FastQCFastQC Report
Sat 18 Jun 2016
SRR3551563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415802
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105232.530771857759222No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC7340.17652632743469246No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG5930.14261595663320523No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC5840.1404514648799188No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT5680.13660347954074295No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC5560.13371749053636106No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT5470.13155299878307464Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5390.1296290061134867No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC4950.11904704643075309No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4460.10726259132952704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAG302.1621472E-644.9999961
CGTTTTT40650.042.8966831
TACGAAT700.041.78571712
ATACGAA556.002665E-1140.90909219
CATATTA501.0786607E-940.528
GGTATGC850.039.7058838
TAGGGAT2050.039.5121965
TATACGG403.4511322E-739.3750042
CTGACTA750.039.022
TACTGAC750.039.020
CGAATAT750.039.014
GCTACGA750.039.010
GTAGACG356.2386734E-638.571431
AAGGGAT2800.037.767865
CATACGA601.546141E-1037.49999618
AAGCATA552.741217E-936.8181825
TATGGGC800.036.5625044
AGTGCAG800.036.5625041
AAGCTAC800.036.5625048
GTTTTTT50100.036.2425162
Produced by FastQC (version 0.11.5)