##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256990
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.344632086851625	33.0	31.0	34.0	31.0	34.0
2	32.45614226234484	34.0	31.0	34.0	31.0	34.0
3	32.47455543017238	34.0	31.0	34.0	31.0	34.0
4	35.99708548970777	37.0	35.0	37.0	35.0	37.0
5	36.091431573212965	37.0	35.0	37.0	35.0	37.0
6	36.10828047783961	37.0	36.0	37.0	35.0	37.0
7	36.23212576364839	37.0	37.0	37.0	35.0	37.0
8	36.144098992178684	37.0	37.0	37.0	35.0	37.0
9	38.011665823572905	39.0	39.0	39.0	35.0	39.0
10	37.696564068640804	39.0	37.0	39.0	35.0	39.0
11	37.683905988559864	39.0	37.0	39.0	35.0	39.0
12	37.62143274057356	39.0	37.0	39.0	35.0	39.0
13	37.58243900540877	39.0	37.0	39.0	35.0	39.0
14	38.8605548854041	40.0	38.0	41.0	35.0	41.0
15	38.928199540838165	40.0	38.0	41.0	35.0	41.0
16	38.959352503988484	40.0	38.0	41.0	35.0	41.0
17	38.93682244445309	40.0	38.0	41.0	35.0	41.0
18	38.8486361337017	40.0	38.0	41.0	35.0	41.0
19	38.77692906338768	40.0	38.0	41.0	35.0	41.0
20	38.67799525273357	40.0	38.0	41.0	35.0	41.0
21	38.58354410677458	40.0	38.0	41.0	35.0	41.0
22	38.581045955095526	40.0	38.0	41.0	35.0	41.0
23	38.534063582240556	40.0	37.0	41.0	35.0	41.0
24	38.46880034242577	40.0	37.0	41.0	35.0	41.0
25	38.36120471613682	40.0	37.0	41.0	35.0	41.0
26	38.36638390598856	40.0	37.0	41.0	35.0	41.0
27	38.35916572629285	40.0	37.0	41.0	35.0	41.0
28	38.310381726915445	40.0	37.0	41.0	35.0	41.0
29	38.22986886649286	40.0	37.0	41.0	34.0	41.0
30	38.082365072570916	40.0	37.0	41.0	34.0	41.0
31	38.052503988482044	40.0	37.0	41.0	34.0	41.0
32	37.919592202031204	40.0	36.0	41.0	34.0	41.0
33	37.80776294797463	40.0	36.0	41.0	34.0	41.0
34	37.65745359741624	40.0	36.0	41.0	33.0	41.0
35	37.558103428148954	40.0	36.0	41.0	33.0	41.0
36	37.477512743686525	40.0	36.0	41.0	33.0	41.0
37	37.42510992645628	40.0	36.0	41.0	33.0	41.0
38	37.276567181602395	40.0	35.0	41.0	33.0	41.0
39	37.27924821977509	40.0	35.0	41.0	33.0	41.0
40	37.21267364488891	40.0	35.0	41.0	33.0	41.0
41	37.133849566130976	40.0	35.0	41.0	33.0	41.0
42	37.05853924277209	40.0	35.0	41.0	32.0	41.0
43	37.057799914393556	40.0	35.0	41.0	33.0	41.0
44	36.98349352114868	39.0	35.0	41.0	32.0	41.0
45	36.87031791120277	39.0	35.0	41.0	32.0	41.0
46	36.79954083816491	39.0	35.0	41.0	32.0	41.0
47	36.75237168761431	39.0	35.0	41.0	32.0	41.0
48	36.71897350091443	39.0	35.0	41.0	32.0	41.0
49	36.673855013813764	39.0	35.0	41.0	32.0	41.0
50	36.5965134830149	39.0	35.0	41.0	31.0	41.0
51	35.78369197245029	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	0.0
11	3.0
12	7.0
13	3.0
14	9.0
15	11.0
16	15.0
17	26.0
18	38.0
19	82.0
20	116.0
21	192.0
22	312.0
23	485.0
24	777.0
25	1304.0
26	1918.0
27	2407.0
28	2495.0
29	2524.0
30	2878.0
31	3296.0
32	4229.0
33	6149.0
34	12377.0
35	18947.0
36	16073.0
37	25588.0
38	50856.0
39	103739.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42581423401688	21.108992567804194	21.540526868749758	13.924666329429161
2	34.69084400171213	24.763220358768823	22.859644344137905	17.686291295381142
3	31.762325382310596	23.9067668002646	27.041130005058562	17.28977781236624
4	29.108136503365888	25.902564302112925	26.71582551850267	18.273473676018522
5	24.937546208023658	29.08362193081443	25.60761119109693	20.371220670064982
6	25.340674734425463	34.606794038678544	25.69321763492743	14.359313591968558
7	75.4675279193743	6.8827580839721385	12.216428654811471	5.433285341842095
8	74.77255924355033	6.664850772403595	11.995408381649092	6.567181602396981
9	68.34623915327444	8.711623020350986	14.780730767734154	8.161407058640414
10	37.28938869216701	22.674034009105412	23.393906377680064	16.64267092104751
11	28.35441067745827	23.802093466671856	26.327483559671585	21.516012296198294
12	25.762870150589517	21.646367562940192	32.073232421495	20.51752986497529
13	24.00210124907584	23.297404568271137	33.01645978442741	19.68403439822561
14	20.16226312307872	27.14424685785439	31.271255690882917	21.422234328183976
15	19.124090431534302	24.91886843846064	35.513444102883376	20.443597027121676
16	20.591851823028133	24.08770769290634	32.75964045293591	22.560800031129617
17	21.02027316237986	23.358496439550176	29.88131833923499	25.739912058834975
18	20.619090236974202	24.691233121911356	32.443674851161525	22.246001789952917
19	22.765866376123583	24.91147515467528	29.985213432429276	22.337445036771857
20	23.181057628701506	25.821238180473948	29.864975290867353	21.132728899957197
21	22.577921319895715	26.03291956885482	30.191058017821703	21.19810109342776
22	20.82260010117125	24.99708159850578	28.647807307677343	25.53251099264563
23	20.612475193587297	25.85003307521693	29.68675823962022	23.85073349157555
24	21.925755865987004	24.882291139733066	29.766527880462274	23.425425113817656
25	20.872796606871862	26.56834896299467	28.436125919296472	24.122728510836996
26	19.62566636834118	28.05517724425075	28.72563134752325	23.59352503988482
27	21.895793610646326	27.404957391338186	29.062220319856802	21.637028678158686
28	19.493754620802367	26.93918051286042	30.337367212732012	23.2296976536052
29	19.99221759601541	27.237635705669483	29.61788396435659	23.15226273395852
30	21.381765827464104	27.73298571928869	28.008093700143977	22.877154753103234
31	22.415658196816995	29.246274174092378	24.99396863691194	23.344098992178683
32	21.031946768356747	28.827191719522162	26.45044554262812	23.690415969492975
33	21.18175804506012	26.720494960893422	25.707615082298922	26.390131911747538
34	20.870461885676487	26.291684501342466	27.430639324487334	25.40721428849372
35	20.861901241293435	26.146153546830615	27.224405618895673	25.76753959298027
36	21.679831900073932	27.920930775516556	25.669870422973656	24.729366901435853
37	21.404334799019416	28.252072065060897	27.56955523561228	22.774037900307405
38	19.904276430989533	27.851278259854467	26.52048717848944	25.723958130666563
39	22.52305537180435	23.741390715592043	28.007315459745513	25.728238452858086
40	22.72812171679832	24.133234756216197	28.359469240048252	24.779174286937238
41	20.471224561266975	25.74925094361648	26.154325071014433	27.625199424102103
42	20.24125452352232	26.994046460951786	26.669131094595123	26.095567920930772
43	21.63235923576793	25.644577610023738	27.794077590567728	24.92898556364061
44	20.700416358613175	25.78349352114868	27.971516401416395	25.544573718821745
45	21.671660375890113	23.793143702089576	28.07229853301685	26.462897389003466
46	22.730456437993695	24.738316666018136	27.878127553601306	24.653099342386863
47	20.873963967469553	25.28658702673256	29.517101832756136	24.322347173041752
48	19.951749095295536	24.235573368613565	28.86804934044126	26.944628195649635
49	20.99614771002763	23.35810731935095	30.665784660881744	24.97996030973968
50	20.574730534262034	22.875598272306316	30.03969026032141	26.50998093311024
51	20.298455192809058	23.409860305848476	26.761741702011747	29.529942799330712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	428.0
2	661.0
3	1912.0
4	3163.0
5	2068.5
6	974.0
7	972.5
8	971.0
9	981.0
10	991.0
11	956.0
12	921.0
13	902.0
14	883.0
15	922.0
16	961.0
17	945.5
18	930.0
19	927.5
20	925.0
21	908.0
22	891.0
23	967.5
24	1044.0
25	1295.0
26	1766.0
27	1986.0
28	2376.0
29	2766.0
30	3227.0
31	3688.0
32	3987.5
33	4287.0
34	4642.0
35	4997.0
36	5486.0
37	5975.0
38	6606.5
39	7238.0
40	8638.5
41	10039.0
42	11602.5
43	13166.0
44	15094.0
45	17022.0
46	19838.0
47	22654.0
48	26014.0
49	29374.0
50	28398.0
51	27422.0
52	23164.0
53	18906.0
54	16380.0
55	13854.0
56	12725.5
57	11597.0
58	11260.0
59	10923.0
60	9872.0
61	8821.0
62	8242.5
63	7664.0
64	6859.0
65	6054.0
66	5025.0
67	3996.0
68	3566.5
69	3137.0
70	2713.5
71	2290.0
72	2043.5
73	1797.0
74	1447.5
75	860.5
76	623.0
77	428.0
78	233.0
79	210.5
80	188.0
81	128.5
82	69.0
83	57.5
84	46.0
85	26.0
86	6.0
87	10.5
88	15.0
89	8.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	256990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.489539838245825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.96628587228322	28.814050528023614
2	9.941819117936621	7.8519572424478445
3	3.739578787534221	4.430227365257749
4	2.144576054565242	3.3875328617160876
5	1.5296917990051264	3.020341261852543
6	1.2527473852325408	2.968225066583923
7	0.9997983844844462	2.7637104694018646
8	0.961806045549431	3.038502252191195
9	0.7928401186166439	2.817800230452436
>10	5.562878209306334	34.31501880753418
>50	0.06098770143200664	1.6657277808982014
>100	0.04199153213351277	2.7479178371774076
>500	0.0029993951523937692	0.9053125862999707
>1k	0.0019995967682625124	1.2736757101629765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2001	0.7786295186583135	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	1225	0.4766722440561889	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	866	0.3369780925327834	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	861	0.33503249153663567	TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC	566	0.2202420327639208	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	364	0.1416397525195533	TruSeq Adapter, Index 15 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	361	0.14047239192186467	No Hit
ACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	351	0.13658118992956925	TruSeq Adapter, Index 14 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	280	0.10895365578427177	No Hit
GCCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG	271	0.10545157399120589	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.78240398459084E-4	0.0	0.0	0.34514961671660377	0.0
2	7.78240398459084E-4	0.0	0.0	1.4848826802599322	0.0
3	7.78240398459084E-4	0.0	0.0	2.0638935367134907	0.0
4	7.78240398459084E-4	0.0	0.0	2.725008755204483	0.0
5	7.78240398459084E-4	0.0	0.0	4.52896999883264	0.0
6	7.78240398459084E-4	0.0	0.0	5.484260087941165	0.0
7	7.78240398459084E-4	0.0	0.0	6.463675629401922	0.0
8	7.78240398459084E-4	0.0	0.0	7.908089808941982	0.0
9	7.78240398459084E-4	0.0	0.0	8.468033775633293	0.0
10	7.78240398459084E-4	0.0	0.0	9.766917000661504	0.0
11	7.78240398459084E-4	0.0	0.0	11.692672866648508	0.0
12	7.78240398459084E-4	0.0	0.0	13.180279388303047	0.0
13	7.78240398459084E-4	0.0	0.0	13.801315226273395	0.0
14	7.78240398459084E-4	0.0	0.0	14.05307599517491	0.0
15	7.78240398459084E-4	0.0	0.0	14.524300556441885	0.0
16	7.78240398459084E-4	0.0	0.0	15.445737188217441	0.0
17	7.78240398459084E-4	0.0	0.0	16.548503832833962	0.0
18	7.78240398459084E-4	0.0	0.0	17.820148643916106	0.0
19	7.78240398459084E-4	0.0	0.0	18.528347406513873	0.0
20	7.78240398459084E-4	0.0	0.0	19.21709015915016	0.0
21	7.78240398459084E-4	0.0	0.0	20.035020817930658	0.0
22	7.78240398459084E-4	0.0	0.0	20.89030701583719	0.0
23	7.78240398459084E-4	0.0	0.0	21.720300400793807	0.0
24	7.78240398459084E-4	0.0	0.0	22.408654033230864	0.0
25	7.78240398459084E-4	0.0	0.0	23.020740106618934	0.0
26	7.78240398459084E-4	0.0	0.0	23.563173664344916	0.0
27	7.78240398459084E-4	0.0	0.0	24.102494260477062	0.0
28	7.78240398459084E-4	0.0	0.0	24.66477294836375	0.0
29	7.78240398459084E-4	0.0	0.0	25.20059146270283	0.0
30	7.78240398459084E-4	0.0	0.0	25.77882407875793	0.0
31	7.78240398459084E-4	0.0	0.0	26.34732868983229	0.0
32	7.78240398459084E-4	0.0	0.0	26.897933771742093	0.0
33	7.78240398459084E-4	0.0	0.0	27.446593252655745	0.0
34	7.78240398459084E-4	0.0	0.0	27.99408537297171	0.0
35	7.78240398459084E-4	0.0	0.0	28.566092065839136	0.0
36	7.78240398459084E-4	0.0	0.0	29.068446243044477	0.0
37	7.78240398459084E-4	0.0	0.0	29.587143468617455	0.0
38	7.78240398459084E-4	0.0	0.0	30.099614771002763	0.0
39	7.78240398459084E-4	0.0	0.0	30.60313630880579	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2070086E-7	45.000004	25
CGACGGT	30	2.1589021E-6	45.000004	28
CACGACG	30	2.1589021E-6	45.000004	26
TACGGGT	30	2.1589021E-6	45.000004	4
CGCGAGG	30	2.1589021E-6	45.000004	2
ACGCATG	25	3.8823255E-5	45.0	1
TTATGCG	20	7.023053E-4	45.0	1
GGGTAAC	20	7.023053E-4	45.0	7
CCGTGAT	20	7.023053E-4	45.0	27
GTGATCC	20	7.023053E-4	45.0	29
ACCTAGT	25	3.8823255E-5	45.0	20
CACTAGG	20	7.023053E-4	45.0	2
TCGAGTA	25	3.8823255E-5	45.0	26
GACGAGG	20	7.023053E-4	45.0	2
ATTGCGG	40	6.7793735E-9	45.0	2
TAGTCGA	25	3.8823255E-5	45.0	23
CCCGATG	20	7.023053E-4	45.0	15
TAGGGTG	65	0.0	44.999996	5
AGGGATA	95	0.0	42.63158	6
GTGTTAG	65	0.0	41.53846	1
>>END_MODULE