##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551561_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302958
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29445005578331	33.0	31.0	34.0	30.0	34.0
2	32.410185570277065	34.0	31.0	34.0	30.0	34.0
3	32.41983377233808	34.0	31.0	34.0	30.0	34.0
4	35.89491282620033	37.0	35.0	37.0	35.0	37.0
5	36.00479934512374	37.0	35.0	37.0	35.0	37.0
6	36.03983060358201	37.0	35.0	37.0	35.0	37.0
7	36.17680338528773	37.0	36.0	37.0	35.0	37.0
8	36.09893120498551	37.0	37.0	37.0	35.0	37.0
9	37.94569874372025	39.0	38.0	39.0	35.0	39.0
10	37.69064358755999	39.0	37.0	39.0	35.0	39.0
11	37.62856567577024	39.0	37.0	39.0	35.0	39.0
12	37.58502828774946	39.0	37.0	39.0	35.0	39.0
13	37.543316235253734	39.0	37.0	39.0	35.0	39.0
14	38.84387604882525	40.0	38.0	41.0	35.0	41.0
15	38.911872272724274	40.0	38.0	41.0	35.0	41.0
16	38.92457700407317	40.0	38.0	41.0	35.0	41.0
17	38.85606585731355	40.0	38.0	41.0	35.0	41.0
18	38.7986585599324	40.0	38.0	41.0	35.0	41.0
19	38.74654242502261	40.0	38.0	41.0	35.0	41.0
20	38.65863254972636	40.0	38.0	41.0	35.0	41.0
21	38.554730358663576	40.0	38.0	41.0	35.0	41.0
22	38.54925765287597	40.0	38.0	41.0	35.0	41.0
23	38.520511093947015	40.0	38.0	41.0	35.0	41.0
24	38.45444583077522	40.0	37.0	41.0	35.0	41.0
25	38.34651337809201	40.0	37.0	41.0	34.0	41.0
26	38.35123020352656	40.0	37.0	41.0	34.0	41.0
27	38.317146271100285	40.0	37.0	41.0	34.0	41.0
28	38.26920233167634	40.0	37.0	41.0	34.0	41.0
29	38.221050442635615	40.0	37.0	41.0	34.0	41.0
30	38.01547739290595	40.0	37.0	41.0	34.0	41.0
31	37.99068517748335	40.0	37.0	41.0	34.0	41.0
32	37.85613187306491	40.0	37.0	41.0	34.0	41.0
33	37.723486423860734	40.0	36.0	41.0	33.0	41.0
34	37.63320328230316	40.0	36.0	41.0	33.0	41.0
35	37.53227510083906	40.0	36.0	41.0	33.0	41.0
36	37.40924814660778	40.0	36.0	41.0	33.0	41.0
37	37.35549812185187	40.0	36.0	41.0	33.0	41.0
38	37.21476244231874	40.0	35.0	41.0	33.0	41.0
39	37.231431419536705	40.0	35.0	41.0	33.0	41.0
40	37.136055162761835	40.0	35.0	41.0	32.0	41.0
41	37.07138943351884	40.0	35.0	41.0	32.0	41.0
42	36.9418467246285	39.0	35.0	41.0	32.0	41.0
43	36.92733976326752	39.0	35.0	41.0	32.0	41.0
44	36.898609708276396	39.0	35.0	41.0	32.0	41.0
45	36.80253038374956	39.0	35.0	41.0	32.0	41.0
46	36.731048528178825	39.0	35.0	41.0	31.0	41.0
47	36.68284712732458	39.0	35.0	41.0	31.0	41.0
48	36.64510592227305	39.0	35.0	41.0	31.0	41.0
49	36.62365080308162	39.0	35.0	41.0	31.0	41.0
50	36.55626522488266	39.0	35.0	41.0	31.0	41.0
51	35.63971243538708	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	6.0
11	6.0
12	7.0
13	4.0
14	12.0
15	8.0
16	21.0
17	45.0
18	66.0
19	114.0
20	181.0
21	251.0
22	423.0
23	600.0
24	956.0
25	1564.0
26	2275.0
27	2865.0
28	2871.0
29	3075.0
30	3582.0
31	4263.0
32	5508.0
33	7628.0
34	14456.0
35	21677.0
36	20365.0
37	30310.0
38	59396.0
39	120236.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41201090580213	21.379861234890647	23.44120307105275	13.766924788254478
2	33.01084638794816	24.14823176809987	24.85361007136303	17.98731177258894
3	31.824873414796773	23.62373662355838	27.417661854118393	17.133728107526455
4	29.121528396675444	24.90015117607061	27.494900283207574	18.48342014404637
5	25.21339591626562	28.11577842473214	26.200001320315025	20.47082433868721
6	25.625664283498047	33.5564665729243	25.02756157619208	15.790307567385579
7	75.9290066609893	6.709840968054978	11.840915242376832	5.520237128578879
8	75.61345136949676	6.406828669320499	11.36362136005651	6.616098601126229
9	68.30847840294695	9.025673525703233	14.47065269773368	8.195195373616144
10	36.64105255513966	27.152938691171713	20.742479155526507	15.46352959816212
11	28.28411859069574	23.441533149809544	27.489949101855704	20.784399157639015
12	25.089616382468854	21.790149129582318	32.76988889549046	20.35034559245836
13	23.390370942506884	24.113243419879986	33.87235194317364	18.624033694439493
14	20.05129423880538	27.25031192442517	31.07064345552849	21.627750381240965
15	18.97754804296305	24.88793826206933	35.67755266406565	20.456961030901972
16	21.28941965552981	24.475339816080115	32.180698314617864	22.054542213772205
17	20.707820886063416	24.38225760666495	29.523234243690545	25.38668726358109
18	21.11546815070076	24.53640438608652	32.062530119686556	22.285597343526163
19	22.61897688788545	24.753596208055242	30.007129701146695	22.620297202912614
20	22.96258887370527	25.018319371001922	30.241485618468566	21.777606136824247
21	22.74473689422296	26.084473755438047	30.24478640603648	20.92600294430251
22	21.319786901154618	24.734451640161343	27.916080776873365	26.029680681810678
23	20.20610117574053	25.999973593699455	29.392523056001163	24.40140217455885
24	21.37854091986348	24.95296377715723	30.49003492233247	23.178460380646822
25	20.66590088395091	26.425115032446744	28.957479254550133	23.95150482905221
26	20.547402610262807	26.389136447956485	29.50045880947194	23.563002132308768
27	23.439552677268797	24.560830214089084	29.79422890301626	22.205388205625862
28	21.34487288667076	24.598129113606507	30.489704843575677	23.567293156147056
29	23.10881376296384	24.07396404782181	28.740617511338208	24.07660467787614
30	23.153374395130676	25.246733870701547	29.81997504604599	21.779916688121787
31	25.052977640465013	25.307798440707952	26.140917222849367	23.49830669597766
32	24.46147650829488	27.08626278229986	26.010536113916782	22.441724595488484
33	22.84310036374679	27.916740934386947	26.625472837819103	22.614685864047164
34	22.935522415648375	25.279411667623897	26.958192224664806	24.826873692062925
35	23.638590167613994	25.098198430145434	27.381683269628134	23.88152813261244
36	23.684801193564784	28.365317964866417	26.81988922556922	21.12999161599958
37	23.568943549931014	26.91891285260663	28.33396048297124	21.178183114491116
38	22.170069778649186	29.795549218043426	25.76429736135042	22.27008364195697
39	23.310821962120162	26.72053551977502	26.59807630100542	23.3705662170994
40	22.22915387611484	25.789713425623358	28.825117673076793	23.15601502518501
41	20.26650558823335	26.97271568996363	27.37805240330343	25.382726318499593
42	21.649865657945984	27.084942467272693	28.071217792565307	23.193974082216016
43	23.579836148905127	24.465437453376378	29.26445249836611	22.690273899352388
44	23.306200859525084	24.538384858627268	27.784049274156814	24.371365007690834
45	23.73926418843536	23.64354134896586	27.44637870595924	25.170815756639538
46	23.533625122954337	25.172796229180282	28.344853081945352	22.94872556592003
47	21.498359508578744	25.185669300695146	30.451415707787877	22.86455548293823
48	20.805854276830452	25.279411667623897	29.343341321239247	24.571392734306404
49	22.34468144099182	24.260458545408934	30.682470837541835	22.712389176057407
50	21.64821526416203	23.196614712270346	30.703595877976486	24.451574145591138
51	21.370619029700485	23.398622911426667	27.58501178381162	27.645746275061228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	471.5
2	721.0
3	1954.0
4	3187.0
5	2126.5
6	1066.0
7	1002.0
8	938.0
9	962.0
10	986.0
11	977.0
12	968.0
13	951.0
14	934.0
15	875.0
16	816.0
17	844.5
18	873.0
19	860.5
20	848.0
21	879.0
22	910.0
23	1071.0
24	1232.0
25	1565.0
26	2354.0
27	2810.0
28	3138.0
29	3466.0
30	4168.0
31	4870.0
32	5317.0
33	5764.0
34	6408.5
35	7053.0
36	7749.0
37	8445.0
38	8876.5
39	9308.0
40	10762.0
41	12216.0
42	14038.5
43	15861.0
44	17450.5
45	19040.0
46	22599.0
47	26158.0
48	29938.5
49	33719.0
50	32843.5
51	31968.0
52	27185.0
53	22402.0
54	19572.5
55	16743.0
56	15130.5
57	13518.0
58	12923.5
59	12329.0
60	11418.0
61	10507.0
62	9726.5
63	8946.0
64	8071.0
65	7196.0
66	6214.0
67	5232.0
68	4330.5
69	3429.0
70	2993.0
71	2557.0
72	2078.0
73	1599.0
74	1278.0
75	761.5
76	566.0
77	464.5
78	363.0
79	272.0
80	181.0
81	146.5
82	112.0
83	75.0
84	38.0
85	21.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.72666529990265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4666401855624	24.103314536470357
2	9.875913704993337	6.661632721180635
3	3.6714631083799083	3.714786222518081
4	1.9968594634556212	2.693896430996437
5	1.4451271868294042	2.4369660472992596
6	1.125317492456252	2.277192385451864
7	0.9704112537298047	2.291011489046282
8	0.933433673856988	2.51852840782665
9	0.7925191675663359	2.405612583744054
>10	7.577402683364237	43.25248955321568
>50	0.08494846057576289	1.964061163529755
>100	0.053967257306955244	3.37601452100807
>500	0.002998180961497514	0.7465926681342727
>1k	0.002998180961497514	1.5579012695786043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1974	0.6515754659061652	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1374	0.4535282118313429	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1274	0.4205203361522059	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	956	0.3155552914925501	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC	732	0.24161764997128313	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	527	0.1739515048290522	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	426	0.14061355039312382	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	381	0.12576000633751214	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	373	0.12311937628318116	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	362	0.1194885099584761	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	355	0.1171779586609365	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	352	0.11618772239056238	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	312	0.10298457211890756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32545765419629125	0.0
2	0.0	0.0	0.0	1.5302451164847932	0.0
3	0.0	0.0	0.0	2.087748136705418	0.0
4	0.0	0.0	0.0	2.725790373583137	0.0
5	0.0	0.0	0.0	4.591395506967962	0.0
6	0.0	0.0	0.0	5.594174770100146	0.0
7	0.0	0.0	0.0	6.626331042586761	0.0
8	0.0	0.0	0.0	8.232164194376779	0.0
9	0.0	0.0	0.0	8.883409581526152	0.0
10	0.0	0.0	0.0	10.249275477128842	0.0
11	0.0	0.0	0.0	12.01618706223305	0.0
12	0.0	0.0	0.0	13.431894850111236	0.0
13	0.0	0.0	0.0	13.9649720423293	0.0
14	0.0	0.0	0.0	14.173581816621446	0.0
15	0.0	0.0	0.0	14.605984988018141	0.0
16	0.0	0.0	0.0	15.434482667564481	0.0
17	0.0	0.0	0.0	16.440562718264577	0.0
18	0.0	0.0	0.0	17.52289096178348	0.0
19	0.0	0.0	0.0	18.135517134388266	0.0
20	0.0	0.0	0.0	18.722397163963322	0.0
21	0.0	0.0	0.0	19.354167904462006	0.0
22	0.0	0.0	0.0	20.02224730820774	0.0
23	0.0	0.0	0.0	20.715742776226406	0.0
24	0.0	0.0	0.0	21.281827844123608	0.0
25	0.0	0.0	0.0	21.799061256015687	0.0
26	0.0	0.0	0.0	22.257540649198898	0.0
27	3.3007875679137044E-4	0.0	0.0	22.72064114497719	0.0
28	3.3007875679137044E-4	0.0	0.0	23.200575657351845	0.0
29	3.3007875679137044E-4	0.0	0.0	23.694043398754943	0.0
30	3.3007875679137044E-4	0.0	0.0	24.246925316380487	0.0
31	3.3007875679137044E-4	0.0	0.0	24.739732900270006	0.0
32	3.3007875679137044E-4	0.0	0.0	25.200522844750758	0.0
33	6.601575135827409E-4	0.0	0.0	25.65834208042039	0.0
34	6.601575135827409E-4	0.0	0.0	26.093715960628206	0.0
35	6.601575135827409E-4	0.0	0.0	26.586853623274514	0.0
36	6.601575135827409E-4	0.0	0.0	27.044672858944143	0.0
37	6.601575135827409E-4	0.0	0.0	27.478066266611215	0.0
38	6.601575135827409E-4	0.0	0.0	27.897926445249837	0.0
39	6.601575135827409E-4	0.0	0.0	28.31547607259092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0250535E-4	45.0	25
TTTAGCG	20	7.0250535E-4	45.0	1
TTAGGCG	20	7.0250535E-4	45.0	1
TTTACGG	25	3.8839826E-5	45.0	2
TTAGCTA	20	7.0250535E-4	45.0	1
TCGATTG	20	7.0250535E-4	45.0	1
TCGTCGG	20	7.0250535E-4	45.0	2
AATTCGT	20	7.0250535E-4	45.0	31
ACCGTCG	20	7.0250535E-4	45.0	15
CAATCTA	20	7.0250535E-4	45.0	44
AATACGG	20	7.0250535E-4	45.0	2
ACGGTCT	20	7.0250535E-4	45.0	30
CCCTCGT	30	2.1601918E-6	44.999996	14
AGTACGG	30	2.1601918E-6	44.999996	2
TACGGGA	90	0.0	42.500004	4
AACGGGA	70	0.0	41.785717	4
TACGAAT	70	0.0	41.785717	12
CGAATAT	65	0.0	41.53846	14
CGGGATA	60	3.6379788E-12	41.249996	6
CGTTTTT	845	0.0	40.739647	1
>>END_MODULE