##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434546
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.318578470403594	33.0	31.0	34.0	30.0	34.0
2	32.435465980586635	34.0	31.0	34.0	31.0	34.0
3	32.44859692644737	34.0	31.0	34.0	30.0	34.0
4	35.959224109760534	37.0	35.0	37.0	35.0	37.0
5	36.04205308528901	37.0	35.0	37.0	35.0	37.0
6	36.064761843395175	37.0	35.0	37.0	35.0	37.0
7	36.1948217219811	37.0	37.0	37.0	35.0	37.0
8	36.09412812452537	37.0	37.0	37.0	35.0	37.0
9	37.94186346209607	39.0	38.0	39.0	35.0	39.0
10	37.67822509009403	39.0	37.0	39.0	35.0	39.0
11	37.59115260524778	39.0	37.0	39.0	35.0	39.0
12	37.485246671238485	39.0	37.0	39.0	35.0	39.0
13	37.423837752504916	39.0	37.0	39.0	35.0	39.0
14	38.69045164378455	40.0	38.0	41.0	35.0	41.0
15	38.74751809934967	40.0	38.0	41.0	35.0	41.0
16	38.79758874779655	40.0	38.0	41.0	35.0	41.0
17	38.71247002618825	40.0	38.0	41.0	35.0	41.0
18	38.64977240614342	40.0	38.0	41.0	35.0	41.0
19	38.57809069695728	40.0	37.0	41.0	35.0	41.0
20	38.48218370437192	40.0	37.0	41.0	35.0	41.0
21	38.40049845125717	40.0	37.0	41.0	34.0	41.0
22	38.36726146368854	40.0	37.0	41.0	34.0	41.0
23	38.31486655037671	40.0	37.0	41.0	34.0	41.0
24	38.23959028503312	40.0	37.0	41.0	34.0	41.0
25	38.128789587293404	40.0	36.0	41.0	34.0	41.0
26	38.14192743691117	40.0	36.0	41.0	34.0	41.0
27	38.112450695668585	40.0	36.0	41.0	34.0	41.0
28	38.03517924454488	40.0	36.0	41.0	34.0	41.0
29	37.9863006448109	40.0	36.0	41.0	34.0	41.0
30	37.82724498672177	40.0	36.0	41.0	34.0	41.0
31	37.79199900585899	40.0	36.0	41.0	34.0	41.0
32	37.60569882129855	40.0	36.0	41.0	33.0	41.0
33	37.494099588996335	40.0	35.0	41.0	33.0	41.0
34	37.361358751432526	40.0	35.0	41.0	33.0	41.0
35	37.220954743571454	40.0	35.0	41.0	33.0	41.0
36	37.17001422173947	40.0	35.0	41.0	33.0	41.0
37	37.11836951669098	40.0	35.0	41.0	33.0	41.0
38	36.91190575911411	39.0	35.0	41.0	32.0	41.0
39	36.909151160061306	39.0	35.0	41.0	32.0	41.0
40	36.81115923285452	39.0	35.0	41.0	32.0	41.0
41	36.714522743276895	39.0	35.0	41.0	31.0	41.0
42	36.64195965444395	39.0	35.0	41.0	31.0	41.0
43	36.65587992985783	39.0	35.0	41.0	31.0	41.0
44	36.63725819591021	39.0	35.0	41.0	32.0	41.0
45	36.525836160038295	39.0	35.0	41.0	31.0	41.0
46	36.46991112563457	39.0	35.0	41.0	31.0	41.0
47	36.38354282400482	39.0	35.0	41.0	31.0	41.0
48	36.324876998062344	38.0	35.0	41.0	31.0	41.0
49	36.28415863913141	38.0	35.0	41.0	31.0	41.0
50	36.21011584504287	38.0	35.0	41.0	31.0	41.0
51	35.37563111845466	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	9.0
11	12.0
12	12.0
13	17.0
14	22.0
15	30.0
16	35.0
17	63.0
18	104.0
19	183.0
20	318.0
21	521.0
22	854.0
23	1222.0
24	1790.0
25	2431.0
26	3429.0
27	4077.0
28	4373.0
29	4533.0
30	5192.0
31	6360.0
32	8301.0
33	11579.0
34	23703.0
35	35976.0
36	29352.0
37	45373.0
38	83933.0
39	160524.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.063901175019446	20.54097840044552	24.048317094162645	13.346803330372389
2	32.36297192932393	25.422164742052626	24.90346246427306	17.31140086435038
3	31.737491542897644	24.303986229305988	26.962162809000656	16.99635941879571
4	28.725612478310698	24.82015712950988	27.779797765944224	18.674432626235195
5	25.478315299185816	28.984503366732177	24.80703999116319	20.730141342918817
6	26.15971611751115	34.43893166661297	24.49406967271589	14.907282543159988
7	77.0790664279501	6.515765879791783	11.566324393735071	4.838843298523057
8	77.3354259387959	7.201769202800164	9.94785362194106	5.514951236462883
9	71.1317098765148	8.114906131916989	12.316992907540284	8.436391084027928
10	40.72204093467665	24.50120355497462	19.149641234759958	15.627114275588774
11	31.72552503072172	24.13990693735531	24.669885351608343	19.464682680314628
12	28.196784690228423	20.650287886667925	29.96943936890456	21.183488054199096
13	25.223796790213232	22.49704289074114	32.08981327638501	20.189347042660614
14	21.634993763606154	26.36751920395079	29.663602932715982	22.333884099727072
15	20.87857211894713	23.921287964910505	33.52372361038877	21.67641630575359
16	22.768820792275157	22.93082895711846	31.432115357177377	22.868234893429005
17	22.753632526821097	23.475535386357254	29.045256428548416	24.725575658273232
18	23.00446903204724	23.327334735563092	30.41012919230645	23.258067040083212
19	24.434697362304565	24.78793959672854	27.820529932389203	22.956833108577687
20	25.53446585631901	24.420429597787116	28.730445108227897	21.314659437665977
21	24.933608869947026	25.73007230534857	28.27226576703042	21.064053057673988
22	23.322962356114196	23.728903269159076	27.230949082490692	25.71718529223603
23	23.166937447358855	25.314005882000984	27.502726984024704	24.016329686615457
24	23.529844941617228	23.767564308496684	28.588687963990004	24.113902785896084
25	22.83854873822334	25.711892411850528	27.287329764858036	24.162229085068095
26	21.264722261854903	25.339779908226056	28.262370381961865	25.133127447957175
27	23.64398705775683	24.580596760757203	27.634358617959894	24.141057563526072
28	21.439617439810746	25.771034597027704	28.606637732253894	24.18271023090766
29	22.91057793651305	24.597856153318638	26.68578240278359	25.805783507384717
30	22.95015947678727	25.286851104371	26.900489246247812	24.862500172593926
31	25.080428769336272	26.271787106543382	23.948672867774643	24.699111256345706
32	25.36049117929978	26.00990459007792	25.081349270272884	23.54825496034942
33	24.208944507601036	26.159485992277	25.2583155753361	24.373253924785867
34	23.441476851702696	24.755261813478896	26.97182806883506	24.83143326598335
35	24.042563963308833	24.03658070722087	26.78174462542516	25.13911070404514
36	23.692773607397147	26.639297105484804	26.25061558500136	23.417313702116694
37	24.233567907655345	26.9803427024987	26.965614687512947	21.82047470233301
38	22.47518099349666	27.740906601372465	27.045238018529684	22.738674386601186
39	25.154989345201656	24.757102815352113	27.492371348487847	22.59553649095838
40	23.573338610872035	23.44838060872727	29.50941902583386	23.468861754566834
41	21.457337082840482	24.262103436690246	28.50883450773911	25.77172497273016
42	21.15242114758852	24.79806510703125	29.147432032512093	24.902081712868142
43	23.141393546367933	23.761120801940415	28.021199136570125	25.076286515121527
44	22.866624016789938	24.473588526876323	27.34278994628877	25.316997510044963
45	23.247941529780505	23.840514007723	26.72628444399442	26.18526001850207
46	23.902187570475853	24.838106897773766	27.369944723918753	23.88976080783162
47	21.77030740128778	24.072250118514496	30.216133619915958	23.941308860281765
48	21.815181821947505	23.696685736377738	28.815591444864296	25.67254099681047
49	22.63856990974488	23.30616321402107	30.207849111486475	23.847417764747576
50	21.84118597340673	22.781247554919386	30.095317871985934	25.28224859968795
51	22.024365659792057	23.128966783723705	27.3977898772512	27.448877679233043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	240.0
1	691.0
2	1142.0
3	2778.5
4	4415.0
5	2923.5
6	1432.0
7	1407.0
8	1382.0
9	1343.0
10	1304.0
11	1322.0
12	1340.0
13	1355.5
14	1371.0
15	1336.0
16	1301.0
17	1234.0
18	1167.0
19	1092.0
20	1017.0
21	1094.5
22	1172.0
23	1339.0
24	1506.0
25	1807.0
26	2687.0
27	3266.0
28	4200.0
29	5134.0
30	5599.0
31	6064.0
32	6555.5
33	7047.0
34	8075.5
35	9104.0
36	9842.5
37	10581.0
38	10877.5
39	11174.0
40	13377.0
41	15580.0
42	17683.0
43	19786.0
44	21778.5
45	23771.0
46	30281.0
47	36791.0
48	38073.0
49	39355.0
50	38308.0
51	37261.0
52	33798.5
53	30336.0
54	28419.0
55	26502.0
56	25103.5
57	23705.0
58	22849.5
59	21994.0
60	21546.0
61	21098.0
62	19205.5
63	17313.0
64	15929.5
65	14546.0
66	12508.5
67	10471.0
68	8975.5
69	7480.0
70	6456.0
71	5432.0
72	4823.0
73	4214.0
74	3427.5
75	2045.5
76	1450.0
77	1137.5
78	825.0
79	613.5
80	402.0
81	301.5
82	201.0
83	133.0
84	65.0
85	45.5
86	26.0
87	29.0
88	32.0
89	17.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.23522483391689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2960532047958	22.16122648085736
2	8.976658345925518	5.428225666926291
3	3.095512542487761	2.8078055309498153
4	1.7267506407456563	2.0883477542021986
5	1.2003254004271044	1.8146054177887412
6	0.8921835128645994	1.6185221462744968
7	0.7793315295730976	1.6494284811762068
8	0.6730034536259523	1.6278728587506583
9	0.6546320717464438	1.7813653085471886
>10	8.534447989706576	51.27190858643104
>50	0.11686830419431818	2.325669431010928
>100	0.046594552652636664	2.790341416630986
>500	0.0038192256272653	0.7212577589917702
>1k	0.0038192256272653	1.9134231614623174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2601	0.5985557340304594	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1642	0.3778656344782831	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	1431	0.3293092100721213	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	1341	0.30859793899840293	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	1270	0.2922590473735807	No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	760	0.17489517795584358	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	692	0.15924666203347862	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	567	0.13048100776442542	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	564	0.12979063206196811	No Hit
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	540	0.12426762644230992	TruSeq Adapter, Index 14 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	453	0.10424673107104887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.602504683048515E-4	0.0	0.0	0.1813386845121115	0.0
2	4.602504683048515E-4	0.0	0.0	0.9580113497765484	0.0
3	4.602504683048515E-4	0.0	0.0	1.3071113299857782	0.0
4	4.602504683048515E-4	0.0	0.0	1.7797885609348607	0.0
5	4.602504683048515E-4	0.0	0.0	3.2321089136708196	0.0
6	4.602504683048515E-4	0.0	0.0	3.9742627938123927	0.0
7	4.602504683048515E-4	0.0	0.0	4.742650950647342	0.0
8	4.602504683048515E-4	0.0	0.0	5.914908893419799	0.0
9	4.602504683048515E-4	0.0	0.0	6.428548416048013	0.0
10	4.602504683048515E-4	0.0	0.0	7.661559420636711	0.0
11	4.602504683048515E-4	0.0	0.0	8.864193894317287	0.0
12	4.602504683048515E-4	0.0	0.0	10.089840891413107	0.0
13	4.602504683048515E-4	0.0	0.0	10.481974290408841	0.0
14	4.602504683048515E-4	0.0	0.0	10.644672830954606	0.0
15	4.602504683048515E-4	0.0	0.0	10.982956925158671	0.0
16	4.602504683048515E-4	0.0	0.0	11.58427416199896	0.0
17	4.602504683048515E-4	0.0	0.0	12.296972012169022	0.0
18	4.602504683048515E-4	0.0	0.0	13.103560957873274	0.0
19	4.602504683048515E-4	0.0	0.0	13.63630087493614	0.0
20	4.602504683048515E-4	0.0	0.0	14.090568087153029	0.0
21	4.602504683048515E-4	0.0	0.0	14.597764103224975	0.0
22	4.602504683048515E-4	0.0	0.0	15.153056293234778	0.0
23	4.602504683048515E-4	0.0	0.0	15.696151845834503	0.0
24	4.602504683048515E-4	0.0	0.0	16.171820704827567	0.0
25	4.602504683048515E-4	0.0	0.0	16.5639541038233	0.0
26	4.602504683048515E-4	0.0	0.0	16.951484998135985	0.0
27	4.602504683048515E-4	0.0	0.0	17.33809539151206	0.0
28	4.602504683048515E-4	0.0	0.0	17.71849240356602	0.0
29	4.602504683048515E-4	0.0	0.0	18.088994030551426	0.0
30	4.602504683048515E-4	0.0	0.0	18.520248719353074	0.0
31	4.602504683048515E-4	0.0	0.0	18.899034854767965	0.0
32	4.602504683048515E-4	0.0	0.0	19.309118022027587	0.0
33	4.602504683048515E-4	0.0	0.0	19.70700455187713	0.0
34	4.602504683048515E-4	0.0	0.0	20.106501958365744	0.0
35	4.602504683048515E-4	0.0	0.0	20.51612487515706	0.0
36	4.602504683048515E-4	0.0	0.0	20.911019776962622	0.0
37	4.602504683048515E-4	0.0	0.0	21.29118666378243	0.0
38	4.602504683048515E-4	0.0	0.0	21.6817091861391	0.0
39	4.602504683048515E-4	0.0	0.0	22.054512065466028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	35	1.2095916E-7	45.000004	1
CTCGTCA	20	7.028439E-4	45.0	33
CGATTCT	25	3.8867867E-5	45.0	10
TCGATTA	20	7.028439E-4	45.0	41
CGTTCGG	25	3.8867867E-5	45.0	45
TAGCACG	20	7.028439E-4	45.0	1
ATATCAC	80	0.0	45.0	30
AATTGCG	45	3.8380676E-10	45.0	1
TGATTCG	25	3.8867867E-5	45.0	15
AGCGACT	20	7.028439E-4	45.0	40
CGGTCTA	25	3.8867867E-5	45.0	31
CGATCGA	20	7.028439E-4	45.0	41
TATCGAG	20	7.028439E-4	45.0	1
TGCGTAG	20	7.028439E-4	45.0	1
TCGATGG	30	2.1623746E-6	44.999996	2
ATACGGG	75	0.0	42.0	3
ATATTAG	75	0.0	42.0	29
TAGTAAG	55	6.002665E-11	40.909092	1
ATGCGGG	160	0.0	40.78125	3
AATGCGG	50	1.0786607E-9	40.5	2
>>END_MODULE