FastQCFastQC Report
Sat 18 Jun 2016
SRR3551538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256990
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.7786295186583135No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC12250.4766722440561889TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC8660.3369780925327834TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT8610.33503249153663567TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC5660.2202420327639208No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC3640.1416397525195533TruSeq Adapter, Index 15 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA3610.14047239192186467No Hit
ACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC3510.13658118992956925TruSeq Adapter, Index 14 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2800.10895365578427177No Hit
GCCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG2710.10545157399120589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.2070086E-745.00000425
CGACGGT302.1589021E-645.00000428
CACGACG302.1589021E-645.00000426
TACGGGT302.1589021E-645.0000044
CGCGAGG302.1589021E-645.0000042
ACGCATG253.8823255E-545.01
TTATGCG207.023053E-445.01
GGGTAAC207.023053E-445.07
CCGTGAT207.023053E-445.027
GTGATCC207.023053E-445.029
ACCTAGT253.8823255E-545.020
CACTAGG207.023053E-445.02
TCGAGTA253.8823255E-545.026
GACGAGG207.023053E-445.02
ATTGCGG406.7793735E-945.02
TAGTCGA253.8823255E-545.023
CCCGATG207.023053E-445.015
TAGGGTG650.044.9999965
AGGGATA950.042.631586
GTGTTAG650.041.538461
Produced by FastQC (version 0.11.5)