FastQCFastQC Report
Sat 18 Jun 2016
SRR3551544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677550
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136352.0123976090325435No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTC18690.27584680097409786No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGC16000.2361449339532138No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCG15900.2346690281160062No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC10600.15644601874400413Illumina PCR Primer Index 6 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA8190.1208766880673013No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7570.11172607187661426No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC6880.10154232159988191No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6780.10006641576267433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG351.2109376E-745.0000041
CATAGCG207.0312334E-445.0000041
CTCGAAT207.0312334E-445.00000443
CAGTACG207.0312334E-445.0000041
TATCGCG351.2109376E-745.0000041
TATCACG253.889104E-545.01
GTTAACG253.889104E-545.01
ACATCGG253.889104E-545.02
CGATCTC253.889104E-545.011
CGTTTTT54000.042.5833321
ATAACGC800.039.37500411
ACGGGCT1900.039.0789455
TTTAGCG356.2444924E-638.571431
TCGTAAG356.2444924E-638.571431
ATAACGG356.2444924E-638.571432
CGTTTAT700.038.5714339
CACTAGG659.094947E-1238.0769272
TAGGGTC1250.037.8000035
GGCGATA2450.037.653068
TGGGCGA3000.037.5000046