FastQCFastQC Report
Sat 18 Jun 2016
SRR3551558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413373
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.49059807970041586No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC12390.2997293001719996No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT7910.19135260406460994Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7080.17127388581257122No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC6870.16619372818253733No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC5420.13111644930849378No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC5040.1219237831208134No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4450.10765095930309913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGT351.2094097E-745.00000414
CGAACGG253.8864564E-545.02
ACGTTAG207.028039E-445.01
ATAGCCC253.8864564E-545.013
ACGGGTA207.028039E-445.05
TACGCGG207.028039E-445.02
CACTATC207.028039E-445.036
GACGGTC207.028039E-445.029
ACTAAGG207.028039E-445.02
ATGGTCC253.8864564E-545.011
TGTAGCG406.7975634E-945.01
TTACGAC207.028039E-445.030
CGGTCTA207.028039E-445.031
ATACGAG253.8864564E-545.01
GTACGAG253.8864564E-545.01
CCATAGT302.1621163E-644.99999618
CGAATAT800.042.187514
CGTTAGG556.002665E-1140.9090922
TAAGCAG556.002665E-1140.9090921
TTACACG501.0786607E-940.534