##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551567_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 367345 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.375611482393936 33.0 31.0 34.0 31.0 34.0 2 32.49281193428521 34.0 31.0 34.0 31.0 34.0 3 32.49057697804516 34.0 31.0 34.0 31.0 34.0 4 36.002360179123166 37.0 35.0 37.0 35.0 37.0 5 36.09092542432863 37.0 35.0 37.0 35.0 37.0 6 36.11359893288326 37.0 36.0 37.0 35.0 37.0 7 36.2589772557133 37.0 37.0 37.0 35.0 37.0 8 36.201840231934554 37.0 37.0 37.0 35.0 37.0 9 38.04961548408172 39.0 39.0 39.0 35.0 39.0 10 37.73409737440281 39.0 38.0 39.0 35.0 39.0 11 37.66375205869142 39.0 37.0 39.0 35.0 39.0 12 37.60568947447223 39.0 37.0 39.0 35.0 39.0 13 37.57711960146457 39.0 37.0 39.0 35.0 39.0 14 38.87757285385673 40.0 38.0 41.0 35.0 41.0 15 38.9562073799834 40.0 38.0 41.0 35.0 41.0 16 38.97989356054935 40.0 38.0 41.0 35.0 41.0 17 38.92205964420368 40.0 38.0 41.0 35.0 41.0 18 38.86751691189481 40.0 38.0 41.0 35.0 41.0 19 38.79068995086363 40.0 38.0 41.0 35.0 41.0 20 38.72024663463502 40.0 38.0 41.0 35.0 41.0 21 38.61476813349848 40.0 38.0 41.0 35.0 41.0 22 38.61913732322476 40.0 38.0 41.0 35.0 41.0 23 38.567785052198886 40.0 38.0 41.0 35.0 41.0 24 38.49643795342253 40.0 37.0 41.0 35.0 41.0 25 38.395805033415456 40.0 37.0 41.0 35.0 41.0 26 38.40656331241748 40.0 37.0 41.0 35.0 41.0 27 38.40442635669466 40.0 37.0 41.0 35.0 41.0 28 38.353468809974274 40.0 37.0 41.0 35.0 41.0 29 38.28909880357702 40.0 37.0 41.0 34.0 41.0 30 38.11202820237107 40.0 37.0 41.0 34.0 41.0 31 38.09835713021819 40.0 37.0 41.0 34.0 41.0 32 37.96909444799847 40.0 37.0 41.0 34.0 41.0 33 37.88004464468007 40.0 37.0 41.0 34.0 41.0 34 37.7565204371912 40.0 36.0 41.0 34.0 41.0 35 37.66147626890253 40.0 36.0 41.0 33.0 41.0 36 37.579109556411545 40.0 36.0 41.0 33.0 41.0 37 37.529110236970695 40.0 36.0 41.0 33.0 41.0 38 37.37140018238985 40.0 35.0 41.0 33.0 41.0 39 37.38348963508418 40.0 35.0 41.0 33.0 41.0 40 37.327237338197065 40.0 35.0 41.0 33.0 41.0 41 37.248055642515894 40.0 35.0 41.0 33.0 41.0 42 37.17690726701058 40.0 35.0 41.0 33.0 41.0 43 37.150806462589664 40.0 35.0 41.0 33.0 41.0 44 37.09393349576012 40.0 35.0 41.0 33.0 41.0 45 36.9659774871034 39.0 35.0 41.0 32.0 41.0 46 36.9134818767099 39.0 35.0 41.0 32.0 41.0 47 36.86739985572146 39.0 35.0 41.0 32.0 41.0 48 36.80143189644612 39.0 35.0 41.0 32.0 41.0 49 36.75604676802461 39.0 35.0 41.0 32.0 41.0 50 36.6733996651649 39.0 35.0 41.0 32.0 41.0 51 35.862358273557554 38.0 35.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 4.0 10 9.0 11 8.0 12 9.0 13 10.0 14 13.0 15 22.0 16 23.0 17 44.0 18 68.0 19 115.0 20 180.0 21 314.0 22 476.0 23 670.0 24 1086.0 25 1791.0 26 2523.0 27 3175.0 28 3445.0 29 3586.0 30 3904.0 31 4657.0 32 5879.0 33 8536.0 34 17581.0 35 27240.0 36 23251.0 37 35143.0 38 68920.0 39 154458.0 40 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.20552886251344 20.565408539656183 26.060787543045365 13.168275054785012 2 33.407831874668226 23.920020688998083 24.77316963617308 17.898977800160612 3 31.520233023452064 23.796703371490015 28.024336795110866 16.65872680994705 4 29.169853952006967 24.894309164409478 26.20397718765738 19.731859695926175 5 24.696947011664783 29.121670364371365 25.976942656086237 20.204439967877608 6 26.9482366712491 32.8459622425785 24.72008602267623 15.485715063496169 7 79.3180797343097 5.828036314636105 10.153398031822945 4.700485919231241 8 80.30842940559964 5.403095183002355 9.345438212035008 4.943037199362997 9 73.79820060161428 8.012903401434619 11.628033592399516 6.560862404551579 10 36.32307503845159 30.78985694646722 18.378363663586004 14.508704351495188 11 28.86441900665587 23.81902571152459 28.077148184948754 19.23940709687079 12 26.84234166791436 21.670908818685433 31.28584845308906 20.200901060311153 13 24.80964760647348 23.49045175516204 32.23209789162776 19.467802746736716 14 21.28843457784916 26.613129347071553 30.190420449441262 21.908015625638026 15 19.689392805128694 25.47006220310607 34.1009133103758 20.73963168138943 16 22.39284596224258 23.69162503913215 32.85467339966516 21.060855598960103 17 21.598225101743594 23.97147096054118 29.554778205773864 24.875525731941366 18 22.993915801222283 23.50869074031224 30.93467993303298 22.562713525432496 19 23.01406035198519 24.97543181477902 28.813513182430682 23.196994650805102 20 24.860008983380748 25.045665518790237 28.633028896541397 21.461296601287618 21 23.706052893056935 25.856892022485674 29.578733887762187 20.858321196695204 22 22.482679769698784 24.322911704256217 28.06898147518001 25.12542705086499 23 21.043977732104697 26.67002409179382 29.370210565000203 22.915787611101283 24 22.4200683281384 23.98671548544284 30.604744858375643 22.98847132804312 25 22.44375178646776 25.686480011977842 28.41824442962338 23.45152377193102 26 21.16783949693068 27.04623718847405 28.19774326586724 23.588180048728034 27 23.196722427146142 25.78066939797738 29.26649335093713 21.75611482393935 28 21.155861655936516 25.572690522533314 29.792429460044374 23.479018361485796 29 22.568974669588535 25.26888891913596 28.92267486967292 23.23946154160258 30 23.176305652724277 25.271338932066584 30.172998135267935 21.3793572799412 31 25.236766527378897 25.818780710231525 26.566851325048663 22.377601437340918 32 23.85686480011978 26.74842450557378 27.08979297390736 22.304917720399082 33 23.56204657746805 26.317494453442947 27.158937783282745 22.96152118580626 34 23.619485769508227 25.870503205433582 28.043936898555856 22.466074126502335 35 23.501612925179327 24.966448434033403 27.732785256366633 23.79915338442064 36 23.623569124392603 26.531190025725138 28.15309858579809 21.692142264084172 37 23.61295240169323 26.35206685813064 28.84127999564442 21.193700744531707 38 22.195211585838926 27.83105799725054 27.13715989056609 22.836570526344442 39 23.577563326028663 25.71125236494304 28.106003892798327 22.605180416229974 40 23.75478092801045 24.436156746382828 29.41131633750289 22.397745988103825 41 21.357579387224543 24.66863575113313 28.81922987926881 25.15455498237352 42 22.8433761178184 24.733697205624143 28.661340157073052 23.76158651948441 43 23.22884481890321 24.903020321496143 29.262954443370674 22.605180416229974 44 23.3513454654344 25.406361866909855 27.85555812655678 23.386734541098967 45 23.120772026296805 25.089765751541464 27.696851733384147 24.09261048877758 46 23.764853203391905 25.85634757516776 27.529706406783816 22.849092814656522 47 22.488396466536905 25.45236766527379 29.38300507697124 22.676230791218067 48 22.051749717567954 24.515373831139662 29.769562672691883 23.663313778600497 49 22.548013447848753 24.097510514638827 30.349943513590766 23.004532523921654 50 21.24950659461814 23.938804121466198 30.068736473886947 24.742952810028722 51 21.424274183669304 23.843525840830825 28.320788359716342 26.411411615783525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 195.0 1 480.0 2 765.0 3 2248.0 4 3731.0 5 2451.5 6 1172.0 7 1187.5 8 1203.0 9 1215.0 10 1227.0 11 1149.0 12 1071.0 13 1106.0 14 1141.0 15 1076.0 16 1011.0 17 1029.5 18 1048.0 19 1077.0 20 1106.0 21 1106.0 22 1106.0 23 1142.0 24 1178.0 25 1690.0 26 2618.5 27 3035.0 28 3641.5 29 4248.0 30 4855.5 31 5463.0 32 6004.0 33 6545.0 34 7752.0 35 8959.0 36 10005.5 37 11052.0 38 11396.5 39 11741.0 40 13576.5 41 15412.0 42 17213.0 43 19014.0 44 21084.0 45 23154.0 46 26989.0 47 30824.0 48 33332.0 49 35840.0 50 36007.5 51 36175.0 52 31303.5 53 26432.0 54 24335.5 55 22239.0 56 20085.5 57 17932.0 58 17046.5 59 16161.0 60 15032.0 61 13903.0 62 13154.0 63 12405.0 64 10971.0 65 9537.0 66 7962.0 67 6387.0 68 5225.0 69 4063.0 70 3453.5 71 2844.0 72 2681.0 73 2518.0 74 2005.5 75 1188.5 76 884.0 77 661.0 78 438.0 79 377.0 80 316.0 81 215.0 82 114.0 83 68.5 84 23.0 85 22.0 86 21.0 87 16.5 88 12.0 89 8.0 90 4.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 367345.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.709568434069276 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7599959124057 23.799480655594348 2 8.849446485030391 5.789231508114708 3 3.2224612067811256 3.162159461080221 4 1.8107074376948047 2.3690983538942576 5 1.307498775073785 2.138386033036886 6 1.0450626905901352 2.051012975745035 7 0.9265422195757131 2.121475729678636 8 0.8339543186540211 2.1822628685521055 9 0.7597289359910733 2.2365365060828384 >10 8.36618119552823 48.8684922190627 >50 0.08172704663488056 1.857094871444992 >100 0.03252402876286063 2.0360393831470947 >500 0.0033357978218318597 0.7449656424671377 >1k 8.339494554579649E-4 0.6437637920990278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2360 0.6424478351413522 No Hit GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 890 0.24227905647279807 No Hit CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 718 0.19545658713198766 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC 586 0.15952306414950523 No Hit CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT 537 0.14618410486055342 No Hit TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 441 0.12005063360056621 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 431 0.11732839701098423 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 399 0.10861723992432182 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 381 0.10371721406307421 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.7222365895820006E-4 0.0 0.0 0.15271747267555022 0.0 2 2.7222365895820006E-4 0.0 0.0 0.817759871510433 0.0 3 2.7222365895820006E-4 0.0 0.0 1.175733983040466 0.0 4 2.7222365895820006E-4 0.0 0.0 1.641236439858988 0.0 5 2.7222365895820006E-4 0.0 0.0 2.952810028719596 0.0 6 2.7222365895820006E-4 0.0 0.0 3.7346363772475466 0.0 7 2.7222365895820006E-4 0.0 0.0 4.498495964284256 0.0 8 2.7222365895820006E-4 0.0 0.0 5.723230205937198 0.0 9 2.7222365895820006E-4 0.0 0.0 6.248077420408608 0.0 10 2.7222365895820006E-4 0.0 0.0 7.290694034218514 0.0 11 2.7222365895820006E-4 0.0 0.0 8.66161238073201 0.0 12 2.7222365895820006E-4 0.0 0.0 9.739345846547524 0.0 13 2.7222365895820006E-4 0.0 0.0 10.150131347915448 0.0 14 2.7222365895820006E-4 0.0 0.0 10.311287754018702 0.0 15 2.7222365895820006E-4 0.0 0.0 10.630333882317712 0.0 16 2.7222365895820006E-4 0.0 0.0 11.247192693516993 0.0 17 2.7222365895820006E-4 0.0 0.0 12.009146714940995 0.0 18 2.7222365895820006E-4 0.0 0.0 12.855762294300998 0.0 19 2.7222365895820006E-4 0.0 0.0 13.308470239148484 0.0 20 2.7222365895820006E-4 0.0 0.0 13.757094829111598 0.0 21 5.444473179164001E-4 0.0 0.0 14.305081054594455 0.0 22 5.444473179164001E-4 0.0 0.0 14.89117859233146 0.0 23 5.444473179164001E-4 0.0 0.0 15.45822047394139 0.0 24 5.444473179164001E-4 0.0 0.0 15.921817365147206 0.0 25 5.444473179164001E-4 0.0 0.0 16.321441696497843 0.0 26 5.444473179164001E-4 0.0 0.0 16.715621554669315 0.0 27 5.444473179164001E-4 0.0 0.0 17.105718057956416 0.0 28 5.444473179164001E-4 0.0 0.0 17.510242415168303 0.0 29 5.444473179164001E-4 0.0 0.0 17.916672337992896 0.0 30 5.444473179164001E-4 0.0 0.0 18.391158175557038 0.0 31 8.166709768746001E-4 0.0 0.0 18.821271556710993 0.0 32 8.166709768746001E-4 0.0 0.0 19.23042371612517 0.0 33 8.166709768746001E-4 0.0 0.0 19.62215356136602 0.0 34 8.166709768746001E-4 0.0 0.0 20.024772352965197 0.0 35 8.166709768746001E-4 0.0 0.0 20.443724564101867 0.0 36 8.166709768746001E-4 0.0 0.0 20.852332276198123 0.0 37 8.166709768746001E-4 0.0 0.0 21.271012263675836 0.0 38 8.166709768746001E-4 0.0 0.0 21.664103227211477 0.0 39 8.166709768746001E-4 0.0 0.0 22.070805373695027 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGAT 20 7.027011E-4 45.000004 37 GCGGTAT 20 7.027011E-4 45.000004 6 CGTTATC 20 7.027011E-4 45.000004 45 ACGGCTA 20 7.027011E-4 45.000004 34 CGGCTAT 20 7.027011E-4 45.000004 35 ACGTAGG 40 6.7939254E-9 45.000004 2 CGGTATG 20 7.027011E-4 45.000004 7 CGAAAGG 55 1.8189894E-12 45.0 2 ACGGGTC 25 3.885606E-5 45.0 5 AGTAGCG 25 3.885606E-5 45.0 1 TACGTAG 25 3.885606E-5 45.0 1 ACGGTCT 35 1.2089185E-7 45.0 30 CGATTGT 30 2.1614542E-6 44.999996 10 CACGACG 30 2.1614542E-6 44.999996 26 TAGGGCG 145 0.0 43.448273 5 GCGATCA 65 0.0 41.53846 9 AGCGACT 55 6.002665E-11 40.909092 19 TAGGGTC 50 1.0768417E-9 40.5 5 CGTTTTT 1120 0.0 40.379463 1 CGTGCGG 45 1.9221261E-8 40.000004 2 >>END_MODULE