FastQCFastQC Report
Sat 18 Jun 2016
SRR3551553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400905
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49401.232212120078323No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC7510.18732617453012557No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT6200.15465010413938465No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC6050.15090856936181887No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5250.1309537172148015No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC5010.12496726157069629No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4060.10127087464611316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC302.1619526E-645.00000412
TGATGCG302.1619526E-645.0000041
GTAACGG600.045.0000042
TCGTTTA253.8862454E-545.038
CGCATCG253.8862454E-545.021
TGCATAG351.2092823E-745.01
CCGTACT207.027785E-445.029
TGCGACG207.027785E-445.01
CAATGCG253.8862454E-545.01
CGGTAAT253.8862454E-545.06
TATCGAG207.027785E-445.01
GTGATAG556.002665E-1140.9090921
CGTTTTT19650.040.763361
ACTGCGG403.4507138E-739.3752
GCGTAAG403.4507138E-739.3751
AATGCGG750.039.0000042
TCGGGAT700.038.5714265
CTACGAA356.238117E-638.57142611
TATTAGT356.238117E-638.57142630
CGTAAGG700.038.5714262