FastQCFastQC Report
Sat 18 Jun 2016
SRR3551565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666982
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189552.841905778566738No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC16790.2517309312695095No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTT14050.2106503623785949No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC11560.1733180205762674No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG11520.17271830424209347No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC10610.15907475763963644No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC10580.158624970389006No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC10570.15847504130546253No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10090.1512784452953753No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG9840.1475302182067882No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT9590.14378199111820109No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC9520.1427324875333967No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9230.13838454411063567No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT7870.11799418874872186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG253.8890375E-545.0000041
TCGTACG253.8890375E-545.00000430
GGTTACG253.8890375E-545.0000041
ACGGGTA207.031154E-445.05
CGGTAAT207.031154E-445.06
CGTTTTT76150.043.4931071
CGTTAGG403.455316E-739.3752
ACAACGA750.039.013
GGCCGAT1400.038.571438
TCTCGTA356.244345E-638.5714328
TATACGG356.244345E-638.571432
ACGGGAC2200.037.8409085
GTTTTTT89950.037.62092
GCCGATC301.139408E-437.5000049
CGATACG301.139408E-437.50000410
ACGGTTA301.139408E-437.50000434
ATTAGTC1100.036.8181831
CAGGTCG250.002106475436.0000041
TAACATA250.002106475436.00000423
CTACGAC250.002106475436.00000428
Produced by FastQC (version 0.11.5)