FastQCFastQC Report
Sat 18 Jun 2016
SRR3551555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401867
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG45981.1441596349040852No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC45551.133459577422381No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC43291.0772220660069127No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35690.8881047709814441No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC16810.41829759597080624TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT11560.2876573592755812No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT10770.26799911413477595No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT9900.24635016062528153No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT9390.2336593947748882No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA7550.1878731022950379No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC6450.16050086222556217No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT6360.1582613153107869TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC5200.12939604396479432TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC4640.11546108538397033No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4230.10525870499443846No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4190.10426335081009387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGA351.2092823E-745.00000437
CAACGGC207.027804E-445.00000433
GACAATC207.027804E-445.0000049
TTACTAG207.027804E-445.0000041
CGATGCA207.027804E-445.00000410
GCCCGGT207.027804E-445.00000431
TTTAGCG253.886263E-545.01
CTATCGG253.886263E-545.01
CGAATGC700.041.78571745
CTCGAAT650.041.5384643
CGAGGGA2450.041.326534
CGTTCAT556.002665E-1140.90909217
CCGATGA6350.040.393718
TCGATCA950.040.26315717
GCCTCGA950.040.26315714
CTCGATC950.040.26315716
CGTTTTT15400.040.178571
GCGTTCA900.040.00000437
CGATGAA6800.039.70588319
GATGAAT7200.039.37500420