##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	555318
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.250272816656405	33.0	31.0	34.0	30.0	34.0
2	32.37059126482484	34.0	31.0	34.0	30.0	34.0
3	32.377900590292406	34.0	31.0	34.0	30.0	34.0
4	35.91961542755682	37.0	35.0	37.0	35.0	37.0
5	36.00761905790916	37.0	35.0	37.0	35.0	37.0
6	36.021699278611536	37.0	35.0	37.0	35.0	37.0
7	36.155109324747265	37.0	36.0	37.0	35.0	37.0
8	36.03422543479592	37.0	36.0	37.0	35.0	37.0
9	37.8720624939224	39.0	38.0	39.0	35.0	39.0
10	37.60516136700053	39.0	37.0	39.0	35.0	39.0
11	37.57689468016524	39.0	37.0	39.0	35.0	39.0
12	37.52063862507608	39.0	37.0	39.0	35.0	39.0
13	37.470096413226294	39.0	37.0	39.0	35.0	39.0
14	38.761988626336624	40.0	38.0	41.0	35.0	41.0
15	38.81786471895383	40.0	38.0	41.0	35.0	41.0
16	38.84321595914413	40.0	38.0	41.0	35.0	41.0
17	38.77686478738308	40.0	38.0	41.0	35.0	41.0
18	38.717315123947	40.0	38.0	41.0	35.0	41.0
19	38.65901339412733	40.0	38.0	41.0	35.0	41.0
20	38.55041795871915	40.0	38.0	41.0	35.0	41.0
21	38.44310287078755	40.0	37.0	41.0	34.0	41.0
22	38.4382876117828	40.0	37.0	41.0	34.0	41.0
23	38.38757792832215	40.0	37.0	41.0	34.0	41.0
24	38.287049942555434	40.0	37.0	41.0	34.0	41.0
25	38.16989724806327	40.0	37.0	41.0	34.0	41.0
26	38.19087441790109	40.0	37.0	41.0	34.0	41.0
27	38.176927454179406	40.0	37.0	41.0	34.0	41.0
28	38.09784483845292	40.0	37.0	41.0	34.0	41.0
29	38.05212688945793	40.0	37.0	41.0	34.0	41.0
30	37.901865237575585	40.0	36.0	41.0	34.0	41.0
31	37.87182299151117	40.0	36.0	41.0	34.0	41.0
32	37.76685610767164	40.0	36.0	41.0	33.0	41.0
33	37.67072200072751	40.0	36.0	41.0	33.0	41.0
34	37.51272063934538	40.0	36.0	41.0	33.0	41.0
35	37.44960905283099	40.0	36.0	41.0	33.0	41.0
36	37.36251481133333	40.0	36.0	41.0	33.0	41.0
37	37.28652771925275	40.0	35.0	41.0	33.0	41.0
38	37.11377625072481	40.0	35.0	41.0	32.0	41.0
39	37.11661786579941	40.0	35.0	41.0	32.0	41.0
40	37.034065166265094	39.0	35.0	41.0	32.0	41.0
41	36.92463957588265	39.0	35.0	41.0	32.0	41.0
42	36.8697665121606	39.0	35.0	41.0	32.0	41.0
43	36.847890758088155	39.0	35.0	41.0	32.0	41.0
44	36.798598280624795	39.0	35.0	41.0	32.0	41.0
45	36.68750517721378	39.0	35.0	41.0	31.0	41.0
46	36.615920967805835	39.0	35.0	41.0	31.0	41.0
47	36.550383744088975	39.0	35.0	41.0	31.0	41.0
48	36.527776877392775	39.0	35.0	41.0	31.0	41.0
49	36.48242628547967	39.0	35.0	41.0	31.0	41.0
50	36.39953504118361	39.0	35.0	41.0	31.0	41.0
51	35.59335551881985	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	10.0
10	17.0
11	5.0
12	9.0
13	9.0
14	15.0
15	29.0
16	44.0
17	83.0
18	131.0
19	195.0
20	365.0
21	628.0
22	1008.0
23	1413.0
24	2070.0
25	3001.0
26	4095.0
27	5082.0
28	5541.0
29	5782.0
30	6588.0
31	8132.0
32	10448.0
33	14426.0
34	28099.0
35	40441.0
36	39285.0
37	58617.0
38	108246.0
39	211250.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33358183959461	20.406145667887586	23.460971911589397	13.799300580928406
2	32.52424736817463	25.2381518337241	24.542694456149448	17.694906341951818
3	31.462153216715468	24.435728717599645	27.247991241054674	16.85412682463021
4	28.536802336679163	24.260693872699967	27.683957660295544	19.518546130325326
5	25.820881008719326	27.99891233491441	24.995047882474548	21.185158773891715
6	25.78594607053976	34.10532343630136	24.733756154131505	15.374974339027366
7	76.46591682603481	6.6021630849351185	11.640537493832364	5.291382595197707
8	75.59434414155493	7.294559153494035	11.140283585261058	5.970813119689979
9	68.8781923150339	8.66116351351838	13.857105298225521	8.60353887322219
10	38.27248531472057	25.06977983785867	20.62277830000108	16.034956547419675
11	29.08729772850871	24.187042379321397	25.79134838056753	20.93431151160236
12	26.23451787984542	20.801594761920196	31.42145581450628	21.5424315437281
13	23.690029856766753	22.927043603845004	32.22171800661963	21.16120853276861
14	20.434597834033834	26.90260355327938	29.89476300065908	22.768035612027703
15	19.792083094731307	24.25313063866109	34.30340813731952	21.651378129288084
16	21.452933274268077	23.78421012825084	31.653394991698452	23.109461605782634
17	21.05370256321603	24.54791668917629	29.05290302133192	25.345477726275757
18	21.75204117280549	23.67382292668345	30.817657630402763	23.756478270108296
19	23.581623502209546	24.781296482375865	28.22652966408436	23.41055035133023
20	24.7393385411602	24.91149215404507	28.445323220208962	21.90384608458577
21	23.6432098365261	26.115847136235455	28.293878462430534	21.94706456480791
22	22.23212645727313	24.03235623552631	27.150389506553	26.585127800647555
23	22.105892479624288	25.547524121314275	28.117763155525306	24.228820243536138
24	22.48117294955323	24.25493140867035	29.246485797326937	24.017409844449485
25	21.809665813101685	26.292142520141613	27.187305291742746	24.710886375013956
26	21.015526239019806	25.77622191248978	27.408259771878456	25.79999207661196
27	23.059940430528094	25.2606614588398	27.79326439985738	23.886133710774725
28	20.908740577470926	25.492240482030116	28.52977933364307	25.06923960685589
29	21.422320184110728	23.97761282724493	26.68326976615201	27.91679722249234
30	22.235187766288867	25.391397361511782	26.2343378028445	26.139077069354855
31	22.32324541974148	27.25303339708059	23.8099611393832	26.613760043794727
32	21.685952913465798	26.239019804868562	24.857829207769242	27.217198073896398
33	21.560079089818807	25.220324210632466	24.56880562128366	28.650791078265065
34	20.195995807807417	26.571261871576286	25.79909169160733	27.433650629008966
35	21.484086595428206	23.894417252817306	26.18427639658718	28.43721975516731
36	21.152924990726035	26.46843790404777	26.10054059115678	26.278096514069414
37	20.377153270738567	27.569608764707787	27.367742446670196	24.685495517883446
38	19.833500804944194	27.69206112533719	26.906205093297896	25.568232976420717
39	21.414036642068147	24.555119769213317	28.239675285151932	25.791168303566604
40	21.432044342160708	24.13788135806871	29.66948667250116	24.76058762726942
41	19.253292707961926	25.06761891384756	29.068569720412444	26.610518657778066
42	19.859611970078404	24.265375874724032	30.022437594315328	25.852574560882235
43	21.964171879895844	23.066603279562344	28.853197627305438	26.116027213236382
44	21.224415560093497	24.15102697913628	27.15777266359095	27.466784797179272
45	21.42051941410147	23.10477960375857	26.829312213902668	28.6453887682373
46	22.169819814952874	24.508839979975438	27.655865648151153	25.665474556920536
47	20.03806827799567	24.19766692237601	30.442197083472895	25.322067716155427
48	19.85006788902935	23.704436016840802	29.170493302936336	27.275002791193515
49	20.754594664678617	23.413251506344114	30.397718064244273	25.434435764733
50	20.66941824324081	22.623613857285374	29.714685999733486	26.992281899740327
51	20.06796106014932	23.100817909738204	27.592658620826267	29.23856240928621
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	657.0
2	1016.0
3	3312.0
4	5608.0
5	3723.0
6	1838.0
7	1844.0
8	1850.0
9	1784.0
10	1718.0
11	1754.5
12	1791.0
13	1781.5
14	1772.0
15	1721.0
16	1670.0
17	1627.5
18	1585.0
19	1618.0
20	1651.0
21	1659.5
22	1668.0
23	1871.0
24	2074.0
25	2633.5
26	3967.0
27	4741.0
28	5239.0
29	5737.0
30	6747.5
31	7758.0
32	8876.5
33	9995.0
34	10578.0
35	11161.0
36	12220.5
37	13280.0
38	13794.0
39	14308.0
40	16864.0
41	19420.0
42	21866.0
43	24312.0
44	27588.5
45	30865.0
46	40228.0
47	49591.0
48	53107.5
49	56624.0
50	54840.5
51	53057.0
52	46351.5
53	39646.0
54	35815.5
55	31985.0
56	29471.5
57	26958.0
58	26429.0
59	25900.0
60	24797.0
61	23694.0
62	22246.5
63	20799.0
64	18948.0
65	17097.0
66	14718.0
67	12339.0
68	10455.5
69	8572.0
70	7874.5
71	7177.0
72	6167.5
73	5158.0
74	4154.5
75	2607.5
76	2064.0
77	1626.5
78	1189.0
79	857.5
80	526.0
81	381.5
82	237.0
83	188.0
84	139.0
85	88.5
86	38.0
87	34.5
88	31.0
89	31.0
90	31.0
91	16.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	555318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.65524080516923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69133792276791	23.010618063951487
2	9.461777278193354	5.990296763721786
3	3.3917329413001176	3.2209836901104043
4	1.8047651307245898	2.285210992394384
5	1.1828134611245267	1.8721122469746283
6	0.9706760414821337	1.8436190302155226
7	0.795966590643379	1.7637559869780002
8	0.7426311435382073	1.8806534142496112
9	0.6867490758664121	1.9565286632350833
>10	8.117692798753522	47.96769627888061
>50	0.09494559178652605	2.00772729284117
>100	0.05147652566739353	3.159785076622242
>500	0.0034317683778262355	0.7714785819096593
>1k	0.004003729774130608	2.269533917915414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3529	0.6354917362664275	No Hit
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	2160	0.3889663219992869	RNA PCR Primer, Index 14 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	1505	0.27101588639302165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	1431	0.2576901883245276	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	1382	0.2488664152791734	No Hit
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	1295	0.23319971619864657	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	1233	0.22203494214125963	Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	913	0.16441030184506897	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	826	0.1487436027645421	No Hit
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	735	0.1323565956803129	RNA PCR Primer, Index 14 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	733	0.1319964416784617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.00385004627979E-4	0.0	0.0	0.24130318124029834	0.0
2	9.00385004627979E-4	0.0	0.0	1.1024313996664974	0.0
3	9.00385004627979E-4	0.0	0.0	1.5248920438379452	0.0
4	9.00385004627979E-4	0.0	0.0	2.1128434518600154	0.0
5	9.00385004627979E-4	0.0	0.0	3.828076885676315	0.0
6	0.0010804620055535747	0.0	0.0	4.885669112112339	0.0
7	0.0010804620055535747	0.0	0.0	5.896621395308634	0.0
8	0.0010804620055535747	0.0	0.0	7.511191785607526	0.0
9	0.0010804620055535747	0.0	0.0	8.20160700715626	0.0
10	0.0010804620055535747	0.0	0.0	9.605847460374056	0.0
11	0.0010804620055535747	0.0	0.0	11.122455962169424	0.0
12	0.0010804620055535747	0.0	0.0	12.536960804439978	0.0
13	0.0010804620055535747	0.0	0.0	13.031452248981665	0.0
14	0.0010804620055535747	0.0	0.0	13.23115764300815	0.0
15	0.0010804620055535747	0.0	0.0	13.648756208154607	0.0
16	0.0010804620055535747	0.0	0.0	14.349075664754249	0.0
17	0.0010804620055535747	0.0	0.0	15.187514181063824	0.0
18	0.0010804620055535747	0.0	0.0	16.108067809795468	0.0
19	0.0010804620055535747	0.0	0.0	16.67999236473516	0.0
20	0.0010804620055535747	0.0	0.0	17.21103944046474	0.0
21	0.0010804620055535747	0.0	0.0	17.81177631555253	0.0
22	0.0010804620055535747	0.0	0.0	18.445287204808775	0.0
23	0.0010804620055535747	0.0	0.0	19.070874706024295	0.0
24	0.0010804620055535747	0.0	0.0	19.596339394725184	0.0
25	0.0010804620055535747	0.0	0.0	20.06381928912803	0.0
26	0.0010804620055535747	0.0	0.0	20.49492362934391	0.0
27	0.0010804620055535747	0.0	0.0	20.947997363672705	0.0
28	0.0010804620055535747	0.0	0.0	21.36775685283027	0.0
29	0.0010804620055535747	0.0	0.0	21.806424427085023	0.0
30	0.0010804620055535747	0.0	0.0	22.313881415693352	0.0
31	0.0010804620055535747	0.0	0.0	22.761732916995307	0.0
32	0.0010804620055535747	0.0	0.0	23.21390626631948	0.0
33	0.0010804620055535747	0.0	0.0	23.65005276256127	0.0
34	0.0010804620055535747	0.0	0.0	24.06386971068829	0.0
35	0.0010804620055535747	0.0	0.0	24.52144537004023	0.0
36	0.0010804620055535747	0.0	0.0	24.946787246226485	0.0
37	0.0012605390064791705	0.0	0.0	25.368527582394233	0.0
38	0.0012605390064791705	0.0	0.0	25.80647484864528	0.0
39	0.0014406160074047663	0.0	0.0	26.219931642770447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	65	0.0	45.000004	2
ATTCGCT	25	3.8881917E-5	45.000004	13
ATATGCG	25	3.8881917E-5	45.000004	1
TACGCGG	35	1.2104101E-7	45.0	2
GTAGACG	20	7.030133E-4	45.0	1
TTAACGG	20	7.030133E-4	45.0	2
CGGTCTA	35	1.2104101E-7	45.0	31
CAGTACG	20	7.030133E-4	45.0	1
GGTTCGA	20	7.030133E-4	45.0	38
CCACGAA	20	7.030133E-4	45.0	18
CGATATA	20	7.030133E-4	45.0	12
AGTCGTG	20	7.030133E-4	45.0	1
ATCGCAT	30	2.1634678E-6	44.999996	10
CGAATAT	95	0.0	42.63158	14
CGAAAGG	55	6.002665E-11	40.909092	2
CGTTTTT	1605	0.0	39.95327	1
CTCACGA	40	3.4539153E-7	39.375	24
CGTTCAT	40	3.4539153E-7	39.375	17
CTGACTA	110	0.0	38.863636	22
CCAAACT	145	0.0	38.793102	18
>>END_MODULE