##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339098
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.364393184271215	33.0	31.0	34.0	31.0	34.0
2	32.48526974502946	34.0	31.0	34.0	31.0	34.0
3	32.46966953506066	34.0	31.0	34.0	31.0	34.0
4	35.95356504609287	37.0	35.0	37.0	35.0	37.0
5	36.07900665884198	37.0	35.0	37.0	35.0	37.0
6	36.117202106765596	37.0	36.0	37.0	35.0	37.0
7	36.235651640528694	37.0	37.0	37.0	35.0	37.0
8	36.16142531067715	37.0	37.0	37.0	35.0	37.0
9	38.01023008097954	39.0	39.0	39.0	35.0	39.0
10	37.751387504497224	39.0	37.0	39.0	35.0	39.0
11	37.692124400615754	39.0	37.0	39.0	35.0	39.0
12	37.604878235790245	39.0	37.0	39.0	35.0	39.0
13	37.54666497590667	39.0	37.0	39.0	35.0	39.0
14	38.816828763366345	40.0	38.0	41.0	35.0	41.0
15	38.89481211921038	40.0	38.0	41.0	35.0	41.0
16	38.9322998071354	40.0	38.0	41.0	35.0	41.0
17	38.88971624722057	40.0	38.0	41.0	35.0	41.0
18	38.7986481784027	40.0	38.0	41.0	35.0	41.0
19	38.707267515585464	40.0	37.0	41.0	35.0	41.0
20	38.58765607582469	40.0	37.0	41.0	35.0	41.0
21	38.48240921503518	40.0	37.0	41.0	35.0	41.0
22	38.467077364065844	40.0	37.0	41.0	35.0	41.0
23	38.429159122141684	40.0	37.0	41.0	35.0	41.0
24	38.345097877309804	40.0	37.0	41.0	35.0	41.0
25	38.23527711752945	40.0	36.0	41.0	34.0	41.0
26	38.23640363552719	40.0	36.0	41.0	34.0	41.0
27	38.25148777049702	40.0	36.0	41.0	35.0	41.0
28	38.18736471462527	40.0	36.0	41.0	34.0	41.0
29	38.09583955080832	40.0	36.0	41.0	34.0	41.0
30	37.95238544609523	40.0	36.0	41.0	34.0	41.0
31	37.89421937021156	40.0	36.0	41.0	34.0	41.0
32	37.67263151065474	40.0	35.0	41.0	34.0	41.0
33	37.56344183687312	40.0	35.0	41.0	33.0	41.0
34	37.39708874720582	40.0	35.0	41.0	33.0	41.0
35	37.25328371149343	40.0	35.0	41.0	33.0	41.0
36	37.17212133365576	40.0	35.0	41.0	33.0	41.0
37	37.09266937581466	40.0	35.0	41.0	33.0	41.0
38	36.88442574123115	39.0	35.0	41.0	32.0	41.0
39	36.90680570218639	39.0	35.0	41.0	32.0	41.0
40	36.82916148134168	39.0	35.0	41.0	32.0	41.0
41	36.713616712572765	39.0	35.0	41.0	32.0	41.0
42	36.584303062831395	39.0	35.0	41.0	31.0	41.0
43	36.62226553975547	39.0	35.0	41.0	31.0	41.0
44	36.58799521082401	39.0	35.0	41.0	31.0	41.0
45	36.51059280797882	39.0	35.0	41.0	31.0	41.0
46	36.456301718087396	39.0	35.0	41.0	31.0	41.0
47	36.37851004724298	39.0	35.0	41.0	31.0	41.0
48	36.316306790367385	39.0	35.0	41.0	31.0	41.0
49	36.253959032492084	39.0	35.0	41.0	31.0	41.0
50	36.18836147662328	38.0	35.0	41.0	31.0	41.0
51	35.21868604356263	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	11.0
11	4.0
12	8.0
13	14.0
14	9.0
15	21.0
16	18.0
17	23.0
18	53.0
19	107.0
20	190.0
21	321.0
22	488.0
23	770.0
24	1216.0
25	1933.0
26	2809.0
27	3431.0
28	3634.0
29	3586.0
30	3934.0
31	4721.0
32	5966.0
33	8502.0
34	18214.0
35	27441.0
36	23294.0
37	34149.0
38	66801.0
39	127260.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.355006517289986	19.719962960560075	24.514447150971105	14.410583371178834
2	34.13467493173065	24.218367551563265	24.678706450642586	16.968251066063498
3	31.930002536139995	23.676046452647906	28.26587004346826	16.128080967743838
4	29.537183940925633	23.684008752631982	27.8651009442698	18.913706362172586
5	25.1590985496818	28.19922264360156	26.39738364720523	20.24429515951141
6	26.214545647570908	32.89668473420663	25.471987449056027	15.416782169166435
7	75.79106924841787	6.997387186005226	12.104465375791069	5.1070781897858435
8	75.56340644887318	6.1982081876035835	12.298214675403571	5.940170688119658
9	68.41797946316403	8.573037882853924	14.796312570407375	8.21267008357466
10	38.473243723053514	23.938212552123574	21.618234256763532	15.970309468059382
11	30.336068039327863	23.078579053842844	26.50826604698347	20.077086859845828
12	27.54395484491209	20.40531055918938	32.48294003503412	19.56779456086441
13	24.2858996514282	22.6436015547128	33.41423423317153	19.656264560687468
14	20.015747659968504	27.314522645370953	31.10487233779026	21.564857356870284
15	19.91489186017022	23.562804852874393	36.43666432712667	20.08563895982872
16	21.858872656282255	22.92552595414895	33.094857533810284	22.120743855758512
17	21.391456157217085	22.869789854260418	29.81232564037535	25.926428348147144
18	21.38201935723596	24.060890951878218	31.692018236615965	22.865071454269856
19	24.01665595196669	24.691682050616638	28.99486284201027	22.2967991554064
20	25.116632949766732	24.290028251419944	29.34137034131726	21.251968457496062
21	23.92199305215601	24.926717350146564	29.75983344048033	21.391456157217085
22	22.218355755563287	24.030811151938376	27.835316044329367	25.91551704816897
23	21.835280656329438	25.00516074998968	29.082153241835695	24.077405351845186
24	22.965632354068735	23.765106252469785	29.630372340739253	23.638889052722224
25	22.01575945596848	25.22663064954674	28.69052604261895	24.067083851865835
26	20.25933505948133	25.928787548142424	29.971571640056855	23.840305752319388
27	22.97418445405163	24.6356510507287	29.111348341777305	23.278816153442367
28	20.972993058054016	24.143168051713666	31.012863537974273	23.87097535225805
29	22.084766055830467	23.72146105255708	30.454912739090172	23.738860152522278
30	23.468436853063128	23.985101652029797	28.828244342343513	23.718217152563565
31	26.345186347309628	24.057941951884114	25.844446148311107	23.752425552495147
32	24.010757951978484	25.259364549481273	28.639508342720983	22.09036915581926
33	25.340167149319665	24.395602451208795	26.21867424756265	24.045556151908887
34	24.354316451291368	23.628567552742865	29.00695374198609	23.010162253979676
35	23.60792455278415	22.490843355018313	29.735061840529873	24.16617025166766
36	25.580215748839567	25.12577484974845	27.189190145621623	22.10481925579036
37	24.492034751015932	25.147302549705397	29.23432164153136	21.126341057747318
38	23.45457655309085	26.14111554771777	27.947378044105243	22.45692985508614
39	25.63949064872102	23.464603153070794	28.7064506425871	22.189455555621088
40	25.037599749924798	21.91018525617963	31.17004523765991	21.882169756235662
41	21.140201357719597	24.940282750119437	29.10427074179146	24.81524515036951
42	21.76804345646391	25.285315749429373	29.529811440940378	23.416829353166342
43	23.760682752478633	23.28206005343588	29.025237541949522	23.93201965213596
44	23.817893352364212	22.718801054562398	28.74036414251927	24.722941450554117
45	23.791942152416116	22.551592754896816	28.611197942777604	25.045267149909467
46	24.840016750319965	23.485541053028918	29.148210841703577	22.526231354947537
47	21.993347056013306	23.882181552235636	31.14291443771417	22.981556954036886
48	22.064712855870575	22.951772054096455	30.28416563943167	24.6993494506013
49	22.3256993553486	22.678694654642612	31.490601537018797	23.50500445298999
50	21.815522356368955	21.949996756100006	31.444007337111984	24.790473550419055
51	21.620298556759405	22.729712354540577	28.37144424325711	27.27854484544291
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	529.5
2	840.0
3	2927.5
4	5015.0
5	3245.0
6	1475.0
7	1410.5
8	1346.0
9	1391.0
10	1436.0
11	1406.0
12	1376.0
13	1436.5
14	1497.0
15	1400.0
16	1303.0
17	1239.0
18	1175.0
19	1103.0
20	1031.0
21	1135.5
22	1240.0
23	1219.0
24	1198.0
25	1580.5
26	2270.5
27	2578.0
28	2984.0
29	3390.0
30	3917.5
31	4445.0
32	4843.5
33	5242.0
34	5807.0
35	6372.0
36	7103.5
37	7835.0
38	8286.5
39	8738.0
40	10216.0
41	11694.0
42	13634.0
43	15574.0
44	17288.5
45	19003.0
46	23492.0
47	27981.0
48	31492.0
49	35003.0
50	34085.5
51	33168.0
52	29119.5
53	25071.0
54	22025.5
55	18980.0
56	17865.0
57	16750.0
58	16361.5
59	15973.0
60	15514.5
61	15056.0
62	13677.5
63	12299.0
64	11130.0
65	9961.0
66	8535.0
67	7109.0
68	6258.0
69	5407.0
70	4605.0
71	3803.0
72	3199.0
73	2595.0
74	2125.5
75	1287.0
76	918.0
77	799.0
78	680.0
79	565.5
80	451.0
81	313.0
82	175.0
83	110.0
84	45.0
85	26.5
86	8.0
87	12.0
88	16.0
89	9.0
90	2.0
91	1.5
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.969479208233224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.22814182706703	25.607499830791742
2	9.604450929718663	6.717252941865862
3	3.390629342703176	3.557056269074526
4	1.7800565765129097	2.4899060576738803
5	1.2534778723898956	2.1916734198259435
6	1.0131352937971712	2.1257288154940457
7	0.8212745153968484	2.0103679463295334
8	0.7832428325825258	2.1911675159193864
9	0.7418397541437293	2.334757487253299
>10	7.243494429515253	43.45181177204541
>50	0.08618178234714825	2.0150639757994604
>100	0.049005327217005866	3.069574727944369
>500	0.00253475830432789	0.5596086759771522
>1k	0.00253475830432789	1.6785305640053865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3161	0.9321788981356423	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1282	0.3780617992438764	TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1238	0.3650861992698276	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	862	0.2542037994915924	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	522	0.1539377996921244	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	510	0.150398999699202	TruSeq Adapter, Index 21 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	398	0.1173701997652596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCG	362	0.10675379978649241	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	347	0.1023302997953394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2612813994774372	0.0
2	0.0	0.0	0.0	1.2772118974455762	0.0
3	0.0	0.0	0.0	1.744333496511333	0.0
4	0.0	0.0	0.0	2.307002695385995	0.0
5	0.0	0.0	0.0	4.071979191856042	0.0
6	0.0	0.0	0.0	5.012415289975169	0.0
7	0.0	0.0	0.0	5.981161788037676	0.0
8	0.0	0.0	0.0	7.411131885177737	0.0
9	0.0	0.0	0.0	8.028062683943874	0.0
10	0.0	0.0	0.0	9.366318881267363	0.0
11	0.0	0.0	0.0	11.084111377831777	0.0
12	0.0	0.0	0.0	12.4869506750261	0.0
13	0.0	0.0	0.0	13.024553373950893	0.0
14	0.0	0.0	0.0	13.259588673480822	0.0
15	0.0	0.0	0.0	13.715504072568992	0.0
16	0.0	0.0	0.0	14.560392570879214	0.0
17	0.0	0.0	0.0	15.539165668921669	0.0
18	0.0	0.0	0.0	16.671581666656838	0.0
19	0.0	0.0	0.0	17.288807365422386	0.0
20	0.0	0.0	0.0	17.90013506419973	0.0
21	0.0	0.0	0.0	18.647116762705767	0.0
22	0.0	0.0	0.0	19.366377861267246	0.0
23	0.0	0.0	0.0	20.05378975989242	0.0
24	0.0	0.0	0.0	20.622946758754107	0.0
25	0.0	0.0	0.0	21.13223905773552	0.0
26	0.0	0.0	0.0	21.609682156780636	0.0
27	0.0	0.0	0.0	22.05734035588532	0.0
28	0.0	0.0	0.0	22.536552854926896	0.0
29	0.0	0.0	0.0	22.995122354009755	0.0
30	0.0	0.0	0.0	23.514146352971707	0.0
31	0.0	0.0	0.0	24.006924251986153	0.0
32	0.0	0.0	0.0	24.510318550979363	0.0
33	0.0	0.0	0.0	24.988056550023888	0.0
34	0.0	0.0	0.0	25.451344449097313	0.0
35	0.0	0.0	0.0	25.95031524809937	0.0
36	0.0	0.0	0.0	26.40829494718341	0.0
37	0.0	0.0	0.0	26.86509504626981	0.0
38	0.0	0.0	0.0	27.325139045349722	0.0
39	0.0	0.0	0.0	27.76129614447741	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAC	30	2.1609576E-6	45.000004	17
ACGGGCG	30	2.1609576E-6	45.000004	5
GGCGGTA	30	2.1609576E-6	45.000004	16
CGGTACT	30	2.1609576E-6	45.000004	18
TTAGGCG	45	3.8380676E-10	45.0	1
CTCCATA	20	7.026245E-4	45.0	35
ATTCGAC	20	7.026245E-4	45.0	42
GATCAAC	25	3.8849692E-5	45.0	9
CGATTCG	20	7.026245E-4	45.0	10
ATATGCG	25	3.8849692E-5	45.0	1
TCGATAA	45	3.8380676E-10	45.0	1
CGTTCAT	50	2.1827873E-11	45.0	17
CCATATG	20	7.026245E-4	45.0	28
TTGCGAG	35	1.2085366E-7	45.0	1
GTATAAG	20	7.026245E-4	45.0	1
CAATGCG	20	7.026245E-4	45.0	1
GAATTGA	20	7.026245E-4	45.0	10
CGCAATA	20	7.026245E-4	45.0	28
ATTGCGA	20	7.026245E-4	45.0	22
GATTCGA	20	7.026245E-4	45.0	41
>>END_MODULE