##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589345
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46601396465568	33.0	31.0	34.0	31.0	34.0
2	32.58762863857333	34.0	31.0	34.0	31.0	34.0
3	32.57452765358152	34.0	31.0	34.0	31.0	34.0
4	36.076221907371746	37.0	35.0	37.0	35.0	37.0
5	36.16714318438266	37.0	35.0	37.0	35.0	37.0
6	36.18252127361732	37.0	37.0	37.0	35.0	37.0
7	36.30887680390943	37.0	37.0	37.0	35.0	37.0
8	36.25667817661981	37.0	37.0	37.0	35.0	37.0
9	38.1008713062807	39.0	39.0	39.0	37.0	39.0
10	37.845581111233656	39.0	38.0	39.0	35.0	39.0
11	37.78385156402447	39.0	38.0	39.0	35.0	39.0
12	37.73650747864154	39.0	37.0	39.0	35.0	39.0
13	37.7175355691488	39.0	38.0	39.0	35.0	39.0
14	39.0615479897174	40.0	39.0	41.0	35.0	41.0
15	39.1450644359416	40.0	39.0	41.0	35.0	41.0
16	39.15656533948706	40.0	39.0	41.0	35.0	41.0
17	39.088956383782	40.0	38.0	41.0	35.0	41.0
18	38.999056579762275	40.0	38.0	41.0	35.0	41.0
19	38.90737174320644	40.0	38.0	41.0	35.0	41.0
20	38.78725364599683	40.0	38.0	41.0	35.0	41.0
21	38.685065623700886	40.0	38.0	41.0	35.0	41.0
22	38.69048010927386	40.0	38.0	41.0	35.0	41.0
23	38.63768251194122	40.0	38.0	41.0	35.0	41.0
24	38.569006269672265	40.0	38.0	41.0	35.0	41.0
25	38.47120786636011	40.0	37.0	41.0	35.0	41.0
26	38.4700523462488	40.0	37.0	41.0	35.0	41.0
27	38.47221915855738	40.0	37.0	41.0	35.0	41.0
28	38.37625329815303	40.0	37.0	41.0	35.0	41.0
29	38.33615624125088	40.0	37.0	41.0	35.0	41.0
30	38.19774495414401	40.0	37.0	41.0	34.0	41.0
31	38.13527390577675	40.0	37.0	41.0	34.0	41.0
32	37.989332224757995	40.0	37.0	41.0	34.0	41.0
33	37.872651842299504	40.0	37.0	41.0	34.0	41.0
34	37.71292706309547	40.0	37.0	41.0	34.0	41.0
35	37.610014507631355	40.0	36.0	41.0	33.0	41.0
36	37.53983659825739	40.0	36.0	41.0	33.0	41.0
37	37.482917476181186	40.0	36.0	41.0	33.0	41.0
38	37.330972520340374	40.0	36.0	41.0	33.0	41.0
39	37.317491452375094	40.0	36.0	41.0	33.0	41.0
40	37.23902467994129	40.0	35.0	41.0	33.0	41.0
41	37.16826137491622	40.0	35.0	41.0	33.0	41.0
42	37.08198932713436	40.0	35.0	41.0	33.0	41.0
43	37.067670040468656	40.0	35.0	41.0	33.0	41.0
44	37.01518465414995	40.0	35.0	41.0	33.0	41.0
45	36.904755279165855	39.0	35.0	41.0	32.0	41.0
46	36.851937320245355	39.0	35.0	41.0	32.0	41.0
47	36.81535603084781	39.0	35.0	41.0	32.0	41.0
48	36.764070281414114	39.0	35.0	41.0	32.0	41.0
49	36.71075516038992	39.0	35.0	41.0	32.0	41.0
50	36.63827978518525	39.0	35.0	41.0	32.0	41.0
51	35.84463938779493	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	13.0
10	13.0
11	11.0
12	9.0
13	15.0
14	22.0
15	20.0
16	38.0
17	55.0
18	96.0
19	208.0
20	345.0
21	618.0
22	838.0
23	1265.0
24	1867.0
25	3068.0
26	4373.0
27	5655.0
28	5900.0
29	5678.0
30	6047.0
31	7209.0
32	8908.0
33	12424.0
34	25979.0
35	39186.0
36	37295.0
37	56496.0
38	109739.0
39	255625.0
40	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78867216995138	20.151184789893865	24.81483681035726	14.245306229797485
2	33.990956061390186	23.905013192612138	24.97789919317208	17.126131552825594
3	32.16723650832704	22.95972647600302	28.05453511949707	16.81850189617287
4	28.023314018104845	25.224613766130194	27.85855483630132	18.893517379463642
5	26.15598673103191	28.81351330714607	25.782181913819578	19.248318048002446
6	26.154459611942073	33.8277239986765	25.028972842732184	14.988843546649248
7	79.0523377648067	6.040774079698648	10.63587542101825	4.27101273447641
8	80.72232732949291	5.137058938312872	9.741832033868107	4.398781698326108
9	73.31257582570481	8.11799540167474	12.410048443611128	6.159380329009323
10	36.30352340310005	30.731574883981374	18.60590995087767	14.35899176204091
11	28.178740805470483	24.658391943598403	27.96104149521927	19.201825755711848
12	25.492538326447157	21.695102189719094	31.967692947255006	20.844666536578742
13	24.34550221008068	22.784956180166116	33.07790852556652	19.79163308418668
14	20.987537011427943	26.45207815456142	31.032926384375877	21.527458449634764
15	20.251635290025366	24.522648024501777	34.78030695093706	20.4454097345358
16	22.810747524794476	24.25930482145433	32.37628214373584	20.553665510015357
17	22.9917959768896	23.178443865647456	30.641644537579854	23.18811561988309
18	23.401912292460274	23.454513061110216	31.123874810170616	22.019699836258898
19	24.444086231324604	24.772077475841826	29.749297949418423	21.034538343415146
20	24.66891209732839	24.500250277850835	29.38686168543044	21.443975939390338
21	24.635994196947458	25.17337043667122	29.487990905157417	20.7026444612239
22	23.06883065097693	23.851054984771228	28.960965139264776	24.119149224987062
23	21.79215909187318	25.35900024603585	30.17892745335924	22.669913208731725
24	21.76551934775047	25.00402989759818	30.540854677650614	22.689596077000736
25	22.558094155375883	25.756560249090093	29.13811095368587	22.547234641848153
26	21.15467171181566	26.251686193995027	28.994052719544577	23.599589374644733
27	22.43117359102054	25.548193333276775	29.872655235897476	22.147977839805208
28	20.745064435941597	26.375552520170697	30.360145585353234	22.519237458534473
29	21.586337374542925	26.053500072113955	29.169162375179226	23.191000178163893
30	22.57048078799345	25.679016535306143	29.560613901874117	22.18988877482629
31	23.674248530147874	26.766495007168977	26.974692243083425	22.584564219599727
32	22.567596229712645	27.233284408962493	27.281473500241795	22.917645861083066
33	22.822285757917687	25.899600403838157	27.49119785524608	23.786915982998075
34	21.720893534347454	26.75003605697851	27.670719188251365	23.858351220422673
35	21.666426286809934	26.23471820410795	27.902841289906593	24.196014219175527
36	22.814650162468503	27.13894238519034	27.225309453715568	22.821097998625593
37	21.80284892550204	27.79136159634849	27.48576809848221	22.920021379667258
38	21.29601506757502	27.80544502795476	27.185604357379805	23.712935547090414
39	22.457983015042124	25.338129618474746	27.3615624125088	24.842324953974327
40	22.49633067218692	25.12110902781902	29.700430138543638	22.682130161450424
41	20.184272370173666	25.930991185129255	28.73070951649713	25.15402692819995
42	20.671423359831678	27.170842206178047	28.340615428993203	23.81711900499707
43	21.814217478726384	25.231061602287287	28.749034945575175	24.20568597341116
44	21.9453800405535	25.95830964884745	28.40203955238443	23.69427075821463
45	21.87445384282551	25.11398247206645	27.977161085611996	25.034402599496055
46	22.446784141716652	26.496194928267826	27.72009603882276	23.336924891192766
47	20.730641644537577	26.10168916339326	29.481882428798073	23.68578676327109
48	20.67668343669667	25.55837412720902	29.329849239409857	24.435093196684456
49	21.077637037728326	25.239545597230823	28.992695280353615	24.69012208468724
50	20.239078977508928	24.795662981784865	30.11241293300189	24.852845107704315
51	21.065759444807373	24.848433430333674	28.18993967879595	25.895867446063004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	347.0
1	815.5
2	1284.0
3	5345.0
4	9406.0
5	5981.0
6	2556.0
7	2464.5
8	2373.0
9	2294.0
10	2215.0
11	2147.5
12	2080.0
13	2029.0
14	1978.0
15	1870.5
16	1763.0
17	1743.0
18	1723.0
19	1789.0
20	1855.0
21	1963.5
22	2072.0
23	2297.0
24	2522.0
25	3350.5
26	4719.0
27	5259.0
28	6371.5
29	7484.0
30	8608.5
31	9733.0
32	11076.5
33	12420.0
34	13873.5
35	15327.0
36	15753.0
37	16179.0
38	18339.5
39	20500.0
40	22999.5
41	25499.0
42	27562.0
43	29625.0
44	32574.0
45	35523.0
46	40921.5
47	46320.0
48	50237.5
49	54155.0
50	51938.0
51	49721.0
52	45291.0
53	40861.0
54	36712.0
55	32563.0
56	30840.0
57	29117.0
58	28146.0
59	27175.0
60	25663.0
61	24151.0
62	21903.0
63	19655.0
64	18092.5
65	16530.0
66	13870.0
67	11210.0
68	9779.0
69	8348.0
70	6786.0
71	5224.0
72	4780.0
73	4336.0
74	3520.0
75	2187.0
76	1670.0
77	1293.0
78	916.0
79	681.0
80	446.0
81	329.0
82	212.0
83	159.0
84	106.0
85	62.5
86	19.0
87	11.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	589345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.471759275703512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89790852931888	21.189578526026363
2	8.467245597935516	4.990892479812316
3	3.144891153542923	2.7805642507651966
4	1.811589973632486	2.135629744366988
5	1.26666808158646	1.8665468391366662
6	1.0877001206993067	1.9233861672842176
7	0.955925695055999	1.9720968379104975
8	0.879391039571258	2.073376082196382
9	0.7386886942557513	1.959340983911033
>10	9.604689036445242	52.98181686562907
>50	0.09725398046468127	1.96423149146371
>100	0.04457474104631215	2.5287795680079093
>500	0.002315570963444787	0.418676633375483
>1k	5.788927408611967E-4	0.19108306005727016
>5k	5.788927408611967E-4	1.0240004700569068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6002	1.0184187530224231	No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	1120	0.1900414867352739	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	685	0.11623073072648449	TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	663	0.11249777295132732	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	598	0.1014685795247266	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6967989887078027E-4	0.0	0.0	0.11945464880502932	0.0
2	1.6967989887078027E-4	0.0	0.0	0.5024221805563804	0.0
3	1.6967989887078027E-4	0.0	0.0	0.720460850605333	0.0
4	1.6967989887078027E-4	0.0	0.0	1.0382713011903044	0.0
5	1.6967989887078027E-4	0.0	0.0	1.8939670311956494	0.0
6	1.6967989887078027E-4	0.0	0.0	2.5406171257921932	0.0
7	1.6967989887078027E-4	0.0	0.0	3.1292366949749297	0.0
8	1.6967989887078027E-4	0.0	0.0	4.147316088199611	0.0
9	1.6967989887078027E-4	0.0	0.0	4.644308511992127	0.0
10	1.6967989887078027E-4	0.0	0.0	5.453681629605748	0.0
11	1.6967989887078027E-4	0.0	0.0	6.368256284519254	0.0
12	1.6967989887078027E-4	0.0	0.0	7.1570981343695115	0.0
13	1.6967989887078027E-4	0.0	0.0	7.458619314662888	0.0
14	1.6967989887078027E-4	0.0	0.0	7.593684514164029	0.0
15	1.6967989887078027E-4	0.0	0.0	7.83072733288651	0.0
16	1.6967989887078027E-4	0.0	0.0	8.290559858826324	0.0
17	1.6967989887078027E-4	0.0	0.0	8.836589773392495	0.0
18	1.6967989887078027E-4	0.0	0.0	9.506146654336595	0.0
19	1.6967989887078027E-4	0.0	0.0	9.856026605808143	0.0
20	1.6967989887078027E-4	0.0	0.0	10.248156852098516	0.0
21	1.6967989887078027E-4	0.0	0.0	10.691869787645606	0.0
22	1.6967989887078027E-4	0.0	0.0	11.175966539123943	0.0
23	1.6967989887078027E-4	0.0	0.0	11.628163469614572	0.0
24	1.6967989887078027E-4	0.0	0.0	11.998744368748357	0.0
25	1.6967989887078027E-4	0.0	0.0	12.344212642849264	0.0
26	1.6967989887078027E-4	0.0	0.0	12.657611416063595	0.0
27	1.6967989887078027E-4	0.0	0.0	12.99340793592887	0.0
28	1.6967989887078027E-4	0.0	0.0	13.337688450737684	0.0
29	1.6967989887078027E-4	0.0	0.0	13.695713037355029	0.0
30	1.6967989887078027E-4	0.0	0.0	14.106168712723447	0.0
31	1.6967989887078027E-4	0.0	0.0	14.473016654082075	0.0
32	1.6967989887078027E-4	0.0	0.0	14.83324707938474	0.0
33	1.6967989887078027E-4	0.0	0.0	15.184654149946127	0.0
34	1.6967989887078027E-4	0.0	0.0	15.539624498383798	0.0
35	1.6967989887078027E-4	0.0	0.0	15.94431105719061	0.0
36	1.6967989887078027E-4	0.0	0.0	16.299451085527153	0.0
37	1.6967989887078027E-4	0.0	0.0	16.668674545469973	0.0
38	1.6967989887078027E-4	0.0	0.0	17.033316648143277	0.0
39	1.6967989887078027E-4	0.0	0.0	17.415944820096886	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTC	20	7.030486E-4	45.0	11
AAACGCG	25	3.8884842E-5	45.0	1
AACGCGT	20	7.030486E-4	45.0	25
CGATCAA	25	3.8884842E-5	45.0	10
ATACCGG	20	7.030486E-4	45.0	2
CGTTTTT	2265	0.0	40.62914	1
ATTAGCG	50	1.0804797E-9	40.5	1
TACGAAT	50	1.0804797E-9	40.5	12
ACGCGCA	45	1.9255822E-8	40.000004	17
GCGTTTA	40	3.4544064E-7	39.375	32
CGTTTAC	40	3.4544064E-7	39.375	33
TCGTTTA	35	6.243108E-6	38.571426	38
GTAAGCG	35	6.243108E-6	38.571426	1
CACAACG	70	0.0	38.571426	12
CCAGTCG	35	6.243108E-6	38.571426	28
CGTAAGG	90	0.0	37.500004	2
CTATGCG	30	1.139247E-4	37.499996	1
TATTGCG	30	1.139247E-4	37.499996	1
CAAGCCG	30	1.139247E-4	37.499996	1
TTACGAG	30	1.139247E-4	37.499996	1
>>END_MODULE