	16.0
89	8.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	3.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.609567180627508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44599934237779	22.175406838382088
2	8.796562187483142	5.385179224726658
3	3.2721089798727254	3.004735189252462
4	1.7717998915915096	2.1693611124919543
5	1.2803155912686526	1.9594953051671324
6	1.0520664910157094	1.932197996113944
7	0.8709611605919076	1.8661820909798712
8	0.8130263668437385	1.9909108156419935
9	0.7379579689251401	2.0329716623664154
>10	8.804903948951495	49.15324608287423
>50	0.09602002264940075	2.0164479654864746
>100	0.051062671004304915	3.0536385316078456
>500	0.004440232261243905	0.9816358871497793
>1k	0.0022201161306219523	1.379692807120692
>5k	5.550290326554881E-4	0.8988984906385398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5291	0.8943346410575833	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	2170	0.36679383313077973	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	2096	0.3542856563327716	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	2085	0.3524263327546893	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1770	0.29918206665506	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCC	847	0.14316791551233662	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	842	0.1423227684313901	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	816	0.13792800361046834	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	794	0.13420935645430374	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	755	0.12761720922292108	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	607	0.10260085562690474	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3805883237859885E-4	0.0	0.0	0.1472246215008798	0.0
2	3.3805883237859885E-4	0.0	0.0	0.7367992251691562	0.0
3	3.3805883237859885E-4	0.0	0.0	0.9974425849330559	0.0
4	3.3805883237859885E-4	0.0	0.0	1.4230586548977118	0.0
5	3.3805883237859885E-4	0.0	0.0	2.7555175427179592	0.0
6	3.3805883237859885E-4	0.0	0.0	3.490964532557601	0.0
7	3.3805883237859885E-4	0.0	0.0	4.247878258253284	0.0
8	3.3805883237859885E-4	0.0	0.0	5.3957570235948165	0.0
9	3.3805883237859885E-4	0.0	0.0	5.887294565873299	0.0
10	5.070882485678983E-4	0.0	0.0	7.11765968631521	0.0
11	5.070882485678983E-4	0.0	0.0	8.259115333841548	0.0
12	5.070882485678983E-4	0.0	0.0	9.53004751416889	0.0
13	5.070882485678983E-4	0.0	0.0	9.904447671028189	0.0
14	5.070882485678983E-4	0.0	0.0	10.062659204581374	0.0
15	5.070882485678983E-4	0.0	0.0	10.40122512520854	0.0
16	5.070882485678983E-4	0.0	0.0	10.958008022136092	0.0
17	5.070882485678983E-4	0.0	0.0	11.609109333297274	0.0
18	5.070882485678983E-4	0.0	0.0	12.348951087957838	0.0
19	5.070882485678983E-4	0.0	0.0	12.837615130161103	0.0
20	5.070882485678983E-4	0.0	0.0	13.268809170860004	0.0
21	5.070882485678983E-4	0.0	0.0	13.772009742855548	0.0
22	5.070882485678983E-4	0.0	0.0	14.301409874360434	0.0
23	5.070882485678983E-4	0.0	0.0	14.821682417391099	0.0
24	5.070882485678983E-4	0.0	0.0	15.29175322381354	0.0
25	5.070882485678983E-4	0.0	0.0	15.694212263760262	0.0
26	5.070882485678983E-4	0.0	0.0	16.057456479151067	0.0
27	5.070882485678983E-4	0.0	0.0	16.422729047536144	0.0
28	5.070882485678983E-4	0.0	0.0	16.78817064533741	0.0
29	5.070882485678983E-4	0.0	0.0	17.184882685133694	0.0
30	5.070882485678983E-4	0.0	0.0	17.64227628534194	0.0
31	5.070882485678983E-4	0.0	0.0	18.043214060542958	0.0
32	5.070882485678983E-4	0.0	0.0	18.453617483050575	0.0
33	5.070882485678983E-4	0.0	0.0	18.861823523147734	0.0
34	5.070882485678983E-4	0.0	0.0	19.263944504262078	0.0
35	5.070882485678983E-4	0.0	0.0	19.67502404443445	0.0
36	6.761176647571977E-4	0.0	0.0	20.077483084381175	0.0
37	6.761176647571977E-4	0.0	0.0	20.460503741466127	0.0
38	6.761176647571977E-4	0.0	0.0	20.851975869360544	0.0
39	6.761176647571977E-4	0.0	0.0	21.26271735070054	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	30	2.163708E-6	45.000004	2
ATTAGCG	25	3.888502E-5	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
ACGTTAG	25	3.888502E-5	45.0	1
CGCATGT	20	7.0305075E-4	45.0	38
TTATGCG	25	3.888502E-5	45.0	1
TAACGAG	20	7.0305075E-4	45.0	1
TATTGCG	20	7.0305075E-4	45.0	1
TAACACG	25	3.888502E-5	45.0	1
CGTGTAC	80	0.0	45.0	18
TATAGCG	20	7.0305075E-4	45.0	1
TTACCGG	40	6.8048394E-9	45.0	39
GATTACG	25	3.888502E-5	45.0	1
ATCGCAT	20	7.0305075E-4	45.0	10
GCGTAAG	35	1.210592E-7	45.0	1
CCCTCGT	85	0.0	42.35294	14
AACACGG	75	0.0	42.000004	2
CGTTTTT	2355	0.0	41.17834	1
CGAAAAT	50	1.0804797E-9	40.5	23
TGCGATG	50	1.0804797E-9	40.5	1
>>END_MODULE