##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401867
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27115687528461	33.0	31.0	34.0	30.0	34.0
2	32.388193108665305	34.0	31.0	34.0	30.0	34.0
3	32.39963968178527	34.0	31.0	34.0	30.0	34.0
4	35.92185723137257	37.0	35.0	37.0	35.0	37.0
5	35.98083196679499	37.0	35.0	37.0	35.0	37.0
6	35.99861148091284	37.0	35.0	37.0	35.0	37.0
7	36.10866779307582	37.0	36.0	37.0	35.0	37.0
8	35.95862810332772	37.0	35.0	37.0	35.0	37.0
9	37.80048871890451	39.0	38.0	39.0	35.0	39.0
10	37.58331487780783	39.0	37.0	39.0	35.0	39.0
11	37.5474074756076	39.0	37.0	39.0	35.0	39.0
12	37.50522187688962	39.0	37.0	39.0	35.0	39.0
13	37.4533489935725	39.0	37.0	39.0	35.0	39.0
14	38.81188552431526	40.0	38.0	41.0	35.0	41.0
15	38.79367800789814	40.0	38.0	41.0	35.0	41.0
16	38.88572587448086	40.0	38.0	41.0	35.0	41.0
17	38.757900001741866	40.0	38.0	41.0	35.0	41.0
18	38.70016945904988	40.0	38.0	41.0	35.0	41.0
19	38.618134855561664	40.0	38.0	41.0	35.0	41.0
20	38.39243829426153	40.0	37.0	41.0	34.0	41.0
21	38.37623392814041	40.0	37.0	41.0	34.0	41.0
22	38.363154974158114	40.0	37.0	41.0	34.0	41.0
23	38.2480970072188	40.0	37.0	41.0	34.0	41.0
24	38.27358553949441	40.0	37.0	41.0	34.0	41.0
25	38.21075878337858	40.0	37.0	41.0	34.0	41.0
26	38.161314564271265	40.0	37.0	41.0	34.0	41.0
27	38.09163977136714	40.0	37.0	41.0	34.0	41.0
28	37.99152455912031	40.0	37.0	41.0	34.0	41.0
29	37.92694349125457	40.0	37.0	41.0	34.0	41.0
30	37.79817700881137	40.0	36.0	41.0	33.0	41.0
31	37.743586808570996	40.0	36.0	41.0	33.0	41.0
32	37.52045577268101	40.0	36.0	41.0	33.0	41.0
33	37.36515314768319	40.0	36.0	41.0	33.0	41.0
34	37.16394478770339	40.0	36.0	41.0	32.0	41.0
35	37.074686401222294	40.0	36.0	41.0	31.0	41.0
36	36.96236565828993	40.0	35.0	41.0	31.0	41.0
37	36.86921046017712	40.0	35.0	41.0	31.0	41.0
38	36.7060943048322	40.0	35.0	41.0	30.0	41.0
39	36.71251682770668	40.0	35.0	41.0	31.0	41.0
40	36.63982113485307	40.0	35.0	41.0	30.0	41.0
41	36.57271684413002	40.0	35.0	41.0	30.0	41.0
42	36.45620317169611	40.0	35.0	41.0	30.0	41.0
43	36.466758902821084	39.0	35.0	41.0	30.0	41.0
44	36.50063080571433	39.0	35.0	41.0	30.0	41.0
45	36.38955176712693	39.0	35.0	41.0	30.0	41.0
46	36.3346156813075	39.0	35.0	41.0	30.0	41.0
47	36.22170519102091	39.0	35.0	41.0	30.0	41.0
48	36.15230909728841	39.0	35.0	41.0	30.0	41.0
49	36.111554320210416	39.0	35.0	41.0	29.0	41.0
50	36.02052918005211	39.0	35.0	41.0	29.0	41.0
51	35.27825375061899	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	10.0
11	7.0
12	7.0
13	16.0
14	10.0
15	20.0
16	29.0
17	53.0
18	111.0
19	264.0
20	579.0
21	1057.0
22	1547.0
23	2010.0
24	2478.0
25	3160.0
26	4026.0
27	4537.0
28	4637.0
29	4697.0
30	5167.0
31	6195.0
32	7607.0
33	10092.0
34	17848.0
35	26161.0
36	25648.0
37	37609.0
38	71413.0
39	164619.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.146030900770654	18.59520687192529	22.96705128811273	13.291710939191326
2	30.804221297095808	29.84071844665026	23.566254507088168	15.788805749165768
3	29.419683626672505	28.905583190458533	26.650857124371996	15.023876058496965
4	25.988448914690682	23.297011200222958	32.9735957418748	17.74094414321156
5	28.723433374723477	27.185611159911115	24.5640970768936	19.52685838847181
6	24.842298571417906	37.31632604817017	23.97111482157032	13.870260558841608
7	74.2703431732389	5.51276914999241	15.896801678167154	4.320085998601527
8	74.4679209788313	10.417874570442459	10.513677410685625	4.60052704004061
9	67.44246230718123	8.43786625923502	12.17591889854106	11.94375253504269
10	36.47425640821466	30.367509648714623	18.911480664000777	14.24675327906994
11	29.097188870944862	23.347774263624533	28.268307673931925	19.28672919149868
12	24.19556719014002	20.90666812651947	31.740351907471876	23.157412775868632
13	24.68851634993667	22.494755727641234	34.51465285778628	18.30207506463581
14	20.242766885561643	28.579107017993515	29.664043078929097	21.514083017515745
15	18.9254156225816	24.116685371030712	34.968285527301326	21.989613479086366
16	21.024368758818216	24.525029425158074	29.483137455924474	24.967464360099235
17	20.756120806137353	25.74359178534192	30.31749310095131	23.18279430756942
18	21.553648346343444	23.288799528202116	30.596441111113876	24.561111014340565
19	21.558127440172992	25.04286243956334	28.838396783015273	24.560613337248395
20	23.896463257744475	24.422507944170587	31.45368990238064	20.2273388957043
21	22.60225397955045	28.06724612869432	29.63094755229964	19.699552339455593
22	21.09503890590668	22.66670316298676	31.684363234602493	24.553894696504067
23	21.81194275718086	26.095200650961637	30.10772220660069	21.985134385256814
24	21.170934662462955	25.558207068507738	29.618007947903163	23.65285032112614
25	20.568994219480572	30.021126392562714	27.459831237698047	21.950048150258667
26	20.238038953186006	24.938599088753243	30.726085993624757	24.097275964435998
27	24.266983852866744	24.964975974638374	28.227498152373798	22.540542020121084
28	20.070819450216113	26.351753191976453	31.588062717267157	21.98936464054028
29	23.84495367870465	23.40973506159998	29.55156805609816	23.19374320359721
30	23.10913809792792	26.975093749922234	29.384846230220447	20.530921921929394
31	24.06791301599783	25.25586823501308	27.236871900404857	23.439346848584233
32	24.54020857646933	28.87646908056646	26.335329847934762	20.24799249502945
33	22.88418805226605	26.78075084542896	26.141733459079745	24.193327643225246
34	24.590971639870904	25.694819430309035	27.171178524238123	22.543030405581945
35	24.556383081964928	26.908902696663322	26.119089151385904	22.415625069985843
36	23.874067788596726	27.62978796467488	26.90765850393289	21.588485742795502
37	22.472111419947396	28.001801591073665	28.11353009826634	21.4125568907126
38	19.720454777326825	28.509929902181568	27.56160620304728	24.20800911744433
39	23.94797283678431	27.205767082144096	26.504788897819427	22.34147118325217
40	22.188186638863105	27.23214396802922	28.611456028984716	21.968213364122956
41	21.790044965125276	25.656498294211765	26.818574304434055	25.7348824362289
42	21.693495609243854	26.54883332047667	29.37091127163962	22.38675979863985
43	23.088982175694944	24.791286669470246	27.521294358581322	24.598436796253488
44	22.935448792759793	24.529508518987626	28.932706591981926	23.60233609627066
45	21.9172014621753	24.532245742994576	28.23944240258593	25.311110392244203
46	24.181632231559195	26.966882077901396	26.879042071132986	21.97244361940642
47	20.702371680182747	25.736375467505418	31.8782084620036	21.683044390308236
48	21.661893113890915	25.377301445503115	28.861538767801285	24.099266672804685
49	21.238618746998387	24.499647893457286	31.532074044397774	22.729659315146552
50	21.934371321855238	23.5189751833318	30.639987856678953	23.906665638134008
51	22.05953711053657	23.205189776717173	28.334996404283007	26.40027670846325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	447.0
2	745.0
3	3165.5
4	5586.0
5	3694.5
6	1803.0
7	1732.0
8	1661.0
9	1564.5
10	1468.0
11	1425.5
12	1383.0
13	1362.0
14	1341.0
15	1314.5
16	1288.0
17	1180.5
18	1073.0
19	1082.5
20	1092.0
21	1238.0
22	1384.0
23	1514.5
24	1645.0
25	2141.0
26	3262.0
27	3887.0
28	4554.0
29	5221.0
30	5830.5
31	6440.0
32	7008.5
33	7577.0
34	8923.5
35	10270.0
36	10831.0
37	11392.0
38	12236.0
39	13080.0
40	14989.0
41	16898.0
42	18121.5
43	19345.0
44	21399.0
45	23453.0
46	35332.0
47	47211.0
48	42566.5
49	37922.0
50	36752.0
51	35582.0
52	31412.0
53	27242.0
54	25110.0
55	22978.0
56	21174.0
57	19370.0
58	18195.5
59	17021.0
60	15887.5
61	14754.0
62	13208.5
63	11663.0
64	10590.0
65	9517.0
66	7923.5
67	6330.0
68	5379.5
69	4429.0
70	3626.5
71	2824.0
72	2330.5
73	1837.0
74	1485.0
75	860.0
76	587.0
77	472.5
78	358.0
79	261.5
80	165.0
81	127.0
82	89.0
83	58.5
84	28.0
85	17.0
86	6.0
87	4.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.330117622657355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54588183119736	24.905086563044065
2	7.763320893229977	5.33031438814037
3	3.310750402771176	3.4097535223918403
4	2.2139191341228317	3.040164171259541
5	1.691413896101263	2.903321900087675
6	1.4875183311794271	3.0640007565149228
7	1.348017645884081	3.2394323038432717
8	1.2412374215860966	3.4089461344555705
9	1.1254847147121196	3.477422037471302
>10	7.164931700012228	37.058227418231624
>50	0.06611274768591975	1.573546689390362
>100	0.03196660327670845	2.242222973152537
>500	0.004359082265005697	1.1186173564615272
>1k	0.00508559597583998	5.228943785555388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	4598	1.1441596349040852	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	4555	1.133459577422381	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	4329	1.0772220660069127	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3569	0.8881047709814441	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1681	0.41829759597080624	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	1156	0.2876573592755812	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	1077	0.26799911413477595	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	990	0.24635016062528153	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	939	0.2336593947748882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	755	0.1878731022950379	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	645	0.16050086222556217	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	636	0.1582613153107869	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	520	0.12939604396479432	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	464	0.11546108538397033	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	423	0.10525870499443846	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	419	0.10426335081009387	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16945904988466334	0.0
2	0.0	0.0	0.0	0.8726767811241033	0.0
3	0.0	0.0	0.0	1.157845754938823	0.0
4	0.0	0.0	0.0	1.7020556552292176	0.0
5	0.0	0.0	0.0	3.6915198311879305	0.0
6	0.0	0.0	0.0	4.571910607240704	0.0
7	0.0	0.0	0.0	5.361724152518121	0.0
8	0.0	0.0	0.0	6.368276071436569	0.0
9	0.0	0.0	0.0	6.745266468757077	0.0
10	0.0	0.0	0.0	8.62822774699092	0.0
11	0.0	0.0	0.0	9.606660910201635	0.0
12	0.0	0.0	0.0	11.53068054853969	0.0
13	0.0	0.0	0.0	11.969134066743475	0.0
14	0.0	0.0	0.0	12.171190966165423	0.0
15	0.0	0.0	0.0	12.629551568056097	0.0
16	0.0	0.0	0.0	13.144398519908329	0.0
17	0.0	0.0	0.0	13.76425533820891	0.0
18	0.0	0.0	0.0	14.406756464203331	0.0
19	0.0	0.0	0.0	15.100518330691498	0.0
20	0.0	0.0	0.0	15.529018307051835	0.0
21	0.0	0.0	0.0	15.958513637596518	0.0
22	2.488385460861429E-4	0.0	0.0	16.423344041685432	0.0
23	2.488385460861429E-4	0.0	0.0	16.871999940278748	0.0
24	2.488385460861429E-4	0.0	0.0	17.24351588958536	0.0
25	2.488385460861429E-4	0.0	0.0	17.576213025702533	0.0
26	2.488385460861429E-4	0.0	0.0	17.945987105186543	0.0
27	2.488385460861429E-4	0.0	0.0	18.31899608576967	0.0
28	2.488385460861429E-4	0.0	0.0	18.65443044589379	0.0
29	2.488385460861429E-4	0.0	0.0	18.99782763949267	0.0
30	2.488385460861429E-4	0.0	0.0	19.393729766315722	0.0
31	2.488385460861429E-4	0.0	0.0	19.72592922534072	0.0
32	2.488385460861429E-4	0.0	0.0	20.069077580393515	0.0
33	2.488385460861429E-4	0.0	0.0	20.41570967509151	0.0
34	4.976770921722858E-4	0.0	0.0	20.753383582130407	0.0
35	4.976770921722858E-4	0.0	0.0	21.09752729136754	0.0
36	4.976770921722858E-4	0.0	0.0	21.43246397439949	0.0
37	4.976770921722858E-4	0.0	0.0	21.761926209417542	0.0
38	4.976770921722858E-4	0.0	0.0	22.082679095322582	0.0
39	4.976770921722858E-4	0.0	0.0	22.437274023495334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGGA	35	1.2092823E-7	45.000004	37
CAACGGC	20	7.027804E-4	45.000004	33
GACAATC	20	7.027804E-4	45.000004	9
TTACTAG	20	7.027804E-4	45.000004	1
CGATGCA	20	7.027804E-4	45.000004	10
GCCCGGT	20	7.027804E-4	45.000004	31
TTTAGCG	25	3.886263E-5	45.0	1
CTATCGG	25	3.886263E-5	45.0	1
CGAATGC	70	0.0	41.785717	45
CTCGAAT	65	0.0	41.53846	43
CGAGGGA	245	0.0	41.32653	4
CGTTCAT	55	6.002665E-11	40.909092	17
CCGATGA	635	0.0	40.3937	18
TCGATCA	95	0.0	40.263157	17
GCCTCGA	95	0.0	40.263157	14
CTCGATC	95	0.0	40.263157	16
CGTTTTT	1540	0.0	40.17857	1
GCGTTCA	90	0.0	40.000004	37
CGATGAA	680	0.0	39.705883	19
GATGAAT	720	0.0	39.375004	20
>>END_MODULE