##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413373
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41023482423864	33.0	31.0	34.0	31.0	34.0
2	32.52805093704717	34.0	31.0	34.0	31.0	34.0
3	32.53607032873458	34.0	31.0	34.0	31.0	34.0
4	36.03579817743297	37.0	35.0	37.0	35.0	37.0
5	36.115082504179036	37.0	35.0	37.0	35.0	37.0
6	36.12930694554313	37.0	36.0	37.0	35.0	37.0
7	36.23516291581695	37.0	37.0	37.0	35.0	37.0
8	36.15208056646177	37.0	37.0	37.0	35.0	37.0
9	37.986687567886634	39.0	38.0	39.0	35.0	39.0
10	37.72859378817678	39.0	37.0	39.0	35.0	39.0
11	37.70093353944259	39.0	37.0	39.0	35.0	39.0
12	37.653796934003914	39.0	37.0	39.0	35.0	39.0
13	37.61321857015335	39.0	37.0	39.0	35.0	39.0
14	38.94838317935618	40.0	38.0	41.0	35.0	41.0
15	39.027389306993925	40.0	38.0	41.0	35.0	41.0
16	39.034382990664604	40.0	38.0	41.0	35.0	41.0
17	38.99673418438069	40.0	38.0	41.0	35.0	41.0
18	38.932794352800016	40.0	38.0	41.0	35.0	41.0
19	38.8569863053465	40.0	38.0	41.0	35.0	41.0
20	38.78442713965353	40.0	38.0	41.0	35.0	41.0
21	38.67241934040201	40.0	38.0	41.0	35.0	41.0
22	38.657834449758454	40.0	38.0	41.0	35.0	41.0
23	38.61468455849801	40.0	38.0	41.0	35.0	41.0
24	38.55089713164624	40.0	38.0	41.0	35.0	41.0
25	38.42420767684391	40.0	37.0	41.0	35.0	41.0
26	38.429174135707946	40.0	37.0	41.0	35.0	41.0
27	38.44994230392406	40.0	37.0	41.0	35.0	41.0
28	38.39196560975197	40.0	37.0	41.0	35.0	41.0
29	38.35711330928726	40.0	37.0	41.0	35.0	41.0
30	38.20737203445798	40.0	37.0	41.0	34.0	41.0
31	38.180950376536444	40.0	37.0	41.0	34.0	41.0
32	38.06785397207849	40.0	37.0	41.0	34.0	41.0
33	37.973041296843284	40.0	37.0	41.0	34.0	41.0
34	37.84297716590101	40.0	37.0	41.0	34.0	41.0
35	37.738071910840816	40.0	37.0	41.0	34.0	41.0
36	37.629893582793265	40.0	36.0	41.0	33.0	41.0
37	37.549544842067576	40.0	36.0	41.0	33.0	41.0
38	37.39962939040527	40.0	36.0	41.0	33.0	41.0
39	37.38308017214477	40.0	36.0	41.0	33.0	41.0
40	37.27911595580747	40.0	35.0	41.0	33.0	41.0
41	37.20595442856693	40.0	35.0	41.0	33.0	41.0
42	37.13040280811761	40.0	35.0	41.0	33.0	41.0
43	37.09683264267381	40.0	35.0	41.0	33.0	41.0
44	37.04428445979781	39.0	35.0	41.0	33.0	41.0
45	36.95568651073002	39.0	35.0	41.0	32.0	41.0
46	36.907439527980785	39.0	35.0	41.0	32.0	41.0
47	36.87270092628207	39.0	35.0	41.0	32.0	41.0
48	36.81164226981443	39.0	35.0	41.0	32.0	41.0
49	36.75773212086904	39.0	35.0	41.0	32.0	41.0
50	36.67876711831687	39.0	35.0	41.0	32.0	41.0
51	35.869582677146305	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	10.0
11	11.0
12	11.0
13	12.0
14	17.0
15	29.0
16	34.0
17	41.0
18	95.0
19	138.0
20	203.0
21	355.0
22	542.0
23	793.0
24	1237.0
25	1909.0
26	2783.0
27	3438.0
28	3654.0
29	3882.0
30	4284.0
31	5116.0
32	6809.0
33	9762.0
34	19075.0
35	28372.0
36	26548.0
37	41996.0
38	78873.0
39	173112.0
40	224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.916687833990125	20.057188060178095	24.41306035953001	12.613063746301767
2	33.40784231190717	24.00979260861256	24.576593052763485	18.00577202671679
3	32.773790257225315	23.633377119453858	26.528341231768888	17.06449139155194
4	29.46128557017512	25.67511666219129	25.475780953279482	19.387816814354107
5	25.281525401997712	29.2401777571346	24.63924833020057	20.839048510667123
6	26.702518064798618	34.14954532589211	24.445960427991185	14.701976181318082
7	77.77842287715937	7.060693368942819	10.055083423445653	5.1058003304521575
8	77.93590776369041	6.588480621617763	10.07225919448053	5.403352420211286
9	70.73708248966429	9.129285173439	12.571696748457203	7.561935588439496
10	37.19570460576767	27.332457610922823	19.293471029796333	16.17836675351317
11	29.330411033134723	25.39522416800323	25.540371528861343	19.733993270000703
12	26.717274713152527	22.65484199500209	30.48723549917387	20.14064779267151
13	24.243963684130314	24.350646994361025	31.549714180655243	19.855675140853418
14	20.852837509948642	26.907901580412847	30.014055102776428	22.225205806862082
15	20.01533723779734	25.531662687209856	33.65507664990215	20.797923425090655
16	22.57888154281968	24.185904739787066	31.62446507149717	21.61074864589608
17	23.428719340643923	24.14357009287012	28.281963263202968	24.14574730328299
18	23.37815967661168	24.06760964068771	29.672716892491767	22.881513790208842
19	24.200177563604782	26.05298362495857	27.701857644306717	22.04498116712993
20	25.356518205107736	25.048321975552344	28.183263057819453	21.411896761520467
21	24.588446753900232	25.590205456089294	28.10052906213033	21.720818727880147
22	23.11229809397324	24.701177870833362	27.215372073163945	24.97115196202945
23	22.71120755346866	26.353922486471053	27.94667285962073	22.988197100439557
24	22.823454845865598	24.64771525958396	29.512570971011655	23.01625892353879
25	22.373256114937355	26.419964535661496	27.90893454579762	23.297844803603525
26	21.657195801370673	26.407868922256654	28.656201541948796	23.278733734423874
27	23.066092850766744	25.977506997312354	28.341231768886693	22.61516838303421
28	20.41933072551908	26.01161662711401	29.64562271846492	23.923429928901985
29	21.704852518185756	25.340310083145244	28.785140780844422	24.16969661782458
30	22.456232022894575	27.20980809099772	27.54654996818854	22.78740991791917
31	23.569996105212486	27.30149284060643	25.67971299528513	23.44879805889596
32	23.075527429222518	26.769769675329545	27.40890188764143	22.74580100780651
33	22.668147169747417	27.750723922462278	26.232482527886436	23.348646379903865
34	22.2547191035699	26.102091815382234	27.412772483930976	24.23041659711689
35	23.725303781330663	25.239674579616956	27.80346079690739	23.231560842144987
36	24.292104225481587	28.044405415931855	25.420141131617207	22.243349226969347
37	23.796909812687332	27.72677460792069	26.974185541871385	21.50213003752059
38	22.490099740428136	27.848698391041506	26.817426392144622	22.843775476385733
39	24.63223287442576	25.798975743456875	27.215372073163945	22.353419308953416
40	24.739400009192668	25.31152252324172	28.939238895622115	21.009838571943497
41	22.83167986298089	26.231272966545955	27.271011894826223	23.666035275646934
42	22.79587684730256	25.763898464582834	27.93965740384592	23.500567284268687
43	23.289619786488235	25.008164539048266	28.527988039857465	23.174227634606034
44	22.653874345929705	25.372242502534032	27.9720736477709	24.001809503765365
45	22.702014887280978	25.009857924924944	27.86950284609783	24.41862434169624
46	23.35977434423632	26.06919174692106	27.714920906783945	22.856113002058674
47	22.855387265254382	25.57762601814826	29.05293766162763	22.514049054969725
48	22.229802139955922	24.25944606928851	29.16784598897364	24.342905801781924
49	22.245526437382217	25.060659501225285	29.555631354732892	23.138182706659602
50	21.81274538975695	24.249769578564635	30.053002977940018	23.88448205373839
51	22.279394154915778	24.09349425337407	27.70379294245149	25.923318649258658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	227.0
1	513.5
2	800.0
3	2142.5
4	3485.0
5	2431.0
6	1377.0
7	1337.5
8	1298.0
9	1270.0
10	1242.0
11	1208.0
12	1174.0
13	1148.0
14	1122.0
15	1147.0
16	1172.0
17	1145.5
18	1119.0
19	1098.0
20	1077.0
21	1203.0
22	1329.0
23	1532.5
24	1736.0
25	2224.5
26	3206.0
27	3699.0
28	4662.5
29	5626.0
30	6162.0
31	6698.0
32	7623.5
33	8549.0
34	9413.5
35	10278.0
36	11134.5
37	11991.0
38	13036.5
39	14082.0
40	15463.0
41	16844.0
42	18440.0
43	20036.0
44	22835.5
45	25635.0
46	28881.5
47	32128.0
48	35778.0
49	39428.0
50	37940.0
51	36452.0
52	32830.0
53	29208.0
54	26292.0
55	23376.0
56	22219.0
57	21062.0
58	20126.0
59	19190.0
60	18678.5
61	18167.0
62	16550.5
63	14934.0
64	13461.5
65	11989.0
66	10196.0
67	8403.0
68	7017.5
69	5632.0
70	4753.0
71	3874.0
72	3328.5
73	2783.0
74	2158.0
75	1221.0
76	909.0
77	774.0
78	639.0
79	449.5
80	260.0
81	153.0
82	46.0
83	42.5
84	39.0
85	21.5
86	4.0
87	3.5
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.878235720561015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11262646989256	22.98823305036003
2	8.419309969338427	5.367854956140794
3	3.1651950328078313	3.027025000721907
4	1.875801948860182	2.391890267634105
5	1.4115148039928116	2.2498300822372164
6	1.1737787668347024	2.245079772776764
7	0.9849439556803375	2.1978792913503726
8	0.8994920914437656	2.2939376735859827
9	0.8674686089484187	2.488803191662037
>10	8.964317857071903	49.19569045499463
>50	0.08293941799434752	1.8437392269887134
>100	0.03728469249287183	2.1336048204963456
>500	0.003804560458456309	0.7839712118967926
>1k	0.0015218241833825235	0.7924609991543384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.49059807970041586	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1239	0.2997293001719996	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	791	0.19135260406460994	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	708	0.17127388581257122	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	687	0.16619372818253733	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	542	0.13111644930849378	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	504	0.1219237831208134	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	445	0.10765095930309913	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19667467396274066	0.0
2	0.0	0.0	0.0	0.8754804982425074	0.0
3	0.0	0.0	0.0	1.2013363233689671	0.0
4	0.0	0.0	0.0	1.5944437590263516	0.0
5	0.0	0.0	0.0	2.7362696644434927	0.0
6	0.0	0.0	0.0	3.4820851869860876	0.0
7	0.0	0.0	0.0	4.158471888584886	0.0
8	0.0	0.0	0.0	5.227724113572972	0.0
9	0.0	0.0	0.0	5.703807457187576	0.0
10	0.0	0.0	0.0	6.616058620180805	0.0
11	0.0	0.0	0.0	7.786672085501472	0.0
12	0.0	0.0	0.0	8.636509883325713	0.0
13	0.0	0.0	0.0	9.013893021556802	0.0
14	0.0	0.0	0.0	9.167991136334496	0.0
15	0.0	0.0	0.0	9.483202821664696	0.0
16	0.0	0.0	0.0	10.097901894898795	0.0
17	0.0	0.0	0.0	10.815897506610252	0.0
18	0.0	0.0	0.0	11.585178519158243	0.0
19	0.0	0.0	0.0	12.01191176008109	0.0
20	0.0	0.0	0.0	12.44687001811923	0.0
21	0.0	0.0	0.0	12.982221867417563	0.0
22	0.0	0.0	0.0	13.512251646817765	0.0
23	0.0	0.0	0.0	14.046877759311808	0.0
24	0.0	0.0	0.0	14.479658806937076	0.0
25	0.0	0.0	0.0	14.885345680535497	0.0
26	2.41912268096852E-4	0.0	0.0	15.260309696085617	0.0
27	2.41912268096852E-4	0.0	0.0	15.596809661008338	0.0
28	2.41912268096852E-4	0.0	0.0	15.986046500376174	0.0
29	2.41912268096852E-4	0.0	0.0	16.36609067355633	0.0
30	2.41912268096852E-4	0.0	0.0	16.817498965825052	0.0
31	2.41912268096852E-4	0.0	0.0	17.199478437149985	0.0
32	2.41912268096852E-4	0.0	0.0	17.595730732292626	0.0
33	2.41912268096852E-4	0.0	0.0	17.960050608046487	0.0
34	2.41912268096852E-4	0.0	0.0	18.335014623596606	0.0
35	2.41912268096852E-4	0.0	0.0	18.74674930389745	0.0
36	2.41912268096852E-4	0.0	0.0	19.117600810889922	0.0
37	2.41912268096852E-4	0.0	0.0	19.51748179005402	0.0
38	2.41912268096852E-4	0.0	0.0	19.87768915725023	0.0
39	2.41912268096852E-4	0.0	0.0	20.238622261250736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	35	1.2094097E-7	45.000004	14
CGAACGG	25	3.8864564E-5	45.0	2
ACGTTAG	20	7.028039E-4	45.0	1
ATAGCCC	25	3.8864564E-5	45.0	13
ACGGGTA	20	7.028039E-4	45.0	5
TACGCGG	20	7.028039E-4	45.0	2
CACTATC	20	7.028039E-4	45.0	36
GACGGTC	20	7.028039E-4	45.0	29
ACTAAGG	20	7.028039E-4	45.0	2
ATGGTCC	25	3.8864564E-5	45.0	11
TGTAGCG	40	6.7975634E-9	45.0	1
TTACGAC	20	7.028039E-4	45.0	30
CGGTCTA	20	7.028039E-4	45.0	31
ATACGAG	25	3.8864564E-5	45.0	1
GTACGAG	25	3.8864564E-5	45.0	1
CCATAGT	30	2.1621163E-6	44.999996	18
CGAATAT	80	0.0	42.1875	14
CGTTAGG	55	6.002665E-11	40.909092	2
TAAGCAG	55	6.002665E-11	40.909092	1
TTACACG	50	1.0786607E-9	40.5	34
>>END_MODULE