##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	492114
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37313508658563	33.0	31.0	34.0	31.0	34.0
2	32.50201172898962	34.0	31.0	34.0	31.0	34.0
3	32.4906180275302	34.0	31.0	34.0	31.0	34.0
4	36.009442933954325	37.0	35.0	37.0	35.0	37.0
5	36.086975782034244	37.0	35.0	37.0	35.0	37.0
6	36.09242167465262	37.0	36.0	37.0	35.0	37.0
7	36.220855330269	37.0	37.0	37.0	35.0	37.0
8	36.13766728847381	37.0	37.0	37.0	35.0	37.0
9	37.96278707779092	39.0	38.0	39.0	35.0	39.0
10	37.6770504395323	39.0	37.0	39.0	35.0	39.0
11	37.63608228987592	39.0	37.0	39.0	35.0	39.0
12	37.59099314386504	39.0	37.0	39.0	35.0	39.0
13	37.586581564434255	39.0	37.0	39.0	35.0	39.0
14	38.92847592224566	40.0	38.0	41.0	35.0	41.0
15	38.977480827613114	40.0	38.0	41.0	35.0	41.0
16	38.98948617596736	40.0	38.0	41.0	35.0	41.0
17	38.942598666162716	40.0	38.0	41.0	35.0	41.0
18	38.83312606428592	40.0	38.0	41.0	35.0	41.0
19	38.71334893947338	40.0	38.0	41.0	35.0	41.0
20	38.57995301901592	40.0	38.0	41.0	35.0	41.0
21	38.47777141068939	40.0	37.0	41.0	35.0	41.0
22	38.456902262483894	40.0	37.0	41.0	35.0	41.0
23	38.436532998451575	40.0	37.0	41.0	35.0	41.0
24	38.35930089369536	40.0	37.0	41.0	35.0	41.0
25	38.24007851839208	40.0	37.0	41.0	34.0	41.0
26	38.24233612536933	40.0	37.0	41.0	34.0	41.0
27	38.21713261561346	40.0	37.0	41.0	34.0	41.0
28	38.1405731192366	40.0	36.0	41.0	34.0	41.0
29	38.09600011379477	40.0	36.0	41.0	34.0	41.0
30	37.917330130823345	40.0	36.0	41.0	34.0	41.0
31	37.84682207781124	40.0	36.0	41.0	34.0	41.0
32	37.68976090905766	40.0	36.0	41.0	33.0	41.0
33	37.503934047801934	40.0	36.0	41.0	33.0	41.0
34	37.307207273111516	40.0	36.0	41.0	33.0	41.0
35	37.19774279943265	40.0	35.0	41.0	32.0	41.0
36	37.094033089893806	40.0	35.0	41.0	32.0	41.0
37	37.023360440873454	40.0	35.0	41.0	32.0	41.0
38	36.868802757084744	40.0	35.0	41.0	31.0	41.0
39	36.824648353836714	40.0	35.0	41.0	31.0	41.0
40	36.73444567722113	40.0	35.0	41.0	31.0	41.0
41	36.633682439434764	39.0	35.0	41.0	31.0	41.0
42	36.563219091511314	39.0	35.0	41.0	31.0	41.0
43	36.537531953978146	39.0	35.0	41.0	31.0	41.0
44	36.47193130047103	39.0	35.0	41.0	31.0	41.0
45	36.36108300109324	39.0	35.0	41.0	31.0	41.0
46	36.27518217323628	39.0	35.0	41.0	30.0	41.0
47	36.20772422650036	39.0	35.0	41.0	30.0	41.0
48	36.12170350772382	39.0	35.0	41.0	30.0	41.0
49	36.020200603925105	39.0	35.0	41.0	30.0	41.0
50	35.911937071491565	38.0	35.0	40.0	30.0	41.0
51	35.06795173476065	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	11.0
11	8.0
12	9.0
13	14.0
14	23.0
15	22.0
16	32.0
17	78.0
18	116.0
19	193.0
20	339.0
21	496.0
22	820.0
23	1169.0
24	1885.0
25	3140.0
26	4781.0
27	6007.0
28	6210.0
29	6021.0
30	6380.0
31	7169.0
32	8952.0
33	12314.0
34	23781.0
35	33916.0
36	33464.0
37	50173.0
38	93223.0
39	191132.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43897552193191	19.79581966780055	23.74774950519595	15.01745530507159
2	35.3672116623384	23.483379867266528	24.28400736414733	16.86540110624774
3	31.884888460803793	22.434435923383607	28.898791743376535	16.78188387243606
4	28.404597308753665	24.99786634804131	28.089223228764066	18.508313114440963
5	25.173232218550982	28.591342656376366	26.46805415005466	19.767370975017982
6	26.344099131502052	33.49000434858589	25.774718865953822	14.391177653958229
7	76.67755845190342	6.43143661834453	11.976290046615215	4.914714883136835
8	77.17378493601076	6.081314492170513	11.653194178584638	5.091706393234088
9	70.2950942261346	8.356600299930504	14.359274477052065	6.989030996882836
10	37.34886631959262	25.55038060286844	21.572643737020282	15.528109340518661
11	28.949999390385155	23.401285068093976	27.897397757430188	19.751317784090677
12	25.16164953648951	21.96686133700728	32.55505838078169	20.31643074572152
13	23.828828279626265	22.091425970405233	33.962862263621844	20.11688348634666
14	21.664085963821393	24.59003401650837	32.23541699687471	21.51046302279553
15	20.8197694030245	23.739011692412735	34.80453716008892	20.636681744473844
16	23.230186501501684	23.414696594691474	32.59265129624437	20.762465607562476
17	23.49902664829694	23.276110819850686	30.243602092198152	22.98126043965423
18	23.748155915092845	23.372226760466074	30.8213137606327	22.058303563808384
19	24.351471407031706	24.845462636706127	29.481786740470707	21.321279215791463
20	24.530901376510318	24.49290205115075	29.980248479010964	20.995948093327968
21	24.611370536095293	24.711347370731172	30.17065151570571	20.50663057746782
22	22.784354844609176	23.139760299442813	29.92599275777564	24.149892098172373
23	22.625042165026805	24.760929378152216	29.914816485611055	22.699211971209923
24	22.54985633410145	23.840207756739293	30.735358067439662	22.8745778417196
25	23.22652881242964	24.37219831177329	29.418386796555268	22.9828860792418
26	22.389934039673733	25.098249592574078	29.244646565633165	23.26716980211902
27	23.241362773666264	24.609541691559272	30.104609907460468	22.044485627313996
28	22.425494905651945	24.875130559179375	29.679098745412645	23.02027578975603
29	24.039958221062598	24.58170261362205	28.675469505033384	22.70286966028197
30	23.551859934893134	25.08178999174988	28.309700597828957	23.05664947552803
31	23.667280345610976	26.17340697480665	26.799684625920012	23.359628053662362
32	24.170822207862404	26.403434976448548	27.149197137248688	22.276545678440364
33	23.282410173252536	26.62167709108052	26.68080973107857	23.415103004588367
34	22.424682085858155	25.752772731521556	28.13555395700996	23.686991225610328
35	23.38807674644493	25.702174699358277	27.689315890220556	23.220432663976233
36	22.947122008315148	27.83155935413339	26.156947373982455	23.06437126356901
37	23.038970645013148	27.411941135590535	27.411128315796745	22.137959903599572
38	22.209284840504438	28.20464363948191	26.43797981768452	23.148091702329136
39	23.348451781497783	25.393709587615877	27.822008721556386	23.435829909329954
40	22.85100606769976	25.25105971380615	29.593549462116503	22.304384756377587
41	20.520854923859105	26.94152167993595	28.182900709998087	24.354722686206852
42	21.72301539887099	26.645655274997253	27.888050329801633	23.74327899633012
43	23.211288441296123	24.792426145161485	27.83846832238059	24.157817091161803
44	23.0517725567653	24.51119049651097	28.285316004015332	24.151720942708398
45	23.293789650365564	23.937746131993805	28.23024746298622	24.53821675465441
46	22.51104418894809	26.022222493162158	28.170911618039725	23.295821699850034
47	22.14994899555794	25.40712111421337	29.47995789593468	22.962971994294005
48	21.436496421560857	24.93548242886811	29.25948052686979	24.368540622701243
49	22.086549051642507	24.743860162482676	29.51247068768619	23.65712009818863
50	21.421662460324235	24.446571322904855	30.026985617153752	24.10478059961716
51	21.55008798774268	24.349236152598788	28.942277602344173	25.158398257314364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	573.5
2	956.0
3	4970.5
4	8985.0
5	5767.0
6	2549.0
7	2448.5
8	2348.0
9	2320.5
10	2293.0
11	2194.0
12	2095.0
13	2040.0
14	1985.0
15	1927.5
16	1870.0
17	1854.0
18	1838.0
19	1834.0
20	1830.0
21	1856.0
22	1882.0
23	1957.5
24	2033.0
25	2531.0
26	3662.0
27	4295.0
28	4806.0
29	5317.0
30	6349.0
31	7381.0
32	8305.0
33	9229.0
34	9770.5
35	10312.0
36	11299.5
37	12287.0
38	13921.5
39	15556.0
40	17029.5
41	18503.0
42	20619.5
43	22736.0
44	24656.0
45	26576.0
46	31151.5
47	35727.0
48	40098.5
49	44470.0
50	43789.5
51	43109.0
52	38976.0
53	34843.0
54	31905.5
55	28968.0
56	27781.5
57	26595.0
58	25655.5
59	24716.0
60	23400.5
61	22085.0
62	20568.0
63	19051.0
64	17295.5
65	15540.0
66	13258.0
67	10976.0
68	9305.5
69	7635.0
70	6318.0
71	5001.0
72	4037.5
73	3074.0
74	2489.0
75	1560.0
76	1216.0
77	947.0
78	678.0
79	494.5
80	311.0
81	190.5
82	70.0
83	55.0
84	40.0
85	29.0
86	18.0
87	11.5
88	5.0
89	3.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	492114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.12498522729368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43542723465444	24.377328990879953
2	7.7855395743044635	5.313628459193003
3	3.295607885305713	3.3738771120303013
4	2.2781657778102886	3.109694940524084
5	1.7826844009710914	3.041703942403269
6	1.5242786234020027	3.1209591303523734
7	1.376964788157025	3.289223213805344
8	1.2063339586285053	3.293290281390436
9	1.1647469989076331	3.5772276718131866
>10	8.054514265969436	42.06186714597367
>50	0.057441895133674405	1.32783390209241
>100	0.03470447830992827	2.2934384727513373
>500	0.0029917653715455404	0.6603436628274416
>1k	0.0	0.0
>5k	5.983530743091081E-4	1.159583073963216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5679	1.154000902229971	No Hit
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	970	0.19710879999349745	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	599	0.12171976411969586	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	596	0.12111014927435512	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	568	0.11542041071784181	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	501	0.10180567917189919	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12598706803708085	0.0
2	0.0	0.0	0.0	0.5594232230743283	0.0
3	0.0	0.0	0.0	0.8278569599726893	0.0
4	0.0	0.0	0.0	1.1702572981057235	0.0
5	0.0	0.0	0.0	2.114753898486936	0.0
6	0.0	0.0	0.0	2.933873045676408	0.0
7	0.0	0.0	0.0	3.6268019198803527	0.0
8	0.0	0.0	0.0	4.697488793247093	0.0
9	0.0	0.0	0.0	5.251831892610249	0.0
10	0.0	0.0	0.0	6.141869566807691	0.0
11	0.0	0.0	0.0	7.149359701207444	0.0
12	0.0	0.0	0.0	7.964617954376425	0.0
13	0.0	0.0	0.0	8.307424702406353	0.0
14	0.0	0.0	0.0	8.45921879889619	0.0
15	0.0	0.0	0.0	8.721962797238039	0.0
16	0.0	0.0	0.0	9.210467493304398	0.0
17	0.0	0.0	0.0	9.765013797616	0.0
18	0.0	0.0	0.0	10.368532494503306	0.0
19	0.0	0.0	0.0	10.735520631398416	0.0
20	0.0	0.0	0.0	11.108401711798486	0.0
21	0.0	0.0	0.0	11.522939806630172	0.0
22	0.0	0.0	0.0	11.961456085378591	0.0
23	0.0	0.0	0.0	12.400175569075458	0.0
24	0.0	0.0	0.0	12.781794462258745	0.0
25	0.0	0.0	0.0	13.120333906371288	0.0
26	0.0	0.0	0.0	13.4314406824435	0.0
27	0.0	0.0	0.0	13.747221172329988	0.0
28	0.0	0.0	0.0	14.079054853143784	0.0
29	0.0	0.0	0.0	14.408653279524662	0.0
30	0.0	0.0	0.0	14.786411278687458	0.0
31	2.0320494844690458E-4	0.0	0.0	15.136126994964581	0.0
32	2.0320494844690458E-4	0.0	0.0	15.476698488561594	0.0
33	2.0320494844690458E-4	0.0	0.0	15.812189858447432	0.0
34	2.0320494844690458E-4	0.0	0.0	16.136708161117138	0.0
35	2.0320494844690458E-4	0.0	0.0	16.479311704198622	0.0
36	2.0320494844690458E-4	0.0	0.0	16.80159475243541	0.0
37	2.0320494844690458E-4	0.0	0.0	17.134444457991442	0.0
38	2.0320494844690458E-4	0.0	0.0	17.470139032825728	0.0
39	2.0320494844690458E-4	0.0	0.0	17.822699618381108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	40	6.8012014E-9	45.0	2
TAACGAT	20	7.0293504E-4	45.0	13
GTATGCG	20	7.0293504E-4	45.0	1
CGAATAT	85	0.0	45.0	14
TTAACGA	20	7.0293504E-4	45.0	12
CTCGCAA	20	7.0293504E-4	45.0	14
TCGAGTG	20	7.0293504E-4	45.0	1
GCTACGA	85	0.0	45.0	10
CGTTTTT	2325	0.0	41.612904	1
ATAGCGG	60	3.6379788E-12	41.249996	2
GCGCGAC	55	6.002665E-11	40.909092	9
AGCTACG	95	0.0	40.263157	9
TATTGCG	45	1.9244908E-8	40.0	1
CGGGTAC	40	3.452842E-7	39.375	6
TACGAAT	100	0.0	38.25	12
AGACCTA	95	0.0	37.894737	32
CGCGCAA	30	1.1389734E-4	37.499996	18
TGTTGCG	30	1.1389734E-4	37.499996	1
CATACGA	60	1.546141E-10	37.499996	18
TATACGG	30	1.1389734E-4	37.499996	2
>>END_MODULE