FastQCFastQC Report
Sat 18 Jun 2016
SRR3551554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492114
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56791.154000902229971No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC9700.19710879999349745TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT5990.12171976411969586TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC5960.12111014927435512No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5680.11542041071784181No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC5010.10180567917189919TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG406.8012014E-945.02
TAACGAT207.0293504E-445.013
GTATGCG207.0293504E-445.01
CGAATAT850.045.014
TTAACGA207.0293504E-445.012
CTCGCAA207.0293504E-445.014
TCGAGTG207.0293504E-445.01
GCTACGA850.045.010
CGTTTTT23250.041.6129041
ATAGCGG603.6379788E-1241.2499962
GCGCGAC556.002665E-1140.9090929
AGCTACG950.040.2631579
TATTGCG451.9244908E-840.01
CGGGTAC403.452842E-739.3756
TACGAAT1000.038.2512
AGACCTA950.037.89473732
CGCGCAA301.1389734E-437.49999618
TGTTGCG301.1389734E-437.4999961
CATACGA601.546141E-1037.49999618
TATACGG301.1389734E-437.4999962