##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551545_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526741
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.210374738248966	33.0	31.0	34.0	30.0	34.0
2	32.32220389147608	34.0	31.0	34.0	30.0	34.0
3	32.34850144568203	34.0	31.0	34.0	30.0	34.0
4	35.89522175034789	37.0	35.0	37.0	35.0	37.0
5	35.95977150060466	37.0	35.0	37.0	35.0	37.0
6	35.96363487938095	37.0	35.0	37.0	35.0	37.0
7	36.070537512743456	37.0	35.0	37.0	35.0	37.0
8	35.86812304339324	37.0	35.0	37.0	35.0	37.0
9	37.71355561841588	39.0	38.0	39.0	35.0	39.0
10	37.485802320305424	39.0	37.0	39.0	35.0	39.0
11	37.48076189246708	39.0	37.0	39.0	35.0	39.0
12	37.44908598343398	39.0	37.0	39.0	35.0	39.0
13	37.376452563973565	39.0	37.0	39.0	34.0	39.0
14	38.7063300559478	40.0	38.0	41.0	35.0	41.0
15	38.691757429172974	40.0	38.0	41.0	35.0	41.0
16	38.789528439973346	40.0	38.0	41.0	35.0	41.0
17	38.66616040900556	40.0	38.0	41.0	35.0	41.0
18	38.606542874012085	40.0	38.0	41.0	35.0	41.0
19	38.53089658864603	40.0	38.0	41.0	34.0	41.0
20	38.30234783318557	40.0	37.0	41.0	34.0	41.0
21	38.31423413024618	40.0	37.0	41.0	34.0	41.0
22	38.26347863561029	40.0	37.0	41.0	34.0	41.0
23	38.15213169280538	40.0	37.0	41.0	34.0	41.0
24	38.109146620445344	40.0	37.0	41.0	34.0	41.0
25	38.09461765839379	40.0	37.0	41.0	34.0	41.0
26	38.044205786145376	40.0	37.0	41.0	34.0	41.0
27	37.97327149396003	40.0	37.0	41.0	34.0	41.0
28	37.89555018500553	40.0	36.0	41.0	33.0	41.0
29	37.810007195187005	40.0	36.0	41.0	33.0	41.0
30	37.69398433005974	40.0	36.0	41.0	33.0	41.0
31	37.68954571601603	40.0	36.0	41.0	33.0	41.0
32	37.3533994885532	40.0	36.0	41.0	32.0	41.0
33	37.24229744789185	40.0	36.0	41.0	32.0	41.0
34	37.1137769795782	40.0	36.0	41.0	31.0	41.0
35	37.0129874074735	40.0	35.0	41.0	31.0	41.0
36	36.881038309150036	40.0	35.0	41.0	31.0	41.0
37	36.69338061779888	40.0	35.0	41.0	31.0	41.0
38	36.59230247882736	39.0	35.0	41.0	30.0	41.0
39	36.49340947448556	39.0	35.0	41.0	30.0	41.0
40	36.508151064754784	39.0	35.0	41.0	30.0	41.0
41	36.46950588619455	39.0	35.0	41.0	30.0	41.0
42	36.359425220364464	39.0	35.0	41.0	30.0	41.0
43	36.26180988379488	39.0	35.0	41.0	30.0	41.0
44	36.328937751190814	39.0	35.0	41.0	30.0	41.0
45	36.22128142673534	39.0	35.0	41.0	30.0	41.0
46	36.126211933379025	39.0	35.0	41.0	29.0	41.0
47	35.99987849816134	39.0	35.0	41.0	29.0	41.0
48	36.00928539832669	39.0	35.0	41.0	29.0	41.0
49	35.93927376072871	39.0	35.0	41.0	29.0	41.0
50	35.80472376367133	39.0	35.0	41.0	29.0	41.0
51	35.09914170341781	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	16.0
10	11.0
11	13.0
12	17.0
13	16.0
14	19.0
15	38.0
16	37.0
17	87.0
18	142.0
19	308.0
20	814.0
21	1472.0
22	2225.0
23	2901.0
24	3452.0
25	4229.0
26	5122.0
27	5593.0
28	5665.0
29	5907.0
30	6943.0
31	8451.0
32	10516.0
33	14097.0
34	25386.0
35	36383.0
36	35614.0
37	53871.0
38	99879.0
39	197240.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.087200350836554	17.686301237230442	20.894329471220203	13.3321689407128
2	32.40947638402935	29.24302456045761	21.66928338595249	16.67821566956056
3	30.573469693834348	28.682217636371576	24.99065005382152	15.753662615972555
4	26.83178260283517	22.608074936258994	32.02864405846517	18.53149840244067
5	28.7139220223981	26.019808596634782	24.02072365735722	21.245545723609897
6	24.476735245595084	37.204622385574694	24.533119692600348	13.785522676229874
7	71.74246925908558	6.2263996916890845	16.902994071089967	5.128136978135364
8	69.94841867255445	11.643103536652738	12.0848766281721	6.32360116262072
9	64.12069688898339	7.832122428290185	13.761602001742792	14.285578680983631
10	37.123937570836524	23.366322348174908	21.969051203532665	17.540688877455903
11	29.671318541750118	22.870063275879417	26.536191410959088	20.922426771411377
12	24.931038214226724	20.29365475632237	31.023406190138985	23.751900839311922
13	25.04475634135182	21.766674703507036	33.805228755688276	19.38334019945286
14	20.002809730019116	29.01482891971576	28.477183283625155	22.50517806663996
15	18.18863540145916	23.557497897448652	35.60231688818604	22.65154981290615
16	20.155826108087275	23.088766585475597	30.290028685824723	26.465378620612405
17	20.785927049536678	24.47028046041603	30.474749449919408	24.26904304012788
18	21.11303278081638	23.275955355668156	30.583911258094588	25.027100605420884
19	21.844701665524425	24.900852601183505	28.178744392405374	25.075701340886695
20	25.271433209110363	23.457448727173315	30.66288745322654	20.608230610489787
21	23.180082811096913	27.997820560768954	28.228674054231583	20.593422573902544
22	20.675246468378198	22.921321864066023	29.976591911394785	26.426839756160998
23	22.61908604038797	26.30571761074228	28.854978063222724	22.220218285647025
24	22.19212098545585	25.238020203477614	28.188236723551043	24.381622087515495
25	20.895468550957684	29.579622622882972	26.81431671352714	22.710592112632206
26	19.9897103130381	24.756948861015186	30.01873786168155	25.234602964265168
27	25.426537900030567	24.651963678544103	26.77976462815691	23.14173379326842
28	20.645060855334975	26.069548411838078	29.662205903850282	23.62318482897667
29	22.823550853265647	22.74495435137952	28.768977543042972	25.662517252311858
30	23.3718279002394	26.454367516483433	28.422507456226114	21.751297127051057
31	25.0174184276523	23.839040439229148	25.257384559014774	25.88615657410378
32	26.216489697973007	25.297821889695314	27.048967139448038	21.436721272883638
33	24.409909234329586	23.03693845742025	27.87840703495646	24.674745273293706
34	22.758243614983456	22.23806386820088	29.147152015886363	25.8565405009293
35	26.501449478965945	21.80198617536892	27.508016273652515	24.188548072012622
36	24.80479020998935	25.08101704632827	28.101476816879646	22.012715926802738
37	23.848912463620643	23.870365132009848	31.321275541490028	20.95944686287948
38	23.091614284819293	23.85916418125796	29.219103885970526	23.83011764795222
39	22.517707943752242	23.551992345384164	32.08256049937256	21.847739211491035
40	23.351894004833497	21.71750442817248	32.57483279258687	22.355768774407156
41	20.92261661803429	23.79214832336955	30.80698104001777	24.47825401857839
42	23.965288443466523	23.2907633922554	30.096195283830195	22.647752880447886
43	23.54781571968007	23.105852781537795	29.716312191380585	23.63001930740155
44	23.51895903299724	22.426391718130922	29.863253477515517	24.191395771356323
45	22.28609506379796	21.760979304819635	29.241885480720125	26.711040150662278
46	25.26972458950414	24.29505202746701	28.254872888193628	22.18035049483522
47	20.909896894299095	23.929597278358813	33.803520136082064	21.35698569126003
48	22.999538672706322	23.210837964008878	28.990148858737026	24.799474504547774
49	22.312483744382913	21.897289180071418	32.691968158924404	23.098258916621262
50	22.32349484851189	21.420584309936004	31.32336385434208	24.932556987210035
51	21.69889945912697	21.62561866268242	28.98616207965585	27.68931979853476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	647.5
2	1100.0
3	3558.0
4	6016.0
5	3955.0
6	1894.0
7	1780.5
8	1667.0
9	1670.5
10	1674.0
11	1672.0
12	1670.0
13	1730.0
14	1790.0
15	1714.0
16	1638.0
17	1604.0
18	1570.0
19	1533.0
20	1496.0
21	1570.5
22	1645.0
23	2000.5
24	2356.0
25	2559.0
26	3706.0
27	4650.0
28	4925.5
29	5201.0
30	6064.5
31	6928.0
32	7887.0
33	8846.0
34	9823.0
35	10800.0
36	11611.5
37	12423.0
38	14091.0
39	15759.0
40	17065.5
41	18372.0
42	20635.0
43	22898.0
44	26361.0
45	29824.0
46	46772.5
47	63721.0
48	56880.0
49	50039.0
50	49361.5
51	48684.0
52	42794.0
53	36904.0
54	33836.5
55	30769.0
56	28038.5
57	25308.0
58	24375.5
59	23443.0
60	22336.5
61	21230.0
62	19154.5
63	17079.0
64	15328.5
65	13578.0
66	11633.5
67	9689.0
68	8517.5
69	7346.0
70	6449.0
71	5552.0
72	4835.0
73	4118.0
74	3403.0
75	2034.5
76	1381.0
77	1216.5
78	1052.0
79	760.0
80	468.0
81	367.5
82	267.0
83	225.5
84	184.0
85	112.5
86	41.0
87	26.5
88	12.0
89	11.5
90	11.0
91	6.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.35169523866221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40526288906588	23.013794310118744
2	8.408305163774983	5.27229241936686
3	3.057282133661006	2.8755293313944037
4	1.8369331296549787	2.3036387061897945
5	1.304742593480795	2.0452946077855807
6	1.0725080852772755	2.017496797836857
7	0.9465361880804493	2.077285987074431
8	0.8396776228108347	2.106025354327172
9	0.8021140647395779	2.263287213392383
>10	8.19330125394145	44.36736864517801
>50	0.07367014583128305	1.5837560180601389
>100	0.05053406697517762	3.0260677539239946
>500	0.0036530650825429612	0.9431527642804804
>1k	0.0036530650825429612	2.2295131120817673
>5k	0.0018265325412714806	3.875496978989394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	7246	1.3756286296301217	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	6685	1.2691246741757334	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	6372	1.2097026812038554	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3800	0.7214171670707236	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2740	0.5201797467825744	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCT	1547	0.2936927256469498	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGTTTGTT	1327	0.2519264686060132	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	1247	0.23673873877294535	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTA	1019	0.1934537087487019	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	987	0.1873786168154748	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	981	0.18623953707799468	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTC	976	0.18529030396342794	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	768	0.1458022063974515	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCC	626	0.11884398594375603	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	603	0.11447751361674903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19250447563413517	0.0
2	0.0	0.0	0.0	1.078138971524905	0.0
3	0.0	0.0	0.0	1.400308690608857	0.0
4	0.0	0.0	0.0	2.013892975864799	0.0
5	0.0	0.0	0.0	4.3548916830092965	0.0
6	0.0	0.0	0.0	5.186799584615589	0.0
7	0.0	0.0	0.0	6.166598005471379	0.0
8	0.0	0.0	0.0	7.397373661818617	0.0
9	0.0	0.0	0.0	7.90673215109513	0.0
10	0.0	0.0	0.0	10.247161318370889	0.0
11	0.0	0.0	0.0	11.41414850941924	0.0
12	0.0	0.0	0.0	13.587322801908337	0.0
13	0.0	0.0	0.0	14.066115984895802	0.0
14	0.0	0.0	0.0	14.273238650494266	0.0
15	0.0	0.0	0.0	14.782217446524953	0.0
16	0.0	0.0	0.0	15.458830810588125	0.0
17	0.0	0.0	0.0	16.18423475674003	0.0
18	0.0	0.0	0.0	16.964124683668064	0.0
19	0.0	0.0	0.0	17.658773476908006	0.0
20	0.0	0.0	0.0	18.122568776685316	0.0
21	0.0	0.0	0.0	18.600222879935302	0.0
22	0.0	0.0	0.0	19.13881774914047	0.0
23	0.0	0.0	0.0	19.64760669854824	0.0
24	0.0	0.0	0.0	20.06204187636808	0.0
25	0.0	0.0	0.0	20.430154478197064	0.0
26	0.0	0.0	0.0	20.776434718391013	0.0
27	0.0	0.0	0.0	21.156317810840623	0.0
28	0.0	0.0	0.0	21.511520842311498	0.0
29	0.0	0.0	0.0	21.86672387378237	0.0
30	0.0	0.0	0.0	22.276223039406464	0.0
31	0.0	0.0	0.0	22.66104214405182	0.0
32	0.0	0.0	0.0	23.022889807324663	0.0
33	0.0	0.0	0.0	23.385876550334984	0.0
34	0.0	0.0	0.0	23.723803539120745	0.0
35	0.0	0.0	0.0	24.122481447238776	0.0
36	0.0	0.0	0.0	24.49515036801768	0.0
37	0.0	0.0	0.0	24.83763367575336	0.0
38	0.0	0.0	0.0	25.15790492860818	0.0
39	0.0	0.0	0.0	25.479694954446302	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	3.887918E-5	45.0	1
ACCGGTT	25	3.887918E-5	45.0	39
TAACGCG	20	7.0298027E-4	45.0	1
GTGTCGA	20	7.0298027E-4	45.0	16
ATGCGCT	20	7.0298027E-4	45.0	18
CGATCGA	90	0.0	45.0	41
TATCGTG	20	7.0298027E-4	45.0	1
CGATCAC	30	2.1632532E-6	44.999996	34
ATAGGGT	105	0.0	42.857147	4
CCGATCG	95	0.0	42.63158	40
TATTAGT	70	0.0	41.785713	30
CGATGAA	945	0.0	41.666668	19
TCGAATG	130	0.0	41.53846	44
ATTGCGG	60	3.6379788E-12	41.249996	2
ATGAATG	1010	0.0	41.21287	21
GATGAAT	1010	0.0	41.21287	20
ACGTAGG	50	1.0786607E-9	40.5	2
CCGATGA	935	0.0	40.42781	18
GGTAATC	45	1.9248546E-8	40.0	8
TACGAGG	45	1.9248546E-8	40.0	2
>>END_MODULE