##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551539_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 774846 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36193127408543 33.0 31.0 34.0 31.0 34.0 2 32.48904040286715 34.0 31.0 34.0 31.0 34.0 3 32.48709550026715 34.0 31.0 34.0 31.0 34.0 4 36.008319072435036 37.0 35.0 37.0 35.0 37.0 5 36.09588485970115 37.0 35.0 37.0 35.0 37.0 6 36.10639404475212 37.0 36.0 37.0 35.0 37.0 7 36.22614945421413 37.0 37.0 37.0 35.0 37.0 8 36.140510501441575 37.0 37.0 37.0 35.0 37.0 9 37.98703097131559 39.0 38.0 39.0 35.0 39.0 10 37.69970548986508 39.0 37.0 39.0 35.0 39.0 11 37.64952519597443 39.0 37.0 39.0 35.0 39.0 12 37.58002235282882 39.0 37.0 39.0 35.0 39.0 13 37.564081120635585 39.0 37.0 39.0 35.0 39.0 14 38.86899074138603 40.0 38.0 41.0 35.0 41.0 15 38.922631077659304 40.0 38.0 41.0 35.0 41.0 16 38.940413966130045 40.0 38.0 41.0 35.0 41.0 17 38.88439509270229 40.0 38.0 41.0 35.0 41.0 18 38.769792965311815 40.0 38.0 41.0 35.0 41.0 19 38.67201482617191 40.0 37.0 41.0 35.0 41.0 20 38.551283738962326 40.0 37.0 41.0 35.0 41.0 21 38.43752952199534 40.0 37.0 41.0 35.0 41.0 22 38.434145623775564 40.0 37.0 41.0 35.0 41.0 23 38.40948394906859 40.0 37.0 41.0 35.0 41.0 24 38.32949515129458 40.0 37.0 41.0 35.0 41.0 25 38.217947824470926 40.0 36.0 41.0 34.0 41.0 26 38.21842921044956 40.0 37.0 41.0 34.0 41.0 27 38.220248926883535 40.0 36.0 41.0 34.0 41.0 28 38.14914963747635 40.0 36.0 41.0 34.0 41.0 29 38.09450264955875 40.0 36.0 41.0 34.0 41.0 30 37.95439480877491 40.0 36.0 41.0 34.0 41.0 31 37.8999710910297 40.0 36.0 41.0 34.0 41.0 32 37.73854546580869 40.0 36.0 41.0 33.0 41.0 33 37.590681245047406 40.0 36.0 41.0 33.0 41.0 34 37.39942388552048 40.0 36.0 41.0 33.0 41.0 35 37.26992847611009 40.0 35.0 41.0 33.0 41.0 36 37.191053448039995 40.0 35.0 41.0 33.0 41.0 37 37.125690782426446 40.0 35.0 41.0 33.0 41.0 38 36.96006303187988 40.0 35.0 41.0 32.0 41.0 39 36.94680104175539 40.0 35.0 41.0 32.0 41.0 40 36.858897122783105 40.0 35.0 41.0 32.0 41.0 41 36.752273354963435 39.0 35.0 41.0 31.0 41.0 42 36.67302276839527 39.0 35.0 41.0 31.0 41.0 43 36.65467073457177 39.0 35.0 41.0 31.0 41.0 44 36.59819112442989 39.0 35.0 41.0 31.0 41.0 45 36.48084135428201 39.0 35.0 41.0 31.0 41.0 46 36.416184635398515 39.0 35.0 41.0 31.0 41.0 47 36.36831964028981 39.0 35.0 41.0 31.0 41.0 48 36.3039313618448 39.0 35.0 41.0 31.0 41.0 49 36.25325806676423 39.0 35.0 41.0 31.0 41.0 50 36.16693252594709 38.0 35.0 41.0 31.0 41.0 51 35.3529256136058 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 11.0 10 11.0 11 19.0 12 22.0 13 21.0 14 30.0 15 48.0 16 50.0 17 90.0 18 165.0 19 286.0 20 472.0 21 752.0 22 1104.0 23 1728.0 24 2773.0 25 4562.0 26 7096.0 27 8822.0 28 9080.0 29 8897.0 30 9547.0 31 10887.0 32 13661.0 33 19180.0 34 39254.0 35 55923.0 36 53267.0 37 78812.0 38 144849.0 39 303069.0 40 353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72016116750942 19.97003275489581 23.496023726004907 14.813782351589863 2 35.56564788357945 23.640955751207336 23.165119262408272 17.628277102804944 3 31.677520436318957 22.267779662023163 29.043577691567098 17.01112221009078 4 28.65266130302021 23.939079507411794 28.173469308740057 19.234789880827933 5 24.87462024712007 28.655758692695066 26.553921682502075 19.91569937768279 6 25.915214119967068 33.13445510462724 25.90011434530216 15.05021643010353 7 75.42556843553429 6.586082911959279 12.667291306917761 5.321057345588672 8 75.74343805091593 6.175678780041453 12.481060752717314 5.599822416325308 9 69.10418328287169 8.26615869475999 14.932386564556055 7.697271457812262 10 39.48139372210736 22.117298146986627 22.30959442263366 16.091713708272355 11 29.87329095071795 22.988955224651093 26.364722796529893 20.77303102810107 12 25.82448641407454 20.72953851475003 32.0125547528154 21.433420318360035 13 24.628635883775615 21.045472261584884 33.62977933679725 20.696112517842256 14 21.251448674962507 24.044132640550508 32.396760130400104 22.307658554086878 15 20.877309813821068 22.977598129176634 34.60636048969731 21.538731567304986 16 23.142921303071837 22.36715424742465 32.587894884919066 21.902029564584446 17 23.01412151575926 22.670311261850742 30.16070290096355 24.15486432142645 18 23.088716983761934 22.821179950596633 31.516972404839155 22.573130660802278 19 24.525647677086802 24.232428121200858 29.19896340692215 22.04296079479019 20 24.7938299997677 24.203519150902245 29.43436502221087 21.568285827119198 21 24.228943557816653 24.60192089783002 29.890197536026513 21.27893800832682 22 22.9053257034301 22.898485634564803 29.623434850279928 24.572753811725168 23 22.854605947504407 24.052392346350114 29.763333617260717 23.32966808888476 24 23.617467212839713 23.516156758891444 30.00802740157399 22.858348626694852 25 24.00038201139323 23.961664640457588 28.270391793982284 23.767561554166893 26 22.58900478288589 24.127245930159024 28.900581534911453 24.383167752043633 27 23.733877441452883 23.34592938467773 29.331376815522052 23.588816358347337 28 21.881380300085436 24.086592690676596 30.07900924828934 23.95301776094863 29 24.070331394883627 23.15118100887144 28.732160971341404 24.04632662490353 30 24.04710097232224 22.643983449614506 28.66453463010714 24.64438094795611 31 23.927464296131102 24.139248315149075 27.495528143656934 24.43775924506289 32 23.571393541426296 24.40639817460502 27.41009181179228 24.612116472176407 33 22.787108664173267 23.64379502507595 27.448938240631044 26.120158070119743 34 22.238354460112074 23.53732225500293 29.186315732416507 25.03800755246849 35 22.65430808186401 23.689094349070654 28.16946851374338 25.48712905532196 36 22.536091042607175 24.72955916401453 28.5174086205517 24.2169411728266 37 21.729221032308356 25.61012123699419 29.296531181679974 23.364126549017485 38 21.4503269036686 25.908374051101767 27.82294804386936 24.81835100136027 39 22.304561164412025 23.487764020205304 29.017637053040218 25.190037762342456 40 22.502665045699406 23.29662926568634 30.62569852590063 23.575007162713625 41 20.737411046840275 24.588369818002544 29.39048533515047 25.283733800006715 42 21.17336864357563 24.901335233065666 29.458369792190965 24.46692633116774 43 22.39722473885133 22.339277740350987 29.877162687811516 25.38633483298617 44 22.572356313383562 22.768782441930398 28.751132483099866 25.907728761586174 45 22.688379368287375 22.360572294365593 28.34498726198496 26.60606107536207 46 22.66837539330396 23.50892951631679 29.160375093889623 24.662319996489625 47 21.186274433887508 23.719552014206695 30.849226814102416 24.24494673780338 48 20.59789945356884 23.58094382625709 30.33970621258934 25.481450507584736 49 21.52324461893073 22.565903418227624 31.14657622288816 24.764275739953487 50 20.697015923164088 21.888994716369446 31.227237412337423 26.18675194812905 51 20.66178311561265 22.681797415228317 29.426879663829975 27.22953980532906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 316.0 1 956.0 2 1596.0 3 8146.5 4 14697.0 5 9368.0 6 4039.0 7 3794.5 8 3550.0 9 3451.0 10 3352.0 11 3289.5 12 3227.0 13 3155.0 14 3083.0 15 2917.0 16 2751.0 17 2652.5 18 2554.0 19 2422.0 20 2290.0 21 2342.0 22 2394.0 23 2606.0 24 2818.0 25 3380.5 26 4737.0 27 5531.0 28 6596.0 29 7661.0 30 8691.0 31 9721.0 32 11056.5 33 12392.0 34 13656.0 35 14920.0 36 16328.5 37 17737.0 38 19177.0 39 20617.0 40 23014.5 41 25412.0 42 28482.0 43 31552.0 44 36131.0 45 40710.0 46 48886.0 47 57062.0 48 63961.0 49 70860.0 50 69802.0 51 68744.0 52 61804.0 53 54864.0 54 50732.5 55 46601.0 56 44223.5 57 41846.0 58 40823.5 59 39801.0 60 39616.5 61 39432.0 62 36473.0 63 33514.0 64 30452.5 65 27391.0 66 23147.5 67 18904.0 68 15920.0 69 12936.0 70 11321.0 71 9706.0 72 8645.5 73 7585.0 74 5832.0 75 3201.5 76 2324.0 77 1804.5 78 1285.0 79 906.0 80 527.0 81 439.0 82 351.0 83 232.5 84 114.0 85 79.0 86 44.0 87 25.5 88 7.0 89 5.0 90 3.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 774846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.49848238196108 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9728551812731 23.71507048470001 2 8.80362700045509 5.722090339432933 3 3.2425858483984547 3.161373571985205 4 1.8770946524269325 2.440109099646801 5 1.3446002423420294 2.1848733643266516 6 1.1184492417237768 2.180874178636673 7 0.9522788860383007 2.166333302044047 8 0.8462039203613025 2.2000274557926547 9 0.7880020296404073 2.304798306949651 >10 7.9240823830293365 46.01195980843738 >50 0.07509655094151813 1.6886348563664149 >100 0.047135068678494756 2.8673559644039592 >500 0.005592296283889205 1.216367502103635 >1k 0.001997248672817573 0.8658667277514669 >5k 3.9944973456351463E-4 1.2742650374225486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9816 1.2668323770142713 No Hit GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC 1978 0.2552765323690127 No Hit CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT 1305 0.16842056357005133 No Hit CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC 1169 0.15086868874589274 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 1164 0.15022339923029868 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC 1054 0.1360270298872292 No Hit GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC 896 0.11563588119445671 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 781 0.10079422233579317 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.290579031188133E-4 0.0 0.0 0.1730666480823286 0.0 2 1.290579031188133E-4 0.0 0.0 0.7489230117984735 0.0 3 2.581158062376266E-4 0.0 0.0 1.0897649339352593 0.0 4 2.581158062376266E-4 0.0 0.0 1.573732070630809 0.0 5 2.581158062376266E-4 0.0 0.0 2.825593730883298 0.0 6 2.581158062376266E-4 0.0 0.0 3.788365688149645 0.0 7 2.581158062376266E-4 0.0 0.0 4.622079742297179 0.0 8 2.581158062376266E-4 0.0 0.0 5.797151950193974 0.0 9 2.581158062376266E-4 0.0 0.0 6.349648833445614 0.0 10 2.581158062376266E-4 0.0 0.0 7.375271989530823 0.0 11 2.581158062376266E-4 0.0 0.0 8.594611058197371 0.0 12 2.581158062376266E-4 0.0 0.0 9.573644311256688 0.0 13 2.581158062376266E-4 0.0 0.0 9.985339022205704 0.0 14 2.581158062376266E-4 0.0 0.0 10.163438928509665 0.0 15 2.581158062376266E-4 0.0 0.0 10.468918985191896 0.0 16 2.581158062376266E-4 0.0 0.0 11.037677164236506 0.0 17 2.581158062376266E-4 0.0 0.0 11.689290517083395 0.0 18 2.581158062376266E-4 0.0 0.0 12.393559494402759 0.0 19 3.8717370935643984E-4 0.0 0.0 12.839970781290734 0.0 20 3.8717370935643984E-4 0.0 0.0 13.26444222464851 0.0 21 3.8717370935643984E-4 0.0 0.0 13.791901874695101 0.0 22 3.8717370935643984E-4 0.0 0.0 14.345173105365454 0.0 23 3.8717370935643984E-4 0.0 0.0 14.903606652160558 0.0 24 3.8717370935643984E-4 0.0 0.0 15.3710543772569 0.0 25 3.8717370935643984E-4 0.0 0.0 15.790621620296163 0.0 26 3.8717370935643984E-4 0.0 0.0 16.19431474125181 0.0 27 3.8717370935643984E-4 0.0 0.0 16.5694860656182 0.0 28 3.8717370935643984E-4 0.0 0.0 16.980019255439146 0.0 29 3.8717370935643984E-4 0.0 0.0 17.379324407688753 0.0 30 3.8717370935643984E-4 0.0 0.0 17.819669973130143 0.0 31 3.8717370935643984E-4 0.0 0.0 18.233687726335297 0.0 32 3.8717370935643984E-4 0.0 0.0 18.670161554683123 0.0 33 3.8717370935643984E-4 0.0 0.0 19.089728797722387 0.0 34 3.8717370935643984E-4 0.0 0.0 19.487743370940805 0.0 35 3.8717370935643984E-4 0.0 0.0 19.910795177364275 0.0 36 3.8717370935643984E-4 0.0 0.0 20.316940398479183 0.0 37 3.8717370935643984E-4 0.0 0.0 20.73908879958082 0.0 38 3.8717370935643984E-4 0.0 0.0 21.17517545421929 0.0 39 3.8717370935643984E-4 0.0 0.0 21.59590421838662 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATTCG 20 7.031861E-4 45.000004 15 CGTTTTT 3835 0.0 42.59453 1 CGGTCTA 75 0.0 42.000004 31 TACGAAT 125 0.0 41.399998 12 TACCGGT 55 6.002665E-11 40.909092 40 CGGTATT 50 1.0804797E-9 40.5 43 GCGTTTA 85 0.0 39.705883 32 TAGACGG 75 0.0 39.000004 2 TCGCGTA 35 6.2456547E-6 38.571426 30 CGAATAT 135 0.0 38.333332 14 GCTACGA 135 0.0 38.333332 10 CTCACGA 90 0.0 37.5 24 GCCCGAA 90 0.0 37.5 25 GATCGCC 30 1.1395785E-4 37.499996 30 ATAAGCG 30 1.1395785E-4 37.499996 1 ATAACGC 55 2.746674E-9 36.81818 11 TTACACG 110 0.0 36.81818 34 CCCTCGT 215 0.0 36.627907 14 TGTTCGG 25 0.002106686 36.0 2 GTGCGTA 25 0.002106686 36.0 16 >>END_MODULE