FastQCFastQC Report
Sat 18 Jun 2016
SRR3551547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229523
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG30881.3453989360543388No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC29261.2748177742535607No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC24011.046082527676965No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.5973257582028817No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC9840.42871520501213384Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT5840.25444073142996565No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGCAGGT5770.2513909281422777No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA5150.2243783847370416No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT3730.16251094661537188No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC3710.16163957424746103No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT3430.14944036109670927Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3160.1376768341299129No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC2940.12809173808289365Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC2830.12329919005938403No Hit
GAGATGGCTTTGTAAAAAGAGGGTTTCTTGGTTTCAGAATTATTGCTGGGG2830.12329919005938403No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA2460.10717880125303346No Hit
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC2330.101514880861613Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGT207.021477E-445.0000044
TTGGACG207.021477E-445.0000041
CTATAGG406.7739165E-945.0000042
GTGATAG253.8810198E-545.0000041
TATTGGA207.021477E-445.00000425
TTGAGCG207.021477E-445.0000041
GGCTTAA253.8810198E-545.0000048
GTTACGG207.021477E-445.0000042
TTGGTCG207.021477E-445.00000412
GCGATTA207.021477E-445.0000049
CGTAAGG207.021477E-445.0000042
TTACGGG502.1827873E-1145.0000043
ACTTTCG207.021477E-445.00000423
ACATAGA207.021477E-445.00000435
GGCCTAT207.021477E-445.0000048
GTTATAG207.021477E-445.0000041
TGACTAT207.021477E-445.00000423
GTACGAG351.2062446E-745.01
GTCGAAT600.044.99999643
GATGAAT4250.042.88235520
Produced by FastQC (version 0.11.5)