FastQCFastQC Report
Sat 18 Jun 2016
SRR3551564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555318
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.6354917362664275No Hit
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC21600.3889663219992869RNA PCR Primer, Index 14 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC15050.27101588639302165No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG14310.2576901883245276No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC13820.2488664152791734No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT12950.23319971619864657Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC12330.22203494214125963Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC9130.16441030184506897No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8260.1487436027645421No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC7350.1323565956803129RNA PCR Primer, Index 14 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA7330.1319964416784617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG650.045.0000042
ATTCGCT253.8881917E-545.00000413
ATATGCG253.8881917E-545.0000041
TACGCGG351.2104101E-745.02
GTAGACG207.030133E-445.01
TTAACGG207.030133E-445.02
CGGTCTA351.2104101E-745.031
CAGTACG207.030133E-445.01
GGTTCGA207.030133E-445.038
CCACGAA207.030133E-445.018
CGATATA207.030133E-445.012
AGTCGTG207.030133E-445.01
ATCGCAT302.1634678E-644.99999610
CGAATAT950.042.6315814
CGAAAGG556.002665E-1140.9090922
CGTTTTT16050.039.953271
CTCACGA403.4539153E-739.37524
CGTTCAT403.4539153E-739.37517
CTGACTA1100.038.86363622
CCAAACT1450.038.79310218