##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551565_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	666982
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35417297618226	33.0	31.0	34.0	31.0	34.0
2	32.50969141596025	34.0	31.0	34.0	31.0	34.0
3	32.44306443052436	34.0	31.0	34.0	31.0	34.0
4	36.00200155326531	37.0	35.0	37.0	35.0	37.0
5	36.08899040753723	37.0	35.0	37.0	35.0	37.0
6	36.093306865852455	37.0	36.0	37.0	35.0	37.0
7	36.21920831446726	37.0	37.0	37.0	35.0	37.0
8	36.147156894788765	37.0	37.0	37.0	35.0	37.0
9	37.97955716945884	39.0	38.0	39.0	35.0	39.0
10	37.74986431417939	39.0	38.0	39.0	35.0	39.0
11	37.69723620727396	39.0	37.0	39.0	35.0	39.0
12	37.61791772491611	39.0	37.0	39.0	35.0	39.0
13	37.606022351427775	39.0	37.0	39.0	35.0	39.0
14	38.94735090302287	40.0	38.0	41.0	35.0	41.0
15	38.98713008746863	40.0	38.0	41.0	35.0	41.0
16	38.99428620262616	40.0	38.0	41.0	35.0	41.0
17	38.91046984776201	40.0	38.0	41.0	35.0	41.0
18	38.69037545241101	40.0	38.0	41.0	35.0	41.0
19	38.45521918132723	40.0	37.0	41.0	35.0	41.0
20	38.22995972904816	40.0	35.0	41.0	35.0	41.0
21	38.122736445661204	40.0	35.0	41.0	34.0	41.0
22	38.10239106902435	40.0	35.0	41.0	34.0	41.0
23	38.0679643528611	40.0	35.0	41.0	34.0	41.0
24	37.98639543495926	40.0	35.0	41.0	34.0	41.0
25	37.87748245080077	40.0	35.0	41.0	34.0	41.0
26	37.88158151194485	40.0	35.0	41.0	34.0	41.0
27	37.86634271989349	40.0	35.0	41.0	34.0	41.0
28	37.74735300203004	40.0	35.0	41.0	34.0	41.0
29	37.64934286082683	40.0	35.0	41.0	34.0	41.0
30	37.433681868476214	40.0	35.0	41.0	33.0	41.0
31	37.24560183033425	40.0	35.0	41.0	33.0	41.0
32	36.91551796000492	39.0	35.0	41.0	32.0	41.0
33	36.59583017232849	39.0	35.0	41.0	31.0	41.0
34	36.247137104149736	39.0	35.0	41.0	30.0	41.0
35	36.046825851372304	39.0	35.0	41.0	29.0	41.0
36	35.88569406670645	39.0	35.0	41.0	27.0	41.0
37	35.801855222479766	39.0	35.0	41.0	27.0	41.0
38	35.625546116686806	39.0	35.0	41.0	25.0	41.0
39	35.611364324674426	39.0	35.0	41.0	25.0	41.0
40	35.530081771322166	39.0	35.0	41.0	25.0	41.0
41	35.41677886359752	39.0	35.0	41.0	24.0	41.0
42	35.32410319918679	39.0	35.0	41.0	24.0	41.0
43	35.293438203729636	39.0	35.0	41.0	23.0	41.0
44	35.22753387647643	38.0	35.0	41.0	23.0	41.0
45	35.12639021742716	38.0	35.0	41.0	23.0	41.0
46	35.07607401698996	38.0	35.0	41.0	23.0	41.0
47	35.01582051689551	38.0	35.0	41.0	23.0	41.0
48	34.963802621360095	38.0	35.0	40.0	23.0	41.0
49	34.90910849168343	37.0	35.0	40.0	24.0	41.0
50	34.8388577202983	37.0	35.0	40.0	24.0	41.0
51	34.0674695868854	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	14.0
10	12.0
11	14.0
12	13.0
13	14.0
14	32.0
15	40.0
16	62.0
17	108.0
18	195.0
19	326.0
20	585.0
21	917.0
22	1384.0
23	2229.0
24	3621.0
25	6430.0
26	10477.0
27	13748.0
28	13311.0
29	11953.0
30	11419.0
31	11807.0
32	13350.0
33	17586.0
34	34177.0
35	49193.0
36	49845.0
37	64741.0
38	119898.0
39	229210.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.329193291573084	18.252516559667278	22.191753300688774	22.226536848070864
2	39.48127535675626	22.31679415636404	22.17945911583821	16.022471371041497
3	28.81502049530572	22.153521384385186	33.71500280367386	15.31645531663523
4	26.31180451646371	22.316644227280495	34.25999502235443	17.111556233901364
5	24.04982443304317	25.717185771130257	31.71030102761394	18.522688768212635
6	24.43978997933977	30.39362381593506	30.997088377197585	14.16949782752758
7	70.82484984602283	5.706300919664999	18.754329202287316	4.714520032024852
8	71.59218689559839	5.590705596252973	17.933317540803202	4.883789967345446
9	65.22694765375977	7.194796861084707	20.128429252963347	7.449826232192174
10	35.509953791856454	23.570201294787566	25.779256411717256	15.140588501638724
11	27.52098257524191	21.523969162586095	32.01900501062997	18.93604325154202
12	24.48866686057495	18.875921689041082	36.21836871159941	20.417042738784556
13	23.02910723227913	19.214761417849356	38.451562410979605	19.304568938891904
14	20.353772665529206	22.15711968239023	36.94897313570681	20.540134516373758
15	19.66454866847981	21.208518370810605	38.89235391659745	20.234579044112134
16	22.602858847765006	21.201021916633433	36.28628658644461	19.90983264915695
17	22.245277983513798	21.72592363811917	34.792393198017336	21.236405180349692
18	22.952193612421325	21.395479937989332	34.29133020081501	21.36099624877433
19	22.802264528877842	23.275440716541077	33.28665541199013	20.635639342590956
20	23.521474342635933	23.108269788390093	33.517846058814186	19.852409810159795
21	23.3048268169156	23.80094215436099	33.690114575805644	19.20411645291777
22	21.63176817365386	21.648710160094275	34.20422140327625	22.515300262975614
23	21.960112866614093	22.721602681931447	34.04934465997583	21.26893979147863
24	22.466723239907523	21.857411444386805	34.15639402562588	21.519471290079792
25	22.793868500199405	22.813059422892973	32.64750772884425	21.745564348063365
26	21.286331565169675	22.603608493182726	33.48366222776627	22.626397713881335
27	22.92880467538854	21.494583062211575	33.31694108686592	22.259671175533974
28	22.186055995514124	22.501356858206066	33.66552620610451	21.647060940175297
29	23.90049506583386	21.860410026057675	32.02785082655904	22.211244081549427
30	24.43978997933977	21.489335544287552	32.380633960136855	21.69024051623582
31	24.481320335481318	22.60690693302068	31.018978023394933	21.892794708103068
32	23.914888257854034	23.127760569250743	31.093492777916044	21.863858394979175
33	23.306026249583947	22.769879846832445	30.031994866428178	23.892099037155425
34	22.31259614202482	23.700189810219765	31.13157476513609	22.85563928261932
35	22.7445418317136	24.38251706942616	29.746379962277842	23.126561136582396
36	21.962061944700157	26.434896294052912	29.50619357044118	22.096848190805748
37	22.281560821731322	26.314953027218124	30.03349415726361	21.36999199378694
38	21.18407993019302	26.359332035947	29.64772662530623	22.808861408553753
39	22.223688195483536	24.430494376160077	29.752377125619585	23.593440302736806
40	22.124735000344835	24.49556359841795	31.26980938016318	22.10989202107403
41	20.500853096485365	25.752269176679434	31.00113646245326	22.745741264381948
42	21.102218650578276	25.71793541654797	30.642656023700788	22.537189909172962
43	22.38381245670798	22.963438293687087	31.580912228515913	23.071837021089024
44	22.888023964664715	23.038102977291743	30.652551343214657	23.421321714828885
45	22.649636721830575	22.501506787289614	30.33545133152019	24.51340515935962
46	22.630595728220552	23.813086410128008	30.141143239247835	23.415174622403605
47	21.737318248468473	23.658959312245308	32.53850928510814	22.065213154178075
48	21.21601482498778	22.967336449859214	32.52201708591836	23.29463163923464
49	21.23235709509402	22.541537852595724	32.85530943863553	23.370795613674733
50	20.504901181741037	21.98680024348483	33.710355002084015	23.797943572690116
51	21.026354534305273	22.260720679118776	31.86097975657514	24.85194503000081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	325.0
1	817.5
2	1310.0
3	14860.0
4	28410.0
5	17736.0
6	7062.0
7	6831.5
8	6601.0
9	6410.5
10	6220.0
11	6014.5
12	5809.0
13	5545.0
14	5281.0
15	4934.5
16	4588.0
17	4214.5
18	3841.0
19	3602.0
20	3363.0
21	3285.5
22	3208.0
23	3173.5
24	3139.0
25	3552.5
26	4475.5
27	4985.0
28	5581.0
29	6177.0
30	6971.5
31	7766.0
32	8520.0
33	9274.0
34	10283.5
35	11293.0
36	12143.0
37	12993.0
38	13899.0
39	14805.0
40	17066.0
41	19327.0
42	21727.5
43	24128.0
44	27312.5
45	30497.0
46	40432.5
47	50368.0
48	54003.0
49	57638.0
50	57019.5
51	56401.0
52	51441.5
53	46482.0
54	43193.0
55	39904.0
56	37587.0
57	35270.0
58	33944.0
59	32618.0
60	31443.0
61	30268.0
62	28455.0
63	26642.0
64	24084.5
65	21527.0
66	18261.5
67	14996.0
68	12764.0
69	10532.0
70	8838.5
71	7145.0
72	6115.0
73	5085.0
74	4296.0
75	2819.5
76	2132.0
77	1597.0
78	1062.0
79	867.0
80	672.0
81	449.0
82	226.0
83	157.5
84	89.0
85	63.0
86	37.0
87	22.0
88	7.0
89	4.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.47265645702093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75843691302562	26.172208174215932
2	7.899519796023831	5.762329433916265
3	3.717869874774292	4.068017720836505
4	2.563837201426087	3.740398138373745
5	2.118998504042487	3.8642752235441455
6	1.754947779946497	3.840456448680009
7	1.5685804284065816	4.004720656333598
8	1.32223385212079	3.8580304835395984
9	1.1644050492954972	3.8222050805797667
>10	6.038130783129151	31.91132198781219
>50	0.052929318697784195	1.3347409974543458
>100	0.03266731370930113	2.309026506909734
>500	0.003721592701059621	1.0091245171608771
>1k	0.003308082400941885	1.4443858168957884
>5k	0.0	0.0
>10k+	4.1351030011773564E-4	2.8587588137474853
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18955	2.841905778566738	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	1679	0.2517309312695095	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTT	1405	0.2106503623785949	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC	1156	0.1733180205762674	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	1152	0.17271830424209347	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	1061	0.15907475763963644	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1058	0.158624970389006	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	1057	0.15847504130546253	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1009	0.1512784452953753	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	984	0.1475302182067882	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	959	0.14378199111820109	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	952	0.1427324875333967	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	923	0.13838454411063567	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	787	0.11799418874872186	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4992908354348393E-4	0.0	0.0	0.12354156483983077	0.0
2	1.4992908354348393E-4	0.0	0.0	0.532548104746455	0.0
3	1.4992908354348393E-4	0.0	0.0	0.9108191825266649	0.0
4	1.4992908354348393E-4	0.0	0.0	1.3382669997091377	0.0
5	1.4992908354348393E-4	0.0	0.0	2.5591395270037274	0.0
6	1.4992908354348393E-4	0.0	0.0	3.8273896446980578	0.0
7	1.4992908354348393E-4	0.0	0.0	4.8166217379179646	0.0
8	1.4992908354348393E-4	0.0	0.0	6.096866182295774	0.0
9	1.4992908354348393E-4	0.0	0.0	6.886542665319304	0.0
10	1.4992908354348393E-4	0.0	0.0	8.094521291429155	0.0
11	1.4992908354348393E-4	0.0	0.0	9.193201615635804	0.0
12	1.4992908354348393E-4	0.0	0.0	10.164442218830493	0.0
13	1.4992908354348393E-4	0.0	0.0	10.585143227253509	0.0
14	1.4992908354348393E-4	0.0	0.0	10.797892596801713	0.0
15	1.4992908354348393E-4	0.0	0.0	11.067315159929354	0.0
16	1.4992908354348393E-4	0.0	0.0	11.46567673490439	0.0
17	1.4992908354348393E-4	0.0	0.0	11.892674764836233	0.0
18	1.4992908354348393E-4	0.0	0.0	12.405282301471404	0.0
19	0.0016492199189783233	0.0	0.0	12.749369548203699	0.0
20	0.0016492199189783233	0.0	0.0	13.06482033997919	0.0
21	0.0016492199189783233	0.0	0.0	13.396013685526746	0.0
22	0.0016492199189783233	0.0	0.0	13.77488447964113	0.0
23	0.0016492199189783233	0.0	0.0	14.14326023790747	0.0
24	0.0016492199189783233	0.0	0.0	14.448965639252634	0.0
25	0.0016492199189783233	0.0	0.0	14.74012791949408	0.0
26	0.0016492199189783233	0.0	0.0	15.019295873052046	0.0
27	0.0016492199189783233	0.0	0.0	15.311057869627666	0.0
28	0.0016492199189783233	0.0	0.0	15.60296979528683	0.0
29	0.0016492199189783233	0.0	0.0	15.908375338464905	0.0
30	0.001799149002521807	0.0	0.0	16.26235790471107	0.0
31	0.001799149002521807	0.0	0.0	16.55052160328165	0.0
32	0.001799149002521807	0.0	0.0	16.872119487482422	0.0
33	0.001799149002521807	0.0	0.0	17.195066733435084	0.0
34	0.001799149002521807	0.0	0.0	17.481581212086684	0.0
35	0.001799149002521807	0.0	0.0	17.796132429360913	0.0
36	0.001799149002521807	0.0	0.0	18.10033853987064	0.0
37	0.001799149002521807	0.0	0.0	18.401695997793045	0.0
38	0.001799149002521807	0.0	0.0	18.737387215846905	0.0
39	0.001799149002521807	0.0	0.0	19.04923970961735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	25	3.8890375E-5	45.000004	1
TCGTACG	25	3.8890375E-5	45.000004	30
GGTTACG	25	3.8890375E-5	45.000004	1
ACGGGTA	20	7.031154E-4	45.0	5
CGGTAAT	20	7.031154E-4	45.0	6
CGTTTTT	7615	0.0	43.493107	1
CGTTAGG	40	3.455316E-7	39.375	2
ACAACGA	75	0.0	39.0	13
GGCCGAT	140	0.0	38.57143	8
TCTCGTA	35	6.244345E-6	38.57143	28
TATACGG	35	6.244345E-6	38.57143	2
ACGGGAC	220	0.0	37.840908	5
GTTTTTT	8995	0.0	37.6209	2
GCCGATC	30	1.139408E-4	37.500004	9
CGATACG	30	1.139408E-4	37.500004	10
ACGGTTA	30	1.139408E-4	37.500004	34
ATTAGTC	110	0.0	36.81818	31
CAGGTCG	25	0.0021064754	36.000004	1
TAACATA	25	0.0021064754	36.000004	23
CTACGAC	25	0.0021064754	36.000004	28
>>END_MODULE