FastQCFastQC Report
Sat 18 Jun 2016
SRR3551545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526741
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG72461.3756286296301217No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC66851.2691246741757334No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC63721.2097026812038554No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.7214171670707236No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC27400.5201797467825744No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCT15470.2936927256469498No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGTTTGTT13270.2519264686060132No Hit
GAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT12470.23673873877294535No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTA10190.1934537087487019No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT9870.1873786168154748Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC9810.18623953707799468No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTC9760.18529030396342794No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7680.1458022063974515No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCC6260.11884398594375603No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6030.11447751361674903No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.887918E-545.01
ACCGGTT253.887918E-545.039
TAACGCG207.0298027E-445.01
GTGTCGA207.0298027E-445.016
ATGCGCT207.0298027E-445.018
CGATCGA900.045.041
TATCGTG207.0298027E-445.01
CGATCAC302.1632532E-644.99999634
ATAGGGT1050.042.8571474
CCGATCG950.042.6315840
TATTAGT700.041.78571330
CGATGAA9450.041.66666819
TCGAATG1300.041.5384644
ATTGCGG603.6379788E-1241.2499962
ATGAATG10100.041.2128721
GATGAAT10100.041.2128720
ACGTAGG501.0786607E-940.52
CCGATGA9350.040.4278118
GGTAATC451.9248546E-840.08
TACGAGG451.9248546E-840.02