##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415802
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.413321725244224	33.0	31.0	34.0	31.0	34.0
2	32.57013434278815	34.0	31.0	34.0	31.0	34.0
3	32.51894651781377	34.0	31.0	34.0	31.0	34.0
4	36.046738591925966	37.0	35.0	37.0	35.0	37.0
5	36.125699251085855	37.0	35.0	37.0	35.0	37.0
6	36.123989302600755	37.0	36.0	37.0	35.0	37.0
7	36.237543349959836	37.0	37.0	37.0	35.0	37.0
8	36.176737966628345	37.0	37.0	37.0	35.0	37.0
9	38.00743623166796	39.0	39.0	39.0	35.0	39.0
10	37.775412335679	39.0	38.0	39.0	35.0	39.0
11	37.70943862703883	39.0	37.0	39.0	35.0	39.0
12	37.651158484086174	39.0	37.0	39.0	35.0	39.0
13	37.649489420445306	39.0	37.0	39.0	35.0	39.0
14	39.0228762728414	40.0	39.0	41.0	35.0	41.0
15	39.0790785037109	40.0	39.0	41.0	35.0	41.0
16	39.073939038292266	41.0	39.0	41.0	35.0	41.0
17	39.0232466414303	40.0	38.0	41.0	35.0	41.0
18	38.8253784253082	40.0	38.0	41.0	35.0	41.0
19	38.618118719967676	40.0	37.0	41.0	35.0	41.0
20	38.41669592738852	40.0	36.0	41.0	35.0	41.0
21	38.30631646793426	40.0	36.0	41.0	35.0	41.0
22	38.28688654696226	40.0	36.0	41.0	35.0	41.0
23	38.23443369680761	40.0	36.0	41.0	35.0	41.0
24	38.12806576206945	40.0	35.0	41.0	35.0	41.0
25	38.01034867557155	40.0	35.0	41.0	34.0	41.0
26	38.00726547731853	40.0	35.0	41.0	34.0	41.0
27	37.991924040769405	40.0	35.0	41.0	34.0	41.0
28	37.896633493826386	40.0	35.0	41.0	34.0	41.0
29	37.78715350094516	40.0	35.0	41.0	34.0	41.0
30	37.58917465524456	40.0	35.0	41.0	33.0	41.0
31	37.44664287329065	40.0	35.0	41.0	33.0	41.0
32	37.16470339248008	40.0	35.0	41.0	33.0	41.0
33	36.86743450007455	40.0	35.0	41.0	32.0	41.0
34	36.57220263490796	40.0	35.0	41.0	31.0	41.0
35	36.37586399295819	40.0	35.0	41.0	30.0	41.0
36	36.22159826071063	40.0	35.0	41.0	29.0	41.0
37	36.12053333076801	39.0	35.0	41.0	29.0	41.0
38	35.97130845931477	39.0	35.0	41.0	28.0	41.0
39	35.907172163674055	39.0	35.0	41.0	28.0	41.0
40	35.80910385231432	39.0	35.0	41.0	27.0	41.0
41	35.69992207829688	39.0	35.0	41.0	26.0	41.0
42	35.563768332042656	39.0	35.0	41.0	26.0	41.0
43	35.5116137007518	39.0	35.0	41.0	25.0	41.0
44	35.45771545110413	39.0	35.0	41.0	25.0	41.0
45	35.372453715951345	38.0	35.0	41.0	24.0	41.0
46	35.31921443379301	38.0	35.0	41.0	24.0	41.0
47	35.286667692796094	38.0	35.0	41.0	25.0	41.0
48	35.21585273760107	38.0	35.0	41.0	25.0	41.0
49	35.14009312124521	38.0	35.0	41.0	25.0	41.0
50	35.03882857706312	37.0	35.0	40.0	24.0	41.0
51	34.255919884945236	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	9.0
11	9.0
12	14.0
13	11.0
14	18.0
15	34.0
16	42.0
17	88.0
18	123.0
19	216.0
20	344.0
21	555.0
22	811.0
23	1266.0
24	2022.0
25	3666.0
26	6059.0
27	7730.0
28	7920.0
29	6918.0
30	6528.0
31	6719.0
32	7833.0
33	10527.0
34	19965.0
35	29265.0
36	30639.0
37	41095.0
38	73524.0
39	151646.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.77086209301543	18.909480954877562	21.959490334341826	21.360166617765188
2	38.30452955974238	22.814945575057358	22.56290253534134	16.317622329858924
3	29.66532147512518	22.223077330075373	32.145588525307716	15.96601266949173
4	26.761535538549598	23.698539208565617	32.102298690241994	17.437626562642798
5	23.64346491839866	27.606408819582395	30.4909548294621	18.25917143255684
6	25.211278445029123	31.35242254727009	29.3269392643614	14.109359743339379
7	72.85029894036103	5.950428328868067	16.52252754917004	4.676745181600858
8	73.99170759159408	5.4100268877975575	16.13508352533177	4.463181995276598
9	67.58120451561079	7.671680270898168	18.311359733719414	6.435755479771623
10	36.753310469887104	24.473186757158455	23.97992313649285	14.793579636461585
11	27.541955065151203	22.030918562200277	31.2396284770155	19.18749789563302
12	24.231966176208868	19.69110297689766	35.60107935988764	20.475851487005833
13	22.700468011216877	20.38061384986123	36.70545115223111	20.213466986690783
14	20.006878273793777	23.060014141346123	35.61142082048667	21.321686764373425
15	19.9231845926667	22.257468699044257	36.74200701295328	21.077339695335763
16	21.380128041712158	21.73606668558593	35.565725994583964	21.31807927811795
17	21.79306496842247	21.90778303134665	33.74033794931242	22.558814050918468
18	21.923415471787052	22.231013799837424	34.01715239464937	21.828418333726148
19	22.855108922035008	23.070115102861458	32.65977556625509	21.415000408848442
20	23.53067084814407	22.713214462652896	32.782189599857624	20.97392508934541
21	23.569150701535825	23.291133760780372	32.47170528280287	20.66801025488093
22	22.62519179801925	21.98570473446496	32.19513133654961	23.19397213096618
23	22.08334736244655	22.906816225030184	32.539526024405845	22.47031038811742
24	21.82649434105656	22.819274558563933	33.3437549602936	22.010476140085906
25	22.162231061899654	23.81085228065281	31.915190403124566	22.11172625432297
26	21.13794546442778	24.423884445000265	31.907494432446214	22.530675658125745
27	22.788731174934224	22.949144063761118	32.641497635894005	21.620627125410653
28	21.07709919625206	24.205030278834638	32.68069898653686	22.037171538376437
29	21.609083169393127	23.713450151754923	31.782434908922998	22.895031769928956
30	22.008792646500016	24.501806148118575	31.726398622421247	21.76300258296016
31	22.944815080254543	24.322153332595803	30.465702425673758	22.267329161475896
32	22.44385548891059	25.07852295082756	30.312745008441517	22.16487655182034
33	22.28079711016301	24.86014978282933	29.692978869750508	23.166074237257156
34	21.45516375582609	25.029942135920464	30.388021221639143	23.1268728866143
35	23.20407309248152	24.344760246463462	29.119629054213302	23.331537606841717
36	23.35630901246266	26.470531647274427	28.386106848932908	21.78705249133001
37	23.425813247651526	26.00300142856456	28.867586014497288	21.70359930928663
38	23.039090721064355	25.491940875705264	29.42434139325929	22.044627009971094
39	23.639376433975787	24.16967691353096	29.188171293067374	23.002775359425883
40	23.120138912270743	24.36568366674523	30.418564605268855	22.09561281571517
41	22.235583282427694	24.65404206809972	29.715345284534468	23.395029364938118
42	22.00783065016522	25.900548818908998	29.306256343163334	22.785364187762443
43	22.874589347814585	23.892621969110298	30.440450021885418	22.7923386611897
44	22.412109609862384	24.347165237300448	29.740597688322808	23.50012746451436
45	22.462133419271673	23.57684667221418	29.786052015141824	24.174967893372326
46	22.132409175521044	24.457313817634354	29.907985050576958	23.502291956267644
47	21.74688914435236	24.65572556168561	31.025584292523845	22.571801001438185
48	21.840202788827376	23.977037147488467	30.851703455009837	23.331056608674324
49	21.747129643436057	24.20286578708135	31.393788389666234	22.656216179816354
50	20.97873507101938	23.582859149306643	32.29397645994968	23.144429319724292
51	21.643955536529404	23.357511507881153	30.508270763488394	24.49026219210105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	602.5
2	975.0
3	8349.0
4	15723.0
5	9908.0
6	4093.0
7	3960.0
8	3827.0
9	3738.0
10	3649.0
11	3503.5
12	3358.0
13	3261.0
14	3164.0
15	2938.5
16	2713.0
17	2514.5
18	2316.0
19	2210.5
20	2105.0
21	2045.0
22	1985.0
23	1961.5
24	1938.0
25	2302.0
26	2960.0
27	3254.0
28	3978.5
29	4703.0
30	5324.5
31	5946.0
32	6415.0
33	6884.0
34	7646.5
35	8409.0
36	9032.5
37	9656.0
38	10243.5
39	10831.0
40	12254.5
41	13678.0
42	14991.0
43	16304.0
44	18415.5
45	20527.0
46	24065.5
47	27604.0
48	30437.5
49	33271.0
50	32430.0
51	31589.0
52	28411.0
53	25233.0
54	23588.5
55	21944.0
56	21750.5
57	21557.0
58	21182.0
59	20807.0
60	19751.0
61	18695.0
62	18075.0
63	17455.0
64	15858.5
65	14262.0
66	12305.0
67	10348.0
68	9131.0
69	7914.0
70	6842.5
71	5771.0
72	4971.0
73	4171.0
74	3533.5
75	2252.5
76	1609.0
77	1223.5
78	838.0
79	644.5
80	451.0
81	376.0
82	301.0
83	194.5
84	88.0
85	73.5
86	59.0
87	31.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.92648703463526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75044824176067	26.453894709011333
2	8.083516021575592	6.131587311731062
3	4.199400611346277	4.77805538518392
4	3.207396126037412	4.8658107035638904
5	2.6124682625216504	4.954087184346174
6	2.1627540561474428	4.921539820174847
7	1.8272201226871905	4.851002820476427
8	1.645368994507165	4.992245266989348
9	1.371651161713909	4.6819719000706455
>10	5.059695221023491	26.350021080934884
>50	0.04512510974781226	1.151001570244003
>100	0.029871551523199664	2.3388834002256673
>500	0.0044489544821786735	0.9933565384241961
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.355649260255247E-4	2.5365423086235905
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10523	2.530771857759222	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	734	0.17652632743469246	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	593	0.14261595663320523	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	584	0.1404514648799188	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT	568	0.13660347954074295	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	556	0.13371749053636106	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	547	0.13155299878307464	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	539	0.1296290061134867	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	495	0.11904704643075309	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	446	0.10726259132952704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13443898778745653	0.0
2	0.0	0.0	0.0	0.500959591343957	0.0
3	0.0	0.0	0.0	0.8403037984425279	0.0
4	0.0	0.0	0.0	1.1392441594797524	0.0
5	0.0	0.0	0.0	1.9807504533407727	0.0
6	0.0	0.0	0.0	2.852078633580406	0.0
7	0.0	0.0	0.0	3.5476019836364423	0.0
8	0.0	0.0	0.0	4.545913680068879	0.0
9	0.0	0.0	0.0	5.09713757990582	0.0
10	0.0	0.0	0.0	5.885253077185776	0.0
11	0.0	0.0	0.0	6.7962636062356605	0.0
12	0.0	0.0	0.0	7.4804834993578675	0.0
13	0.0	0.0	0.0	7.791448814580017	0.0
14	0.0	0.0	0.0	7.949456712569925	0.0
15	0.0	0.0	0.0	8.158209917220216	0.0
16	0.0	0.0	0.0	8.512465067508092	0.0
17	0.0	0.0	0.0	8.930692974059768	0.0
18	0.0	0.0	0.0	9.420108609386197	0.0
19	0.0	0.0	0.0	9.708467010740689	0.0
20	0.0	0.0	0.0	9.98576245424505	0.0
21	0.0	0.0	0.0	10.308752723652123	0.0
22	0.0	0.0	0.0	10.641122457323437	0.0
23	0.0	0.0	0.0	10.978783170836119	0.0
24	2.4049908369849112E-4	0.0	0.0	11.261369594181847	0.0
25	2.4049908369849112E-4	0.0	0.0	11.51413413114896	0.0
26	2.4049908369849112E-4	0.0	0.0	11.744532253332114	0.0
27	2.4049908369849112E-4	0.0	0.0	11.990081817788274	0.0
28	2.4049908369849112E-4	0.0	0.0	12.232264395072654	0.0
29	2.4049908369849112E-4	0.0	0.0	12.48623142745826	0.0
30	2.4049908369849112E-4	0.0	0.0	12.797677740847808	0.0
31	2.4049908369849112E-4	0.0	0.0	13.056695253991082	0.0
32	2.4049908369849112E-4	0.0	0.0	13.325332730482296	0.0
33	2.4049908369849112E-4	0.0	0.0	13.57352778485914	0.0
34	2.4049908369849112E-4	0.0	0.0	13.806811896046677	0.0
35	2.4049908369849112E-4	0.0	0.0	14.055247449507217	0.0
36	2.4049908369849112E-4	0.0	0.0	14.312340969980905	0.0
37	2.4049908369849112E-4	0.0	0.0	14.570396486789384	0.0
38	2.4049908369849112E-4	0.0	0.0	14.837831467862108	0.0
39	2.4049908369849112E-4	0.0	0.0	15.082419035983474	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGAAG	30	2.1621472E-6	44.999996	1
CGTTTTT	4065	0.0	42.896683	1
TACGAAT	70	0.0	41.785717	12
ATACGAA	55	6.002665E-11	40.909092	19
CATATTA	50	1.0786607E-9	40.5	28
GGTATGC	85	0.0	39.705883	8
TAGGGAT	205	0.0	39.512196	5
TATACGG	40	3.4511322E-7	39.375004	2
CTGACTA	75	0.0	39.0	22
TACTGAC	75	0.0	39.0	20
CGAATAT	75	0.0	39.0	14
GCTACGA	75	0.0	39.0	10
GTAGACG	35	6.2386734E-6	38.57143	1
AAGGGAT	280	0.0	37.76786	5
CATACGA	60	1.546141E-10	37.499996	18
AAGCATA	55	2.741217E-9	36.81818	25
TATGGGC	80	0.0	36.562504	4
AGTGCAG	80	0.0	36.562504	1
AAGCTAC	80	0.0	36.562504	8
GTTTTTT	5010	0.0	36.242516	2
>>END_MODULE