FastQCFastQC Report
Sat 18 Jun 2016
SRR3551567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367345
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.6424478351413522No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC8900.24227905647279807No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC7180.19545658713198766No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC5860.15952306414950523No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT5370.14618410486055342No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC4410.12005063360056621No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4310.11732839701098423No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3990.10861723992432182No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3810.10371721406307421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT207.027011E-445.00000437
GCGGTAT207.027011E-445.0000046
CGTTATC207.027011E-445.00000445
ACGGCTA207.027011E-445.00000434
CGGCTAT207.027011E-445.00000435
ACGTAGG406.7939254E-945.0000042
CGGTATG207.027011E-445.0000047
CGAAAGG551.8189894E-1245.02
ACGGGTC253.885606E-545.05
AGTAGCG253.885606E-545.01
TACGTAG253.885606E-545.01
ACGGTCT351.2089185E-745.030
CGATTGT302.1614542E-644.99999610
CACGACG302.1614542E-644.99999626
TAGGGCG1450.043.4482735
GCGATCA650.041.538469
AGCGACT556.002665E-1140.90909219
TAGGGTC501.0768417E-940.55
CGTTTTT11200.040.3794631
CGTGCGG451.9221261E-840.0000042