FastQCFastQC Report
Sat 18 Jun 2016
SRR3551539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774846
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98161.2668323770142713No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC19780.2552765323690127No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT13050.16842056357005133No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC11690.15086868874589274No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC11640.15022339923029868No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC10540.1360270298872292No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC8960.11563588119445671No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7810.10079422233579317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCG207.031861E-445.00000415
CGTTTTT38350.042.594531
CGGTCTA750.042.00000431
TACGAAT1250.041.39999812
TACCGGT556.002665E-1140.90909240
CGGTATT501.0804797E-940.543
GCGTTTA850.039.70588332
TAGACGG750.039.0000042
TCGCGTA356.2456547E-638.57142630
CGAATAT1350.038.33333214
GCTACGA1350.038.33333210
CTCACGA900.037.524
GCCCGAA900.037.525
GATCGCC301.1395785E-437.49999630
ATAAGCG301.1395785E-437.4999961
ATAACGC552.746674E-936.8181811
TTACACG1100.036.8181834
CCCTCGT2150.036.62790714
TGTTCGG250.00210668636.02
GTGCGTA250.00210668636.016