##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551550_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630867
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.223826892197565	33.0	31.0	34.0	30.0	34.0
2	32.34547218351887	34.0	31.0	34.0	30.0	34.0
3	32.364306581260394	34.0	31.0	34.0	30.0	34.0
4	35.91639442227918	37.0	35.0	37.0	35.0	37.0
5	35.98698457836596	37.0	35.0	37.0	35.0	37.0
6	35.988373143626156	37.0	35.0	37.0	35.0	37.0
7	36.12513097055322	37.0	36.0	37.0	35.0	37.0
8	35.955933659551064	37.0	35.0	37.0	35.0	37.0
9	37.82495359560731	39.0	38.0	39.0	35.0	39.0
10	37.562254801725246	39.0	37.0	39.0	35.0	39.0
11	37.523100748652254	39.0	37.0	39.0	35.0	39.0
12	37.453678826123415	39.0	37.0	39.0	35.0	39.0
13	37.38386220867473	39.0	37.0	39.0	35.0	39.0
14	38.674641406191796	40.0	38.0	41.0	35.0	41.0
15	38.6690887302712	40.0	38.0	41.0	35.0	41.0
16	38.757566967363964	40.0	38.0	41.0	35.0	41.0
17	38.64696362307745	40.0	38.0	41.0	35.0	41.0
18	38.59065698475273	40.0	38.0	41.0	35.0	41.0
19	38.51650664878651	40.0	37.0	41.0	34.0	41.0
20	38.341579128405826	40.0	37.0	41.0	34.0	41.0
21	38.30629593876364	40.0	37.0	41.0	34.0	41.0
22	38.29629541567399	40.0	37.0	41.0	34.0	41.0
23	38.21570632161771	40.0	37.0	41.0	34.0	41.0
24	38.147902806772265	40.0	37.0	41.0	34.0	41.0
25	38.09872286868706	40.0	36.0	41.0	34.0	41.0
26	38.08418256145907	40.0	37.0	41.0	34.0	41.0
27	38.05310628072161	40.0	36.0	41.0	34.0	41.0
28	37.98502695496832	40.0	36.0	41.0	34.0	41.0
29	37.93464866604213	40.0	36.0	41.0	34.0	41.0
30	37.79983895179174	40.0	36.0	41.0	33.0	41.0
31	37.75057341721789	40.0	36.0	41.0	33.0	41.0
32	37.55661969955632	40.0	36.0	41.0	33.0	41.0
33	37.44825137469546	40.0	36.0	41.0	33.0	41.0
34	37.27511821033593	40.0	36.0	41.0	33.0	41.0
35	37.198531544683746	40.0	35.0	41.0	33.0	41.0
36	37.16030478690437	40.0	35.0	41.0	32.0	41.0
37	37.06975796800276	40.0	35.0	41.0	32.0	41.0
38	36.939243929386066	40.0	35.0	41.0	31.0	41.0
39	36.914674566905546	40.0	35.0	41.0	32.0	41.0
40	36.88642297029326	39.0	35.0	41.0	31.0	41.0
41	36.74213899284635	39.0	35.0	41.0	31.0	41.0
42	36.69121859282543	39.0	35.0	41.0	31.0	41.0
43	36.6619017954656	39.0	35.0	41.0	31.0	41.0
44	36.673243330210646	39.0	35.0	41.0	31.0	41.0
45	36.533129803904785	39.0	35.0	41.0	31.0	41.0
46	36.48182738992529	39.0	35.0	41.0	31.0	41.0
47	36.361120489738724	39.0	35.0	41.0	31.0	41.0
48	36.34941120711655	39.0	35.0	41.0	31.0	41.0
49	36.355103373611236	39.0	35.0	41.0	31.0	41.0
50	36.2499782045978	39.0	35.0	41.0	31.0	41.0
51	35.51591856920714	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	7.0
11	14.0
12	18.0
13	12.0
14	14.0
15	30.0
16	53.0
17	80.0
18	142.0
19	309.0
20	582.0
21	1017.0
22	1660.0
23	2253.0
24	3018.0
25	4105.0
26	5368.0
27	6162.0
28	6603.0
29	6819.0
30	7822.0
31	9971.0
32	12594.0
33	17195.0
34	32018.0
35	46983.0
36	43541.0
37	62822.0
38	119627.0
39	239742.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06195442145492	18.508021500569853	23.034173605530167	13.395850472445064
2	31.7902188575405	27.867680509521026	23.134987247708313	17.207113385230166
3	29.7603139806013	27.987515593619573	26.0950406345553	16.157129791223824
4	26.912962637132708	23.123415870540065	31.268555812873394	18.695065679453833
5	28.215931408680433	26.874761241275895	24.644180152076427	20.265127197967242
6	24.14280664545776	35.30284513217524	25.482074668670258	15.07227355369674
7	73.75120270992143	5.7435243878662225	15.228883425508071	5.276389476704281
8	73.00096533817747	9.486944157801883	11.60196998733489	5.910120516685767
9	66.95975538425691	7.286955887691067	13.434527404349886	12.318761323702143
10	38.10945888753097	24.3374594011099	20.776645473610127	16.776436237749003
11	30.524817433785568	22.741718936003945	26.620190943574478	20.113272686636012
12	25.698126546482857	20.395107051090008	30.87037362867292	23.036392773754212
13	24.584262610027153	21.517213612377887	34.20055257288779	19.697971204707173
14	20.27828369529552	26.401127337457815	30.332700870389477	22.98788809685718
15	18.75260554126306	23.051134391242527	36.24646716344333	21.94979290405109
16	21.46189291879271	23.537766280372885	30.653370678764304	24.346970122070104
17	21.399280672471377	24.46664669415265	30.123782033297037	24.01029060007894
18	22.745681736404027	23.338199652224638	30.00901933371059	23.90709927766074
19	22.547700228415817	25.260157846265535	27.62404754092384	24.568094384394808
20	24.585372194139175	24.568094384394808	29.66774296325533	21.178790458210685
21	22.81241529514145	28.06899076984531	28.52820008020708	20.590393854806162
22	21.581410978859253	22.703676052163136	29.427438746994216	26.2874742219834
23	22.343061215755462	26.03258690024997	29.535543941908514	22.08880794208605
24	23.410956667570186	23.57897940453376	28.534065024799204	24.475998903096848
25	21.28372541280492	27.818383272543972	26.847338662507315	24.050552652143793
26	19.859811973046618	23.895527900492496	29.852250949883256	26.39240917657763
27	23.406201307090083	24.308610214197287	27.999403994819826	24.285784483892805
28	18.94694127288319	26.524132661876433	29.91105890782051	24.617867157419866
29	21.621514518908107	24.051820748271822	28.049969327924902	26.276695404895168
30	21.08368324860866	25.918933784775554	29.78345673493779	23.21392623167799
31	22.917667273767687	26.286681661903383	24.852623453120863	25.94302761120807
32	22.527410690367383	28.043153311236757	25.07327217939756	24.3561638189983
33	21.62516029527618	24.685393276237306	25.044264480468943	28.645181948017566
34	20.23691205911864	26.949103376781476	26.347391764032675	26.466592800067207
35	20.03671138290638	24.774318517215196	26.832438533002996	28.356531566875425
36	19.833657490406058	28.767870248404183	24.423372913783727	26.97509934740603
37	19.85616619667854	28.693211088866594	27.90746702553787	23.543155688916997
38	19.813367952357627	28.430398166333	24.102703105408903	27.653530775900464
39	21.85246652622502	27.421627696487533	25.16869641303159	25.557209364255858
40	21.023924218575388	23.49338291589194	27.74293155292637	27.739761312606305
41	18.369640510598906	26.36324296563301	25.962524589176482	29.3045919345916
42	20.084899035771407	24.37914806131879	28.38030836927593	27.155644533633872
43	21.634988040268393	23.271941629535227	27.079558765952257	28.013511564244126
44	21.672713900077195	24.00791291983889	28.268715910009558	26.050657270074357
45	21.029472139135507	23.268612877199157	27.263432704516166	28.43848227914917
46	23.457717712291178	25.690993505762705	25.90260704712721	24.94868173481891
47	19.198658354296548	24.31716986306147	31.73188643565125	24.75228534699073
48	20.5122474309165	24.25138737642007	28.141589273174855	27.09477591948858
49	20.5639223481336	22.865199796470574	30.844219146032366	25.726658709363466
50	20.52762309646883	22.045851185749136	30.21571900257899	27.210806715203045
51	20.112955662604005	22.114011352630587	27.645288151068293	30.12774483369712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	615.5
2	1021.0
3	4433.0
4	7845.0
5	5046.0
6	2247.0
7	2128.0
8	2009.0
9	1987.0
10	1965.0
11	1906.5
12	1848.0
13	1797.5
14	1747.0
15	1783.5
16	1820.0
17	1781.5
18	1743.0
19	1673.5
20	1604.0
21	1745.0
22	1886.0
23	2001.5
24	2117.0
25	2530.0
26	3785.0
27	4627.0
28	5504.5
29	6382.0
30	7153.5
31	7925.0
32	8832.0
33	9739.0
34	10819.0
35	11899.0
36	12998.0
37	14097.0
38	15693.0
39	17289.0
40	19102.0
41	20915.0
42	23954.5
43	26994.0
44	31085.5
45	35177.0
46	55024.5
47	74872.0
48	68304.0
49	61736.0
50	61190.5
51	60645.0
52	53357.0
53	46069.0
54	41553.0
55	37037.0
56	34688.0
57	32339.0
58	30565.5
59	28792.0
60	27964.0
61	27136.0
62	24668.5
63	22201.0
64	19899.0
65	17597.0
66	14875.0
67	12153.0
68	10189.5
69	8226.0
70	6951.0
71	5676.0
72	4812.5
73	3949.0
74	3443.5
75	2371.0
76	1804.0
77	1313.5
78	823.0
79	617.5
80	412.0
81	349.5
82	287.0
83	174.0
84	61.0
85	42.0
86	23.0
87	28.5
88	34.0
89	19.0
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	630867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.99243302990107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74936181572484	23.274290858576194
2	8.935445320531205	5.717332720188749
3	3.3339486743826265	3.1998338907094084
4	1.9225051947343512	2.4602247476870254
5	1.4157806488662217	2.2647133796941237
6	1.1101039707747669	2.130895616474339
7	0.9709028515631759	2.174308112002242
8	0.8573509877093953	2.194299524592989
9	0.7817046528565196	2.2507770380106837
>10	7.79008687356077	43.35608735635573
>50	0.0800833351936764	1.7689358486657207
>100	0.044767081785079404	2.712124295493484
>500	0.0019896480793368623	0.42234218624888265
>1k	0.0044767081785079404	2.6876176275649506
>5k	0.0014922360595026468	3.3862167977354836
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	7276	1.153333428440543	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	7206	1.1422375873203068	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	6797	1.0774061727749271	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4777	0.7572119004481135	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3135	0.49693517017057476	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1606	0.25457029770141726	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	1307	0.2071752049164087	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1300	0.2060656208043851	Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	1298	0.20574859677237833	Illumina PCR Primer Index 11 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1259	0.19956662814824677	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	1123	0.17800899397178802	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	1084	0.17182702534765648	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	758	0.12015210813055682	No Hit
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	712	0.11286055539440167	Illumina PCR Primer Index 11 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	660	0.10461793056222626	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20495603669236145	0.0
2	0.0	0.0	0.0	1.1095841120236118	0.0
3	0.0	0.0	0.0	1.488110806239667	0.0
4	0.0	0.0	0.0	2.150056985069753	0.0
5	0.0	0.0	0.0	4.5559523639689505	0.0
6	0.0	0.0	0.0	5.567100514054468	0.0
7	0.0	0.0	0.0	6.710289173470795	0.0
8	0.0	0.0	0.0	8.244209952335437	0.0
9	0.0	0.0	0.0	8.87968462449296	0.0
10	0.0	0.0	0.0	11.1324573959329	0.0
11	0.0	0.0	0.0	12.456032729561064	0.0
12	0.0	0.0	0.0	14.583422496342335	0.0
13	0.0	0.0	0.0	15.10714619721748	0.0
14	0.0	0.0	0.0	15.326843851398156	0.0
15	0.0	0.0	0.0	15.869430482177702	0.0
16	0.0	0.0	0.0	16.54643530252811	0.0
17	0.0	0.0	0.0	17.301428034752174	0.0
18	0.0	0.0	0.0	18.103815859761248	0.0
19	0.0	0.0	0.0	18.819180587984473	0.0
20	0.0	0.0	0.0	19.36414489900407	0.0
21	0.0	0.0	0.0	19.954602158616634	0.0
22	0.0	0.0	0.0	20.56566598030964	0.0
23	0.0	0.0	0.0	21.149148711218054	0.0
24	0.0	0.0	0.0	21.583313123051294	0.0
25	0.0	0.0	0.0	21.98720173982789	0.0
26	0.0	0.0	0.0	22.374763618956134	0.0
27	0.0	0.0	0.0	22.82937608085381	0.0
28	0.0	0.0	0.0	23.246896731006693	0.0
29	0.0	0.0	0.0	23.658076900519443	0.0
30	0.0	0.0	0.0	24.139794917153694	0.0
31	0.0	0.0	0.0	24.56429009601073	0.0
32	0.0	0.0	0.0	25.00527052453211	0.0
33	0.0	0.0	0.0	25.404403780828606	0.0
34	1.5851201600337314E-4	0.0	0.0	25.805597693333144	0.0
35	1.5851201600337314E-4	0.0	0.0	26.234848232670277	0.0
36	1.5851201600337314E-4	0.0	0.0	26.639846433558898	0.0
37	1.5851201600337314E-4	0.0	0.0	27.045954218559537	0.0
38	1.5851201600337314E-4	0.0	0.0	27.464108916776436	0.0
39	1.5851201600337314E-4	0.0	0.0	27.884641295233386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.0	25
CGTATTT	20	7.030864E-4	45.0	32
GTTAGCG	40	6.8066583E-9	45.0	1
ACGATAT	20	7.030864E-4	45.0	23
TACGCCG	25	3.8887985E-5	45.0	29
GCGTATG	35	1.2107557E-7	45.0	1
CGTTTTT	1905	0.0	41.220474	1
CGACGGT	55	6.002665E-11	40.909092	28
CGGTCTA	55	6.002665E-11	40.909092	31
CGAATAT	50	1.0804797E-9	40.5	14
TACGGCT	795	0.0	40.18868	7
CGTATGG	45	1.925946E-8	40.0	2
ATAACGG	45	1.925946E-8	40.0	2
CGATGAA	630	0.0	39.642857	19
GCGCCCA	40	3.4549157E-7	39.375	33
GGCGTAG	35	6.2438085E-6	38.571426	1
GCAAACG	35	6.2438085E-6	38.571426	1
CCGATGA	640	0.0	38.320312	18
CGGGTCA	30	1.1393383E-4	37.499996	6
ACTTGCG	55	2.744855E-9	36.81818	14
>>END_MODULE