FastQCFastQC Report
Sat 18 Jun 2016
SRR3551569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434546
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.5985557340304594No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC16420.3778656344782831TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG14310.3293092100721213No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC13410.30859793899840293No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC12700.2922590473735807No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT7600.17489517795584358TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC6920.15924666203347862TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC5670.13048100776442542No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5640.12979063206196811No Hit
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC5400.12426762644230992TruSeq Adapter, Index 14 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4530.10424673107104887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG351.2095916E-745.0000041
CTCGTCA207.028439E-445.033
CGATTCT253.8867867E-545.010
TCGATTA207.028439E-445.041
CGTTCGG253.8867867E-545.045
TAGCACG207.028439E-445.01
ATATCAC800.045.030
AATTGCG453.8380676E-1045.01
TGATTCG253.8867867E-545.015
AGCGACT207.028439E-445.040
CGGTCTA253.8867867E-545.031
CGATCGA207.028439E-445.041
TATCGAG207.028439E-445.01
TGCGTAG207.028439E-445.01
TCGATGG302.1623746E-644.9999962
ATACGGG750.042.03
ATATTAG750.042.029
TAGTAAG556.002665E-1140.9090921
ATGCGGG1600.040.781253
AATGCGG501.0786607E-940.52
Produced by FastQC (version 0.11.5)