FastQCFastQC Report
Sat 18 Jun 2016
SRR3551560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589345
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60021.0184187530224231No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC11200.1900414867352739TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT6850.11623073072648449TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6630.11249777295132732No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5980.1014685795247266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTC207.030486E-445.011
AAACGCG253.8884842E-545.01
AACGCGT207.030486E-445.025
CGATCAA253.8884842E-545.010
ATACCGG207.030486E-445.02
CGTTTTT22650.040.629141
ATTAGCG501.0804797E-940.51
TACGAAT501.0804797E-940.512
ACGCGCA451.9255822E-840.00000417
GCGTTTA403.4544064E-739.37532
CGTTTAC403.4544064E-739.37533
TCGTTTA356.243108E-638.57142638
GTAAGCG356.243108E-638.5714261
CACAACG700.038.57142612
CCAGTCG356.243108E-638.57142628
CGTAAGG900.037.5000042
CTATGCG301.139247E-437.4999961
TATTGCG301.139247E-437.4999961
CAAGCCG301.139247E-437.4999961
TTACGAG301.139247E-437.4999961