##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400905
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.455481972038264	33.0	31.0	34.0	31.0	34.0
2	32.57419588181739	34.0	31.0	34.0	31.0	34.0
3	32.57032214614435	34.0	31.0	34.0	31.0	34.0
4	36.06217682493359	37.0	35.0	37.0	35.0	37.0
5	36.14466519499632	37.0	35.0	37.0	35.0	37.0
6	36.16968109651913	37.0	37.0	37.0	35.0	37.0
7	36.2833389456355	37.0	37.0	37.0	35.0	37.0
8	36.21922899440017	37.0	37.0	37.0	35.0	37.0
9	38.05444930843966	39.0	39.0	39.0	37.0	39.0
10	37.77238996769808	39.0	38.0	39.0	35.0	39.0
11	37.72969406717302	39.0	37.0	39.0	35.0	39.0
12	37.69076464499071	39.0	37.0	39.0	35.0	39.0
13	37.69135081877253	39.0	37.0	39.0	35.0	39.0
14	39.04162582157868	40.0	39.0	41.0	35.0	41.0
15	39.09033312131303	40.0	39.0	41.0	35.0	41.0
16	39.092398448510245	40.0	39.0	41.0	35.0	41.0
17	39.03758246966239	40.0	38.0	41.0	35.0	41.0
18	38.932435364986716	40.0	38.0	41.0	35.0	41.0
19	38.82254399421309	40.0	38.0	41.0	35.0	41.0
20	38.7101183572168	40.0	38.0	41.0	35.0	41.0
21	38.60617852109602	40.0	38.0	41.0	35.0	41.0
22	38.58605904141879	40.0	38.0	41.0	35.0	41.0
23	38.555263715842905	40.0	37.0	41.0	35.0	41.0
24	38.47783639515596	40.0	37.0	41.0	35.0	41.0
25	38.372357541063344	40.0	37.0	41.0	35.0	41.0
26	38.38598171636672	40.0	37.0	41.0	35.0	41.0
27	38.38656789014854	40.0	37.0	41.0	35.0	41.0
28	38.304513538120005	40.0	37.0	41.0	35.0	41.0
29	38.24229680348212	40.0	37.0	41.0	35.0	41.0
30	38.09996882054352	40.0	37.0	41.0	34.0	41.0
31	38.022174829448375	40.0	36.0	41.0	34.0	41.0
32	37.873603472144275	40.0	36.0	41.0	34.0	41.0
33	37.70478542298051	40.0	36.0	41.0	33.0	41.0
34	37.48589316671032	40.0	36.0	41.0	33.0	41.0
35	37.36355745126651	40.0	36.0	41.0	33.0	41.0
36	37.28172509696811	40.0	36.0	41.0	33.0	41.0
37	37.197458250707776	40.0	35.0	41.0	33.0	41.0
38	37.05584115937691	40.0	35.0	41.0	32.0	41.0
39	37.01895212082663	40.0	35.0	41.0	32.0	41.0
40	36.91718486923336	40.0	35.0	41.0	32.0	41.0
41	36.83538993028274	40.0	35.0	41.0	31.0	41.0
42	36.774310622217236	40.0	35.0	41.0	31.0	41.0
43	36.75036479964081	39.0	35.0	41.0	31.0	41.0
44	36.691989374041235	39.0	35.0	41.0	31.0	41.0
45	36.61476409623227	39.0	35.0	41.0	31.0	41.0
46	36.55499681969544	39.0	35.0	41.0	31.0	41.0
47	36.52044249884636	39.0	35.0	41.0	31.0	41.0
48	36.45497811202155	39.0	35.0	41.0	31.0	41.0
49	36.393165463139646	39.0	35.0	41.0	31.0	41.0
50	36.309105648470336	39.0	35.0	41.0	31.0	41.0
51	35.50155273693269	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	12.0
11	14.0
12	15.0
13	11.0
14	20.0
15	28.0
16	24.0
17	63.0
18	52.0
19	139.0
20	225.0
21	350.0
22	532.0
23	818.0
24	1355.0
25	2296.0
26	3641.0
27	4646.0
28	4791.0
29	4463.0
30	4611.0
31	5434.0
32	6535.0
33	9117.0
34	18247.0
35	27260.0
36	25769.0
37	38446.0
38	74454.0
39	167309.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54577767800352	19.245207717539067	25.012658859330767	16.19635574512665
2	33.59723625297764	24.35564535239022	25.3845674162208	16.662550978411346
3	32.202392087901124	22.707873436350255	29.41420037165912	15.675534104089497
4	28.187226400269388	24.77494668312942	28.81256157942655	18.225265337174644
5	24.795150970928276	28.96621394095858	27.198962347688354	19.03967274042479
6	26.308227634975868	33.28918322295806	25.659445504545964	14.743143637520111
7	77.80247190730971	5.918359711153515	11.57231763135905	4.706850750177723
8	78.99776755091605	5.478105785659944	10.985644978236738	4.538481685187263
9	71.89483792918521	8.076975842157118	13.614447312954441	6.413738915703221
10	36.46424963520036	28.573851660617848	20.373405170801064	14.588493533380726
11	27.787630486025368	24.39131465060301	28.62822863271847	19.192826230653147
12	25.879697185118665	21.2214863870493	32.99709407465609	19.90172235317594
13	23.691398211546378	22.38859580199798	33.622179818161406	20.297826168294232
14	21.576682755266212	24.953542609845226	31.96892031778102	21.500854317107542
15	21.155884810615984	23.61357428817301	34.480737331786834	20.749803569424177
16	23.3456803980993	23.1578553522655	32.529651663112205	20.96681258652299
17	22.82660480662501	23.200508848729747	30.644167570870906	23.328718773774334
18	23.55121537521358	23.125678153178434	31.138798468465094	22.18430800314289
19	24.500068594804254	23.952058467716792	30.378269166011897	21.169603771467056
20	24.441950087926067	24.535987328668888	29.94524887442162	21.076813708983426
21	24.693131789326646	24.640999738092564	30.340604382584402	20.325264089996384
22	22.77422331973909	23.188535937441536	30.115114553323107	23.922126189496264
23	22.1089784362879	24.520771753906786	30.416183385091234	22.954066424714085
24	21.78521096020254	24.362629550641675	31.030044524263857	22.82211496489193
25	22.55023010438882	24.741771741435002	29.80032676070391	22.90767139347227
26	21.042890460333496	25.78790486524239	29.99114503435976	23.178059640064355
27	22.912161235205346	24.51553360521819	30.12409423678926	22.448210922787194
28	21.1910552375251	25.139122734812485	31.011586286027864	22.658235741634552
29	22.62131926516257	24.327209688080718	30.026315461268876	23.025155585487834
30	22.89644678913957	24.31698282635537	30.0959080081316	22.690662376373457
31	23.175066412242302	24.9368304211721	28.539429540664248	23.348673625921354
32	23.235928711290704	25.81185068781881	28.874920492385975	22.07730010850451
33	23.487609283994964	25.41599630835235	27.995659819658027	23.100734587994662
34	22.285080006485327	24.980980531547374	29.730235342537508	23.00370411942979
35	23.023908407228646	24.859754804754246	28.94925231663362	23.167084471383497
36	23.799653284443945	26.858981554233548	27.625996183634527	21.715368977687984
37	23.360646537209565	27.271049251069456	28.147815567279032	21.22048864444195
38	22.963794415135755	27.539691448098676	27.30297701450468	22.193537122260885
39	24.895424102967038	24.550454596475475	27.93379977800227	22.62032152255522
40	23.15561043139896	24.57514872600741	30.63917885783415	21.630061984759482
41	20.907945772689292	26.288272782828848	29.161272620695676	23.642508823786184
42	21.983761739065365	26.611042516306853	28.391264763472645	23.013930981155138
43	22.940596899514848	24.343672441102008	28.766914855140246	23.9488158042429
44	23.00944113942206	24.58936655816216	28.891632681059104	23.50955962135668
45	23.443708609271525	23.775707461867523	28.851972412416906	23.928611516444047
46	22.78544792407179	24.930594529876153	29.063244409523453	23.2207131365286
47	21.926142103490854	25.20198051907559	30.403212731195673	22.468664646237887
48	22.047866701587658	25.186764944313488	29.60975792269989	23.15561043139896
49	22.39632830720495	24.240406081241193	30.51246554670059	22.850800064853267
50	21.561966051807786	23.924121674710968	30.84770706277048	23.666205210710768
51	21.937865579127227	23.60833613948442	29.275763584889187	25.178034696499168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	510.5
2	878.0
3	4252.0
4	7626.0
5	4868.0
6	2110.0
7	2032.5
8	1955.0
9	1926.0
10	1897.0
11	1861.0
12	1825.0
13	1757.5
14	1690.0
15	1644.0
16	1598.0
17	1520.5
18	1443.0
19	1413.5
20	1384.0
21	1434.0
22	1484.0
23	1603.5
24	1723.0
25	2277.0
26	3207.5
27	3584.0
28	4241.5
29	4899.0
30	5632.0
31	6365.0
32	6624.5
33	6884.0
34	8078.0
35	9272.0
36	9800.0
37	10328.0
38	11140.5
39	11953.0
40	13297.5
41	14642.0
42	17106.0
43	19570.0
44	21029.5
45	22489.0
46	26347.5
47	30206.0
48	33440.5
49	36675.0
50	36040.5
51	35406.0
52	32351.5
53	29297.0
54	26738.5
55	24180.0
56	22931.0
57	21682.0
58	20347.0
59	19012.0
60	18031.0
61	17050.0
62	15894.0
63	14738.0
64	13151.5
65	11565.0
66	9550.0
67	7535.0
68	6448.5
69	5362.0
70	4557.5
71	3753.0
72	3047.5
73	2342.0
74	2025.0
75	1240.0
76	772.0
77	583.0
78	394.0
79	415.0
80	436.0
81	283.5
82	131.0
83	99.0
84	67.0
85	39.0
86	11.0
87	10.5
88	10.0
89	5.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.71504103678049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6073981301396	25.21746121718234
2	7.923265931296318	5.659595357631456
3	3.6567524260455633	3.918031888726918
4	2.488788996141193	3.5554880451628175
5	1.9897001266253342	3.5531110836655575
6	1.758547376018283	3.7683955019769386
7	1.664355268163302	4.160976169156389
8	1.4147331843852113	4.0421802989130375
9	1.3315454823191504	4.280058138903132
>10	7.0732282836373574	36.47572911042254
>50	0.058790250375652255	1.443291176917305
>100	0.028695241254782646	1.940469242537069
>500	0.003499419665217396	0.7503914293567283
>1k	6.998839330434792E-4	1.2348213394477807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4940	1.232212120078323	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	751	0.18732617453012557	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	620	0.15465010413938465	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	605	0.15090856936181887	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	525	0.1309537172148015	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	501	0.12496726157069629	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	406	0.10127087464611316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16038712413165213	0.0
2	0.0	0.0	0.0	0.636809219141692	0.0
3	0.0	0.0	0.0	0.9186715057183124	0.0
4	0.0	0.0	0.0	1.2384480113742657	0.0
5	0.0	0.0	0.0	2.1179581197540562	0.0
6	0.0	0.0	0.0	2.829099163143388	0.0
7	0.0	0.0	0.0	3.4689016101071326	0.0
8	0.0	0.0	0.0	4.455918484428979	0.0
9	0.0	0.0	0.0	4.952295431586037	0.0
10	0.0	0.0	0.0	5.794639627842008	0.0
11	0.0	0.0	0.0	6.816826929072972	0.0
12	0.0	0.0	0.0	7.6449532931741935	0.0
13	0.0	0.0	0.0	7.997655304872725	0.0
14	0.0	0.0	0.0	8.15654581509335	0.0
15	0.0	0.0	0.0	8.417954378219278	0.0
16	0.0	0.0	0.0	8.87566879934149	0.0
17	0.0	0.0	0.0	9.461593145508287	0.0
18	0.0	0.0	0.0	10.109626968982676	0.0
19	0.0	0.0	0.0	10.510719497137726	0.0
20	0.0	0.0	0.0	10.898841371397214	0.0
21	0.0	0.0	0.0	11.337099811676083	0.0
22	0.0	0.0	0.0	11.810029807560394	0.0
23	0.0	0.0	0.0	12.293186665170053	0.0
24	0.0	0.0	0.0	12.676818697696461	0.0
25	0.0	0.0	0.0	13.035257729387261	0.0
26	0.0	0.0	0.0	13.366258839375911	0.0
27	0.0	0.0	0.0	13.69651164240905	0.0
28	0.0	0.0	0.0	14.05370349584066	0.0
29	0.0	0.0	0.0	14.411893091879623	0.0
30	0.0	0.0	0.0	14.846409997380926	0.0
31	0.0	0.0	0.0	15.220563475137501	0.0
32	0.0	0.0	0.0	15.571768872925007	0.0
33	0.0	0.0	0.0	15.924720320275377	0.0
34	0.0	0.0	0.0	16.266197727641213	0.0
35	0.0	0.0	0.0	16.639353462790435	0.0
36	0.0	0.0	0.0	16.985819583193027	0.0
37	0.0	0.0	0.0	17.326049812299672	0.0
38	0.0	0.0	0.0	17.677504645739017	0.0
39	0.0	0.0	0.0	18.03544480612614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	30	2.1619526E-6	45.000004	12
TGATGCG	30	2.1619526E-6	45.000004	1
GTAACGG	60	0.0	45.000004	2
TCGTTTA	25	3.8862454E-5	45.0	38
CGCATCG	25	3.8862454E-5	45.0	21
TGCATAG	35	1.2092823E-7	45.0	1
CCGTACT	20	7.027785E-4	45.0	29
TGCGACG	20	7.027785E-4	45.0	1
CAATGCG	25	3.8862454E-5	45.0	1
CGGTAAT	25	3.8862454E-5	45.0	6
TATCGAG	20	7.027785E-4	45.0	1
GTGATAG	55	6.002665E-11	40.909092	1
CGTTTTT	1965	0.0	40.76336	1
ACTGCGG	40	3.4507138E-7	39.375	2
GCGTAAG	40	3.4507138E-7	39.375	1
AATGCGG	75	0.0	39.000004	2
TCGGGAT	70	0.0	38.571426	5
CTACGAA	35	6.238117E-6	38.571426	11
TATTAGT	35	6.238117E-6	38.571426	30
CGTAAGG	70	0.0	38.571426	2
>>END_MODULE