##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313605
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.383941582564056	33.0	31.0	34.0	31.0	34.0
2	32.504858022034085	34.0	31.0	34.0	31.0	34.0
3	32.49495384321041	34.0	31.0	34.0	31.0	34.0
4	36.00611597391623	37.0	35.0	37.0	35.0	37.0
5	36.0822340205035	37.0	35.0	37.0	35.0	37.0
6	36.098075604661915	37.0	36.0	37.0	35.0	37.0
7	36.20682705951754	37.0	37.0	37.0	35.0	37.0
8	36.135402815643886	37.0	37.0	37.0	35.0	37.0
9	37.96115176735065	39.0	38.0	39.0	35.0	39.0
10	37.70968575118381	39.0	37.0	39.0	35.0	39.0
11	37.66939302625915	39.0	37.0	39.0	35.0	39.0
12	37.58496197445832	39.0	37.0	39.0	35.0	39.0
13	37.5583903317868	39.0	37.0	39.0	35.0	39.0
14	38.86345243219974	40.0	38.0	41.0	35.0	41.0
15	38.93566110234212	40.0	38.0	41.0	35.0	41.0
16	38.96531943049377	40.0	38.0	41.0	35.0	41.0
17	38.915371247269654	40.0	38.0	41.0	35.0	41.0
18	38.83687760080356	40.0	38.0	41.0	35.0	41.0
19	38.742915450965384	40.0	38.0	41.0	35.0	41.0
20	38.647030500151466	40.0	38.0	41.0	35.0	41.0
21	38.53525613430908	40.0	38.0	41.0	35.0	41.0
22	38.493263819135535	40.0	37.0	41.0	35.0	41.0
23	38.45796463704342	40.0	37.0	41.0	35.0	41.0
24	38.37502271966327	40.0	37.0	41.0	35.0	41.0
25	38.239508298655956	40.0	37.0	41.0	34.0	41.0
26	38.24441255719775	40.0	37.0	41.0	34.0	41.0
27	38.22970934774637	40.0	37.0	41.0	34.0	41.0
28	38.151371948789084	40.0	37.0	41.0	34.0	41.0
29	38.08545144369509	40.0	36.0	41.0	34.0	41.0
30	37.92658599193253	40.0	36.0	41.0	34.0	41.0
31	37.900916758342504	40.0	36.0	41.0	34.0	41.0
32	37.73380526458443	40.0	36.0	41.0	33.0	41.0
33	37.626428150061386	40.0	36.0	41.0	33.0	41.0
34	37.48927791329858	40.0	36.0	41.0	33.0	41.0
35	37.380523269718275	40.0	35.0	41.0	33.0	41.0
36	37.275037068924284	40.0	35.0	41.0	33.0	41.0
37	37.2012882447665	40.0	35.0	41.0	33.0	41.0
38	37.05946652636278	40.0	35.0	41.0	32.0	41.0
39	37.01063120804834	40.0	35.0	41.0	32.0	41.0
40	36.901991358556145	39.0	35.0	41.0	32.0	41.0
41	36.814591604087944	39.0	35.0	41.0	32.0	41.0
42	36.72467275713078	39.0	35.0	41.0	31.0	41.0
43	36.66986495751024	39.0	35.0	41.0	31.0	41.0
44	36.623376540552606	39.0	35.0	41.0	31.0	41.0
45	36.51334321837981	39.0	35.0	41.0	31.0	41.0
46	36.41541110632802	39.0	35.0	41.0	31.0	41.0
47	36.360198338674444	39.0	35.0	41.0	31.0	41.0
48	36.291599304858025	39.0	35.0	41.0	31.0	41.0
49	36.21789512284562	38.0	35.0	41.0	31.0	41.0
50	36.131984502798105	38.0	35.0	41.0	31.0	41.0
51	35.248892715358494	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	11.0
10	10.0
11	6.0
12	11.0
13	13.0
14	12.0
15	20.0
16	29.0
17	49.0
18	70.0
19	136.0
20	211.0
21	331.0
22	446.0
23	779.0
24	1271.0
25	1819.0
26	2725.0
27	3149.0
28	3445.0
29	3352.0
30	3816.0
31	4524.0
32	5416.0
33	7678.0
34	15364.0
35	23271.0
36	21559.0
37	32499.0
38	58656.0
39	122774.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58065081870506	19.60332265110569	24.506943447967984	14.309083082221266
2	34.74019865754692	24.491956442021014	23.492929003045234	17.27491589738684
3	32.3575835844454	23.577111334321838	27.104478563798406	16.960826517434352
4	29.434479679852043	25.144688381881668	26.623300011160538	18.797531927105755
5	24.881618596642273	29.826373941742002	24.789783326158705	20.502224135457023
6	26.543900766888285	34.057811578259276	24.856108799285725	14.542178855566714
7	77.56508984231758	6.9501442897912975	10.650659268825434	4.834106599065704
8	77.60335453835239	6.317182442881969	10.745045519044657	5.3344174997209866
9	70.51800832257139	8.76771735144529	13.21248066835669	7.5017936576266315
10	38.38586757226447	25.63256325632563	20.24935826916025	15.732210902249646
11	30.16597311905104	24.234307488719885	25.433586836944567	20.166132555284513
12	26.477256421294303	21.691937309672994	31.03234961177277	20.79845665725993
13	24.48430350281405	23.238149901946716	32.09100620206948	20.186540393169754
14	21.362223178839624	26.269351572838445	30.114315779404027	22.254109468917907
15	20.25828669823504	24.511088790038425	33.59448988377098	21.63613462795555
16	22.56501012420083	23.609636325951435	31.622263675642927	22.203089874204814
17	22.968702667368184	23.556065751502686	28.517721337351126	24.957510243778
18	23.491015768243493	23.704341448637617	29.90513544108034	22.899507342038554
19	25.01682052263197	24.487492227483617	27.936735702555765	22.558951547328647
20	25.467706190908945	24.78595685655522	28.16759936863252	21.57873758390332
21	25.011399690693707	25.207825130339124	28.099679533170708	21.681095645796464
22	23.491653513177404	23.964541381674398	27.59299118317629	24.950813921971907
23	23.66480126273497	24.52065496404713	28.53876692017028	23.27577685304762
24	22.891854402831587	25.096219766904227	28.8149104765549	23.197015353709283
25	22.791409575740182	25.712600245531796	27.915690119736613	23.58030005899141
26	22.65333779754787	25.524146617560305	27.835334258063487	23.987181326828335
27	23.742606144672436	25.42497728033673	28.408666953651885	22.423749621338946
28	21.75411744072958	26.17974840962357	29.25399786355447	22.812136286092375
29	23.31978125348767	25.345259163597518	27.82066612458347	23.51429345833134
30	23.16034502000925	26.14913665279571	28.150699127883804	22.539819199311236
31	24.16830088805982	26.547727236491763	25.868528881873694	23.41544299357472
32	24.246743514931204	27.81396980277738	25.404569442451493	22.534717239839928
33	23.08540999027439	27.15900575564803	26.187401348830537	23.568182905247046
34	22.646960348208733	25.759474498174455	27.14848296423845	24.445082189378358
35	24.635130179684637	25.763619840244896	25.830901930772786	23.77034804929768
36	24.2489756221999	27.00530922657483	25.75628577350489	22.98942937772038
37	22.889941168029846	27.335979974809078	27.289743467100337	22.484335390060746
38	22.87431641714896	27.50912772436664	26.650723043318823	22.965832815165573
39	23.20562491031712	24.91446246073883	27.653258079431133	24.226654549512922
40	23.53852776582006	24.12270212528499	29.23741649527272	23.101353613622233
41	22.37623762376238	25.506927504344638	26.985858006090464	25.130976865802523
42	22.352959933674526	25.749270579231837	27.28623586996381	24.61153361712983
43	23.17437540855535	24.041070773744043	28.562044610258123	24.22250920744248
44	22.01176639403071	25.549656414916853	28.068430031408937	24.3701471596435
45	23.397586135425136	24.719950255895153	27.027949171728764	24.854514436950943
46	23.803510785861196	25.019690374834585	27.66760734044419	23.50919149886003
47	22.42438736627286	25.008529838491096	28.890164378756715	23.676918416479328
48	22.260168045790085	24.46421453739577	28.852218555188852	24.42339886162529
49	22.336059692925815	24.592720141579374	29.394301749015483	23.676918416479328
50	21.565982685225045	24.090177133655395	29.602844342405255	24.740995838714305
51	21.907495097335822	24.226335677045967	27.50912772436664	26.357041501251576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	524.0
2	838.0
3	2158.5
4	3479.0
5	2389.0
6	1299.0
7	1275.0
8	1251.0
9	1176.5
10	1102.0
11	1131.0
12	1160.0
13	1201.0
14	1242.0
15	1163.0
16	1084.0
17	1019.0
18	954.0
19	910.5
20	867.0
21	989.5
22	1112.0
23	1288.5
24	1465.0
25	1709.5
26	2410.0
27	2866.0
28	3401.0
29	3936.0
30	4567.0
31	5198.0
32	5692.0
33	6186.0
34	6431.5
35	6677.0
36	6955.0
37	7233.0
38	8396.5
39	9560.0
40	10443.0
41	11326.0
42	12897.5
43	14469.0
44	16321.5
45	18174.0
46	20931.5
47	23689.0
48	26147.5
49	28606.0
50	27553.5
51	26501.0
52	23868.5
53	21236.0
54	19333.5
55	17431.0
56	16557.5
57	15684.0
58	15127.5
59	14571.0
60	14426.5
61	14282.0
62	13124.0
63	11966.0
64	11289.5
65	10613.0
66	9081.0
67	7549.0
68	6338.5
69	5128.0
70	4700.5
71	4273.0
72	3718.0
73	3163.0
74	2735.0
75	1794.5
76	1282.0
77	1066.5
78	851.0
79	617.0
80	383.0
81	311.0
82	239.0
83	179.5
84	120.0
85	95.0
86	70.0
87	39.5
88	9.0
89	7.5
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	313605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.86428550154854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3273980088857	22.783954012435075
2	8.589168203309395	5.645537521087653
3	3.602985432686115	3.552286257531506
4	2.317737556089904	3.04683155044
5	1.825962002731028	3.0004468286365933
6	1.592197947391527	3.1395868750832827
7	1.462342349982185	3.364118554357396
8	1.3314904163346293	3.500678494799759
9	1.287540216717555	3.8082680349238265
>10	8.551304705381604	42.677484790746185
>50	0.06592525528897293	1.5057775560475992
>100	0.04295130268827024	2.818203688395169
>500	0.001997735008756756	0.45334179308954325
>1k	9.98867504378378E-4	0.7034840424264237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2143	0.6833436966885095	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	721	0.22990704867588205	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	660	0.21045582819151482	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC	499	0.15911736101146345	No Hit
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	487	0.15529089140798139	Illumina Single End Adapter 1 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	427	0.13615854339057093	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	386	0.12308477224534048	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	355	0.1131997257696784	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	334	0.10650340396358476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.377449339136812E-4	0.0	0.0	0.16007397841233398	0.0
2	6.377449339136812E-4	0.0	0.0	0.744248337877266	0.0
3	6.377449339136812E-4	0.0	0.0	1.0685416367723728	0.0
4	6.377449339136812E-4	0.0	0.0	1.46043589866233	0.0
5	6.377449339136812E-4	0.0	0.0	2.621450550852187	0.0
6	6.377449339136812E-4	0.0	0.0	3.3185057636198403	0.0
7	6.377449339136812E-4	0.0	0.0	4.009183527048357	0.0
8	6.377449339136812E-4	0.0	0.0	5.060506050605061	0.0
9	6.377449339136812E-4	0.0	0.0	5.515855933419429	0.0
10	6.377449339136812E-4	0.0	0.0	6.399451539356834	0.0
11	6.377449339136812E-4	0.0	0.0	7.6261539197398	0.0
12	6.377449339136812E-4	0.0	0.0	8.601903668627733	0.0
13	6.377449339136812E-4	0.0	0.0	8.98869597104638	0.0
14	6.377449339136812E-4	0.0	0.0	9.150683184260455	0.0
15	6.377449339136812E-4	0.0	0.0	9.461902712010332	0.0
16	6.377449339136812E-4	0.0	0.0	10.108576074998805	0.0
17	6.377449339136812E-4	0.0	0.0	10.76290237719424	0.0
18	6.377449339136812E-4	0.0	0.0	11.502367628067155	0.0
19	6.377449339136812E-4	0.0	0.0	11.906697916168428	0.0
20	6.377449339136812E-4	0.0	0.0	12.322507613080148	0.0
21	6.377449339136812E-4	0.0	0.0	12.804005038184977	0.0
22	6.377449339136812E-4	0.0	0.0	13.284864718355893	0.0
23	6.377449339136812E-4	0.0	0.0	13.773377337733773	0.0
24	6.377449339136812E-4	0.0	0.0	14.16527159962373	0.0
25	6.377449339136812E-4	0.0	0.0	14.513799206007556	0.0
26	6.377449339136812E-4	0.0	0.0	14.87093636899922	0.0
27	6.377449339136812E-4	0.0	0.0	15.201925989700419	0.0
28	6.377449339136812E-4	0.0	0.0	15.541525167009455	0.0
29	6.377449339136812E-4	0.0	0.0	15.878892237049792	0.0
30	6.377449339136812E-4	0.0	0.0	16.274931841010186	0.0
31	6.377449339136812E-4	0.0	0.0	16.645461647614038	0.0
32	6.377449339136812E-4	0.0	0.0	17.00961400487875	0.0
33	6.377449339136812E-4	0.0	0.0	17.37280974474259	0.0
34	6.377449339136812E-4	0.0	0.0	17.696146426236826	0.0
35	6.377449339136812E-4	0.0	0.0	18.069864957510244	0.0
36	6.377449339136812E-4	0.0	0.0	18.397346981074918	0.0
37	6.377449339136812E-4	0.0	0.0	18.735032923582214	0.0
38	6.377449339136812E-4	0.0	0.0	19.078777442961687	0.0
39	6.377449339136812E-4	0.0	0.0	19.419333237671594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	55	1.8189894E-12	45.000004	12
GCTACGA	55	1.8189894E-12	45.000004	10
TCGTTTA	20	7.025434E-4	45.0	38
CCGGGTT	20	7.025434E-4	45.0	5
AGATACG	40	6.7884685E-9	45.0	1
ATAGCGG	20	7.025434E-4	45.0	2
TACGGGA	80	0.0	45.0	4
TCTAGCG	20	7.025434E-4	45.0	1
TATTAGT	20	7.025434E-4	45.0	30
AACGATG	20	7.025434E-4	45.0	1
TAGGGTC	40	6.7884685E-9	45.0	5
TGTTACG	20	7.025434E-4	45.0	1
CATCGTT	20	7.025434E-4	45.0	36
CGTAAAC	20	7.025434E-4	45.0	38
GCGTAAG	20	7.025434E-4	45.0	1
GCATCGC	20	7.025434E-4	45.0	22
CTCGTAC	30	2.1604355E-6	44.999996	29
CCGATAA	30	2.1604355E-6	44.999996	11
TAGCATA	60	0.0	44.999996	30
TCGTACG	30	2.1604355E-6	44.999996	30
>>END_MODULE