##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229523
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28622839541136	33.0	31.0	34.0	30.0	34.0
2	32.41438548642184	34.0	31.0	34.0	30.0	34.0
3	32.427120593578856	34.0	31.0	34.0	30.0	34.0
4	35.96328472527808	37.0	35.0	37.0	35.0	37.0
5	36.018320604035324	37.0	35.0	37.0	35.0	37.0
6	36.023993238150425	37.0	35.0	37.0	35.0	37.0
7	36.130784278699736	37.0	36.0	37.0	35.0	37.0
8	35.949883018259605	37.0	35.0	37.0	35.0	37.0
9	37.81102111770934	39.0	38.0	39.0	35.0	39.0
10	37.58445558832884	39.0	37.0	39.0	35.0	39.0
11	37.548633470284024	39.0	37.0	39.0	35.0	39.0
12	37.40333212793489	39.0	37.0	39.0	35.0	39.0
13	37.28391490177455	39.0	37.0	39.0	34.0	39.0
14	38.59093859874609	40.0	38.0	41.0	34.0	41.0
15	38.5916661946733	40.0	38.0	41.0	34.0	41.0
16	38.726458786265425	40.0	38.0	41.0	35.0	41.0
17	38.59210188085726	40.0	38.0	41.0	35.0	41.0
18	38.55525154341831	40.0	38.0	41.0	35.0	41.0
19	38.47312905460455	40.0	37.0	41.0	34.0	41.0
20	38.23410290036293	40.0	37.0	41.0	34.0	41.0
21	38.23889980524827	40.0	37.0	41.0	34.0	41.0
22	38.17748112389608	40.0	37.0	41.0	34.0	41.0
23	38.060813077556496	40.0	36.0	41.0	34.0	41.0
24	37.99800455727748	40.0	36.0	41.0	34.0	41.0
25	37.97674307150046	40.0	36.0	41.0	34.0	41.0
26	37.92227794164419	40.0	36.0	41.0	34.0	41.0
27	37.85550903395303	40.0	36.0	41.0	34.0	41.0
28	37.785943020960865	40.0	36.0	41.0	33.0	41.0
29	37.698723003794825	40.0	36.0	41.0	33.0	41.0
30	37.614578931087514	40.0	36.0	41.0	33.0	41.0
31	37.564244106255146	40.0	36.0	41.0	33.0	41.0
32	37.337913847413986	40.0	35.0	41.0	33.0	41.0
33	37.19022930163862	40.0	35.0	41.0	33.0	41.0
34	36.94163547879733	40.0	35.0	41.0	31.0	41.0
35	36.891344222583356	40.0	35.0	41.0	31.0	41.0
36	36.843170401223404	40.0	35.0	41.0	31.0	41.0
37	36.610361488826825	39.0	35.0	41.0	31.0	41.0
38	36.51221010530535	39.0	35.0	41.0	30.0	41.0
39	36.486761675300514	39.0	35.0	41.0	31.0	41.0
40	36.41804089350523	39.0	35.0	41.0	30.0	41.0
41	36.30410895640088	39.0	35.0	41.0	30.0	41.0
42	36.182382593465576	39.0	35.0	41.0	30.0	41.0
43	36.143414821172605	39.0	35.0	41.0	30.0	41.0
44	36.18434318129338	39.0	35.0	41.0	30.0	41.0
45	36.10981034580412	39.0	35.0	41.0	30.0	41.0
46	36.06822409954558	39.0	35.0	41.0	30.0	41.0
47	35.929488547988655	38.0	35.0	41.0	30.0	41.0
48	35.93609355053742	38.0	35.0	41.0	30.0	41.0
49	35.922195161269244	38.0	35.0	41.0	30.0	41.0
50	35.73796961524553	38.0	35.0	41.0	29.0	41.0
51	34.96375526635675	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	7.0
12	4.0
13	9.0
14	11.0
15	4.0
16	16.0
17	25.0
18	70.0
19	148.0
20	286.0
21	588.0
22	964.0
23	1204.0
24	1590.0
25	1880.0
26	2264.0
27	2539.0
28	2477.0
29	2540.0
30	2879.0
31	3611.0
32	4636.0
33	6283.0
34	12075.0
35	18455.0
36	15688.0
37	23043.0
38	41657.0
39	84460.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71567991007437	18.99461056190447	22.330659672451127	12.95904985557003
2	30.649651668895928	29.84450360094631	23.343194363963523	16.162650366194235
3	29.55085111296036	29.114293556637026	25.8610248210419	15.473830509360717
4	27.06831123678237	23.540124519111373	31.650422833441528	17.741141410664728
5	29.512075042588325	26.880530491497584	24.295604362090074	19.311790103824016
6	24.80884268678956	37.465962016878485	24.109130675357154	13.616064620974806
7	73.47019688658652	6.100913633927754	15.83806415914745	4.590825320338267
8	72.4384920029801	11.288629026284948	10.879519699550807	5.393359271184151
9	66.20295133821011	8.11639792090553	12.771269110285244	12.909381630599112
10	40.57414725321645	24.515190198803605	19.542268095136436	15.368394452843507
11	33.994850189305645	22.653503134762094	24.44635178173865	18.90529489419361
12	27.244764141284318	20.602292580699974	29.58396326294097	22.56898001507474
13	26.05621223145393	22.66134548607329	32.91478413928016	18.36765814319262
14	20.93472113905796	29.227571964465437	28.50302584054757	21.334681055929035
15	18.991125072432826	23.31226064490269	35.75850786195719	21.938106420707292
16	20.747376079957128	23.259106930460128	30.94635396016957	25.047163029413174
17	21.493706513072762	25.175690453680023	29.846682031866088	23.483921001381127
18	22.85261172082972	23.018608156916738	29.691142064194004	24.43763805805954
19	23.138421857504476	25.02668577876727	27.71138404430057	24.123508319427682
20	26.730218758032965	23.79543662290925	29.557822091903645	19.91652252715414
21	23.855997002479054	28.069518087511923	27.566300545043415	20.508184364965604
22	22.145493044270072	23.402883371165416	29.3334437071666	25.118179877397907
23	23.916121695864902	26.514118410791077	27.545387608213556	22.024372285130468
24	22.736283509713623	24.777908967728724	28.36970586825721	24.11610165430044
25	21.037543078471437	29.737760485877235	27.11667240320142	22.108024032449908
26	20.49685652418276	25.349964927262192	30.173446669832654	23.979731878722394
27	24.834112485458974	25.436230791685364	27.51009702731317	22.219559695542497
28	19.432039490595717	28.002422415182792	30.027927484391544	22.537610609829954
29	23.121430096330215	25.021893230743757	28.55443681025431	23.302239862671716
30	21.230987744147644	28.365349006417656	28.377983905752362	22.025679343682334
31	24.23591535488818	27.69047110747071	23.306161038327314	24.767452499313794
32	27.56281505557177	27.460864488526205	24.70689211974399	20.269428336158033
33	24.147035373361277	25.487641761392105	24.40714002518266	25.95818284006396
34	22.746739978128552	28.968338685011958	25.488077447576057	22.796843889283426
35	25.283740627300965	24.899901099236242	26.38341255560445	23.432945717858342
36	24.2895047555147	30.693655973475426	23.93267777085521	21.084161500154668
37	24.36749258244272	27.755388348880068	27.536673884534444	20.340445184142766
38	22.945412878012224	28.48864819647704	23.517033151361737	25.048905774148995
39	26.243992976738717	27.322751968212337	24.874631300566826	21.558623754482124
40	24.312596123264335	24.604941552698424	27.158498276861142	23.9239640471761
41	23.029500311515623	27.934891056669702	24.94259834526387	24.093010286550804
42	24.37489924756996	25.036706560998244	28.211116097297438	22.377278094134358
43	25.457143728515224	24.39276238111213	26.49756233580077	23.652531554571873
44	22.87831720568309	24.801871707846274	28.096094944733206	24.223716141737427
45	22.55111688153257	24.467700404752467	26.406068237170132	26.575114476544837
46	25.954261664408364	26.748081891575136	25.555173119905195	21.74248332411131
47	21.458415932172375	25.663658979710096	31.236520958683876	21.641404129433653
48	23.375435141576226	25.48328489955255	26.63436779756277	24.506912161308453
49	23.236451248894447	23.175019496956732	30.59257677879777	22.995952475351054
50	22.788565851788274	24.087782052343336	28.742217555539096	24.38143454032929
51	22.19821107252868	24.61757645203313	26.20042435834317	26.98378811709502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	317.0
2	500.0
3	1422.5
4	2345.0
5	1613.0
6	881.0
7	856.0
8	831.0
9	788.0
10	745.0
11	756.5
12	768.0
13	776.5
14	785.0
15	815.5
16	846.0
17	825.5
18	805.0
19	792.0
20	779.0
21	771.5
22	764.0
23	885.0
24	1006.0
25	1224.0
26	1604.0
27	1766.0
28	1952.5
29	2139.0
30	2683.0
31	3227.0
32	3504.5
33	3782.0
34	4345.5
35	4909.0
36	5271.0
37	5633.0
38	6007.0
39	6381.0
40	7609.0
41	8837.0
42	9805.0
43	10773.0
44	12190.0
45	13607.0
46	20617.0
47	27627.0
48	23947.5
49	20268.0
50	19237.5
51	18207.0
52	16586.5
53	14966.0
54	13851.5
55	12737.0
56	12426.0
57	12115.0
58	11534.0
59	10953.0
60	10378.0
61	9803.0
62	9229.5
63	8656.0
64	7504.0
65	6352.0
66	5300.5
67	4249.0
68	3832.5
69	3416.0
70	2926.0
71	2436.0
72	2113.0
73	1790.0
74	1483.5
75	838.5
76	500.0
77	418.0
78	336.0
79	238.5
80	141.0
81	109.0
82	77.0
83	51.5
84	26.0
85	14.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	229523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.899970809025675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7358203223286	23.930063653751475
2	7.70728218982162	5.0713871812410956
3	3.1809092474143528	3.1395546415827607
4	1.9797915590692994	2.605403380053415
5	1.5189437580284189	2.498660264984337
6	1.3772463019612518	2.718681787881825
7	1.1786050084091482	2.7143249260422704
8	1.0991484909883065	2.892956261463993
9	1.0607445075682331	3.140861700134627
>10	8.046296664150544	42.02933910762756
>50	0.07018659038840994	1.5959184918287055
>100	0.0344311575490313	2.2402983578987725
>500	0.00529710116138943	1.1589252493214188
>1k	0.00529710116138943	4.263624996187746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	3088	1.3453989360543388	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	2926	1.2748177742535607	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	2401	1.046082527676965	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1371	0.5973257582028817	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	984	0.42871520501213384	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	584	0.25444073142996565	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGCAGGT	577	0.2513909281422777	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA	515	0.2243783847370416	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	373	0.16251094661537188	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC	371	0.16163957424746103	No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	343	0.14944036109670927	Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	316	0.1376768341299129	No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	294	0.12809173808289365	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC	283	0.12329919005938403	No Hit
GAGATGGCTTTGTAAAAAGAGGGTTTCTTGGTTTCAGAATTATTGCTGGGG	283	0.12329919005938403	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	246	0.10717880125303346	No Hit
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	233	0.101514880861613	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.356861839554206E-4	0.0	0.0	0.16163957424746103	0.0
2	4.356861839554206E-4	0.0	0.0	0.9253974547213133	0.0
3	4.356861839554206E-4	0.0	0.0	1.215128767051668	0.0
4	4.356861839554206E-4	0.0	0.0	1.7157321924164464	0.0
5	4.356861839554206E-4	0.0	0.0	3.7307807932102666	0.0
6	4.356861839554206E-4	0.0	0.0	4.362090073761671	0.0
7	4.356861839554206E-4	0.0	0.0	5.202528722611677	0.0
8	4.356861839554206E-4	0.0	0.0	6.271266931854324	0.0
9	4.356861839554206E-4	0.0	0.0	6.704338998706012	0.0
10	4.356861839554206E-4	0.0	0.0	8.819159735625623	0.0
11	4.356861839554206E-4	0.0	0.0	9.794225415317856	0.0
12	4.356861839554206E-4	0.0	0.0	11.882469295016186	0.0
13	4.356861839554206E-4	0.0	0.0	12.258902157953669	0.0
14	4.356861839554206E-4	0.0	0.0	12.438404865743303	0.0
15	4.356861839554206E-4	0.0	0.0	12.911124375334934	0.0
16	4.356861839554206E-4	0.0	0.0	13.443532892128458	0.0
17	4.356861839554206E-4	0.0	0.0	14.04173002269925	0.0
18	4.356861839554206E-4	0.0	0.0	14.67347498943461	4.356861839554206E-4
19	4.356861839554206E-4	0.0	0.0	15.301734466698326	4.356861839554206E-4
20	4.356861839554206E-4	0.0	0.0	15.69995163883358	4.356861839554206E-4
21	4.356861839554206E-4	0.0	0.0	16.10906096556772	4.356861839554206E-4
22	4.356861839554206E-4	0.0	0.0	16.559560479777627	4.356861839554206E-4
23	4.356861839554206E-4	0.0	0.0	16.95516353480915	4.356861839554206E-4
24	4.356861839554206E-4	0.0	0.0	17.290641896454822	4.356861839554206E-4
25	4.356861839554206E-4	0.0	0.0	17.58080889496913	4.356861839554206E-4
26	4.356861839554206E-4	0.0	0.0	17.883175106634194	4.356861839554206E-4
27	4.356861839554206E-4	0.0	0.0	18.223010330119422	4.356861839554206E-4
28	4.356861839554206E-4	0.0	0.0	18.524940855600526	4.356861839554206E-4
29	4.356861839554206E-4	0.0	0.0	18.858240786326427	4.356861839554206E-4
30	4.356861839554206E-4	0.0	0.0	19.20417561638703	4.356861839554206E-4
31	4.356861839554206E-4	0.0	0.0	19.483450460302453	4.356861839554206E-4
32	4.356861839554206E-4	0.0	0.0	19.790609219991026	4.356861839554206E-4
33	4.356861839554206E-4	0.0	0.0	20.082954649425112	4.356861839554206E-4
34	4.356861839554206E-4	0.0	0.0	20.382706743986443	4.356861839554206E-4
35	4.356861839554206E-4	0.0	0.0	20.714699616160473	4.356861839554206E-4
36	4.356861839554206E-4	0.0	0.0	21.001816811387094	4.356861839554206E-4
37	4.356861839554206E-4	0.0	0.0	21.28849832042976	4.356861839554206E-4
38	4.356861839554206E-4	0.0	0.0	21.574744143288473	4.356861839554206E-4
39	4.356861839554206E-4	0.0	0.0	21.858811535227407	4.356861839554206E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGGT	20	7.021477E-4	45.000004	4
TTGGACG	20	7.021477E-4	45.000004	1
CTATAGG	40	6.7739165E-9	45.000004	2
GTGATAG	25	3.8810198E-5	45.000004	1
TATTGGA	20	7.021477E-4	45.000004	25
TTGAGCG	20	7.021477E-4	45.000004	1
GGCTTAA	25	3.8810198E-5	45.000004	8
GTTACGG	20	7.021477E-4	45.000004	2
TTGGTCG	20	7.021477E-4	45.000004	12
GCGATTA	20	7.021477E-4	45.000004	9
CGTAAGG	20	7.021477E-4	45.000004	2
TTACGGG	50	2.1827873E-11	45.000004	3
ACTTTCG	20	7.021477E-4	45.000004	23
ACATAGA	20	7.021477E-4	45.000004	35
GGCCTAT	20	7.021477E-4	45.000004	8
GTTATAG	20	7.021477E-4	45.000004	1
TGACTAT	20	7.021477E-4	45.000004	23
GTACGAG	35	1.2062446E-7	45.0	1
GTCGAAT	60	0.0	44.999996	43
GATGAAT	425	0.0	42.882355	20
>>END_MODULE