FastQCFastQC Report
Sat 18 Jun 2016
SRR3551561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302958
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.6515754659061652No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC13740.4535282118313429No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC12740.4205203361522059No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT9560.3155552914925501No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC7320.24161764997128313No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC5270.1739515048290522No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4260.14061355039312382No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3810.12576000633751214No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG3730.12311937628318116No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC3620.1194885099584761No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC3550.1171779586609365No Hit
ACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC3520.11618772239056238No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3120.10298457211890756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.0250535E-445.025
TTTAGCG207.0250535E-445.01
TTAGGCG207.0250535E-445.01
TTTACGG253.8839826E-545.02
TTAGCTA207.0250535E-445.01
TCGATTG207.0250535E-445.01
TCGTCGG207.0250535E-445.02
AATTCGT207.0250535E-445.031
ACCGTCG207.0250535E-445.015
CAATCTA207.0250535E-445.044
AATACGG207.0250535E-445.02
ACGGTCT207.0250535E-445.030
CCCTCGT302.1601918E-644.99999614
AGTACGG302.1601918E-644.9999962
TACGGGA900.042.5000044
AACGGGA700.041.7857174
TACGAAT700.041.78571712
CGAATAT650.041.5384614
CGGGATA603.6379788E-1241.2499966
CGTTTTT8450.040.7396471